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Oh, Joo Youn,Kim, Mee Kum,Lee, Hyun Ju,Ko, Jung Hwa,Kim, Youngji,Park, Chan-Sik,Kang, Hee Jung,Park, Chung-Gyu,Kim, Sang Joon,Lee, Jin Hak,Wee, Won Ryang Blackwell Publishing Ltd 2010 Xenotransplantation Vol.17 No.2
<P>Oh JY, Kim MK, Lee HJ, Ko JH, Kim Y, Park CS, Kang HJ, Park CG, Kim SJ, Lee JH, Wee WR. Complement depletion with cobra venom factor delays acute cell-mediated rejection in pig-to-mouse corneal xenotransplantation. Xenotransplantation 2010; 17: 140–146. © 2010 John Wiley & Sons A/S.</P><P>Abstract: Background: </P><P>We have demonstrated earlier that porcine corneal xenografts underwent an acute cell-mediated rejection in mice despite the absence of T cells. In the present study, we investigated the effect of complement depletion by cobra venom factor (CVF) on the corneal xenograft rejection in a pig-to-mouse model.</P><P>Methods: </P><P>Porcine corneas were orthotopically transplanted into C57BL/6 (B6) and severe combined immunodeficiency (SCID) mice. For complement depletion, 25 <I>&mgr;</I>g of CVF (1 g/kg bodyweight) was injected intraperitoneally on the day before and 1, 3, 5, and 7 days after transplantation. Graft survival was clinically assessed by slit lamp biomicroscopy and the median survival time (MST) was calculated. The grafts were histologically evaluated serially after transplantation using antibodies against CD4, CD8, NK1.1, and F4/80.</P><P>Results: </P><P>The CVF treatment significantly prolonged the porcine corneal xenograft survival in both B6 (MST 9.4 vs. 15.5 days; P = 0.0011) and SCID mice (MST 16.4 vs. 20.5 days; P = 0.0474). Histologically, whereas macrophages and CD4<SUP>+</SUP> T cells were progressively infiltrated into porcine corneal grafts in CVF-untreated B6 mice, the infiltration by both cells was markedly delayed and decreased in the xenografts in CVF-treated B6 mice. Likewise, macrophage infiltration, which was prominent in rejected porcine xenografts in SCID mice, was also reduced in CVF-treated SCID mice.</P><P>Conclusions: </P><P>Our results suggest that complement depletion by CVF delayed, although did not prevent, an acute cell-mediated rejection in a pig-to-mouse corneal xenotransplantation.</P>
Effect of αGal on corneal xenotransplantation in a mouse model
Choi, Hyuk Jin,Kim, Mee Kum,Lee, Hyun Ju,Jeong, So Hee,Kang, Hee Jung,Park, Chan‐,Sik,Park, Chung‐,Gyu,Joon Kim, Sang,Wee, Won Ryang Blackwell Publishing Ltd 2011 Xenotransplantation Vol.18 No.3
<P>Choi HJ, Kim MK, Lee HJ, Jeong SH, Kang HJ, Park C‐S, Park C‐G, Kim SJ, Wee WR. Effect of αGal on corneal xenotransplantation in a mouse model. Xenotransplantation 2011; 18: 176–182. © 2011 John Wiley & Sons A/S.</P><P><B>Abstract: </B><B> Background: </B> It has been reported that hyperacute rejection (HAR) does not occur after pig‐to‐nonhuman corneal xenotransplantation. However, considering that immune privilege is already disrupted in most human corneal recipients, and the expression of αGal can be gradually reduced after pig‐to‐rat corneal transplantation, the long‐term survival of corneal grafts from wild‐type pigs cannot be guaranteed. Accordingly, we aimed to investigate the effect of αGal on the change in anti‐Gal antibodies, using sensitized α1,3‐galactosyltransferase gene‐knockout (GTKO) mice recipients.