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Cho, Byoung Ok,Che, Denis Nchang,Yin, Hong Hua,Shin, Jae Young,Jang, Seon Il Elsevier 2017 BIOMEDICINE AND PHARMACOTHERAPY Vol.89 No.-
<P><B>Abstract</B></P> <P>Atopic dermatitis, a chronic relapsing and pruritic inflammation of the skin also thought to be involved in, or caused by immune system destruction is an upsetting health problem due to its continuously increasing incidence especially in developed countries. Mast cell infiltration in atopic dermatitis skin lesions and its IgE-mediated activation releases various cytokines and chemokines that have been implicated in the pathogenesis of atopic dermatitis. This study was aimed at investigating synergistic anti-inflammatory, anti-pruritic and anti-atopic dermatitis effects of <I>Diospyros lotus</I> leaf extract (DLE) and Muscat bailey A grapefruit stem extract (GFSE) in atopic dermatitis-like induced skin lesions in mice. Combinations of DLE and GFSE inhibited TNF-α and IL-6 production more than DLE or GFSE in PMA plus calcium ionophore A23187-activated HMC-1 cells. DLE and GFSE synergistically inhibited compound 48/80-induced dermal infiltration of mast cells and reduced scratching behavior than DLE or GFSE. Furthermore, DLE and GFSE synergistically showed a stronger ameliorative effect in skin lesions by reducing clinical scores; dermal infiltration of mast cells; ear and dorsal skin thickness; serum IgE and IL-4 production in atopic dermatitis-like mice. Collectively, these results suggest that DLE and GFSE synergistically exhibit anti-atopic dermatitis effects in atopic dermatitis-like skin lesions in mice.</P>
CHO, Byoung Ok,JIN, Chang Hyun,PARK, Yong Dae,RYU, Hyung Won,BYUN, Myung Woo,SEO, Kwon Il,JEONG, Il Yun Japan Society for Bioscience, Biotechnology, and A 2011 Bioscience, biotechnology, and biochemistry Vol.75 No.7
<P>Isoegomaketone (IK) is an essential oil component of <I>Perilla frutescens</I> (L.), but the mechanism by which IK induces apoptosis has never been studied. The purpose of this study was to elucidate the IK-induced apoptotic pathway in DLD1 human colon cancer cells. We observed that IK treatment over 24 h significantly inhibited cell viability in a dose-dependent manner. We also found that IK triggered cleavage of PARP. Moreover, IK treatment resulted in cleavage of caspase-8, -9, and -3 in a dose- and time-dependent manner. IK treatment also resulted in cleavage of Bid and translocation of Bax, and triggered the release of cytochrome <I>c</I> from the mitochondria to the cytoplasm. Furthermore, it resulted in the translocation of apoptosis inducing factor (AIF), a caspase-independent mitochondrial apoptosis factor, from the mitochondria into the nucleus. Overall, these results suggest that IK induces apoptosis through caspase-dependent and capase-independent pathways in DLD1 cells.</P>
Induction of apoptosis by 2,3-dehydrosilybin via a caspase-dependent pathway in human HeLa cells.
Cho, Byoung Ok,So, Yangkang,Jin, Chang Hyun,Byun, Myung Woo,Seo, Kwon Il,Ko, Kisung,Chun, Myoung Sook,Jeong, Il Yun Japan Society for Bioscience, Biotechnology, and A 2014 Bioscience, Biotechnology, and Biochemistry Vol.78 No.2
<P>The aim of this study was to investigate the mechanisms involved in the apoptosis of HeLa cells due to 2,3-dehydrosilybin (DHS) treatment. DHS treatment over 24 h significantly inhibited cell viability and induced apoptosis in a dose-dependent manner. It also triggered the cleavage of caspase-8, caspase-9, caspase-3, and PARP, and significantly increased caspase-3 activity in a dose-dependent manner. Moreover, it triggered the depolarization of the mitochondrial membrane potential (δψm), the release of cytochrome c into the cytosol, the cleavage of Bid, and the downregulation of Bcl-2 in a dose-dependent manner. Furthermore, z-VAD-fmk (a pan-caspase inhibitor) and z-IETD-fmk (a specific caspase-8 inhibitor) abolished the DHS-induced activation of the caspase-8, -9, and -3, cleavage of PARP, the depolarization of δψm, the release of cytochrome c, the cleavage of Bid, and the downregulation of Bcl-2. Taken together, these results suggest that DHS-induced apoptosis is mediated by a caspase-dependent pathway in human HeLa cells.</P>
Byoung Ok Cho,Jae Young Shin,Ji Hyeon Park,Feng Wang,Suping Hao,Da Jeong Shin,Seon Il Jang 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7
Chronic pruritus is a symptom that reduces the quality of life of patients with inflammatory skin disease. Persistent activation of astrocytic signal transducer and activator of transcription 3 (STAT3) contributes to the elevation of chronic pruritus. STAT3 activation increases lipocalin-2 (LCN2) expression and enhances pruritus. A 2,3-dehydrosilybin (DHS) is a type of flavonoid extracted from the seeds of milk thistle. DHS has been reported to have hepatoprotective, angiogenic, and antioxidant effects. In this study, the inhibitory effect of DHS on chronic pruritus was investigated in IL-6-treated astrocytes and chloroquine-injected mice. As a result, DHS prevented STAT3 activation and LCN2 production in IL-6-treated astrocytes. Moreover, DHS inhibited scratching and inhibited the expression of glial fibrillary acidic protein (GFAP) in chloroquine-injected mice. It also reduced the level of inflammatory cytokines in the mice serum. In conclusion, it was demonstrated that DHS suppressed itch through the STAT3 signaling pathway. Thus our results suggest that DHS can prevent and/or treat chronic itch.
Cho, Byoung Ok,Che, Denis Nchang,Shin, Jae Young,Kang, Hyun Ju,Jang, Seon Il Elsevier 2018 Biomedicine & pharmacotherapy Vol.97 No.-
<P><B>Abstract</B></P> <P>This study analyzed fruit stem extract (MGFE) from Muscat Bailey A grape (<I>Vitis labrusca </I>× <I>Vitis vinifera</I>) for their ameliorative effects on Ultraviolet B (UVB)-induced skin damage in Balb/c mice. Well established <I>in vivo</I> assays were used to determine the biological effects of MGFE upon UVB irradiation of BALB/c mice. The results showed that treatment with MGFE recovered glutathione depletion, prevented lipid peroxidation of tissues and decreased the expression of DNA repair enzyme oxo guanine glycosylase-1. MGFE recovered the skin conditions in UVB-irradiated Balb/c mice. Moreover, MGFE inhibited dermal infiltration of inflammatory cells and reduced serum tumor necrosis factor alpha and interleukin-6 levels. Finally, MGFE treatment inhibited UV<B>B</B>-induced melanin formation and collagen fiber destruction through the inhibition of matrix metalloproteinase-1 expression. Through high-performance liquid chromatography analysis, catechin, epicatechin, and <I>trans</I>-resveratrol were found to be among the main active compounds present in MGFE. Taken together, these results indicated that MGFE has potentials as topical therapeutic materials against skin damage by inhibiting oxidative stress and inflammatory.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Using UVB irradiation, we induced oxidative stress in Balb/c mice skin. </LI> <LI> MGFE recovered skin damages induced by UVB irradiation. </LI> <LI> MGFE has the potential to be a natural candidate agent for the treatment of skin photo damage. </LI> </UL> </P>