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한국산 고등 균류의 성분 연구 (제33보) : 간버섯의 항암 성분 Antitumor Components of Trametes sanguinea
김병각,정경수,홍화봉,우명식 한국균학회 1982 韓國菌學會誌 Vol.10 No.4
To find antitumor components with low toxicity from natural resources, antitumor test of the water extract of the carpophores of Trumetes sanguinea (L. ex Fr.)Lloyd. was undertaken. The carpophores of this fungus were collected in Gyeong Gi Province and extracted with hot water. The extract was purified by dialyzing through Visking tube and a protein-bound polysaccharide fraction was obtained. The fraction was tested for antitumor activity against sarcoma 180 implanted in mice. The tumor inhibition ratio of the fraction against the tumor was 72.4% at the dose of 10㎎/㎏/day for the period of ten days. The tumor in one of the eight mice was completely regressed. The antitumor components were found to be a polysaccharide and a protein. The hydrolysis of the polysaccharide moiety yielded three monosaccharides, and from the hydrolysate of the protein moiety 13 amino acids were identified.
13. 한국산 고등균류의 성분연구 (33) : 간버섯의 항암성분 Antitumor Components of Trametes sanguinea (L. ex Fr.) Lloyd .
최응칠,정경수,강창율,홍화봉,김병각 한국균학회 1982 韓國菌學會誌 Vol.10 No.1
For the purpose of finding out the stronger antitumor compounds with the lower toxicity from the natural resources, the antitumor activity test of the water extract of the carpophores of Trametes sanguinea (L. ex Fr.) Lloyd, was undertaken. The carpophores of T. sanguinea, a high fungi, were collected in Gyeong Gi Province and extracted with hot water. The extract was purified by dialyzing through Visking tube and a polysaccharide fraction was obtained. The antitumor activity of the fraction was tested against sarcoma 180 implanted in mice. The tumor inhibition ratio of the polysaccharide fraction against the tumor was 72.4% in the doses of 10㎎/㎏/day for the period of ten days. The tumor in two of the 8 mice was completely regressed. The components of this aqueous extract were found to be a polysaccharide and a protein by color reactions including Anthrone and Lowry-Folin tests. The chemical analysis of the polysaccharide fraction was also undertaken.
12. 한국산 고등균류의 성분에 관한 연구 (34) : 붉은 싸리버섯의 항암 성분 Antitumor Components of Ramaria formosa (Fr.) Quel
김병각,최응칠,이경림,홍화봉,유인숙 한국균학회 1982 韓國菌學會誌 Vol.10 No.1
To investigate the antitumor components of Korean higher fungi, the carpophores of Ramaria formosa (Fr.) Que´l were collected in Gang Won Province and extracted with hot water. The extracts were concentrated and precipitated by four volumes of enthanol. The precipitates were centrifugated and purified lay dialyzing through visking tube and polysaccharide fractions were obtained. The antitumor activity of the fraction was tested against Sarcoma 180 implanted in mice. The tumor inhibition ratio of the polysaccharide fraction against the tumor was 66% in the dose of 50㎎/㎏/day for the period of tert days. The tumor in two of the ten mice was completely regressed. The components of these aqueous extracts were found to be polysaccharide and protein by color reactions including Anthrone and Lowry-Folin tests. The chemical analysis of the polysaccharide fraction was also undertaken.
김병각,한대석,양문식,홍화봉 한국균학회 1980 韓國菌學會誌 Vol.8 No.2
To investigate constituents of Lampteromyces japonicus (Kawam.) Singer which grows wildly in Korea, the carpophores of the fungus were collected in Gyeong Gi Province and analyzed for sterols and amino acids by gas chromatography and an amino acid autoanalyzer. The results showed that it contained stigmasterol and ergosterol and that it contained eleven free amino acids. These free amino acids were also determined quantitatively. Furthermore, sixteen amino acids were identified in the total amino acid fraction of the fruiting body.