Mixture of Humulus japonicus, Diospyros lotus, Zingiber officinale and Red Ginseng Extracts Prevents 6-Hydroxydopamine-Induced Cell Damage

Dajeong Shin,Byoungok Cho,Jaeyoung Shin,Jihyeon Park,Feng Wang,Suping Hao,Eunseo Kang,Seonil Jang 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

Oxidative stress in neurons causes DNA breakdown, cell membrane damage, and neuronal cell death, and can cause neurodegenerative changes. Recently, research interest in discovering herbal medicine materials that can protect cells from oxidative stress is increasing. Humulus japonicus, Diospyros lotus, Zingiber officinale and Red Ginseng are oriental medicine materials that have been used in traditional medicine in Korea and China. In the present study we evaluated the ameliorative effects of H. japonicus, D. lotus, Z. officinale and Red Ginseng extracts mixture (HDZR) against 6-OHDA-induced oxidative stress in microglia. As a result, HDZR restored cells damaged by 6-OHDA in microglia. HDZR pretreatment protected the microglia against oxidative stress by reducing cell death, apoptosis and reactive oxygen species generation. HDZR pretreatment also increased superoxide dismutase and catalase enzyme activity. Moreover, HDZR activated the Nrf2/HO-1 pathway. Taken together, these results showed that HDZR treatment has potential as a therapeutic agent against neuron injury through the suppression of oxidative stress.

A Study on the Discovery of IL-31 Receptor A in Astrocytes and Effect of Diospyros lotus Extract on IL-31-Mediated Pruritus in Microglia and Astrocytes

Jaeyoung Shin,Jihyeon Park,Byoungok Cho,Feng Wang,Suping Hao,Dajeong Shin,Youngsoo Kim,Seonil Jang 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

Interleukin-31 is associated with hypersensitivity disorders such as atopic dermatitis, allergic rhinitis and asthma. IL-31 is also known to be one of the major "drivers" of pruritus. In the nervous system, pruritus may be enhanced through IL-31-related interactions. However, there were no reports of IL-31 receptor expression in astrocytes. In this study, the expression of IL-31 receptor in astrocytes and the effect of Diospyros lotus extract (DLE) on pruritus factors between microglia and astrocytes were investigated. As a result, the expression of IL-31 receptor A in astrocytes was discovered for the first time. Pretreatment with DLE suppressed the expression of IL-31 receptor A in IL-31-treated astrocytes. On the other hand, pretreatment of DLE in microglia inhibits lipopolysaccharide (LPS) plus interferon gamma (IFN-γ)-induced IL-31 production. DLE inhibited phosphorylation of MAPK, STAT1 and NF-κB in LPS plus IFN-γ stimulated microglia. Taken together, DLE down-regulated IL-31-mediated pruritus factors in both astrocytes and microglia. Therefore, the results of this study suggest the potential of DLE as a material that can relieve chronic itch conditions.

Health Span-Extending Activity of Human Amniotic Membrane- and Adipose Tissue-Derived Stem Cells in F344 Rats.

Kim, Dajeong,Kyung, Jangbeen,Park, Dongsun,Choi, Ehn-Kyoung,Kim, Kwang Sei,Shin, Kyungha,Lee, Hangyoung,Shin, Il Seob,Kang, Sung Keun,Ra, Jeong Chan,Kim, Yun-Bae unknown 2015 Stem cells translational medicine Vol.4 No.10

<P>: Aging brings about the progressive decline in cognitive function and physical activity, along with losses of stem cell population and function. Although transplantation of muscle-derived stem/progenitor cells extended the health span and life span of progeria mice, such effects in normal animals were not confirmed. Human amniotic membrane-derived mesenchymal stem cells (AMMSCs) or adipose tissue-derived mesenchymal stem cells (ADMSCs) (1 ?? 10(6) cells per rat) were intravenously transplanted to 10-month-old male F344 rats once a month throughout their lives. Transplantation of AMMSCs and ADMSCs improved cognitive and physical functions of naturally aging rats, extending life span by 23.4% and 31.3%, respectively. The stem cell therapy increased the concentration of acetylcholine and recovered neurotrophic factors in the brain and muscles, leading to restoration of microtubule-associated protein 2, cholinergic and dopaminergic nervous systems, microvessels, muscle mass, and antioxidative capacity. The results indicate that repeated transplantation of AMMSCs and ADMSCs elongate both health span and life span, which could be a starting point for antiaging or rejuvenation effects of allogeneic or autologous stem cells with minimum immune rejection.</P>

