야생동충하초로부터 우량균주선발(II)

방극소(Keuk-So Pang),홍경환(Kyung-Hwan Hong),조덕현(Duck-Hyun Cho) 한국자원식물학회 2001 한국자원식물학회지 Vol.14 No.2

2000년 5월 중순부터 10월까지 채집하였다. 이 중 2속 4종을 확인하였고 6균주를 분리하여 모두를 자실체 유도실험에 이용하였다. 그 결과 동충하초 [Corcyceps miliraris(CHO-7410)] 1균주, 눈꽃동충하초[isaria japoica (CHO-6713, CHO-7413)] 2균주를 상업적이나 의약품개발에 있어서의 시료등 각 산업에있어서 전반적으로 이용가치가 충분히 있다고 사료되어 우량균주로 선발하였다. Many Cordyceps spp. were collected for excellent strains selection at near Wanju-kun from May to October, 2000. They were identified. As the resulting, They were 2 genera and 4 species. 6 strains were got through 4 species culture. 6 strains are Cordyceps militaris f. albino, C, gracilaides, C. militaris and Isaria japoica. Among them, 4 species used for induction of artifitial fruiting body. 3 strains of them were selected for excellent strains. excellent strains are Cordyceps militaris and Isaria japoica.

무기계 항균제(Ag-ZA)의 항균력에 관한 조사

조덕제,정숙현,박동근,신춘환 東西大學校 1996 동서논문집 Vol.2 No.1

Zeolite-Na에 Ag이온을 치환시켜 제조한 무기계 항균제를 이용하여 식품의 부패나 식중독을 초래할 수 있는 5종의 세균에 대한 항균력을 조사하였다. 이 들 5종에 대한 최소 저해 농도는 Escherichia coli는 0.1%이상, Pseudomonas aeruginosa는 0.01%, Klebsiella pneumoniae는 0.05%, Staphylococcus aureus와 Bacillus subtlis는 0.03%로 나타났다. 이 들 세균에 대한 0.1%의 Ag-ZA 첨가에서의 살균효과는 90%의 균 사멸시간으로 나타낼 때 Escherichia coli는 70분, Bacillus subtlis는 92분, Staphylococcus aureus는 111분, Klebsiella pneumoniae는 158분, Pseudomonas aeruginosa는 231분으로 나타났고, Ag-ZA 첨가량의 증가에 따른 사멸 속도의 변화는 Gram 음성균에서는 속도의 증가가 나타나지만 Gram 양성균에서는 속도의 변화를 관찰할 수 없었다. The investigation of antimicrobial activity on the Ag-ZA which is prepared by ion exchanging between Zeolite-Na and Ag ion. Minimum inhibitory concentrations are 0.01% for Escherichia coli and Pseudomonas aeruginosa, 0.05% for Klebsiella pneumoniae and 0.03% for Staphylococcus aureus and Bacillus subtlis. 90% sterilization time with 0.1% Ag-ZA are 70min., 92min., 111min., 158min., 231min. for Escherichia coli, Bacillus subtlis, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa respectively. The sterilization effect is more important in gram neative bacteria than gram positive bacteria.

Notes on the Korean Ascomycetes (IX)

Cho,Duck-Hyun,Lee,Jong-Il 한국자원식물학회 2002 Plant Resources Vol.5 No.2

Many ascomycetes were collected at Mt. Minjuji, Mt. Manduck, Mt. Odae, Mt. Yonsuk and Mt. Sunun from August 1999 to September 2000. They were identified. According to the reusulting, Helvella villosa (Hedw.: O. Kuntze) Dissing and Nannfedt, Dasychyphus corticalis (Pers.:Fr.) Mass., Lachnellula fuscosanguinea(Rhem) Dennis, Lachnellula subtilissima (Cke.) Dennis and Scutellina erinaceus (Schw.) Kunrze are newly to Korea. They were designed Korean common names by authors.

Diversity and Geographic Distribution of Anti-cancer Higher Fungi in Korea

Cho,Duck-Hyun 한국자원식물학회 1998 한국자원식물학회지 Vol.11 No.4

Many higher fungi were collected at Korea from 1976 to 1998. They were identified and surveyed on resources with many reference books. According to the results, fungal fungi were 40 families, 90 general and 215 species. Among them , anti-cancer resources used in Korea were Ganoderma lucidum, Phellinus linteus, Agaricus brazei and Cordyceps militaris. Three species exception Agaricus brazei were distributed in Korea. All these are cultivated in Korea.

Schizophyllum commune의 ribosomal RNA의 生合成에 關한 硏究

趙德炫,吳世源 광주보건대학 1985 論文集 Vol.10 No.-

Biosynthesis and processing of cytoplasmic rRNA precursor during life cycle of Schizophyllum commune Fr. were studied by using ^3H-uridine. 1. Incorporations of ^3H-uridine were high during primordia formation stage in second hyphae and were high during 288 hours cultures inmonohyphae but declined during 240 hours cultures. Incorporations of ^3H-uridine into rRNA were more rapid 2.5-folds at second hyphae than monohyphae. 2. Biosynthesis of RNA were high at primordia formation stage in second hyphae and were high during period cultures from 240 hours to 288 hours at monohyphae. Biosynthesis of RNA were higher 2-folds at second hyphae than monohyphae.

