An Aligned Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Scaffold Fixed with Fibronectin to Enhance the Attachment and Growth of Human Endothelial Progenitor Cells

Chien-Ning Hsu,Ya-Ting Lin,Yu-Hsu Chen,Tsung-Yu Tseng,Hsing-Fen Tsai,Shinn-Gwo Hong,Chao-Ling Yao 한국생물공학회 2023 Biotechnology and Bioprocess Engineering Vol.28 No.3

Repair and regeneration of vascular tissue is a crucial current research focus in the fields of biomedical engineering and regenerative medicine. Numerous studies revealed that cells are required to grow on an appropriate extracellular matrix to maintain or enhance functionality. In the present study, various surface modification methods were evaluated to fix fibronectin on the surface of a bio-based and aligned poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) film for vascular tissue engineering. After chemical modification, the properties of the fibronectin-fixed PHBV films were examined and compared with the original films, including -NH2 group expression, contact angle, mechanical properties, and fibronectin binding amount. Then, cytotoxicity and biocompatibility were measured by culture with L929 cells and endothelial progenitor cells (EPCs) of the fibronectinfixed PHBV films. In addition, cell morphology, cell growth kinetics, acetylated low-density lipoprotein uptake ability, lectin binding ability and specific gene expressions of cultured EPCs on fibronectin-fixed PHBV films were also analyzed. Taken together, our data demonstrated that the surface of the aligned PHBV films could be successfully modified to immobilize fibronectin. Importantly, EPCs cultured on the fibronectin-fixed PHBV films showed excellent cell biocompatibility, a rapid proliferation rate, an aligned growth direction and correct cell functions. We believed that fibronectin-fixed PHBV films can serve as a potential scaffold for vascular tissue engineering.

Amphiphilically modified chitosan copolymer for enhanced oil recovery in harsh reservoir condition

Wan-Fen Pu,Rui Liu,Qin Peng,Dai-Jun Du,Qi-Ning Zhao 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.37 No.-

A novel amphiphilically grafting natural chitosan copolymer (PAMCS) was prepared by using one stepwater-free radical polymerization strategy and the basic parameters for PAMCS were systematicallycharacterized. The rigid D-glucosamine unit, intermolecular association and hydrogen bondingsynergistically endowed PAMCS solution with rheological properties, super-high salt resistance andtemperature tolerance in harsh reservoir condition. PAMCS solution exhibited viscoelastic behaviorand formulated the unique displacement mechanism in comparison to that for partially hydrolyzedpolyacrylamide. Moreover, the mass concentration, injective rate and polymer solution slug related to oilrecovery efficiency for PAMCS were investigated from the economic point.

Tissue distribution of sialic acid-linked influenza virus receptors in beagle dogs

Zhang-Yong Ning,Xin-Tao Wu,Yan-Fen Cheng,Wen-Bao Qi,Yu-Fu An,Heng Wang,Gui-Hong Zhang,Shou-Jun Li 대한수의학회 2012 Journal of Veterinary Science Vol.13 No.3

Reports of influenza A virus infections in dogs has received considerable attention from veterinarians, virologists, and epidemiologists. Interaction between influenza viral hemagglutinin and cell oligosaccharides containing sialic acid residues results in infection. Sialic acids have an α-2,3-linkage to the penultimate galactose in the avian influenza virus receptor and an α-2,6-linkage in the human receptor. To date,there are no detailed data on the tissue distribution or histological features of either type of sialic acid-linked influenza virus receptors in beagle dogs, which are common laboratory animals and pets. We conducted the current study to visualize the in situ tissue distribution of both sialic acid-linked influenza virus receptors in various organs of beagle dogs using Maackia amurensis lectin II and Sambucus nigra agglutinin. Both α-2,3- and α-2,6-sialic acid-linked receptors were detected in the endothelial cells of the respiratory tract and other organs. Endothelial cells of most gastrointestinal organs were negative for α-2,3-sialic acid-linked receptors in the dogs. Our results suggested that these canine organs may be affected by influenza virus infection. The findings from our study will also help evaluate the occurrence and development of influenza virus infections in dogs.

