용담의 기내 개화 및 증식에 관한 연구

손병구,최영환,안종길,조동,권오창,박정기,Son, Beung-Gu,Choi, Young-Whan,Ahn, Chong-Kil,Cho, Dong,Kwon, Oh-Chang,Park, Jung-Ki Korean Society of Life Science 1996 생명과학회지 Vol.6 No.1

Experiments were conducted to determine the effects of plant growth regulators on in vitro flowering and micropropagation of Gentiana scabra Bunge which had been used the cut flower, pot flower ornamental and medicinal plants. Flower bud formation was affected by GA$_{3}$ and kinetin. The optimum concentrations for flower bud formation was observed at 0.5 mg/l kinetin and GA$_{3}$ , while kinetin was favorable. More flowerings result from the interaction of GA$_{3}$ and kinetin at in a combination of 0.1 mg/l kinetin + o.1 mg/l GA$_{3}$, but the optimum concentration of GA$_{3}$ and kinetin was decreased. All concentrations of kinetin with 0.1 mg/l GA$_{3}$ or O mg/l GA$_{3}$ + 0.5 mg/l kientin reduced t (weeks needed for 50% plantlets). The plantlet growth was affected by GA$_{3}$ and kinetin during plantlet culture. More lateral shots and better shoot length per plantlet were obtained as GA$_{3}$ and kinetin concentration were increased up to 1.0 mg/l. The number of per plantlet was greater increased in MS medium containing GA$_{3}$ than kinetin. Interaction was exhibited at lower concentration with 0.5mg/l GA$_{3}$ and kinetin, but not in higher concentration with 1.0 mg/l GA$_{3}$ and kinetin. Higher pod diameter increased seed germination, while lower pod diameter was obtained from abnormal plantlet. MA medium containing 0.5 mg/l GA$_{3}$ significantly increased germination without regard to pod diameter.

용담종자의 성숙정도와 저장방법이 발아력과 수명에 미치는 영향

손병구(Son Beung-Gu),최영환(Choi Young-Whan),안종길(Ahn Chong-Kil),조동(Cho Dong),강점순(Kang Jum-Soon),권오창(Kwon Oh Chang) 한국원예학회HST 1998 원예과학기술지 Vol.16 No.1

성숙정도, 저장조건 및 생장조절제 처리가 용담종자의 발아 및 수명에 미치는 영향

손병구(Son Beung Gu),최영환(Choi Young Whan),안종길(Ahn Chong Kil),조동(Cho Dong),강점순(Kang Jum Soon),권오창(Kwon Oh Chang) 한국원예학회 1999 Horticulture, Environment, and Biotechnology Vol.40 No.1

Viola속 식물의 원형질체 및 융합세포의 전자현미경 관찰

정용모,임현희,손병구,서정해,정정한,권오창,Chung, Yong-Mo,Im, Hyun-Hee,Son, Beung-Gu,Suh, Jung-Hae,Chung, Chung-Han,Kwon, Oh-Chang 한국생명과학회 1997 생명과학회지 Vol.7 No.4

To obtain a basic information on the development of Genus Viola, ultrastructure and electrofusion process between the two protoplasts from wild Viola callus cells and pansy mesophyll cells were observed with a scanning electron microscopy(SEM) and transmission electron microscopy(TEM). In the ultrastructural observation of wild viola callus protoplasts and pansy mesophyll protoplasts using SEM, their cell walls were removed completely. A knob-like formation was observed on the enlarge surface of viola callus protoplasts. On the surface of pansy mesophyll protoplasts net-like chloroplasts were observed. In SEM observation of pansy mesophyll protoplasts, chloroplasts devoid of membrane were observed on the surface the protoplasts. Pearl chain was formed by applying AC field of 200 V/cm at 1.0 MHz for 43 sec. The lysis of plasma membranes and fusion process occurred by applying a 1,600 V/cm DC pulse twice for 1 sec. After 1-2 hours of a DC pulse application, it was observed that the two protoplasts were fused completely into one cell. In TEM observation of the fused cell, many small vacuoles were located in the fusion area of the two protoplasts. Indeed, two distinct regions were observed during fusing process; in one region, a nucleus was found, while in the other region, both nucleus and nucleous were found.

