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Lee, Young Ju,Lee, Doyeon,Shin, So Min,Lee, Jin Sun,Chun, Hyun Soo,Quan, Fu-Shi,Shin, Jae Ho,Lee, Gi-Ja ELSEVIER SCIENCE B.V.; AMSTERDAM 2017 JOURNAL OF FUNCTIONAL FOODS Vol.33 No.-
<P><B>Abstract</B></P> <P>In this study, we investigated the cardioprotective effects of fermented garlic extract (FGE) against ischemia-reperfusion (IR) injury in H9c2 cells and myocardium. FGE used in this study is rich in nitrite (1.42mg/ml of FGE) and reduced glutathione (GSH, 3.23mg/ml of FGE). We compared the effectiveness of FGE against HR–induced oxidative stress with that of nitrite, GSH, and a mixture of nitrite and GSH (nitrite+GSH). In addition, we utilized fluorinated xerogel-derived oxygen microsensors to measure the changes in oxygen level (<I>p</I>O<SUB>2</SUB>) of the myocardium during myocardial IR by FGE. As a result, the <I>p</I>O<SUB>2</SUB> in the FGE group restored to pre-ischemic level (75.1±36.9%) at reperfusion period; however, that of the control group did not increase throughout reperfusion period (2.0±1.7%, <I>p</I> <0.05). Therefore, pre-treatment with FGE might be helpful as a therapeutic strategy for preventing H9c2 cells and myocardium from IR injury.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We investigated the cardioprotective effect of FGE against myocardial IR injury. </LI> <LI> FGE was rich in natural nitrite (1.422mg/ml) and glutathione (3.23mg/ml). </LI> <LI> Pre-treatment with FGE could protect H9c2 cells from HR-induced injury. </LI> <LI> pre-treatment with FGE could promote reoxygenation in post-ischemic myocardiaum. </LI> <LI> FGE might be useful in preventing myocardial IR injury. </LI> </UL> </P>
Yu, Namhee,Yong, Seunghui,Kim, Hong Kwan,Choi, Yoon‐,La,Jung, Yeonjoo,Kim, Doyeon,Seo, Jihae,Lee, Ye Eun,Baek, Daehyun,Lee, Jinseon,Lee, Seungjae,Lee, Jong Eun,Kim, Jaesang,Kim, Jhingook,Lee, Sa John Wiley and Sons Inc. 2019 MOLECULAR ONCOLOGY Vol.13 No.6
<P>The roles of miRNAs in lung cancer have not yet been explored systematically at the genome scale despite their important regulatory functions. Here, we report an integrative analysis of miRNA and mRNA sequencing data for matched tumor–normal samples from 109 Korean female patients with non‐small‐cell lung adenocarcinoma (LUAD). We produced miRNA sequencing (miRNA‐Seq) and RNA‐Seq data for 48 patients and RNA‐Seq data for 61 additional patients. Subsequent differential expression analysis with stringent criteria yielded 44 miRNAs and 2322 genes. Integrative gene set analysis of the differentially expressed miRNAs and genes using miRNA–target information revealed several regulatory processes related to the cell cycle that were targeted by tumor suppressor miRNAs (TSmiR). We performed colony formation assays in A549 and NCI‐H460 cell lines to test the tumor‐suppressive activity of downregulated miRNAs in cancer and identified 7 novel TSmiRs (miR‐144‐5p, miR‐218‐1‐3p, miR‐223‐3p, miR‐27a‐5p, miR‐30a‐3p, miR‐30c‐2‐3p, miR‐338‐5p). Two miRNAs, miR‐30a‐3p and miR‐30c‐2‐3p, showed differential survival characteristics in the Tumor Cancer Genome Atlas (TCGA) LUAD patient cohort indicating their prognostic value. Finally, we identified a network cluster of miRNAs and target genes that could be responsible for cell cycle regulation. Our study not only provides a dataset of miRNA as well as mRNA sequencing from the matched tumor–normal samples, but also reports several novel TSmiRs that could potentially be developed into prognostic biomarkers or therapeutic RNA drugs.</P>
Development of bioinformatics and multi-omics analyses in organoids
Doyeon Ha,JungHo Kong,Donghyo Kim,Kwanghwan Lee,Juhun Lee,Minhyuk Park,Hyunsoo Ahn,Youngchul Oh,Sanguk Kim 생화학분자생물학회 2023 BMB Reports Vol.56 No.1
Pre-clinical models are critical in gaining mechanistic and biologicalinsights into disease progression. Recently, patient-derivedorganoid models have been developed to facilitate our understandingof disease development and to improve the discoveryof therapeutic options by faithfully recapitulating in vivo tissuesor organs. As technological developments of organoid modelsare rapidly growing, computational methods are gaining attentionin organoid researchers to improve the ability to systematicallyanalyze experimental results. In this review, we summarize therecent advances in organoid models to recapitulate human diseasesand computational advancements to analyze experimentalresults from organoids.
