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조문경(Moon-Kyoung Cho),구본비(Bon-Bi Koo),김소희(So-Hee Kim) 한국색채학회 2015 한국색채학회 학술대회 Vol.2015 No.11
인간과 환경과의 관계를 새롭게 인식하면서 나타난 공간 환경 개선을 위한 디자인은 지역 주민의 삶을 향상시키고 지역의 정체성을 되찾아 활력을 줄 수 있는 가능성을 가진다. 본 연구는 최근 변화하는 농어촌의 다양한 사회적 요구에 대응하여 지역의 특색을 담은 공간 환경개선에 관한 방안을 찾아가는 기초적 작업이다. 농어촌 환경 개선을 위한 정책과 방향을 통해 우선적으로 환경개선을 위한 공간 대상을 선정 분류하고 이러한 대상에 대한 환경개선 방법의 범주를 제시한다. 이것은 농어촌의 구조개선과 지역 주민의 삶의 질을 개선시키는 방향으로 농어촌 경관의 획일화와 기능 저하를 극복할 수 있다. 본 연구에서는 농어촌 환경개선의 대상과 방법을 제시하여 보다 쾌적한 농어촌 환경을 조성하고자 한다.
Notch1 counteracts WNT/β-catenin signaling through chromatin modification in colorectal cancer.
Kim, Hyun-A,Koo, Bon-Kyoung,Cho, Ji-Hoon,Kim, Yoon-Young,Seong, Jinwoo,Chang, Hee Jin,Oh, Young Min,Stange, Daniel E,Park, Jae-Gahb,Hwang, Daehee,Kong, Young-Yun American Society for Clinical Investigation 2012 The Journal of clinical investigation Vol.122 No.9
<P>Crosstalk between the Notch and wingless-type MMTV integration site (WNT) signaling pathways has been investigated for many developmental processes. However, this negative correlation between Notch and WNT/β-catenin signaling activity has been studied primarily in normal developmental and physiological processes in which negative feedback loops for both signaling pathways are intact. We found that Notch1 signaling retained the capability of suppressing the expression of WNT target genes in colorectal cancers even when β-catenin destruction by the adenomatous polyposis coli (APC) complex was disabled. Activation of Notch1 converted high-grade adenoma into low-grade adenoma in an Apcmin mouse colon cancer model and suppressed the expression of WNT target genes in human colorectal cancer cells through epigenetic modification recruiting histone methyltransferase SET domain bifurcated 1 (SETDB1). Extensive microarray analysis of human colorectal cancers also showed a negative correlation between the Notch1 target gene, Notch-regulated ankyrin repeat protein 1 (NRARP), and WNT target genes. Notch is known to be a strong promoter of tumor initiation, but here we uncovered an unexpected suppressive role of Notch1 on WNT/β-catenin target genes involved in colorectal cancer.</P>
Choi, Bok Ryul,Koo, Bon Chul,Ahn, Kwang Sung,Kwon, Mo Sun,Kim, Jin-Hoi,Cho, Seong-Keun,Kim, Kyoung Mi,Kang, Jee Hyun,Shim, Hosup,Lee, Hyuna,Uhm, Sang Jun,Lee, Hoon Taek,Kim, Teoan JOHN WILEY & SONS LTD 2006 MOLECULAR REPRODUCTION AND DEVELOPMENT Vol.73 No.10
<P>A critical problem of transgenic livestock production is uncontrollable constitutive expression of the foreign gene, which usually results in serious physiological disturbances in transgenic animals. One of the best solutions for this problem may be use of controllable gene expression system. In this study, using retrovirus vectors designed to express the enhanced green fluorescent protein (EGFP) gene under the control of the tetracycline-inducible promoter, we examined whether the expression of the transgene could be controllable in fibroblast cells and nuclear transfer (NT) embryos of porcine. Transformed fibroblast cells were cultured in medium supplemented with or without doxycycline (a tetracycline analog) for 48 hr, and the induction efficiency was measured by comparing EGFP gene expression using epifluorescence microscopy and Western and Northern blot analyses. After the addition of doxycycline, EGFP expression increased up to 17-fold. The nuclei of transformed fibroblast cells were transferred into enucleated oocytes. Fluorescence emission data revealed strong EGFP gene expression in embryos cultured with doxycycline, but little or no expression in the absence of the antibiotic. Our results demonstrate the successful regulation of transgene expression in porcine nuclear transfer embryos, and support the application of an inducible expression system in transgenic pig production to solve the inherent problems of side-effects due to constitutive expression of the transgene. Mol. Reprod. Dev. © 2006 Wiley-Liss, Inc.</P>