Priming 처리에 의한 토마토 종자의 발아력과 Membrane Integrity에 미치는 영향

강점순(Kang Jum Soon),손병구(Beung Gu Son),안종길(Chong Kil Ahn) (사)한국생물환경조절학회 2003 생물환경조절학회지 Vol.12 No.4

당근 펠렛종자의 건조방법 및 저장조건이 발아성에 미치는 영향

강점순(Jum Soon Kang),손병구(Beung Gu Son),최영환(Young Whan Choi),이용재(Yong Jae Lee),주우홍(Woo Hong Joo),임채신(Chae Shin Lim),박영훈(Young Hoon Park) 한국생명과학회 2009 생명과학회지 Vol.19 No.4

당근 펠렛종자의 적정 건조조건과 종자활력을 유지시킬 수 있는 저장조건을 구명하기 위하여 일련의 실험을 수행하였다. 펠렛전 피복재료의 함수율은 4.9% (FW basis)였으나, 펠렛을 제조한 후에는 접착제를 흡수하여 함수율이 24%로 증가되었다. 이들 종자를 25℃, 35℃ 및 45℃에서 건조시키면 3시간 이후에 펠렛전의 함수율과 동일한 5% 수준으로 건조되었다. 무처리 종자와 펠렛종자간 발아율에는 큰 차이가 없었으며, 펠렛 후 건조온도와 기간에 따른 발아율에도 큰 차이가 없었다. 그러나 펠렛종자는 무펠렛 종자에 비해 T50은 약 3일 정도 지연되었다. 펠렛종자를 고온인 45℃에서 건조시간을 연장시키면 동일기간의 25℃나 35℃의 건조에 비해 발아속도가 지연되었다. 산업화를 위한 대량 건조시에 적용될 수 있는 적정 건조조건은 35oC에서 3시간 건조였다. 펠렛종자를 실온 저장하면 종자수명이 저하되었으나, 5℃ 저장은 퇴화속도가 완만하였다. 펠렛종자의 활력을 유지시킬 수 있는 적정 저장온도는 5℃였다. 펠렛종자를 실온에서 4개월 저장하면 저장 전에 비해 묘출현율이 14% 감소되었다. 그러나 5℃에 저장된 펠렛종자는 묘출현율 감소는 6%에 불과하였다. 5℃에 저장된 펠렛종자는 무처리에 비해 E50이 2일 정도 지연되었으나, 35일 생육 후의 건물중은 무처리와 큰 차이가 없다. This study was conducted to identify the optimum condition for dehydration and storage for maintaining the seed vigor in pelleted carrot seeds. The water content of solid materials of un-pelleted seeds was 4.9% (F.W. basis), and that of pelleted seeds was 24%. When we dehydrated pelleted seeds for 3 hr at 25℃, 35℃, and 45℃, all seeds were dehydrated to 5% - the same level of water content as that before pelleting. Pelleted seeds did not show any significant difference in germination from un-pelleted seeds, and neither was there any significant time or germination difference between pelleted and un-pelleted seeds at the tested dehydration temperatures. However, the T50 value of pelleted seeds increased about 3 days larger than that of un-pelleted seeds. Germination speed of pelleted seeds in which the dehydration period was prolonged at 45℃ was delayed compared to those prolonged at 25℃ or 35℃ for the same period. The optimum dehydration condition, which could be applied for large scale in the industry, was dehydration at 35℃ for 3 hr, and the optimum storage temperature which could maintain the seed performance was 5℃.