Securing the AES Cryptographic Circuit Against both Power and Fault Attacks

Jinbao Zhang,Ning Wu,Fang Zhou,Fen Ge,Xiaoqiang Zhang 대한전기학회 2019 Journal of Electrical Engineering & Technology Vol.14 No.5

Aiming to protect cryptographic circuits against physical attacks, researchers have proposed a variety of mature and effective countermeasures. However, most of these defensive technologies are used for specific and single attack, thus it is hard to thwart combined attack, such as combined power and fault attacks. In this paper, we propose a dual complementary infection countermeasure for Advanced Encryption Standard (AES) cryptographic circuit to defend against both power and fault attacks. According to the target AES circuit, we first design and construct a dual complementary AES circuit to defend against power attacks, which can balance the power consumption when processing different data. Besides, to defend against fault attacks, in the dual complementary AES circuit, we design an improved random infection mechanism to diffuse the effect of injected faults. Experiment results show that the proposed countermeasure can thwart both power and fault attacks effectively. Compared with those AES circuits which can only defend against single attack, our designed circuit increases greatly the security under extra 83.1% area overhead and 2.1% impacts on the maximum working frequency.

Research on a Model of Extracting Persons` Information Based on Statistic Method and Conceptual Knowledge

( Xiang Feng Wei ),( Ning Jia ),( Quan Zhang ),( Han Fen Zang ) 한국언어정보학회 2007 학술대회 논문집 Vol.2007 No.-

In order to extract some important information of a person from text, an extracting model was proposed. The person``s name is recognized based on the maximal entropy statistic model and the training corpus. The sentences surrounding the person``s name are analyzed according to the conceptual knowledge base. The three main elements of events, domain, situation and background, are also extracted from the sentences to construct the structure of events about the person.

Efficient adsorption capability of banana and cassava biochar for malachite green: Removal process and mechanism exploration

Hua Deng,Ya Fen Li,Shu Qi Tao,An Yu Li,Qiu Yan Li,Le Ning Hu 대한환경공학회 2022 Environmental Engineering Research Vol.27 No.3

In this study, banana biochar (BB) and cassava biochar (CB) were roasted in a tube furnace at 400℃ for 2 h, and their adsorption effects on malachite green (MG) were studied. The removal of MG with respect to the solution concentration and reaction time was also investigated. The results show that the optimal amount of biochar is 2 g/L. Studies of the adsorption kinetics and adsorption isotherms show that the pseudo-second-order kinetics can most accurately reflect the adsorption process. Freundlich model fits the experimental data well. The theoretical saturated adsorption capacities of BB and CB are 1,092.80 mg/g and 261.42 mg/g, respectively. SEM-EDS, TGA, BET, FTIR, XRD, element analysis and other characterization techniques were used to explore the adsorption mechanism. Based on the characterization results, it is speculated that the adsorption of BB and CB for MG mainly includes chemical adsorption and physical adsorption.

Metformin Inhibits Nuclear Receptor TR4–Mediated Hepatic Stearoyl-CoA Desaturase 1 Gene Expression With Altered Insulin Sensitivity

Kim, Eungseok,Liu, Ning-Chun,Yu, I-Chen,Lin, Hung-Yun,Lee, Yi-Fen,Sparks, Janet D.,Chen, Lu-Min,Chang, Chawnshang American Diabetes Association 2011 Diabetes Vol.60 No.5

<P><B>OBJECTIVE</B></P><P>TR4 is a nuclear receptor without clear pathophysiological roles. We investigated the roles of hepatic TR4 in the regulation of lipogenesis and insulin sensitivity in vivo and in vitro.</P><P><B>RESEARCH DESIGN AND METHODS</B></P><P>TR4 activity and phosphorylation assays were carried out using hepatocytes and various TR4 wild-type and mutant constructs. Liver tissues from TR4 knockout, C57BL/6, and <I>db/db</I> mice were examined to investigate TR4 target gene stearoyl-CoA desaturase (<I>SCD</I>) 1 regulation.</P><P><B>RESULTS</B></P><P>TR4 transactivation is inhibited via phosphorylation by metformin-induced AMP-activated protein kinase (AMPK) at the amino acid serine 351, which results in the suppression of SCD1 gene expression. Additional mechanistic dissection finds TR4-transactivated <I>SCD1</I> promoter activity via direct binding to the TR4-responsive element located at −243 to −255 on the promoter region. The pathophysiological consequences of the metformin→AMPK→TR4→SCD1 pathway are examined via TR4 knockout mice and primary hepatocytes with either knockdown or overexpression of TR4. The results show that the suppression of <I>SCD1</I> via loss of TR4 resulted in reduced fat mass and increased insulin sensitivity with increased β-oxidation and decreased lipogenic gene expression.</P><P><B>CONCLUSIONS</B></P><P>The pathway from metformin→AMPK→TR4→SCD1→insulin sensitivity suggests that TR4 may function as an important modulator to control lipid metabolism, which sheds light on the use of small molecules to modulate TR4 activity as a new alternative approach to battle the metabolic syndrome.</P>