강원도 홍천군 자운리 고랭지밭의 수질인자간 상관관계 분석

홍다정 ( Dajeong Hong ),유나영 ( Nayeong Yu ),임정하 ( Jungha Lim ),신민환 ( Minhwan Shin ) 한국농공학회 2022 한국농공학회 학술대회초록집 Vol.2022 No.-

밭은 비료의 사용량이 많고, 강우시 토양유실이 발생하여 홍수기 하천과 호소의 탁수 문제를 일으키는 주요 원인이 된다. 특히, 고랭지밭 비율이 높은 강원도의 경우 적은 강우에도 토사유출이 심하기 때문에 밭에서 발생하는 오염물질 발생량과 부하량의 관리가 쉽지 않다. 따라서, 고랭지 밭에서 발생하는 오염물질이 하천에 미치는 수질 특성을 파악하는 것이 중요하다. 그러나, 고랭지밭에서는 다양한 BMPs가 적용되어 BMPs별로 발생하는 수질농도를 분석하기에는 경제적 어려움이 있다. 따라서, 본 연구에서는 고랭지밭 비율이 높은 강원도 지역의 수질 모니터링 결과와 통계분석을 통한 수질 항목간의 상관관계를 이용하여 BMPs별 저감효율 평가시 측정항목의 최소화 방안을 검토하고자 하였다. 연구지점은 강원도 홍천군 자운2리에 위치한 시험포이며, 2019년 6월부터 9월까지 대조구와 비닐멀칭, 지표피복, 양파망, 초생대, 호밀 등 6개 시험포에서 5회의 강우유출수 모니터링 결과를 이용하였다. 모니터링 결과는 pH, EC, 탁도, BOD₅, COD_Mn, SS, T-N, T-P, TOC, 유출량이며, 항목간 Pearson 상관관계를 분석하였다. 분석결과 대조구의 경우 TOC 항목은|0.56|(-0.25 ~ 0.96)로 다른 항목에 비해 상관관계가 가장 높은 것으로 나타났으며, EC 항목은 |0.12|(-0.17 ~ 0.55)로 가장 낮게 나타났다. 비닐멀칭의 경우 SS 항목이|0.65|(-0.67 ~ 0.93)로 다른 항목에 비해 상관관계가 가장 높은 것으로 나타났으며, BOD₅ 항목은|0.13|(-0.061 ~ 0 .67)로 가장 낮은 것으로 나타났다. 지표피복의 경우 탁도 항목이|0.45|(-0.45 ~ 0.72)로 가장 상관관계가 높고, 유출량은|0.16|(-0.075 ~ 0 .64)로 가장 낮은 것으로 나타났다. 양파망의 경우 COD_Mn 항목이|0.57|(-0.6 ~ 0 .89)로 가장 높고, EC 항목이|0.10|(-0.094 ~ 0 .45)로 가장 낮은것으로 나타났다. 초생대의 경우 COD_Mn 항목이|0.42|(-0.36 ~ 0.92)로 상관관계가 가장 높고, EC 항목이|0.13|(-0.099 ~ 0.16)로 가장 낮은 것으로 나타났다. 호밀의 경우 COD_Mn 항목이|0.48|(-0.18 ~ 0.96)로 가장 높고, T-P 항목이|0.16|(-0.18 ~ 0.41)로 가장 낮은 상관관계를 보이는 것으로 분석되었다. 분석결과와 같이 비닐멀칭과 볏짚거적의 경우 SS와 탁도 상관성이 가장 높은 것으로 나타나 탁도를 측정하여 SS로 환산하여 측정하는 방법을 적용할 수 있을 것으로 판단된다. 또한, 양파망과 초생대, 호밀의 경우 COD_Mn 항목과 다른 항목간의 상관성이 가장 높은 것으로 나타나 유사한 특성을 가진 BOD₅, TOC 항목을 대신하여 상관관계를 통한 수질농도 추정에 활용할 수 있을 것으로 판단된다. 그러나, 본 연구에서는 BMPs별 발생하는 수질농도간 상관관계가 다양하게 나타나, 밭 뿐만아니라 다양한 토지이용과 토사유출이 심한 하천에서 발생하는 유출수의 수질농도간 상관관계 분석을 통한 추가 연구가 필요할 것으로 보여진다.