</P><P><B>Methods: </B> C57BL/6 (B6) and GTKO mice were divided into 5 groups and underwent orthotopically full thickness cormeal transplantation as follows (n=4 for each group): (1) group 1: B6 to B6; (2) group 2: fresh porcine posterior corneal lamella to B6; (3) fresh porcine posterior corneal lamella to GTKO; (4) group 4: decellularized porcine posterior corneal lamella to GTKO, and (5) group 5: B6 to GTKO. Before transplantation, all GTKO recipients were sensitized using intraperitoneal injections of rabbit blood cells. Median survival times (MST) for the corneal grafts of the different groups were compared and plasma concentrations of IgG/IgM anti‐Gal antibodies were evaluated at 1 week, 2 weeks and 3 weeks post‐transplantation.</P><P><B>Results: </B> There were no differences in MSTs between groups. Although there was no HAR of fresh porcine posterior corneal grafts even in sensitized GTKO recipients, αGal expression was induced in the transplanted fresh porcine corneal grafts and plasma concentration of IgG anti‐Gal antibody was gradually increased in fresh porcine cornea‐grafted GTKO recipients. On the contrary, αGal expression did not increase in the grafts and plasma concentration of anti‐Gal antibodies did not change after transplantation using decellularized porcine corneas.</P><P><B>Conclusions: </B> Our findings suggest that αGal may affect the long‐term survival of porcine corneal xenografts via antibody‐mediated rejection, although αGal does not have an effect on acute rejection and decellularized porcine corneas may enable the long‐term survival of porcine corneal xenografts.</P>
박찬량 국민대학교 2003 기초과학연구소 논문집 Vol.22 No.-
266㎚ 광분해로부터 생성된 들뜬 전자생태의 산소원자(O(¹D₂))와 H₂로부터 생성된 OH의 내부에너지 분포를 레이저유발 형광법을 이용하여 연구하였다. O(¹D₂)와 H₂의 반응은 매우 들뜬 회전 및 진동상태의 OH를 생성하였다. 또한 생성된 OH는 ∏(A′)Δ 레벨에 대한 선호도를 나타내었으며 이는 OH 생성물의 회전평면이 반응 중간체인 H₂O의 분자평면과 일치함을 의미한다. The distributions of the internal energies of nascent OH produced from the reaction of O(¹D₂) with H₂ was investigated using laser induced fluorescence technique. O(¹D₂) was produced from the photolysis of O₃ at 266nm. Reaction of O(¹D₂) with H₂ yields highly vibrationally and rotationally excited OH displaying the propencity for ∏(A’) A sublevel production characteristic of reactions in which the rotational plane of the OH product coincides with the plane of a triatomic intermediate complex.
Tubulin Polymerization에 대한 Microtubule Associated DNA Binding Protein 의 영향
박희찬,권기량,황병두 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.2
Although the involvement of tubulin in the segregation of chromosome during mitosis is well established, the role of DNA binding protein(DBP) in tubulin polymerization is unclear. When the microtubule was prepared from rabbit brain through 2 cycle of glycerol-and GTP-mediated polymerization, the preparation(MADBP^+) was found to have serveral DBP. These DBP were separated from tubulin using double strand DNA cellulose column chromatography and the proper-ties of this tubulin polymerizatin could be inhibited by DNA whereas MADBP^- polymerization could not. Addition of microtubule associated DNA binding protein(MADBP) to MADBP^- could change MA-DBP^- polymerization kinetics; some MADBP fractions decreased the initiation and increased the elongation, some fractions simply increased the polymerization. From the above results, the possible roles of MADBP and possible binding site of taxol were discussed.