연쇄상구균을 실험감염시킨 넙치치어의 병리조직학적 관찰

조성환,류시윤,김현수,박배근,김덕환,손화영,윤원기,신창호 충남대학교 수의과대학 동물의과학연구소 1999 動物醫科學硏究誌 Vol.7 No.-

Pathological observations were examined after abdominal inoculation in 7∼8㎝ sized flounder (Paralichthys olivaceus) with 1X10^7 cfu/㎖ of Streptococcus faecalis. The infected flounder were clinically observed and weighed a fish body at 0, 5, 10, 15, 20, 25, 30 days post inoculation. In infected group, mortality were 70% and feed efficiency were 7.2%. In non-infected group, mortality were 10% and feed efficiency were 18.2%. Macroscopically, the significant lesions were characterized by protrusions and opacity of the eyes and ulcer of the caudal pecuncle. Histopathologically, the lesions were observed a degeneration. necrosis and inflammatory cells in muscle of the caudal peduncle and a degeneration and necrosis of the rod cells and detached retina from the black pigmented layer

한국산 외대버섯속의 기록(Ⅷ)

조덕현 又石大學校 1993 論文集 Vol.15 No.-

1991년 6월부터 9월까지 지리산국립공원, 내장산국립공원, 선운산도립공원, 그리고 전주근교의 만덕산에서 많은 외대버섯을 채집하여 동정하였다. 그결과 한국산 미기록종으로 확인 된 것은 Entoloma fuliginosum (흑가루외대버섯), E. fumosum (연기외대버섯), E. murrillii (오백색외대버섯), E. parvipapillatum (범젖꼭지외대버섯), E. griseobrunneum (회갈색외대버섯), E. subfloridanum (꽃송이외대버섯), 그리고 E. parvum (돛단외대버섯) 이다. 이 종들에 대하여 분류학적 기재와 한국 표준명을 신칭하였다. Many species of the genus Entoloma were collected at Mt. Jiri National Park, Mt. Naejang National Park, Mt. Sunun Provincial Park and Mt. Manduck from June, 1991 to September, 1991. These species were identified. According to the results, Entoloma fuliginosum, E. fumosum, E. murrillii, E. parvipapillatum, E. griseobrunneum, E. subfloridanum and E. parvum were newly recorded in Korea. These species were described and thier Korean names were designed.

月出山 一帶의 高等菌類

趙德炫 又石大學校 1988 論文集 Vol.10 No.-

日本産 高等菌類

趙德炫 又石大學校 1994 論文集 Vol.16 No.-

1994년 6월 20일부터 1994년 7월 17일까지 일본의 滋賀, 大分縣, 九州의 黑岳 그리고 鹿兒島대학의 다루미즈 연습림에서 일본산 고등균류를 채집하여 동정하였다. 그 결과 2아문, 4강, 8목, 23과, 40속, 50종을 확인하였다. 그 중에서 Tilachlidiopsis nigra와 Isaria atypicola는 아직까지 한국에 보고되지 않은 중이다. Many species of Japanese higher fungi were collected at Shiga-Ken, Kuro-Take, Oita-Ken and the experiment forests of Kagoshima university. These species were identified. According to the results, they are composed of 2 subdivisions, 4 classes, 8 oders, 23 families, 40 genera and 50 species. Among them, Tilachlidiopsis nigra and Isaria atypicola were not reported in Korea.

쑥을 첨가한 두부의 제조 및 저장 중 품질변화

조덕제,정숙현,조만기 東西大學校 1996 동서논문집 Vol.2 No.1

쑥의 효과적인 이용방법을 위해서 쑥의 최적건조 조건을 설정하고, 대두와 숙의 기능적 특성을 잘 살린 쑥을 첨가한 두부의 최적 가공조건과 제품의 저장 중 품질변화에 대해서 실험한 결과를 요약하면 다음과 같다. 1. 건조과정 중 쑥은 조단백질, 조지방 및 조회분은 감소하였으며, 반면 탄수화물은 증가하였다. 2. 1% NaHCO3에 데치기 하여 동결건조한 제품(FDB)이 쑥의 색도유지가 가장 잘 되었으며, Chlorophyll 잔존률도 가장 많았다. 3. 쑥을 첨가한 두부의 제조에서 응고제 GDL, 응고농도 0.1%, 응고온도 70-80℃, 쑥분말 첨가량 0.2%가 최적가공 조건이었다. 4. 쑥을 첨가한 두부의 저장에서 색도변화 및 TBA가의 변화로 미루어 보아 30℃에서는 12시간, 5℃에서는 4일 정도까지 저장 가능한 것으로 판단되었다. An investigation was carried out to study the optimum drying condition of mugwort, the optimum processing conditions of soybean curd added mugwort, and the quality change during storge of soybean curd added mugwort. Crude protein and crude lipid contents of mugwort were decreased during drying process, the contents of those in dried mugwort were 18.0-21.2%, 1.3%-4.0%, respectively. The freeze drying after blanching in 1% NaHCO3 solution(FDB) was the best in color index of dried mugwort, also the highest in retention ratio of total chlorophyll content. The yield of soybean curd with coagulants were showed that MgCl2, CaCl2, CaSO4 and GDL were 3.4g, 3.0g, 3.5g and 4.2g per 30g soy milk, respectively. GDL showed the highest yield among coagulants, and amount of GDL was suited at 0.1%. The optimum coagulation temperature was 70-80℃, addition amount of powered mugwort was 0.2%. The change of color index and TBA value were increased during storage. The quality of soybean curd added mugwort was maintained up to 12 hrs or 4 days at 30℃ or 5℃ storage, respectively.