강원도 홍천군 자운리 고랭지밭의 수질인자간 상관관계 분석

홍다정 ( Dajeong Hong ),유나영 ( Nayeong Yu ),임정하 ( Jungha Lim ),신민환 ( Minhwan Shin ) 한국농공학회 2022 한국농공학회 학술대회초록집 Vol.2022 No.-

밭은 비료의 사용량이 많고, 강우시 토양유실이 발생하여 홍수기 하천과 호소의 탁수 문제를 일으키는 주요 원인이 된다. 특히, 고랭지밭 비율이 높은 강원도의 경우 적은 강우에도 토사유출이 심하기 때문에 밭에서 발생하는 오염물질 발생량과 부하량의 관리가 쉽지 않다. 따라서, 고랭지 밭에서 발생하는 오염물질이 하천에 미치는 수질 특성을 파악하는 것이 중요하다. 그러나, 고랭지밭에서는 다양한 BMPs가 적용되어 BMPs별로 발생하는 수질농도를 분석하기에는 경제적 어려움이 있다. 따라서, 본 연구에서는 고랭지밭 비율이 높은 강원도 지역의 수질 모니터링 결과와 통계분석을 통한 수질 항목간의 상관관계를 이용하여 BMPs별 저감효율 평가시 측정항목의 최소화 방안을 검토하고자 하였다. 연구지점은 강원도 홍천군 자운2리에 위치한 시험포이며, 2019년 6월부터 9월까지 대조구와 비닐멀칭, 지표피복, 양파망, 초생대, 호밀 등 6개 시험포에서 5회의 강우유출수 모니터링 결과를 이용하였다. 모니터링 결과는 pH, EC, 탁도, BOD₅, COD_Mn, SS, T-N, T-P, TOC, 유출량이며, 항목간 Pearson 상관관계를 분석하였다. 분석결과 대조구의 경우 TOC 항목은|0.56|(-0.25 ~ 0.96)로 다른 항목에 비해 상관관계가 가장 높은 것으로 나타났으며, EC 항목은 |0.12|(-0.17 ~ 0.55)로 가장 낮게 나타났다. 비닐멀칭의 경우 SS 항목이|0.65|(-0.67 ~ 0.93)로 다른 항목에 비해 상관관계가 가장 높은 것으로 나타났으며, BOD₅ 항목은|0.13|(-0.061 ~ 0 .67)로 가장 낮은 것으로 나타났다. 지표피복의 경우 탁도 항목이|0.45|(-0.45 ~ 0.72)로 가장 상관관계가 높고, 유출량은|0.16|(-0.075 ~ 0 .64)로 가장 낮은 것으로 나타났다. 양파망의 경우 COD_Mn 항목이|0.57|(-0.6 ~ 0 .89)로 가장 높고, EC 항목이|0.10|(-0.094 ~ 0 .45)로 가장 낮은것으로 나타났다. 초생대의 경우 COD_Mn 항목이|0.42|(-0.36 ~ 0.92)로 상관관계가 가장 높고, EC 항목이|0.13|(-0.099 ~ 0.16)로 가장 낮은 것으로 나타났다. 호밀의 경우 COD_Mn 항목이|0.48|(-0.18 ~ 0.96)로 가장 높고, T-P 항목이|0.16|(-0.18 ~ 0.41)로 가장 낮은 상관관계를 보이는 것으로 분석되었다. 분석결과와 같이 비닐멀칭과 볏짚거적의 경우 SS와 탁도 상관성이 가장 높은 것으로 나타나 탁도를 측정하여 SS로 환산하여 측정하는 방법을 적용할 수 있을 것으로 판단된다. 또한, 양파망과 초생대, 호밀의 경우 COD_Mn 항목과 다른 항목간의 상관성이 가장 높은 것으로 나타나 유사한 특성을 가진 BOD₅, TOC 항목을 대신하여 상관관계를 통한 수질농도 추정에 활용할 수 있을 것으로 판단된다. 그러나, 본 연구에서는 BMPs별 발생하는 수질농도간 상관관계가 다양하게 나타나, 밭 뿐만아니라 다양한 토지이용과 토사유출이 심한 하천에서 발생하는 유출수의 수질농도간 상관관계 분석을 통한 추가 연구가 필요할 것으로 보여진다.