The Effects of Preoperative Aspirin on Coronary Artery Bypass Surgery: a Systematic Meta-Analysis
Doyeon Hwang,Joo Myung Lee,Tae-Min Rhee,Young-Chan Kim,Jiesuck Park,Jonghanne Park,Chul Ahn,Young Bin Song,Joo-Yong Hahn,Ki-Bong Kim,Young-Tak Lee,Bon-Kwon Koo 대한심장학회 2019 Korean Circulation Journal Vol.49 No.6
Background and ObjectivesAspirin plays an important role in the maintenance of graft patency and the prevention of thrombotic event after coronary artery bypass graft surgery (CABG). However, the use of preoperative aspirin is still under debate due to the risk of bleeding. MethodsFrom PubMed, EMBASE, and Cochrane Central Register of Controlled Trials, data were extracted by 2 independent reviewers. Meta-analysis using random effect model was performed. ResultsWe performed a systemic meta-analysis of 17 studies (12 randomized controlled studies and 5 non-randomized registries) which compared clinical outcomes of 9,101 patients who underwent CABG with or without preoperative aspirin administration. Preoperative aspirin increased chest tube drainage (weighted mean difference 177.4 mL, 95% confidence interval [CI], 41.3–313.4; p=0.011). However, the risk of re-operation for bleeding was not different between the preoperative aspirin group and the control group (3.2% vs. 2.4%; odds ratio [OR], 1.23; 95% CI, 0.94–1.60; p=0.102). There was no difference in the rates of all-cause mortality (1.6% vs. 1.5%; OR, 0.98; 95% CI, 0.64–1.49; p=0.920) and myocardial infarction (MI) (8.7% vs. 10.4%; OR, 0.83; 95% CI, 0.66–1.04; p=0.102) between patients with and without preoperative aspirin administration. ConclusionsAlthough aspirin increased the amount of chest tube drainage, it was not associated with increased risk of re-operation for bleeding. In addition, the risks of early postoperative all-cause mortality and MI were not reduced by using preoperative aspirin.
Lee, Geumbee,Kim, Daeil,Kim, Doyeon,Oh, Sooyeoun,Yun, Junyeong,Kim, Jihyun,Lee, Sang-Soo,Ha, Jeong Sook The Royal Society of Chemistry 2015 ENERGY AND ENVIRONMENTAL SCIENCE Vol.8 No.6
<P>In this study, we report the fabrication of a stretchable and patchable array of micro-supercapacitors (MSCs) using a gel-type electrolyte of poly(methyl methacrylate)–propylene carbonate–lithium perchlorate. As electrodes, a layer-by-layer-assembled thin film of multi-walled carbon nanotubes with a top layer of Mn<SUB>3</SUB>O<SUB>4</SUB> nanoparticles was used. The fabricated MSC maintained over 85% of its performance for 2 weeks in ambient air without encapsulation owing to the use of a non-aqueous solvent based gel electrolyte. Dry-transferred MSC arrays on a specially designed stretchable polymer substrate exhibited stable electrochemical performance under various deformations, including bending, twisting, both uniaxial and biaxial stretching up to 50%, and winding around the curved substrate. Furthermore, the encapsulated MSC array with a thin polymer film directly attached to skin maintained its electrochemical performance under repeated body movement, cycles of attachment–detachment, and even in water. This study clearly demonstrates a stretchable and patchable MSC array for practical use as an energy storage device that can be attached to the body for electronic function, even under wet conditions.</P> <P>Graphic Abstract</P><P>We report on air stable planar micro-supercapacitors (MSCs) using a non-aqueous solvent based gel electrolyte and stable performance of encapsulated MSC array under stretching and exposure to water. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5ee00670h'> </P>
Evaluating the Comfort Experience of a Head-Mounted Display with the Delphi Methodology
이도연 ( Doyeon Lee ),장병희 ( Byeng-hee Chang ),박지섭 ( Jiseob Park ) 한국인터넷정보학회 2020 인터넷정보학회논문지 Vol.21 No.6
This study developed evaluation indicators for the comfort experience of virtual reality (VR) headsets by classifying, defining, and weighting cybersickness-causing factors using the Delphi research method and analytic hierarchical process (AHP) approach. Four surveys were conducted with 20 experts on VR motion sickness. The expert surveys involved the 1) classification and definition of cybersickness-causing dimensions, classification of sub-factors for each dimension, and selection of evaluation indicators, 2) self-reassessment of the results of each step, 3) validity revaluation, and 4) final weighting calculation. Based on the surveys, the evaluation indicators for the comfort experience of VR headsets were classified into eight sub-factors: field of view (FoV)-device FoV, latency-device latency, framerate-device framerate, V-sync-device V-sync, rig-camera angle view, rig-no-parallax point, resolution-device resolution, and resolution-pixels per inch (PPI). A total of six dimensions and eight sub-factors were identified; sub-factor-based evaluation indicators were also developed.