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Survey of the Fresh Rice Straw Silage in Mid-Western Costal Area in Korea
Eui Soo Chung,Jong Geun Kim,Meing Joong Kim,Sung Seo 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08
Recently, round bale silage is very popular preservation method in Korea. In autumn, many farmers usually make silage from fresh rice straw, but the quality of rice straw silage was not good. This study was conducted to find out the silage quality of fresh rice straw in the mid-western costal area in Korea. The average DM (dry matter) content was 40.5% and pH was 5.0. Forage quality of "A" and "D" farm silage was higher than that of others area. Silage quality was also higher in "A" and "D" farm silage of west costal area. The content of lactic acid of "A" and "D" farm silage was high and acetic and butyric acid was low. The result of this experiment indicated that the quality of fresh rice straw silage made by farmers was somewhat low. So we must guide farmers to making good quality of fresh rice straw silage.
Chung, Eui-Ryong,Shin, Sung-Chul,Heo, Jae-Pil Korean Society for Food Science of Animal Resource 2011 한국축산식품학회지 Vol.31 No.4
It is well established that uncoupling protein 3 (UCP3) is expressed largely in skeletal muscle, white adipose tissue and brown adipose tissue and has been suggested to play important roles in regulating energy expenditure, body weight, thermoregulation as well as fatty acid metabolism and obesity. Therefore, the UCP3 gene was selected as a candidate gene for carcass and meat quality traits in Korean cattle. The objective of this study was to identify single nucleotide polymorphisms (SNPs) in the UCP3 gene and to evaluate the association of UCP3 SNP markers with carcass and meat quality traits in Korean cattle. The five exons in the UCP3 gene were sequenced, and ten SNPs were identified. The PCR-SSCP method was then developed to genotype the individuals examined. The g.3076A>G genotype was significantly associated with marbling score (MS) of Korean cattle. Animals with the AA genotype had a higher MS than those with the AG and GG genotypes. No significant associations of the SNP g.3076A>G were observed for any traits. In conclusion, although SNP g.3076A>G, which showed an association with MS, does not cause amino acid changes, this SNP may be used as a DNA marker to select animals that have higher intramuscular fat content.
Expression of Neurotrophic Factors and their Receptors in Keratoconic Cornea
Chung, Eui Sang,Lee, Kwang Hyun,Kim, Min,Chang, Eun Ju,Chung, Tae Young,Kim, Eung Kweon,Lee, Hyung Keun Informa Healthcare USA, Inc. 2013 Current eye research Vol.38 No.7
<P><I>Purpose</I>: To measure the expression level and pattern of neurotrophic factors and their receptors in keratoconus (KC) cornea using quantitative RT-PCR (qPCR) and immunostaining.</P><P><I>Materials and Methods</I>: Twenty-one recipient corneal buttons after keratoplasty from KC cornea and four age-matched normal corneas were obtained. The 25 corneal tissues were divided into two pieces; one fragment of each sample was used for immunostaining whereas the other fragment was used for qPCR. Using primary antibodies and specific qPCR primer, immunostaining and qPCR were performed to analyze the expression level of the neurotrophic factors and receptors.</P><P><I>Results</I>: Nerve growth factor and its receptors (TrkA and p75NTR) were not expressed in the center of normal cornea. However, TrkA and the p75NTR were clearly expressed in a membrane bound staining pattern and the mRNA levels were significantly higher in KC (<I>p</I> < 0.001). The mRNA expression of brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF) and neurotrophin (NT)-4 mRNA was also elevated in KC (<I>p</I> < 0.001). BDNF was expressed in epithelium of normal cornea. However, in KC, its expression was found to be extending into the anterior stromal layer. CNTF was hardly expressed in normal cornea. In KC, the entire epithelium expressed CNTF, especially in perinuclear area. NT-4 was expressed throughout the epithelium and stroma in KC.</P><P><I>Conclusion</I>: The change in expression of neurotrophic factors in KC may suggest that these factors play an important role in the pathogenesis of KC and serve as new markers for the progression of KC.</P>
