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Won Sung Min,Lee Na Young,Oh Ki-Kwang,Gupta Haripriya,Sharma Satya Priya,Kim Kyung Hwan,Kim Byoung Kook,Joung Hyun Chae,Jeong Jin Ju,Ganesan Raja,Han Sang Hak,Yoon Sang Jun,Kim Dong Joon,Suk Ki Tae 한국미생물학회 2023 The journal of microbiology Vol.61 No.2
The progression and exacerbation of liver fibrosis are closely related to the gut microbiome. It is hypothesized that some probiotics may slow the progression of liver fibrosis. In human stool analysis [healthy group (n = 44) and cirrhosis group (n = 18)], difference in Lactobacillus genus between healthy group and cirrhosis group was observed. Based on human data, preventive and therapeutic effect of probiotics Lactobacillus lactis and L. rhamnosus was evaluated by using four mice fibrosis models. L. lactis and L. rhamnosus were supplied to 3,5-diethoxycarbonyl-1,4-dihydrocollidine or carbon tetrachloride-induced liver fibrosis C57BL/6 mouse model. Serum biochemical measurements, tissue staining, and mRNA expression in the liver were evaluated. The microbiome was analyzed in mouse cecal contents. In the mouse model, the effects of Lactobacillus in preventing and treating liver fibrosis were different for each microbe species. In case of L. lactis, all models showed preventive and therapeutic effects against liver fibrosis. In microbiome analysis in mouse models administered Lactobacillus, migration and changes in the ratio and composition of the gut microbial community were confirmed. L. lactis and L. rhamnosus showed preventive and therapeutic effects on the progression of liver fibrosis, suggesting that Lactobacillus intake may be a useful strategy for prevention and treatment.
Plant Regeneration Depending on Explant Type in Chrysanthemum coronarium L.
Chae Won-Byoung,Choi Geun-Won,Chung In-Sik The Korean Society of Plant Biotechnology 2004 Plant molecular biology and biotechnology research Vol.6 No.4
Plant regeneration depending on explant type was inves-tigated with cotyledon, hypocotyl, and leaf explants of garland chrysanthemum (Chrysanthemum coronarium L.) cultured on MS basal medium supplemented with various concentrations of SAP and NAA combination. Among the three different types of explants, hypocotyl explants grown on MS medium containing $1.0{\mu}M\;NAA,\;1.0{\mu}M\;BA\;and\;6{\mu}M\;AgNO_3$ produced the highest adventitious shoots (4.67 per explant). Hypocotyl explants not only produced more vigorous shoots, which regenerated aster than the cotyledon and leaf explants. An efficient root formation was observed in MS medium containing $3\%$sucrose. The concentration of NAA did not show significant effects on root formation. Results from this experiment suggested that hypocotyl explants were efficient for the regeneration of garland chrysanthemum.
Won Byoung Chae,Hong Lim Kim,Haksoon Choi,Yong Bum Kwack,Dong Woo Lee 한국원예학회 2008 Horticulture, Environment, and Biotechnology Vol.49 No.6
This experiment was performed to investigate the effects of seed tuber sources and sizes on the growth, yield and tuber size distribution of potato ‘Dejima’. Mini-tubers from aeroponics (MT) were classified according to their weight (5±0.5, 10±1.0, 20±2.0, 30±3.0, and 40±4.0 g) and compared with conventional tubers from field condition (CT) of the same weights. About two-fold increase in the number of eyes per seed tuber was observed in MT when compared to CT and the number of eyes increased with increasing seed tuber size in both seed tuber sources. The plant height and tuber fresh weight of CT tended to be higher than those of MT but the numbers of main stems and tubers were higher in MT than in CT. Tuber fresh weight and main stem number tended to increase as seed tuber size increased in both seed sources. The numbers of main stems and tubers highly correlated with the number of eyes. Tuber size distribution was affected by seed sources and MT generally produced smaller tubers than CT.
Agrobacterium-mediated Seed Transformation System in Garland Chrysanthemum
Chae, Won-Byoung,Choi, Geun-Won,Chung, In-Sik 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-
Agrobacterium을 이용한 쑥갓 종자 형질전환이 수행되었다. 상처 처리한 종자와 무처리 종자를 terpene cyclase 유전자가 재조합된 pILTAB vector를 갖는 Agrobacterium tumefaciens LBA4404와 5일 동안 공동배양하였고 공동배양한 종자들은 kanamycin 172 μM과 cefotaxime 420 μM이 첨가된 선발배지에서 배양되었다. 상처 처리한 종자는 무처리 종자에 비하여 높은 발아율을 보였고 상처 처리한 종자의 유묘가 형질전환 된 것으로 추정되는 식물체를 생산한 반면에 무처리한 종자의 유묘는 선발배지에서 고사하였다. Terpene cyclase 유전자 특이 primer를 이용한 PCR 분석을 실시한 결과, 선발배지에서 생장한 7개 중 1개의 쑥갓에서 1.64 kb 크기의 terpene cyclase 유전자의 성공적인 도입을 확인하였다. A transgenic garland chrysanthemum plant was successfully produced through Agrobacterium-mediated seed transformation system. Both intact and cracked seeds were co-cultivated for 5 days with A. tumeficiens strain LBA4404 carrying binary vector pILTAB 357 including 1.64 kb terpene cyclase gene isolated from sweet annie (Artemisia annua L.) and cultured on selection medium supplemented with 172 μM of kanamycin and 420 μM of cefotaxime to select putative transgenic seedlings. Germination rate of cracked seeds was higher than that of intact seeds and seedlings from cracked seeds developed into putative transgenic plants whereas seedlings from intact seeds were blighted on the selection medium. Results from PCR analysis with terpene cyclase gene-specific primers confirmed a successful insertion of 1.64 kb terpene cyclase gene into 1 seedling among 7 seedlings grown on the selection medium.