Polysaccharide isolated from Aloe vera gel suppresses ovalbumin-induced food allergy through inhibition of Th2 immunity in mice

Lee, Dajeong,Kim, Hyuk Soon,Shin, Eunju,Do, Seon-Gil,Lee, Chong-Kil,Kim, Young Mi,Lee, Min Bum,Min, Keun Young,Koo, Jimo,Kim, Su Jeong,Nam, Seung Taek,Kim, Hyun Woo,Park, Young Hwan,Choi, Wahn Soo Elsevier 2018 Biomedicine & pharmacotherapy Vol.101 No.-

<P><B>Abstract</B></P> <P>An allergic reaction occurs when the immune system overreacts to harmless substance called allergen that gains access to the body. Food allergy is a hypersensitive immune reaction to food proteins and the number of patients with food allergy has recently increased. <I>Aloe Vera</I> is used for wellness and medicinal purposes. In particular, <I>Aloe vera</I> has been reported to enhance immunity. However, the effect of <I>Aloe vera</I> on food allergy is not yet known. In this study, we investigated the effects of processed <I>Aloe vera</I> gel (PAG) containing low molecular weight Aloe polysaccharide (AP) on ovalbumin (OVA)-induced food allergy in mice. Allergic symptoms, rectal temperature, and diarrhea were measured in OVA-induced food allergy mice. Other allergic parameters were also analyzed by RT-PCR, ELISA, flow cytometry, and other biochemical methods. As the results, PAG suppressed the decrease of body temperature, diarrhea, and allergic symptoms in OVA-induced food allergy mice. PAG also reduced serum concentrations of type 2 helper T cell (Th2) cytokines (Interleukin-(IL)-4, IL-5, and IL-13) as well as histamine, mast cell protease-1 (MCP-1), and immunoglobulin (Ig)E. PAG blocked the degranulation of mast cells and infiltration of eosinophils in intestine. Furthermore, PAG suppressed the population of Th2 cells in spleen and mesenteric lymph nodes. PAG also increased the production of IL-10 and population of type 1 regulatory T (Tr1) cells in mice with food allergy. Taken together, our findings suggest that PAG suppressed Th2 immune responses through, at least partially, stimulating the secretion of IL-10 in food allergy mice.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Processed <I>Aloe vera</I> gel (PAG) suppresses food allergy in mice. </LI> <LI> PAG inhibits Th2 immune responses in mice. </LI> <LI> PAG increases the production of IL-10 in vitro and in vivo. </LI> <LI> PAG stimulates the activation of Tr1 cells. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Heat Shock Protein B1 and Its Regulator Genes Are Negatively Correlated with Intramuscular Fat Content in the Longissimus Thoracis Muscle of Hanwoo (Korean Cattle) Steers

Kim, Nam-Kuk,Lim, Dajeong,Lee, Seung-Hwan,Cho, Yong-Min,Park, Eung-Woo,Lee, Chang-Soo,Shin, Bong-Shig,Kim, Tae-Hun,Yoon, Duhak American Chemical Society 2011 Journal of agricultural and food chemistry Vol.59 No.10

<P>In previous proteomic studies, heat shock protein β 1 (HSPB1) was detected as a candidate protein related to meat quality in cattle. This study sought to determine if its gene expression was associated with intramuscular fat content in the longissimus thoracis muscle of Korean cattle (Hanwoo). Tissue from two groups of 10 steers each, low-marbling (mean intramuscular fat content, 7.4 ± 1.5%) and high-marbling (23.5 ± 2.8%), were used for immunoblotting, real-time PCR, and statistical analyses. HSPB1 expression in both mRNA and protein was shown to be negatively related to intramuscular fat content (<I>P</I> < 0.05). Pathway analysis found two genes, TNF receptor superfamily member 6 (FAS) and angiotensinogen (AGT), that were regulators of the HSPB1 gene. The expression of the two genes showed a negative correlation with intramuscular fat content (<I>P</I> < 0.05). These results suggest that HSPB1, FAS, and AGT may be good candidate genes associated with intramuscular fat content in the longissimus muscle of Korean cattle.</P>