Park, Hea Jung,Kim, Ji Na,Yoo, Hyun-Ji,Wee, Kyung-Ryang,Kang, Sang Ook,Cho, Dae Won,Yoon, Ung Chan American Chemical Society 2013 Journal of organic chemistry Vol.78 No.16
<P>On the basis of the results of frontier orbital considerations, 4-substituted-2′-pyridyltriazoles were designed to serve as ancillary ligands in 2-phenylpyridine main ligand containing heteroleptic iridium(III) complexes that display deep blue phosphorescence emission. The iridium(III) complexes, <B>Ir1</B>–<B>Ir7</B>, prepared using the new ancillary ligands, were found to display structured, highly quantum efficient (Φ<SUB>p</SUB> = 0.20–0.42) phosphorescence with emission maxima in the blue to deep blue 448–456 nm at room temperature. In accord with predictions based on frontier orbital considerations, the complexes were observed to have emission properties that are dependent on the electronic nature of substituents at the C-4 position of the pyridine moiety of the ancillary ligand. Importantly, placement of an electron-donating methyl group at C-4′ of the pyridine ring of the 5-(pyridine-2′-yl)-3-trifluoromethyl-1,2,4-triazole ancillary ligand leads to an iridium(III) complex that displays a deep blue phosphorescence emission maximum at 448 nm in both the liquid and film states at room temperature. Finally, an OLED device, constructed using an Ir-complex containing the optimized ancillary ligand as the dopant, was found to emit deep blue color with a CIE of 0.15, 0.18, which is close to the perfect goal of 0.15, 0.15.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/joceah/2013/joceah.2013.78.issue-16/jo4012514/production/images/medium/jo-2013-012514_0013.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jo4012514'>ACS Electronic Supporting Info</A></P>
Park, Ji-Won,Lee, In-Chul,Shin, Na-Rae,Jeon, Chan-Mi,Kwon, Ok-Kyoung,Ko, Je-Won,Kim, Jong-Choon,Oh, Sei-Ryang,Shin, In-Sik,Ahn, Kyung-Seop Informa UK (Informa Healthcare) 2016 Nanotoxicology Vol.10 No.4
<P>Copper oxide nanoparticles (CuONPs), metal oxide nanoparticles were used in multiple applications including wood preservation, antimicrobial textiles, catalysts for carbon monoxide oxidation and heat transfer fluid in machines. We investigated the effects of CuONPs on the respiratory system in Balb/c mice. In addition, to investigate the effects of CuONPs on asthma development, we used a murine model of ovalbumin (OVA)-induced asthma. CuONPs markedly increased airway hyper-responsiveness (AHR), inflammatory cell counts, proinflammatory cytokines and reactive oxygen species (ROS). CuONPs induced airway inflammation and mucus secretion with increases in phosphorylation of the MAPKs (Erk, JNK and p38). In the OVA-induced asthma model, CuONPs aggravated the increased AHR, inflammatory cell count, proinflammatory cytokines, ROS and immunoglobulin E induced by OVA exposure. In addition, CuONPs markedly increased inflammatory cell infiltration into the lung and mucus secretions, and MAPK phosphorylation was elevated compared to OVA-induced asthmatic mice. Taken together, CuONPs exhibited toxicity on the respiratory system, which was associated with the MAPK phosphorylation. In addition, CuONPs exposure aggravated the development of asthma. We conclude that CuONPs exposure has a potential toxicity in humans with respiratory disease.</P>
Kinetics of the Photochemically Generated t-Butoxy Radical Reactions with Phosphine(PH$_3)^*$
박찬량,추광율,Park Chan Ryang,Choo Kwang Yul Korean Chemical Society 1985 Bulletin of the Korean Chemical Society Vol.6 No.4
The gas phase reactions of the photochemically generated t-butoxy radicals with phosphine ($PH_3$) were studied in the temperature range of $35-80^{\circ}C.$ We found the significant differences between high temperature thermal reactions and low temperature photo reactions. In comparison with the reactions of t-butoxy radicals with other hydrogen donors, some possible mechanistic suggestions were made for the explanation of the results.
Lim, Kyu,Park, Hee-Chan,Kim, Kye-Young,Lee, Myung-Sun,Kweon, Gi-Ryang,Kwak, Sang-Tae,Hwang, Byung-Doo 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-
We have investigated DNA synthesis and levels of H2B histone mRNA, and the binding pattern of nuclear proteins to various elements in the rat H2B histone gene upstream region with DNase I footprinting assay. Both DNA synthesis and H2B histone mRNA level were increased with maximal stimulation reached at 24 hrs and 36 hrs after partial hepatectomy, respectively. In DNase I footprinting analysis, the nuclear factors interacting with the three elements, TATA at - 19 bp (site A^R), site B at-29 bp, and CAAT at-69 bp (site C) were required during maximal increase of H2B histone mRNA level after partial hepatectomy. The DNase I protection pattern by nuclear extract of the cycloheximide-treated regenerating liver showed the same result with normal liver. These results suggest that transcriptional regulation of H2B histone gene during liver regeneration may be mediated by nuclear factors that are newly induced by paritial hepatectomy.