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Cannabidiol-induced apoptosis is mediated by activation of Noxa in human colorectal cancer cells
Jeong, Soyeon,Yun, Hye Kyeong,Jeong, Yoon A,Jo, Min Jee,Kang, Sang Hee,Kim, Jung Lim,Kim, Dae Yeong,Park, Seong Hye,Kim, Bo Ram,Na, Yoo Jin,Lee, Sun Il,Kim, Han Do,Kim, Dae Hyun,Oh, Sang Cheul,Lee, Da Elsevier 2019 Cancer letters Vol.447 No.-
<P><B>Abstract</B></P> <P>Cannabidiol (CBD), one of the compounds present in the marijuana plant, has anti-tumor properties, but its mechanism is not well known. This study aimed to evaluate the apoptotic action of CBD in colorectal cancer (CRC) cells, and focused on its effects on the novel pro-apoptotic Noxa-reactive oxygen species (ROS) signaling pathway. CBD experiments were performed using the CRC cell lines HCT116 and DLD-1. CBD induced apoptosis by regulating many pro- and anti-apoptotic proteins, of which Noxa showed significantly higher expression. To understand the relationship between Noxa and CBD-induced apoptosis, Noxa levels were downregulated using siRNA, and the expression of apoptosis markers decreased. After ROS production was blocked, the level of Noxa also decreased, suggesting that ROS is involved in the regulation of Noxa, which along with ROS is a well-known pro-apoptotic signaling agents. As a result, CBD induced apoptosis in a Noxa-and-ROS-dependent manner. Taken together, the results obtained in this study re-demonstrated the effects of CBD treatment <I>in vivo</I>, thus confirming its role as a novel, reliable anticancer drug.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Our results strongly suggest, for the first time, that CBD can cause Noxa-induced cell death. </LI> <LI> CBD induced apoptotic cell death via ROS/Endoplasmic Reticulum stress-regulated Noxa activation in colorectal cancer cells. </LI> <LI> These results suggest that CBD has important implications for the potential treatment of human CRC. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Da-Hye Shin,Hye-Mi Park,Chan-Seong Jeong,Sang-Hwan Kim 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10
The tyrosinase (TYR) genes have been accepted as major genes involved in the plumage pigmentation of chickens. Tyrosinase (TYR) gene is located on chromosome 1 in chicken and it is composed of five exons and four introns. TYR gene is described as a key enzyme in melanin biosynthesis. Especially, most examples of color patterns in chicken have been due to differential in the tyrosinase gene. This study was conducted to the association of feather color and sequence polymorphism in the Tyrosinase(TYR) gene was investigated using Korean native chickens(red plumage, red-line plumage, Ogol = KNC) and white leghorn(WL). From WL and KNC breed analyses, 232 differential SNPs were detected in 4th exon and 4th intron of TYR gene respectively. The genotype frequencies for 50 SNPs were compared between KCR, KCRD and KCO represented homozygous SNP types in all the analyzed SNP positions while KNC displayed various SNP types. In this study, we conclude that the variation of a wild type sequence in intron 4 of the tyrosinase gene is pigmentation of the original native chickens in korean. This work was supported by a grant from the “Livestock Preservation of Genetic Resources", Rural Development Administration, Republic of Korea.
Involvement of senescence marker protein-30 (SMP30) on the Smad3-dependent hepatic injury
Da-Hee Jeong,Sun-Hee Do,Hai-Jie Yang,Dong-Wei Yuan,Jin-Kyu Park,Il-Hwa Hong,Moon-Jung Goo,Hye-Rim Lee,Mi-Ran Ki,Ho-Yong Park,Kwang-Hee Son,Dong-Ha Sin,Hae-Young Chung,Akihito Ishigami,Kyu-Shik Jeong 한국생명과학회 2006 한국생명과학회 심포지움 Vol.47 No.-
Anticancer activity of extracts of Osmanthus heterophyllus leaves in head and neck cancer cells
Da Hye Jeong,Eugene Cho,Sun Ho Sin,Chan Jin Oh,Deuk Sil Oh,Geon Ung Jo 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
Osmanthus heterophyllus is evergreen shrub belonging to the family of the Oleaceae is known to mainly strengthen the waist and knees. Dried leaves of extracted content (water, 30, 50, 70, 100% alcohol), and then evaporated and lyophilized for use. Total polyphenol and total flavonoid contents of 50% alcohol extract were 45.54, 208.41mg/g, respectively. Verbascoside (29.12, 32.73mg/g, respectively) and rutin (30.59, 36.72 mg/g, respectively) were highly detected in phenolic compound analysis of 50, 70% alcohol extracts using LC-MS/MS. In addition, 50% alcohol extract showed high anticancer activity compared to other alcohol extracts in head and neck cancer cells, but 293T and HaCaT, which are normal cells, did not show cytotoxicity after high concentration treatment. It was confirmed that this cytotoxicity is due to apoptosis through increased annexin V and annexin V/PI stained cells, fragmented PARP, and cleaved caspase-3. Therefore, results suggest that the extract of the potential to be developed as a new drug for anticancer therapy.