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최치은 고요아침 2016 한국동시조 Vol.- No.2

국내 홀스타인젖소의 선형심사에 대한 보정계수 개발

송치은,상병찬,도창희 한국동물자원과학회 2002 한국축산학회지 Vol.44 No.1

젖소의 선형심사형질은 젖소의 생물학적 변이를 심사자의 직관에 의하여 변이의 정도에 따라 점수를 부여한다. 심사형질에 대한 정의에 의하여 연령과 비유기에 상관없이 변이의 정도를 수치화 하기 때문에 형질에 따라서 필연적으로 연령과 비유기의 영향을 받을 수밖에 없다. 본 연구에서는 연령과 비유기가 선형심사형질에 미치는 영향을 조사하고 그를 보정하기 위한 보정계수를 추정하였다. 키 강건성, 체심, 엉덩이 너비, 뒷다리 옆모양과 앞유방 부착이 연령에 따라 증가 또는 감소하는 경향을 보이면서 크게 영향 받는 것으로 조사되었으며, 유용성, 뒷유방 높이, 뒷유방 너비와 정중제인대는 비유기에 따라 영향을 받았다. 유방깊이는 연령과 비유기 두 가지 요인 모두로부터 영향을 받았다. 추정된 보정 계수에 의한 각각의 보정계수를 적용한 결과 연령과 비유기에 영향 받는 모든 형질에서 분만년월, 심사자 등의 변이에는 작은 영향을 미치며, 보정하고자 하는 요인의 변이를 크게 줄여 보정효과가 양호한 것으로 나타났다. 유방깊이는 연령과 비유기에 대한 보정계수를 동시에 적용한 결과 두 요인 모두에서 변이를 크게 줄였다. The environmental effects were studied to estimate age and lactation stage adjustment coefficients in the primary linear traits of Holstein dairy cattle in Korea. Calving year month, classifier, age-month at classification and lactation stage were the environmental factors which significantly affected the most of linear traits at the level of 0.01. F values of Stature, strength, body depth, thurl with and rear leg side view were relatively higher in the effects of age-month, and dairy form, rear udder height, rear udder width, and udder cleft had relatively higher F values in the effects of lactation stage. Udder depth were affected highly by both age-month and lactation stage. Through the least square means of traits and the interpolation and smoothing obtained by the regression analysis of log and exponential transformed adjustment coefficients, age-month and lactation stage coefficients were estimated, and applied to real data to check the variation in the age-month and lactation stage effects. The estimated mean squares showed that the variation in all the linear traits significantly were decreased for the adjusted factors without the significant changes of variation in calving year month and classifier. That udder depth adjusted for both the age-month and lactation stage resulted in the decreases of variation in the both effects.

市販 「黃精」의 剖見

李致殷,沈宗輔,金良武 성균관대학교 1964 成均藥學 Vol.5 No.-

Botanical origin of WIIAKG-JOUNG on the market In Seoul are Polygonalum japonicum, Polygonatum sibiricum, and Polygonatum inflactum. We can get thedifferent result of Higashi, Japanese Pharrnacognogist, theory about WHANG-JOUNG.that are the existence of endodermis and the structure of the Vascular Bundle of central part

