複合 韓藥劑 淸籬滋坎湯의 抗癌效果 및 作用機轉 糾明

Cho, Kyung-Sam,Kim, Si-Young,Park, Jai-Kyung,Choi, Seung-Hoon,Chung, Se-Young,Yoon, Hwi-Joong 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Kyung Sam Cho¹Si Young Kim¹, Jai Kyung Park²,Seung Hoon Choi³,Se Young Chung⁴, Hwi Joong Yoon¹¹College of Medicine, ²East-West Medical Research Institute, ³College of Oriental Medicine, ⁴College of Pharmacology, Kyung Hee University,Seoul, Korea. The Anti-cancer Effect of Oriental Medicine Chung-Ri-Ja-Gam-Tang in the Mouse with Metastatic Lung Cancer. Proceedings of International Symposium on East-West Medicine, Seoul. 231-243, 1999.-The oriental medicine Chung-Ri-Ja-Gam-Tang is an herbal medicine which has been used for pulmonary diseases and known as having immune stimulatory effects. It has been known effective in lung cancer. So we studied the effects and the mechanisms of this herbal medicine it the mouse with metastatic lung cancer. The metastatic lung cancer of the mouse was produced with melnoma cell line(B16BL/6).5×10□/mouse tumor cells were injected intravenously to the CDF1 mouse via tail vein. The mice were divided 4 groups. The first group was treated with 50mg/kg extract of Chung-Ri-Ja-Gam-Tang per oral for 10 days after cancer cell injection, second group treated with saline after cancer cell injection, the third group with medicine without cancer cell, and the last group with saline only. After 10 days treatments some of the mice were scarificed and the lung and spleen was removed. The survival duration, weight change, the number of metastatic cancer nodule of the lung, the NK cell activity, the capacity of cytokines(INF-γ,INF-α,IL-2) production and the proliferation activities of mouse lymphocytes were measured. The surival times of the group 1 mice were longer and the weight loss was less than the group 2 significantly. The number of the metastatic nodule of the lung were decresed in the group 1 than the group 2. The production of INF-γ,was increase in group 2 than group 3 and 4, IL-2 production was increased in group 1 than group 2,3,4 significantly. There was no difference in TNF- α production and proliferation activity of lymphocyte in each group. The NK cell activity was significantly increased in group 1 than group 2,3,4. We conclude that the Chung-Ri-Ja-Gam-Tang has the effect of increasing the NK cell activity of the CDF1 mouse with metastatic lung cancer(B16BL/6). And it is suggested that the increased production of the IL-2 is the mechanisms of enhanced NK cell activity.

Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

대장균의 전사조절 유전자 nlp의 분자기구 해석

최용락,정수열,정영기,정정한 東亞大學校附設遺傳工學硏究所 1994 遺傳工學硏究 Vol.- No.1

대장균의 nlp(Ner like protein) 유전자를 클로닝하여 구조해석을 한 결과 전사조절 단백질인 Ner와는 61%의 높은 상동성을 가지고 있음을 이미 보고한 바 있다. 본 연구는 당 대사 관련 유전자의 발현 조절을 보고자 nlp 유전자를 도입시켜 tac promoter에 의해 대량 발현시킴으로서 lactose대사 관련 유전자 lacZ와 maltose 대사 관련 유전자인 malQ, malP의 유전자 발현이 2.5배에서 최고 8.3배 정도까지 증가됨을 확인하였다. 이는 nlp유전자가 cAMP비존재하에 crp*1와 상호작용하여 maltose및 lactose대사를 촉진시킴을 시사한다. nlp-lacZ 융합 유전자 산물을 Immunoblotting하여 분석한 결과 nlp의 promoter가 in vivo 상태에서 발현되어짐을 확인하였다. tac promoter와 IPTG에 의하여 nlp유전자 산물을 대량 발현시킨 결과 약 10,000Da의 산물을 SDS-poly-acrylamide gel 전기영동으로서 확인하였으며, 부분 정제된 Nlp단백질을 조절 영역의 DNA단편에 결합함으로서 전사조절에 관여하는 것으로 사료되어졌다. An nlp (Ner like protein) gene from E. coli was previously cloned and sequenced. Here we show that expression of the sugar metabolism related genes, lacZ, malQ and malP, increased 2.5- to 8.3-fold in the presence of a plasmid containing the nlp gene. This suggested that the nlp gene could induce maltose-and lactose-metabolism coordinately with crp*1 in the absence of CAMP. Using the nlp-lacZ fusion gene, it was possible to show the promoter of nlp was active in vivo. The overexpressed nlp gene product, a polypeptide of 10,000 daltons, was confirmed by SDS-polyacrylamide gel electrophoresis. The band shift assay revealed that the partially purified NIp protein bound a specific DNA of the regulatory region of the nlp gene.

