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Ji Hoon Jeong,박기남,Joo Hyun Kim,KyungMu Noh,허성식,김윤혜,Moonju Hong,Jun Chul Chung,박재홍,이종순,Young-Ik Son,이주헌,김상헌,황용성 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00
Background Human omentum-derived mesenchymal stem cells (hO-MSCs) possess great potential to differentiate into multiple lineages and have self-renewal capacity, allowing them to be utilized as patient-specific cell-based therapeutics. Although the use of various stem cell-derived β-cells has been proposed as a novel approach for treating diabetes mellitus, developing an efficient method to establish highly functional β-cells remains challenging. Methods We aimed to develop a novel cell culture platform that utilizes a fibroblast growth factor 2 (FGF2)-immobilized matrix to regulate the adhesion and differentiation of hO-MSCs into insulin-producing β-cells via cell–matrix/ cell–cell interactions. In our study, we evaluated the in vitro differentiation potential of hO-MSCs cultured on an FGF2- immobilized matrix and a round-bottom plate (RBP). Further, the in vivo therapeutic efficacy of the β-cells transplanted into kidney capsules was evaluated using animal models with streptozotocin (STZ)-induced diabetes. Results Our findings demonstrated that cells cultured on an FGF2-immobilized matrix could self-organize into insulin- producing β-cell progenitors, as evident from the upregulation of pancreatic β-cell-specific markers (PDX-1, Insulin, and Glut-2). Moreover, we observed significant upregulation of heparan sulfate proteoglycan, gap junction proteins (Cx36 and Cx43), and cell adhesion molecules (E-cadherin and Ncam1) in cells cultured on the FGF2-immobilized matrix. In addition, in vivo transplantation of differentiated β-cells into animal models of STZ-induced diabetes revealed their survival and engraftment as well as glucose-sensitive production of insulin within the host microenvironment, at over 4 weeks after transplantation. Conclusions Our findings suggest that the FGF2-immobilized matrix can support initial cell adhesion, maturation, and glucose-stimulated insulin secretion within the host microenvironment. Such a cell culture platform can offer novel strategies to obtain functional pancreatic β-cells from patient-specific cell sources, ultimately enabling better treatment for diabetes mellitus.
Odelia Levana,Ji Hoon Jeong,Sung Sik Hur,Wonbin Seo,Minho Lee,Kyung Mu Noh,Soonkook Hong,Jae Hong Park,이주헌,Chulmin Choi,황용성 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.120 No.-
Biofilm formation on biomedical implant surfaces requires bacterial adhesion, which increases the risk ofinfection and chronic inflammation. Since intercalation of quaternary ammonium salts (QAS) into montmorillonite(MMT) clay, known as organoclays, has been reported to increase surface broad-spectrumantibacterial properties, we aimed to develop an antibacterial surface composed of thermoplastic polyurethane(TPU) embedded with bentonite and MMT clay containing QAS to prevent initial bacterialattachment. We evaluated its potential application in reducing bacterial adhesion and enhancingbacteria-killing properties using Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. Our results demonstrated that the nanoclay-embedded TPU surfaces with QAS significantly reduced theadhesion of E. coli and S. aureus by 68.82% and 65.18%, respectively, compared to the plain TPU surfaces. Additionally, a higher nanoclay concentration coating on the surface could enhance its effectiveness, asshown by 85.34% and 82.74% reduction in E. coli and S. aureus adhesion and killing efficiency. Furthermore, we observed that nanoclay-embedded TPU surfaces had no detrimental effects on the viabilityof human dermal fibroblasts. Taken together, these techniques could provide novel strategies forinhibiting bacterial adhesion and supporting bacteria killing on biomedical implant surfaces, as the investigatedsurfaces are simple to synthesize, efficient, and cost-effective.