연령에 따른 팩 구매요인 및 구매 방법에 관한 연구

송치은,임연실,전해정 한국웰니스학회 2019 한국웰니스학회지 Vol.14 No.3

본 연구의 목적은 연령에 따른 팩 구매요인 및 구매방법을 알아 보기 위함이다. 연구의 대상자는 서울, 수도권에 거주하는 20대∼50대 여성들이며 2018년 4월 11일부터 5월 4일까지 진행하였고, 설문지와 모바일을 통하여 총 397부를 회수하여 설문조사하였다. 자료 분석으로는 빈도분석과, 카이스케어 검정, 일원변량분석으로 통계 처리하였고 실증분석은 SPSSWIN 21.프로그램으로 분석하였다. 본 연구에서 나타난 결과로는 팩 인식은 40대, 20대, 30대 순으로 인식이 높았고, 연령에 따른 팩 구매의 요인은 모든 연령별로 유의미하게 차이가 있었다. 또한 연령에 따른 본인이 선호하는 팩의 제형 타입으로 20대, 30대, 40대 이상 모든 연령에서 ‘시트 타입’이 가장 높게 나타났으며 팩 사용목적은 20대와 30대, 40대 이상 모두 ‘피부개선’에서 가장 높게 나타났다. 이와 같은 결과는 연령에 따른 팩 인식과 구매요인에 따른 팩의 제품개발을 해야 하다는 것을 시사한다. The purpose of this study is to find out the factors and methods of purchasing packs according to age. The subjects of the study were women in their 20s and 50s living in Seoul, the capital area, which lasted from April 11 to May 4, 2018. This study was conducted by retrieving a total of 397 copies through questionnaire and mobile using the survey method. The data analysis was statistically analyzed by frequency analysis department, Chi-square test and one-way variation analysis, and the empirical analysis was analyzed by SPSSWIN 21.0 program. This study was conducted by retrieving a total of 397 copies through questionnaire and mobile using the survey method. As a result of this study, the recognition of pack recognition was high in the order of people in their 40s, 20s and 30s, and the factors of purchasing pack with age varied significantly across all ages. In addition, 'seat type' was the highest among all ages, as the type of pack preferred by age, while 'skin improvement' was the highest among those in their 20s, 30s and 40s and older. These results are meaningful in that they presented product development based on research on how to apply packs based on age-based recognition of packs and the results of purchasing factors.

朝鮮小作改善運動(下) : 先づ小作法を設くるを要す

孫致殷 朝鮮思想通信社 1929 朝鮮思想通信 Vol.- No.843

Diversity and antifungal activity of endophytic bacteria associated with Panax ginseng seedlings

홍치은,조성희,조익현,박정미 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.6

Ginseng (Panax ginseng C.A. Meyer) is a medicinal crop that requires a long culture time before it is ready to harvest, thus generating high economic and environmental costs. Symbiotic bacteria that live within the plant provide the host plant with many advantages in terms of metabolism and disease resistance. Here, we isolated endophytic bacteria from various tissues of P. ginseng seedlings using a culture-dependent method and we compared their tissue distribution. In addition, their antimicrobial activity against two fungal pathogens was investigated. Based on 16S rRNA sequencing, we identified 21 bacterial strains from ginseng seedlings. Leaves and rhizomes showed higher bacterial species diversity than root bodies and tails. While Bacillus strains were detected in all tissues, Xanthomonas and Micrococcaceae strains were specifically isolated from rhizome and leaf tissues, respectively. Fourteen bacterial strains showed antimicrobial activities against Cylindrocarpon destructans and/or Botrytis cinerea, with different activities. Among them, two strains (PgKB29 and PgKB35) showed strong antimicrobial activities against both fungi. Taken together, these results provide a better understanding of endophytic bacteria in P. ginseng seedlings and suggest the possibility of biological control of fungal pathogens using endophytic bacteria.

치과용 인상재에 따른 모형재간의 적합정밀도에 관한 비교연구

최치은(Chi Eun Choi),이호용(Ho Yong Lee) 대한치과의사협회 1983 대한치과의사협회지 Vol.21 No.2

The Arabidopsis Phytocystatin AtCYS5 Enhances Seed Germination and Seedling Growth under Heat Stress Conditions

송치은,김태윤,정우식,임채오 한국분자세포생물학회 2017 Molecules and cells Vol.40 No.8

Phytocystatins (PhyCYSs) are plant-specific proteinaceous inhibitors that are implicated in protein turnover and stress responses. Here, we characterized a PhyCYS from Arabidopsis thaliana, which was designated AtCYS5. RT-qPCR analysis showed that the expression of AtCYS5 in germinating seeds was induced by heat stress (HS) and exogenous abscisic acid (ABA) treatment. Analysis of the expression of the β-glucuronidase reporter gene under the control of the AtCYS5 promoter showed that AtCYS5 expression during seed germination was induced by HS and ABA. Constitutive overexpression of AtCYS5 driven by the cauliflower mosaic virus 35S promoter led to enhanced HS tolerance in transgenic Arabidopsis, which was characterized by higher fresh weight and root length compared to wild-type (WT) and knockout (cys5) plants grown under HS conditions. The HS tolerance of AtCYS5-overexpressing transgenic plants was associated with increased insensitivity to exogenous ABA during both seed germination and post-germination compared to WT and cys5. Although no HS elements were identified in the 5’-flanking region of AtCYS5, canonical ABA-responsive elements (ABREs) were detected. AtCYS5 was upregulated in ABA-treated protoplasts transiently co-expressing this gene and genes encoding bZIP ABRE-binding factors (ABFs and AREB3). In the absence of ABA, ABF1 and ABF3 directly bound to the ABREs in the AtCYS5 promoter, which activated the transcription of this gene in the presence of ABA. These results suggest that an ABA-dependent pathway plays a positive role in the HS-responsive expression of AtCYS5 during seed germination and post-germination growth.

