백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.

염농도에 따른 멸치젓의 품질 변화

임상빈,오창경,송대진 제주대학교 산업기술연구소 2000 尖端技術硏究所論文集 Vol.11 No.1

General composition, pH, salinity, peroxide value, TBA value, volatile basic nitrogen(VBN) and amino nitrogen were measured for fermented anchovy with different salt content. Moisture, crude protein, crude lipid and ash of raw anchovy were 75.9, 18.2, 2.36 and 3.9%, respectively. Moisture of fermented anchovy decreased as the increase of salt added. Crude protein and crude lipid were the highest in 12% salt fermented anchovy. Ash of fermented anchovy increased as the increase of salt content. pH and salinity of raw anchovy were 7.0 and 0.61%, respectively. pH of fermented anchovy were 6.0 regardless of salt concentration. Peroxide values increased drastically with the increase of salt added. TBA was the lowest and amino nitrogen was the highest in 8% salt fermented anchovy.

초피 추출물의 수율 및 항균활성

임상빈,좌미경,송대진,고정삼 濟州大學校 産業技術硏究所 1995 산업기술연구소논문집 Vol.6 No.-

Extraction yields of Zunthoxylum piperiturn by organic solvents and supercritical carbon dioxide(SC-CO_(2)) with and without ethanol as a cosolvent, and antimicrobial activities of the extracts against Hucillus cereus were determined. Extraction yield by distilled water was the highest, 20%, while those by ethanol, ethyl ether and hexane were less than 3%. Extraction yield by SC-CO_(2) Lvith ethanol was 1.42 times greater than that by only SC-CO_(2). Ethyl ether- and ethanol-extracts had potent antimicrobial activities against B. cereus. Extraction yield by SC-CO_(2) with ethanol showed almost the same degree of microbial growth inhibition as that by only SC-CO_(2).

Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

초임계 이산화탄소에 의한 멸치어유의 추출

임상빈,좌미경,송대진 濟州大學校 産業技術硏究所 1998 산업기술연구소논문집 Vol.9 No.1

Anchovy oil was extracted by supercritical CO_(2). and extraction yield and fatty acid composition were measured in the extract and residual fractions. EPA and DHA were 7.6~13.3% and 16.2~18.3% for the original anchovy oil. The extraction yield of anchovy oil increased as the extraction temperature decreased. Essentially no great differences were noticed in the fatty acid composition of the oil extracted under different extraction temperatures. Anchovy oil mixed with silver nitrate-coated silica gel was extracted by only SC-CO_(2)for 2 hr and by ethyl acetate-modified SC-CO_(2)for 2 hr at 60℃/276 bar. This produced higher extraction yield and higher concentration of EPA and DHA in the residual fraction. Starting with anchovy oil containing 13.3% EPA and 16.2% DHA. the residue fraction containing 23.9% EPA and 31.1% DHA was obtained.

초임계 이산화탄소에 의한 멸치어유의 추출

임상빈,좌미경,송대진 濟州大學校 工科大學 産業技術硏究所 1998 尖端技術硏究所論文集 Vol.9 No.1

고품질의 고도불포화지방산 제품을 제조할 목적으로, 멸치어유를 초임계 이산화탄소로 추출분획한 후 각 분획들의 추출수율과 지방산 조성을 측정하였다. 멸치어유의 주요 지방산은 palmitic acid. DHA, EPA, palmitoleic acid. myristic acid. oleic acid 였다. 멸치어유의 추출수율은 동일압력에서 추출시간의 증가에 따라 감소하였으며, 40과 50℃에서는 추출온도의 증가에 따라 감소하였으나, 60℃에서는 50℃ 보다 다소 높았다. 동일온도에서 C14와 C16 지방산들은 추출물 분획에 농축되었고, 나머지 지방산들은 추출 잔류물 분획에 농축되었다. EPA는 분획들간에 거의 일정한 경향을 보였으나, DHA는 추출잔류물 분획에 농축되었다. Silver nitrate를 silica gel에 코팅시킨 후 어유와 혼합하여 추출 2시간 동안은 초임계 이산화탄소로만으로 추출하고, 그 후 보조용매로 ethyl acetate를 첨가하여 2시간 동안 추출하였을 때 추출물 분획에서의 저급지방산인 C14:0은 추출잔류물에 비하여 5.0∼6.4배 농축되었고, C16:0은 1.9∼2.0배 농축된 반면, 추출잔류물 분획에서 EPA와 DHA는 23.9와 31.6%로 원료어유의 13.3과 16.2%에 비하여 1.8배와 1.9배 농축되었다. Anchovy oil was extracted by supercritical CO₂, and extraction yield and fatty acid composition were measured in the extract and residual fraction. EPA and DHA were 7.6∼13.3% and 16.2∼18.3% for the original anchovy oil. The extraction yield of anchovy oil increased as the extraction temperature decreased. Essentially no great differences were noticed in the fatty acid composition of the oil extracted under different extraction temperatures. Anchovy oil mixed with silver nitrate-coated silica gel was extracted by only SC-CO₂ for 2 hr and by ethyl acetate-modified SC-CO₂ for 2 hr at 60℃/276 bar. This produced higher extraction yield and higher concentration of EPA and DHA in the residual fraction. Starting with anchovy oil containing 13.3% EPA and 16.2% DHA, the residue fraction containing 23.9% EPA and 31.1% DHA was obtained.