Novel Core Test Wrapper Design Supporting Multi-mode Testing of NoC-based SoC

Zhang Ying,Wu Ning,Ge Fen,Chen Xin,Zhou Lei 보안공학연구지원센터 2013 International Journal of Control and Automation Vol.6 No.5

Sludge deep dewatering enhanced by zero-valent iron/peroxymonosulfate/walnut shell powder

Zhang Yanping,He Miaolin,Xue Xieping,Li Fen,Lv Ning,Dong Jinghao 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.8

To enhance sludge dewatering performance, Zero-valent iron/peroxymonosulfate/walnut shell powder was used to condition sludge at 55 °C and pH=3 (ZVI/PMS/WSP-T/pH), and the dewatering mechanism was also analyzed. The results showed that the capillary suction time (CST) and water content of sludge cake (Wc) were reduced to 6.8 s and 62.1%, respectively, and net sludge yield (YN) increased to 58.74kg/(m2·h) after being treated by ZVI/PMS/WSP-T/pH. The thermal and acid conditions could promote the corrosion of Fe2+ from ZVI and enhance PMS to produce more SO −· 4 and ·OH. The radicals combined with acid and thermal hydrolysis could efficiently reduce extracellular polymeric substances (EPS), especially tightly bound EPS (TB-EPS), destroy sludge floc and release bound water. The stripping off of EPS and neutralization of Fe3+, Fe2+ and H+ caused the zeta potential to increase to −0.91 mV The flocculation of cations and adsorption of WSP further increased the fractal dimension to 1.71. Moreover, the rigid and porosity structure of WSP increased sludge incompressibility and formed channels for water. Under the combined oxidation, acid/thermal hydrolysis, re-aggregation and skeleton builder functions, the sludge dewatering performance was greatly improved.

Characterization and function of a short peptidoglycan recognition protein from the Chinese oak silkworm, Antheraea pernyi

Li-Shang Dai,Cen Qian, LeiWang,Guo-Qing Wei,Qiu-Ning Liu,Yu Sun,Cong-Fen Zhang,Jun Li,Dong-Ran Liu,Bao-Jian Zhu,Chao-Liang Liu 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.4

Peptidoglycan recognition proteins (PGRPs) are conserved proteins in animals from insects to mammals and play an important role in immune response by recognizing peptidoglycan on microbial surfaces. In this study, a PGRP gene from Antheraea pernyi, named Ap-PGRP-A, was isolated and characterized. Sequence analysis revealed that the full-length cDNA of Ap-PGRP-A was 961 bp, containing a 40 bp 5′-untranslated sequence, a 339 bp 3′-untranslated region and an open reading frame of 582 bp. This gene encoded a putative protein of 193 amino acid residues. Phylogenetic analysis indicated that Ap-PGRP-A had the closest protein sequence similarity to Antheraea mylitta PGRP. The recombinant protein was successfully expressed in Escherichia coli cells and a rabbit anti-Ap-PGRP-A antibody was also prepared. Real-time quantitative reverse transcription-PCR analysis showed that Ap-PGRP-A was extensively expressed in the fat body, midgut, hemocyte,malpighian tubule and epidermis of A. pernyi, especially in the fat body and midgut. The expression levels of Ap-PGRP-A were significantly up-regulated by three types of microorganisms, and Ap-PGRP-A expression was more sensitive in response to the Gram-negative bacterium E. coli than the Gram-positive bacterium Bacillus subtilis or the fungus Beauveria bassiana. These data indicate that Ap-PGRP-A may play a role in the innate immune responses of A. pernyi.