Kinesin family member KIF18A is a critical cellular factor that regulates the differentiation and activation of dendritic cells

김세영,조용빈,송치은,윤성일,서영진 한국유전학회 2020 Genes & Genomics Vol.42 No.1

Background KIF18A is a kinesin family member that is involved in various cellular processes including cell division, cell transformation, and carcinogenesis. However, its possible role in the regulation of host immunity has not been examined. Objective The aim of this study is to investigate the functional role of KIF18A in the differentiation and activation of dendritic cells (DCs) that are the most efficient antigen-presenting cells. Methods A bioinformatic analysis of the KIF18A gene family was performed to understand its sequence variability and evolutionary history. To inhibit KIF18A activity, a highly specific small molecule inhibitor for KIF18A, BTB-1 was used. DCs were differentiated from mouse bone marrow (BM) cells from 6 to 7 week old C57BL/6 mice with recombinant granulocyte–macrophage colony-stimulating factor (GM-CSF). Expression of KIF18A was measured by Western blotting. The surface expression of differentiation and activation markers on DCs were analyzed by flow cytometry. Results The phylogenetic analysis revealed that the KIF18A gene family is remarkably conserved across vertebrates. Interestingly, the expression of KIF18A was increased as BM precursor cells differentiated into DCs. BTB-1 treatment strongly inhibited the differentiation of BM cells into DCs in a dose-dependent manner. Furthermore, treatment of immature DCs with BTB-1 significantly impaired the expression of activation markers on DCs including MHC class I, CD80, and CD86 upon TLR4 or TLR7 treatment. Conclusion Our results reveal that KIF18A is a critical DC differentiation and activation regulator. Therefore, KIF18A could be a potential therapeutic target for immune-mediated disorders.

의료기관 간호사에 대한 수혈관련 교육 시행 효과 분석

임진숙,최새롬,송치은,박유미,임영애,김준년,권계철 대한수혈학회 2020 大韓輸血學會誌 Vol.31 No.3

Background: Nurses play a central role in the process of blood transfusion because they have the closest interactions with patients. Providing nurses with the appropriate knowledge and competency through education can help ensure transfusion safety. This study investigated the effectiveness of the first nationwide transfusion-related education for nurses by analyzing questionnaires for a self-assessment of competency on transfusion-related knowledge before and after the education and general evaluation for the educational program. Methods: The education program was composed of four lectures and was conducted in seven regions in South Korea. One hundred and ninety-two nurses participated, and the questionnaires for 170 nurses were analyzed. Results: The participants consisted of 90 nurses (53.0%) from tertiary hospitals, 23 (13.5%) from general hospitals, and 57 (33.5%) from other hospitals. The majority of the participants (103/170, 60.6%) were from hospitals with ≥500 beds, and 69.4% had a work period of ≥10 years. The scores for pre-/post-education self-assessment of competency were as follows: blood components, 3.03/3.73; pretransfusion testing, 2.86/3.64; management of transfusion, 3.18/3.84; and transfusion reactions, 3.11/3.78. In all categories, there was a significant increase in the score after the education program. The majority of participants (99.4%) provided a positive response regarding the necessity of a transfusion-related education program. Conclusion: South Korea’s first attempt at transfusion-related education for nurses showed a strong positive effect by improving participants’ transfusion-related competency. Considering the important role of nurses in blood transfusion, the educational program should continue and be expanded in the future. (Korean J Blood Transfus 2020;31:222-229)