태양열 건조 시스템에 관한 실험적 연구(Ⅱ) - 벼의 태양열 건조 특성 -

고학균(Koh Hak-Kyun),김용현(Kim Yong-Hyeon),송대빈(Song Dae-Bin),박재복(Park Jae-Bok) 한국태양에너지학회 1991 한국태양에너지학회 논문집 Vol.11 No.3

곡물 bin을 이용한 벼의 건조실험을 수행하여 상온통풍건조 시스템과 태양열 건조 시스템의 건조 특성을 비교하여 분석하였다. 태양열 건조의 시뮬레이션 모형에 대한 컴퓨터 프로그램을 개발하였으며 시뮬레이션 모형에 의한 함수율의 예측치는 실측치와 전체적으로 일치하였다.<br/> 또한, inverter를 사용하여 벼의 건조 상태에 따라 송풍량을 적절하게 제어하는 송풍량 제어 시스템을 개발하였으며, 개발된 송풍량 제어 시스템을 통하여 에너지 절약 효과를 얻을 수 있었다. In-bin grain drying experiments were performed to investigate the drying characteristics between natural air and solar heated-air drying system of rough rice. A computer simulation model for solar drying system of rough rice resulted in a good agreement between the experimental and predicted moisture content.<br/> In order to save the electric energy consumption of fan, airflow rates control system using inverter was developed and resulted in the effect of energy saving.

염농도에 따른 멸치젖의 품질 변화

임상빈,오창경,송대진 濟州大學校 産業技術硏究所 2000 산업기술연구소논문집 Vol.11 No.1

General composition, pH. salinity. peroxide value. TBA value. volatile basic nitrogen(VBN) and amino nitrogen were measured for fermented anchovy with different salt content. Moisture. crude protein, crude lipid and ash of raw anchovy were 75.9, 18.2, 2.36 and 3.9%. respectively. Moisture of fermented anchovy decreased as the increase of salt added. Crude protein and crude lipid were the highest in 12% salt fermented anchovy. Ash of fermented anchovy increased as the increase of salt content. pH and salinity of raw anchovy were 7.0 and 0.61%. respectively. pH of fermented anchovy were 6.0 regardless of salt concentration. Peroxide values increased drastically with the increase of salt added. TBA was the lowest and amino nitrogen was the highest in 8% salt fermented anchovy.

초피 추출물의 수율 및 항균활성

임상빈,송대진,좌미경,고정삼 濟州大學校工科大學産業技術硏究所 1995 尖端技術硏究所論文集 Vol.6 No.-

초피를 유기용매 및 초임계이산화탄소와 보조용매로서 에탄올로 추출하여 각각의 추출수율을 측정하고, 이들 추출물들의 항균활성을 검색하였다. 유기용매별로 추출하여 각각의 추출수율을 측정한 결과, 증류수인 경우 20%로서 가장 높았고, 나머지 헥산, 에테르, 에탄올인 경우 3%이하로 매우 적었다. 또한 추출 용매로 초임계이산화탄소-에탄올을 병용했을 때 초피의 추출수율과 용해도는 초임계이산화탄소만을 사용했을 때보다 1.4배 이상 높았다. 용매별 추출물들의 항균성을 검색한 결과 모든 추출물들이 B.cereus에 대해 항균성을 나타내었는데, 특히 에탄올, 에테르 추출물들의 항균성이 더 큰 것으로 나타났다. 초임계이산화탄소-에탄올 추출물은 초임계이산화탄소만을 사용한 추출물과 거의 동일한 항균성을 나타내었다. Extraction yields of Zanthoxylum piperitum by organic solvents and supercritical carbon dioxide(SC-CO₂) with and without ethanol as a cosolvent, and antimicrobial activities of the extracts against Bacillus cereus were determined. Extraction yield by distilled water was the highest, 20%, while those by ethanol, ethyl ether and hexane were less than 3%. Extraction yield by SC-CO₂ with ethanol was 1.42 times greater than that by only SC-CO₂ Ethyl ether- and ethanol-extracts had potent antimicrobial activities against B.cereus. Extraction yield by SC-CO₂ with ethanol showed almost the same degree of microbial growth inhibition as that by only SC-CO₂.

캡슐소재에 따른 멸치어유의 미세캡슐화

임상빈,좌미경,송대진 濟州大學校 産業技術硏究所 1999 산업기술연구소논문집 Vol.10 No.1

Microencapsulation of anchovy oil with different wall materials such as sodium alginate. chitosan and β-cyclodextrin was investigated. Microencapsulation was accomplished by ejecting an oil/water emulsion into a dispersion fluid under high pressure through an orifice. As a dispersion fluid, 0.2% calcium lactate was appropriate for sodium alginate, while 0.18 sodium hydroxide for chitosan in terms of capsule size and distribution, and emulsion stability. β -cyclodextrin formed inclusion complex with anchovy oil. The microcapsules were stored at 22 and 30℃ for 8 days, and their stability was tested. The acid value remained unchanged and the peroxide value was not detected.