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Bae, Woo-Chul,Lee, Han-Ki,Choe, Young-Chool,Jahng, Deok-Jin,Lee, Sang-Hee,Kim, Sang-Jin,Lee, Jung-Hyun,Jeong, Byeong-Chul The Microbiological Society of Korea 2005 The journal of microbiology Vol.43 No.1
A soluble Cr(VI) reductase was purified from the cytoplasm of Escherichia coli ATCC 33456. The molecular mass was estimated to be 84 and 42 kDa by gel filtration and SDS-polyacrylamide gel electrophoresis, respectively, indicating a dimeric structure. The pI was 4.66, and optimal enzyme activity was obtained at pH 6.5 and $37^{\circ}C$. The most stable condition existed at pH 7.0. The purified enzyme used both NADPH and NADH as electron donors for Cr(VI) reduction, while NADPH was the better, conferring 61% higher activity than NADH. The $K_m$ values for NADPH and NADH were determined to be 47.5 and 17.2 umol, and the $V_max$ values 322.2 and 130.7 umol Cr(VI) $min^{-1}mg^{-1}$ protein, respectively. The activity was strongly inhibited by N-ethylmalemide, $Ag^{2+},\;Cd^{2+},\;Hg^{2+}$, and $Zn^{2+}$. The antibody against the enzyme showed no immunological cross reaction with those of other Cr(VI) reducing strains.
Bae, Hong-Chul,Hong, Seri,Jang, Sung-In,Lee, Kwang-Sig,Park, Eun-Cheol The Korean Society for Preventive Medicine 2015 예방의학회지 Vol.48 No.3
Objectives: The purpose of this study was to investigate the association between suicidal behavior and patterns of alcohol consumption in Korean adults. Methods: This study was based on data provided by the Korea National Health and Nutritional Examination Survey from 2007 to 2011. A total of 42 347 subjects were included in the study, of whom 19 292 were male and 23 055 were female. Logistic regression analysis was performed to assess the association between patterns of alcohol consumption and suicidal behavior. Results: Among the study subjects, 1426 males (11.3%) and 3599 females (21.2%) had experienced suicidal ideation, and 106 males (0.8%) and 190 females (1.1%) had attempted suicide during the previous 12 months. Alcohol Use Disorders Identification Test (AU-DIT) scores were found to be associated with suicidal ideation in males and associated with both suicidal ideation and suicide attempts in females. Alcoholic blackouts were associated with suicidal ideation and suicide attempts in males, and were also associated with suicidal ideation in females. Conclusions: In this study, we found that certain patterns of alcohol consumption were associated with suicidal behaviors. In particular, only alcoholic blackouts and categorized AUDIT scores were found to be associated with suicidal behavior in males. We therefore suggest that further research is needed to examine this relationship prospectively and in other settings.
Mitochondrial DNA Sequence Variation of the Firefly , Pyrocoelia rufa ( Coleoptera : Lampyridae )
(Sang Chul Lee),(Ik Soo Kim),(Jin Sik Bae),(Byung Rae Jin),(Sam Eun Kim),(Jong Kil Kim),(Hyung Joo Yoon),(Sung Ryul Yang),(Soo Ho Lim),(Hung Dae Sohn) 한국잠사학회 2000 한국잠사학회 잠사과학 100주년 국제심포지엄 및 추계학술대회 Vol.- No.-
( Bae Hwan Kim ),( Woo Chan Son ),( Cha Ok Yim ),( Sung Keun Kang ),( Jeong Chan Ra ),( Young Chul Kim ) 한국조직공학과 재생의학회 2010 조직공학과 재생의학 Vol.7 No.5
The potential anti-wrinkle effects of adipose tissue-derived mesenchymal stem cells (AdMSCs) have recently been reported. In the present study, we demonstrated a protective effect of AdMSCs in a UV irradiationinduced hairless mouse wrinkle model. Mice treated with AdMSCs showed improvements in skin erythema index, moisture capacity and transepidermal water loss. The AdMSC-treated mice showed reduced wrinkle area, as measured by gross observation and replica image analysis, and had a thinner epidermis and increased collagen and elastic fiber content, compared with the control group, as measured by histopathological examination. These results suggested that AdMSCs may be effective in the treatment of wrinkles. The activity of AdMSCs was further confirmed by the reduction in MMP-3 mRNA expression in the skin of the AdMSC treated animals, which suggests that AdMSCs can protect the collagen from degradation induced by UV irradiation. Further studies will investigate detailed mechanisms of action of AdMSCs, with particular focus on AdMSC-derived secretory factors.
( Sung Woo Cho ),( Jung Hoon Cha ),( Na Ri Park ),( Won Hee Hur ),( Pill Soo Sung ),( Jong Young Choi ),( Seung Kew Yoon ),( Si Hyun Bae ),( Hee Chul Nam ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1
Aims: Non-alcoholic fatty liver disease (NAFLD) is a metabolic- related disorder ranging from simple steatosis to more severe forms, but the exact mechanism of progression remains unknown. MicroRNAs(miR), a class of small noncoding RNAs, are implicated in controlling a variety of biological processes. The aim of this study is to investigate the regulatory and protective role of miR-22-3p in NAFLD progression. Methods: Both in vitro and in vivo models of NAFLD were generated by treating HepG2 and Huh-7 cells with palmitic acid (PA) and by feeding mice a high-fat diet (HFD), respectively. HE and Oil Red O staining were used to examine liver tissue morphology and lipid deposition, respectively. qRT-PCR (quantitative real time polymerase chain reaction) was used for investigate expression of miR, SIRT1, and proteins involved in lipogenesis Results: HFD-mice hepatic tissues and PA-treated HepG2 and Huh-7 cells presented excess lipid production. Both in vitro and in vivo NAFLD model displayed decreased miR-22-3p and SIRT1 expression as evidenced by qRT-PCR. Overexpression of miR-22-3p induced downregulation of FAS, PPAR gamma and SREBP-1c via upregulation of SIRT1 expression. Reduction of hepatic lipid accumulation was observed by Oil red O staining. Conclusions: In this study, miR-22-3p had a role in ameliorating hepatic lipogenesis by regulation of SIRT1 signal pathway in NAFLD model. The overexpressed miR-22-3p protects hepatocytes from lipid metabolism and suppresses hepatic lipogenesis, suggesting as a potential target for the therapeutic strategy of NAFLD.