Cheon, So Y.,Cho, Kyoung J.,Kim, So Y.,Kam, Eun H.,Lee, Jong E.,Koo, Bon-Nyeo Frontiers Media S.A. 2016 Frontiers in cellular neuroscience Vol.10 No.-
<P>Conditions of increased oxidative stress including cerebral ischemia can lead to blood–brain barrier dysfunction via matrix metalloproteinase (MMP). It is known that MMP-9 in particular is released from brain endothelial cells is involved in the neuronal cell death that occurs after cerebral ischemia. In the intracellular signaling network, apoptosis signal-regulating kinase 1 (ASK1) is the main activator of the oxidative stress that is part of the pathogenesis of cerebral ischemia. ASK1 also promotes apoptotic cell death and brain infarction after ischemia and is associated with vascular permeability and the formation of brain edema. However, the relationship between ASK1 and MMP-9 after cerebral ischemia remains unknown. Therefore, the aim of the present study was to determine whether blocking ASK1 would affect MMP-9 activity in the ischemic brain and cultured brain endothelial cells. Our results showed that ASK1 inhibition efficiently reduced MMP-9 activity <I>in vivo</I> and <I>in vitro</I>. In endothelial cell cultures, ASK1 inhibition upregulated phosphatidylinositol 3-kinase/Akt/nuclear factor erythroid 2 [NF-E2]-related factor 2/heme oxygenase-1 signals and downregulated cyclooxygenase-2 signals after hypoxia/reperfusion. Additionally, in neuronal cell cultures, cell death occurred when neurons were incubated with endothelial cell-conditioned medium (EC-CM) obtained from the hypoxia/reperfusion group. However, after incubation with EC-CM and following treatment with the ASK1 inhibitor NQDI-1, neuronal cell death was efficiently decreased. We conclude that suppressing ASK1 decreases MMP-9 activity in brain endothelial cells, and leads to decreased neuronal cell death after ischemic injury.</P>
Jeong, Hyun-Woo,Jeon, Un Sil,Koo, Bon-Kyoung,Kim, Wan-Young,Im, Sun-Kyoung,Shin, Juhee,Cho, Yunje,Kim, Jin,Kong, Young-Yun American Society for Clinical Investigation 2009 The Journal of clinical investigation Vol.119 No.11
<P>The heterogeneous cellular composition of the mammalian renal collecting duct enables regulation of fluid, electrolytes, and acid-base homeostasis, but the molecular mechanism of its development has yet to be elucidated. The Notch signaling pathway is involved in cell fate determination and has been implicated in proximal-distal patterning in the mammalian kidney. To investigate the role of Notch signaling in renal collecting duct development, we generated mice in which Mind bomb-1 (Mib1), an E3 ubiquitin ligase required for the initiation of Notch signaling, was specifically inactivated in the ureteric bud of the developing kidney. Mice lacking Mib1 in the renal collecting duct displayed increased urinary production, decreased urinary osmolality, progressive hydronephrosis, sodium wasting, and a severe urinary concentrating defect manifested as nephrogenic diabetes insipidus. Histological analysis revealed a diminished number of principal cells and corresponding increase in the number of intercalated cells. Transgenic overexpression of Notch intracellular domain reversed the altered cellular composition of mutant renal collecting duct, with principal cells occupying the entire region. Our data demonstrate that Notch signaling is required for the development of the mammalian renal collecting duct and principal cell differentiation and indicate that pathway dysregulation may contribute to distal renal tubular disorders.</P>
카바프로스타싸이클린 중간체의 합성: 1-Alkoxy-benzenesulfonyl-cyano-4-ethenyl cyclopeptane의 입체선택적 합성
서영거(Young Ger Suh),정재경(Jae Kyoung Jung),구본암(Bon Am Koo),최영기(Young Gi Choi),조윤상(Youn Sang Cho) 대한약학회 1995 약학회지 Vol.39 No.1
An efficient and stereoselective synthetic route to the trisubstituted hydroxy cyclopentane as an useful synthetic intermediate for carbaprostacyclin is described. Pd(O)-mediated intramolecular alkylation of allylic carbonate has been employed as a key reaction.