Rapha diet<SUP>Ⓡ</SUP>, a Combinational Preparation for the Improvement of Dietary Obesity

Jihyun Kim,Jangbeen Kyung,Dajeong Kim,Jingmei Cai,Ehn-Kyoung Choi,Ja Young Jang,Youngeun Kim,Kwang Sei Kim,Kyungha Shin,Dongsun Park,Yun-Bae Kim 한국실험동물학회 2013 한국실험동물학회 학술발표대회 논문집 Vol.2013 No.2

Artificial light-harvesting n-type porphyrin for panchromatic organic photovoltaic devices

Hadmojo, Wisnu Tantyo,Yim, Dajeong,Aqoma, Havid,Ryu, Du Yeol,Shin, Tae Joo,Kim, Hyun Woo,Hwang, Eojin,Jang, Woo-Dong,Jung, In Hwan,Jang, Sung-Yeon Royal Society of Chemistry 2017 Chemical Science Vol.8 No.7

<▼1><P>We developed a novel NIR-harvesting n-type porphyrin derivative, PDI–P<SUB>Zn</SUB>–PDI, that shows a low bandgap of 1.27 eV. Panchromatic absorption was extended to the NIR area with a significantly low energy loss of 0.54 eV which led to promising photovoltaic performance.</P></▼1><▼2><P>A near-infrared-harvesting n-type porphyrin-based acceptor for organic photovoltaics (OPVs) was developed. The n-type acceptor, PDI–P<SUB>Zn</SUB>–PDI, was designed by connecting a zinc porphyrin (P<SUB>Zn</SUB>) core to two perylenediimide (PDI) wings through ethyne bridges. A narrow bandgap of 1.27 eV was achieved through the extended π-conjugation and intramolecular charge transfer between the strongly electron-donating P<SUB>Zn</SUB> core and the electron-accepting PDI wings. A bulk heterojunction (BHJ) structured photovoltaic device fabricated from PDI–P<SUB>Zn</SUB>–PDI with PTB7-Th exhibited panchromatic photon-to-current conversion from 350 to 900 nm. A power conversion efficiency of 5.25% with a remarkably low <I>E</I><SUB>loss</SUB> of 0.54 eV was achieved by optimizing the nanomorphology of the BHJ films by adding pyridine and by controlling the ZnO/BHJ interfacial properties.</P></▼2>

Full-Length Enriched cDNA Library Construction from Tissues Related to Energy Metabolism in Pigs

이경태,Mi-Jeong Byun,Dajeong Lim,Kyung-Soo Kang,김남순,오정화,정정수,Hae-Suk Park,Younhee Shin,김태헌 한국분자세포생물학회 2009 Molecules and cells Vol.28 No.6

Genome sequencing of the pig is being accelerated because of its importance as an evolutionary and biomedical model animal as well as a major livestock animal. However, information on expressed porcine genes is insufficient to allow annotation and use of the genomic information. A series of expressed sequence tags of 5′ ends of five fulllength enriched cDNA libraries (SUSFLECKs) were functionally characterized. SUSFLECKs were constructed from porcine abdominal fat, induced fat cells, loin muscle, liver, and pituitary gland, and were composed of non-normalized and normalized libraries. A total of 55,658 ESTs that were sequenced once from the 5′ ends of clones were produced and assembled into 17,684 unique sequences with 7,736 contigs and 9,948 singletons. In Gene Ontology analysis, two significant biological process leaf nodes were found: gluconeogenesis and translation elongation. In functional domain analysis based on the Pfam database, the beta transducin repeat domain of WD40 protein was the most frequently occurring domain. Twelve genes, including SLC25A6, EEF1G, EEF1A1, COX1, ACTA1, SLA, and ANXA2, were significantly more abundant in fat tissues than in loin muscle, liver, and pituitary gland in the SUSFLECKs. These characteristics of SUSFLECKs determined by EST analysis can provide important insight to discover the functional pathways in gene networks and to expand our understanding of energy metabolism in the pig.