Chung, Woo Jin,Simmonds, Adam G.,Griebel, Jared J.,Kim, Eui Tae,Suh, Hyo Seon,Shim, In‐,Bo,Glass, Richard S.,Loy, Douglas A.,Theato, Patrick,Sung, Yung‐,Eun,Char, Kookheon,Pyun, Jeffrey WILEY‐VCH Verlag 2011 Angewandte Chemie Vol.123 No.48
<P><B><I>Der Einsatz von elementarem Schwefel</I></B> als Reaktionsmedium für die Herstellung von Goldnanopartikeln und vulkanisierten Nanokompositen wird in der Zuschrift von J. Pyun et al. auf Seite 11 611 ff. beschrieben. Flüssiger Schwefel dient in diesem System als Lösungsmittel, Reduktionsmittel und Stabilisator für die Herstellung von Goldkolloiden. Potenziell nutzbarer elementarer Schwefel fällt in riesigen Mengen beim Raffinieren von Erdöl an.</P>
Eui-Cheol Shin,Chung Eun Hwang,Byong Won Lee,Hyun Tae Kim,Jong Min Ko,In Youl Baek,Yang-Bong Lee,Jin Sang Choi,Eun Ju Cho,Weon Taek Seo,Kye Man Cho 한국식품영양과학회 2012 Preventive Nutrition and Food Science Vol.17 No.3
The purpose of this study was to investigate the fatty acid profiles in 18 soybean cultivars grown in Korea. A total of eleven fatty acids were identified in the sample set, which was comprised of myristic (C14:0), palmitic (C16:0), palmitoleic (C16:1, ?7), stearic (C18:0), oleic (C18:1, ?9), linoleic (C18:2, ?6), linolenic (C18:3, ?3), arachidic (C20:0), gondoic (C20:1, ?9), behenic (C22:0), and lignoceric (C24:0) acids by gas-liquid chromatography with flame ionization detector (GC-FID). Based on their color, yellow-, black-, brown-, and green-colored cultivars were denoted. Correlation coefficients (r) between the nine major fatty acids identified (two trace fatty acids, myristic and palmitoleic, were not included in the study) were generated and revealed an inverse association between oleic and linoleic acids (r=-0.94, p<0.05), while stearic acid was positively correlated to arachidic acid (r=0.72, p<0.05). Principal component analysis (PCA) of the fatty acid data yielded four significant principal components (PCs; i.e., eigenvalues>1), which together account for 81.49% of the total variance in the data set; with PC1 contributing 28.16% of the total. Eigen analysis of the correlation matrix loadings of the four significant PCs revealed that PC1 was mainly contributed to by oleic, linoleic, and gondoic acids, PC2 by stearic, linolenic and arachidic acids, PC3 by behenic and lignoceric acids, and PC4 by palmitic acid. The score plots generated between PC1-PC2 and PC3-PC4 segregated soybean cultivars based on fatty acid composition.
Eui-Jong Chung,김태엽,Jong Min Hwang,Sang-Phil Kim,양현숙 한국심초음파학회 2012 Journal of Cardiovascular Imaging (J Cardiovasc Im Vol.20 No.1
Hemolytic anemia is recognized as a rare complication of mitral valve replacement or repair. We report on a 44-year-old man with shortness of breath and hemolytic anemia, 23 years after mitral valve replacement (Hall-Kaster), and a 63-year-old woman diagnosed of hemolytic anemia, 4 years after mitral and tricuspid annuloplasty (Tailor ring, An-core ring). Routine 2-dimensional transthoracic echocardiography revealed paravalvular leakage around the prosthesis. Subsequent real-time 3-dimensional (3D)transesophageal echocardiography helped the perceptional appreciation of the leakage and the measuring of the regurgitant orifice area using the anatomically correct plane. Surgical findings of each case fit those of 3D volumetric images.