소형 다화 분지성 호접란 ‘SM 333’ 육성

박노은(No Eun Park),손병구(Beung Gu Son),김홍열(Hong Yul Kim),임기병(Ki-Byung Lim) 한국원예학회HST 2015 원예과학기술지 Vol.33 No.1

Priming 처리가 한지형 잔디류의 발아 증진에 미치는 영향

강점순(Jum Soon Kang),손병구(Beung Gu Son),최영환(Young Whan Choi),이용재(Yong Jae Lee),김용철(Young Chul Kim),최인수(In Soo Choi),주우홍(Woo Hong Joo),박영훈(Young Hoon Park) 한국생명과학회 2008 생명과학회지 Vol.18 No.8

Kentucky bluegrass와 Tall fescue에서는 발아력을 극대화시킬 수 있는 priming 최적 처리 조건은 100mM의 K₃PO₄ 용액으로 25℃에서 1일간 처리였다. Priming 처리된 종자는 무처리에 비해 높은 발아율을 보였고, T50과 MDG가 단축되어 신속한 발아를 유도하였다. 반면 NaOH나 KOH는 처리농도가 높아지고 처리기간이 길어질수록 발아력이 급격하게 저하되어 priming 화학제로는 적당하지 않았다. Creeping bentgrass 및 Bentgrass에서는 priming 처리에 관계없이 발아율이 10% 미만으로 낮았다. 그러나 200mM의 KNO₃ 로 3일간 priming 처리하면 발아율을 6%까지 향상시킬 수 있었으나, 현저한 수준은 아니었다. Kentucky bluegrass와 Tall fescue에서 priming 처리 기간은 1일 처리가 발아율 향상에 가장 좋았고, 처리 기간이 6일로 길어질수록 발아율이 저하되었고 발아 속도는 지연되었다. 또한 priming 화학제의 처리 농도는 100 mM 처리가 발아력 증진에 좋았으나, 200 mM과 300 mM로 처리농도가 높아지면 발아율이 감소하였다. This study was carried out to identify the optimum condition for priming, as a series of experiments for the enhancement of germination percentage and germination synchronization in cool-season turfgrass seeds. The optimum priming conditions to maximize the germinability in both Kentucky bluegrass and Tall fescue was a 1 day treatment of 100 mM K₃PO₄ at 25℃. The seeds treated with the optimum priming condition enhanced the germinability compared to control, and shortened the time for germination with reduced T50 and mean daily germination (MDG). However, as the concentration and treatment period of NaOH or KOH increased, significantly decrease in germination percentage was observed. Germination percentage in Creeping bentgrass and Bentgrass was below 10%, regardless of priming treatment. However, the priming treatment with 200 mM KNO₃for three days improved the germination up to 6%, which was not a statistically significant level. The most effective priming period for Kentucky bluegrass and Tall fescue was one day treatment, but the germinability was suppressed as the treatment period was extended until six days. For priming chemical treatment, germinability was improved with the concentration of 100 mM, while it was reduced at higher concentrations of 200 mM and 300 mM.

펠렛 피복물질의 물리, 화학적 특성이 당근 펠렛종자의 발아력에 미치는 영향

강점순(Jum Soon Kang),손병구(Beung Gu Son),최영환(Young Whan Choi),이용재(Yong Jae Lee),박영훈(Young Hoon Park),최인수(In Soo Choi) 한국생명과학회 2007 생명과학회지 Vol.17 No.12