대장균의 전사조절 유전자 nlp의 분자기구 해석

최용락,정수열,정영기,정정한 동의대학교 기초과학연구소 1994 基礎科學硏究論文集 Vol.4 No.1

An nlp (Ner like protein) gene from E. coli was previously cloned and sequenced. Here was show that expression of the sugar metabolism related genes, lacZ, malQ, and malP, increased 2.5- to 8.3-fold in the presence of a plasmid containing the nlp gene. This suggested that the nlp gene could induce maltose- and lactose-metabolism coordinately with crp*1 in the absence of cAMP. Using the nlp-lacZ fusion gene, it was possible to show the promoter of nlp was active in vivo. The overexpressed nlp gene product, a polypeptide of 10,000 daltons, was confirmed by SDS-polyacrylamide gel electrophoresis. The band shift assay revealed that the partially purified Nlp protein bound a specific DNA of the regulatory region of the nlp gene.

Complexity and Algorithms for Optimal Bundle Search Problem with Pairwise Discount

Chung,Jibok,Choi,Byungcheon 한국유통과학회 2019 KODISA ICBE (International Conference on Business Vol.2019 No.-

This paper aims to introduce an optimal bundle search problem (OBSP) which must be solved for online recommendation system to provide an optimized service considering pairwise discount and delivery cost. We propose a mathematical formulation with numerical example for the (OBSP) and analyzed the complexity of the problem.

유효미생물에 의한 하수슬러지의 효율적 처리

최충식,주길재,이동훈,최충렬,이인구,최정 경북대학교 농업과학기술연구소 1999 慶北大農學誌 Vol.17 No.-

This study was conducted to evaluate the effects of microbial inoculation on sewage sludge composting. The number and species of microorganisms in sewage sludge sampled on February were higher than those sampled on August. The composting of sewage sludge is inhibited by the polyacrylamide cation, which was used as a coagulant and known to repress the growth of microorganisms. The growth of all microorganisms was inhibited by the addition of the polyacrylamide cation at a concentration of more than 0.8%. The species and viable counts of microorganisms were observed to increase during composting sewage sludge by inoculation of the effective microorganisms and addition of the pine tree sawdust as a bulking agent, compared with those without inoculation. A variety of organisms in compost(sewage sludge plus sawdust) were observed after composting for 30 days, such as Fragilaria sp., Proales sp., Vorticella sp., Schizothrix sp., Anabaena sp., Zoothaminium sp., Epstylis sp., Arcella sp., Balantidium sp., Actinophrys sp., Synedra sp., Euglypha sp., Ulothrix sp., Anacystis sp., and Clostium sp.

AC 220V 부하전류 2 A가 흐를 때 발생되는 직렬 아크방전의 스펙트라 패턴 해석에 관한 연구

임종민(Jong-Min Lim),최충석(Chung-Seog Choi) 한국화재소방학회 2023 한국화재소방학회 학술대회 논문집 Vol.2023 No.1

단크론항체를 이용한 소 로타바이러스 감염증 치료시험

최해연 ( Hae Yean Choi ),박재명 ( Jae Myong Park ),이은정 ( Un Jeong Lee ),조우영 ( Woo Young Cho ),이종인 ( Jong In Lee ),조부제 ( Bu Jae Cho ),정운선 ( Un Sun Chung ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.21 No.2

To study the efficacy monoclonal antibody(MAb) against bovine rotavirus(BCV) in treatment of calf diarrhea, the MAb was fed to 166 calves with diarrhea from Chung-buk area. The results were summarized as follows. 1. Among the 1,049 calves investigated, 166(16%) calves were infected with BCV. 2. The monthly rate of BCV infection were higher in October to December compared with other months of the year. 3. Among the 166 calves with diarrhea, 137(83%) calves were recovered. 4. Young calves within 7-day-old were more effective in treatment than other ages and the rate of treatment was 86%. 5. Most effective period for treatment of rotavirus was at the first stage of infection.