Elemental Sulfur as a Reactive Medium for Gold Nanoparticles and Nanocomposite Materials
Chung, Woo Jin,Simmonds, Adam G.,Griebel, Jared J.,Kim, Eui Tae,Suh, Hyo Seon,Shim, In‐,Bo,Glass, Richard S.,Loy, Douglas A.,Theato, Patrick,Sung, Yung‐,Eun,Char, Kookheon,Pyun, Jeffrey WILEY‐VCH Verlag 2011 Angewandte Chemie Vol.123 No.48
<P><B>Schwefelgelb:</B> Elementarer Schwefel wird als unkonventionelles Medium für die Synthese und Stabilisierung von kolloidalem Gold genutzt. In diesem System erfüllt Schwefel viele Funktionen, wie die Sulfurierung von PPh<SUB>3</SUB> und die Solubilisierung und Reduktion von Au<SUP>I</SUP>‐Vorstufen zu Au‐Nanopartikeln (NPs; siehe Bild). Die Vulkanisation von Au‐haltigen Schwefeldispersionen erfordert vernetzte Nanokomposite, die mit TEM, XRD, XPS und Raman‐Spektroskopie nachgewiesen wurden.</P>
Eui Suk Chung,임청,이해영,Jin-Ho Choi,Jeong-Sang Lee,Kay-Hyun Park 대한흉부외과학회 2011 Journal of Chest Surgery (J Chest Surg) Vol.44 No.4
Background: Despite aggressive treatment, the mortality rate of cardiogenic shock with acute myocardial infarction (AMI) is high. We performed extracorporeal membrane oxygenation (ECMO) prior to coronary reperfusion, and evaluated the early clinical results and risk factors. Materials and Methods: From May 2006 to November 2009, we reviewed the medical records of 20 patients in cardiogenic shock with AMI (mean age 67.7±11.7 yrs, M : F 14 : 6). After initially performing ECMO using the CAPIOX emergency bypass system (EBS^ⓇTerumo, Tokyo, Japan), patients underwent coronary reperfusion (coronary artery bypass grafting, 13; percutaneous coronary intervention, 7). Results: All patients were in a cardiogenic shock state, cardiopulmonary resuscitations (CPR) were performed for fourteen patients (mean CPR time 20.8±26.0 min). The mean time from vascular access to the initiation of ECMO was 17.2±9.4 min and mean support time was 3.8±4.0 days. Fourteen patients were able to be weaned from ECMO and ten patients were discharged (mean admission duration 50.1±31.6 days). Patients survived on average 476.6±374.6 days of follow-up. Longer CPR and support time, increased cardiac enzyme, lower ejection fraction, lower albumin, and major complications were the risk factors of mortality (p<0.05). Conclusion: The early application of ECMO prior to coronary reperfusion and control of risk factors allowed for good clinical results in cardiogenic shock with AMI.
Chung, Eui-Ryong,Shin, Sung-Chul,Heo, Jae-Pil Korean Society for Food Science of Animal Resource 2011 한국축산식품학회지 Vol.31 No.4
Biological or physiological genes that regulate metabolism and energy partitioning have the potential to influence economically important traits such as carcass and meat quality traits in beef cattle. The neuropeptide Y (NPY) functions as a central appetite stimulator and plays a major role in feed intake and energy-balance control. Therefore, the NPY gene is an excellent biological and physiological candidate gene for body weight, feeding, fatness or growth related traits in beef cattle. The objective of this study was to identify single nucleotide polymorphisms (SNPs) in the NPY gene and to evaluate the association of NPY SNP markers with carcass and meat quality traits in Korean cattle. The genomic region (711 bp) including intron 2 of NPY gene was amplified and sequenced, and five SNPs, g.4389 Del(C), g.4371Del(C), g.4271T>C, g.1899A>G and g.1517A>C, were identified. The PCR-RFLP method was then developed to genotype the individuals examined. The g.4271T>C SNP was significantly associated with M. Longissimus dori area (LDA) value (p<0.027). Animals with the TT ($78.144{\pm}0.950\;cm^2$) genotype had higher LDA than those with the CC ($72.266{\pm}2.039\;cm^2$), and animals with TC genotype showed intermediate value. This SNP genotype also showed a highly significant additive genetic effect for the LDA (p<0.01). No significant associations, however, was detected between any of the SNP genotype and other carcass traits measured in this study. In conclusion, SNP genotype of the NPY gene may be used as DNA markers to select animals that have a higher meat yield.