Receptor Activator of NF-κB Ligand Regulates the Proliferation of Mammary Epithelial Cells via Id2
Kim, Nam-Shik,Kim, Hyun-Ju,Koo, Bon-Kyoung,Kwon, Min-Chul,Kim, Young-Woong,Cho, Yunje,Yokota, Yoshifumi,Penninger, Josef M.,Kong, Young-Yun American Society for Microbiology 2006 Molecular and cellular biology Vol.26 No.3
<B>ABSTRACT</B><P>Receptor activator of NF-κB ligand (RANKL) is a key regulator for mammary gland development during pregnancy. RANKL-deficient mice display impaired development of lobulo-alveolar mammary structures. Similar mammary gland defects have been reported in mice lacking Id2. Here we report that RANKL induces the proliferation of mammary epithelial cells via Id2. RANKL triggers marked nuclear translocation of Id2 in mammary epithelial cells. In vivo studies further demonstrated the defective nuclear translocation of Id2, but the normal expression of cyclin D1, in the mammary epithelial cells of <I>rankl</I><SUP><I>−/−</I></SUP> mice. In vitro studies with nuclear localization sequence-tagged Id2 revealed that the nuclear localization of Id2 itself is critical for the downregulation of p21 promoter activity. Moreover, RANKL stimulation failed to induce cell growth and to downregulate p21 expression in <I>Id2</I><SUP><I>−/−</I></SUP> mammary epithelial cells. Our results indicate that the inhibitor of helix-loop-helix protein, Id2, is critical to control the proliferation of mammary epithelial cells in response to RANKL stimulation.</P>
차준민(Jun-Min Cha),구본희(Bon-Hui Ku),윤경하(Kyoung-Ha Yoon),조범섭(Burm-Sup Cho),변성현(Sung-Hyun Byun) 대한전기학회 2010 대한전기학회 학술대회 논문집 Vol.2010 No.7
발전기를 통한 무효전력의 공급은 공급가능한 무효전력의 능력의 사전예측이 가능하며 중앙의 지시를 받아 운영가능한 무효전력의 공급원이다. 무효전력의 공급은 계통에서 송전손실을 감소하고 모선전압을 기준 범위 내에서 유지하여 소비자들의 기기 사용을 보호하는 역할을 한다. 본 연구에서는 발전기 출력 특성자료의 최대 유효전력(Pmax)값에 해당하는 발전기출력가능곡선의 P-Q 곡선에서 한계값을 찾고, 이를 통하여 발전원 별 공급가능한 무효전력 용량과 발전기의 무효전력 공급능력에 대하여 알아보았다.
과천시 초등학교 학생들의 간식섭취 양상에 관한 조사연구
이영희,김권수,조본경,권호근,김영옥,김영남 대한구강보건학회 1999 大韓口腔保健學會誌 Vol.23 No.2
Dental caries has not been controlled appropriately in Korea. Changes of diet habit, especially increase of between-meal intake in children has been considered as one reason of this prevalent caries. The purpose of this study was to investigate the pattern of between-meal intake in elementary schoolchildren in order to provide the basic data for future study on the relationship of dental caries and between-meal intake and to use this results for dental health education. We examined the pattern of between-meal intake for 4 days including weekend by food frequency questionnaire for Kwachun elementary schoolchildren in 1995, 1996. The results were as follows; 1. Most popular snack to elementary schoolchildren was milk. Frequency of children's between-meal intake was more than 8 per day and that of eating cariogenic snack more than 3 per day in both 1995 and 1996 survey. 2. Frequency of between-meal intake of boys was higher than that of giris(p<0.05) in 1996 survey, and the frequency of between-meal intake in higher grade schoolchlldren was lower than that in lower grade schoolchildren(p<0.05) in both 1995 and 1996 survey. 3. Frequency of between-meal intake in children whose mother had a job was lower than that in chffdren whose mother had not a job(p<0.05) in 1996 survey. 4. Frequency of between-meal intake in a weekend was higher than that in a weekday, and proportion of cariogenic snack among between-meal intake in a weekend was also higher than that in the weekday(p<0.05) in both 1995 and 1996 survey.