하악골 골절에 관한 10년간의 추적조사 연구

최현준(Hyun June Choi),정호균(Ho Kyun Chung) 대한구강악안면외과학회 1999 대한구강악안면외과학회지 Vol.25 No.1

This report was a 10year retrospective study on mandible fractures. The study was based on a series of 245 patients who had been treated for mandibular fractures as in-patient at Yongsan Hospital of Medical college of Chung-Ang University during the period of Jan. 1987 to Dec. 1996. The results obtained are as follows; 1. In respect of incidence, the number of fractures was increased to 1990 and then it was decreased, The ratio of Men to Women was 6 : 1. 2. The major etiologic factors were traffic accident(30.2%), fisticuffs(28.6%), falldown(25.3%), accident related to work(7.8%) and sports(6.9%). In recent years, the decreasement of fractures due to traffic accident and the increasement of fractures due to sports was impressive. 3. The age frequency was highst in the third decade(44.9%), and second(26.9%), fourth(13.9%) decade in orders. In the first decade, the cause of fractures was traffic accident and falldown only, and the fractures due to sports were concentrated on second and third decade. 4. The frequent location of mandibular fractures was symphysis(36.1%), left angle(l6.4%), right condyle(10.4%), right angle(8.6%), left condyle(8.6%), left body(5.8%), right body(5.6%), left ramus(4.0%), right ramus(3.8%). The number of average fracture site was 1.62 at each patient and the number of average fracture line was 1.91 at each patient. 5. The simple fracture was 43.7%, and the complex fracture was 56.3%, then the open fracture was 35.5%, and the closed fracture was 64.5%. 6. In respect of treatment, the frequency of open reduction was 74.3% and that of closed reduction was 25.7%, but in the condyle, closed reduction(68.0%) was the more frequently using method of treatment than open reduction(32.0%). 7. The patients were treated an average of 3.27 days after injury and the average hospitalization period was 10.07 days. 8. The mandibutar 3rd molar was existing in 60.6% of angle fractures.

大邱地域 兒童에서 蟯蟲卵 檢出率의 1973年과 1986年과의 比較

徐蓮瓊,金重洛,鄭東一,崔東翊 慶北大學校 醫科大學 1987 慶北醫大誌 Vol.28 No.1

1986年 2月부터 同年 5月까지 大邱近郊의 都市 및 農村國民學校 學童과 孤兒院生의 蟯蟲 感染率을 Graham氏 scotch-tape檢査法으로 1回 檢査한 다음 Choi等(1973)의 調査 成績과 比較하였다. 이번 調査(1986)에서 被檢兒動 936名 中 蟯蟲卵의 檢出率은 27.8%였고 男兒 26.0%, 女兒 29.6%로서 性別間에 有意的 差를 認定할 수 없었으며 年齡이 增加할수록 높았다. 都市兒童에서의 蟯蟲卵의 檢出率은 1973年 43.4%였던 것이 이번(1986)에는 5.4%로서 顯著하게 減少되었다. 그러나 農村學動과 孤兒院生에서는 그 率에 有意的 減少를 나타내었으나 顯著하지 않았다. From February to May, 1986, urban and rural school children and institution children were examined for the presence of Enterobius vermicularis eggs in the Taegu area. All children were examined once by a modification of the Graham's scotch-tape swab technique, and compared with the results reported by Choi et al. (1973). The detection rate in 936 children was found to be 27.8 per cent. The rate in boys was 26.0 per cent, and the rate in girls 29.6 per cent. There was no significant difference in the rate with regard to sex. But the rate among the urban school children in the present study(1986) was only 5.4 per cent, and markedly decreased as compared with the rate, 43.4 per cent reported by Choi et al. (1973). However, difference in the rate of infestation in both rural school children and institution children was not pronounced between 1973 and 1986.