펠렛의 목적은 기계화 정밀파종하여 파종과 솎음노력을 절감하는데 있다. 펠렛 피복물질의 용적밀도는 dialite, kaolin 및 talc 등이 낮았고 기공성은 높았다. 보수력이 우수한 피복물질은 bentonite와 dialite 이었으며, 184% 및 173%의 수분을 보유할 수 있었다. 반면 calcium carbonate, calcium oxide, fly ash등은 보수력이 낮은 펠렛 피복물질이었다. 펠렛 피복물질의 pH는 kaolin과 dialite에서 각각 6.8 및 7.4로 중성이었으나, limestone, calcium oxide, bentonite 등은 pH가 12.8, 13 및 10으로 강알카리였다. 전기전도도는 강알카리인 limestone, calcium oxide에서 높았다. 이와 같이 높은 pH와 전기전도도를 보인 피복물질들은 당근종자의 펠렛에 적합하지 않았다. 펠렛 피복물질를 EDS로 분석한 결과, Talc는 주성분이 Si (71%)이었고, Mg도 29% 함유하였다. 반면 calcium carbonate의 주요성분은 Ca (66.6%)이었으며, 이외에 Si (22.9%)와 Mg (10.5%)를 함유하였다. 펠렛 형성정도는 kaoline, talc 및 talc + calcium carbonate 혼합재료에서 우수하였다. 펠렛종자의 경도는 bentonite로 펠렛된 종자에서 가장 높았다. 수분흡수 후 펠렛층의 분해형태는 talc, limestone, zeolite, 및 fly ash는 열개형이었고, 용해형은 calcium carbonate와 calcium oxide등 이었다. 반면 bentonite와 vermiculite는 팽창형이었다. 수분흡수 후 펠렛층의 분해는 calcium carbonate 및 kaolin으로 펠렛된 종자에서 분해가 가장 빨랐다. 펠렛배율이 높아지면 발아속도(T50)는 지연되었다. 당근종자에서 적정 펠렛배율은 19배가 좋았다. Seed pelleting is generally conducted in order to save the labor for sowing and thinning by enabling the precision mechanical planting. In the present study, the influence of physical and chemical properties of pelleting solid materials was investigated on carrot seed germination. Among the pelleting solid materials evaluated, dialite, kaolin, and talc showed low bulk density and high porosity. Bentonite and dialite carried high water holding capacities of 184% and 173%, respectively, while calcium carbonate, calcium oxide, and fly ash showed relatively low water holding capacity. The pH of kaolin (6.8) and dialite (7.4) were close to neutral, while limestone (12.8), calcium oxide (13.0), and bentonite (10.0) were highly basic. High electro-conductivity was shown in limestone and calcium oxide. EDS analysis revealed that the main elemental compositions of talc were Si (71.0%) and Mg (29.0%), and those of calcium carbonate were Ca (66.6%), Si (22.9%), and Mg (10.5%). High granulation capacity was observed from talc and the mixture of talc and calcium carbonate. Seeds pelleted with bentonite showed the highest hardness. The dissolving type of the pellet layer after imbibition was split type in talc, limestone, zeolite, and fly ash, melt type in calcium carbonate and calcium oxide, and swell type in bentonite and vermiculite. The shortest dissolving time of pellet layer was observed from calcium carbonate and kaolin. The germination speed (T50) was delayed as the size of pelleted seeds increased. The optimum size of pelleting was 19 ratio in carrot.

국내 육성 거베라의 우량묘 생산에 미치는 식물생장조절제의 영향

정용모,신향정,박소영,안동춘,손병구,조용조,권오창,Chung, Yong-Mo,Sin, Hyang-Jung,Park, So-Young,An, Dong-Chun,Son, Beung-Gu,Jo, Yong-Cho,Kwon, Oh-Chang 한국생명과학회 2007 생명과학회지 Vol.17 No.6

This study was carried out to obtain information about the valuable combination and concentration to produce good plants of cut gerbera flowers of 19 domestically - bred gerbera cultivars. BA and kinetin combination is more effective than NAA and BA combination to produce good plants and mass propagation of domestically - bredgerbera cultivars. Normally almost of 19 cultivars, mass propagation was more effective on the medium containing 1.0 mg/L BA + 0.5, 1.0 mg/L kinetin. But some cultivars, 'Sunnyeo', 'Oksaem','Piny', and 'Pink Light', vitrified plants were induced on MS medium high level of BA concentration(BA 1.0 mg/L), in comparison with those on the medium with low level of BA(0.1 mg/L).Fresh and dry weight, more effective on the medium containing BA 0.5 mg/L . Kinetin 0.1, 0.5 mg/L. Anatomical investigation of vitrified leaf, stomata of vitrified leaves were circular and inflated, where-as those of normal leaves.