Monochloroacetaldehyde의 합성에 관한 연구

홍성엽 서경대학교 산업기술연구소 2000 産業技術硏究所論文集 Vol.8 No.-

Monochloroacetaldehyde is a very useful compound as a raw material for the synthesis of medicinal and agricultural chemicals. Because of the unstability of the monochloroacetaldehyde, many new synthetic method for the compound were proposed such as the chloroacetaldehyde's concentration in water and chloroacetaldehyde trimer. In this report, some new factors were proposed to improve the yield to synthesize and the purity of monochloroacetaldehyde. The synthetic method of monochloroacetaldehyde in organic solvent instead of in water have shown good result in the point of the yield and the purity.

Biodiesel 생산 과정의 부산물인 Glycerin의 활용

홍성엽 서경대학교 산업기술연구소 2010 産業技術硏究所論文集 Vol.24 No.-

Biodiesel is produced by transesterification reaction between vegetable oil and methanol with catalysts Glycerin is obtained from the transesterification reaction as a by-product. In this research, a glycerin ester and glycerin ether as derivatives of the glycerin were synthesized. The glycerin ester was obtained esterification reaction between glycerin and acetic anhydride.

급성흉통의 진단적 프로토콜에 대한 전향적 연구

홍성엽,김갑득,강명희 대한응급의학회 2003 대한응급의학회지 Vol.14 No.3

Purpose: For appropriate triage of chest pain patients in the emergency room (ER), We verified whether or not a new chest pain protocol, which is a combination of Goldman algorithm Cardiac STATus® adequately reflects the risk of a major cardiac event in patients with acute chest pain. Methods: All patients arriving at the ER were divided into 4 risk group based on the Goldman Ml probability and on the result of cardiac STATus®. one month later, we collected information on whether the patients had undergone major cardiac event or death. We analyzed relative risk (RR) of cardiac event by using a logistic regression and we com pare the event-free survival rates and survival lengths. RR=2 mean that the risk of cardiac events is high by two folds than control group. Results: There were 31 major cardiac events. the relative risk of major events of each group compared to group 4 were 40.1 for group 1, 51.9 for group 2 and 27.4 for group 3. Group 4 patients experienced one cardiac event and no deaths. The survival time without a cardiac event of group 4 patients approach 30 days within experimental error. The survival rate without a cardiac event is 0.98±0.02. Therefore, the patients in group 4 had life expectancies similar to those of normal persons. Conclusion: This chest pain protocol adequately reflects the chances of cardiac event, so patients who show a negative result in cardiac STATus® and a low Goldman MI probability can be safely discharged from ER and followed up in outpatient clinic.

Quantitative lateral-flow immunoassay for the assessment of the cartilage oligomeric matrix protein as a marker of osteoarthritis

홍성엽,박유민,장요한,민병현,윤현철 한국바이오칩학회 2012 BioChip Journal Vol.6 No.3

This study reports a simple and quantita-tive analytical method for detecting cartilage oligome-ric matrix protein(COMP) using a lateral-flow immu-noassay(LFI) format. An immuno-chromatographic assay was developed using a gold nanoparticle(AuNP)-conjugated monoclonal antibody(Ab) probe for the quantitative detection of COMP in human synovial fluid(SF). The detection antibody was conjugated with the AuNP(~30nm) to enable detection, and the cap-ture antibody was immobilized within a multi-spot pattern in the test zone of a membrane strip. COMP in the human SF binds to the AuNP-conjugated detection antibody, and the COMP-Ab complex then binds to the capture antibody. The immunoassay test can be analyzed by visualizing the test strip with a commer-cial scanner and an imaging software program. The color density of the multi-spot pattern at the test zone increased in proportion to the concentration of COMP. The developed immunoassay exhibited a dynamic de-tection range from 0.6 to 20μg/mL of COMP. The detection limit of the proposed LFI system was approx-imately 0.2μg/mL. The test results showed good cor-relation with those obtained using a conventional en-zyme-linked immunosorbent assay(ELISA) to detect COMP. We expect that the proposed immunoassay method, based on the quantification of COMP, is sui-table for osteoarthritis diagnosis using SF samples.

시부트라민의 합성 및 분석 방법에 대한 연구

홍성엽 서경대학교 산업기술연구소 2010 産業技術硏究所論文集 Vol.25 No.-

Sibutramine is originally known as an anti-obesity drug. The biological activities of sibutramine are various, such as depression, parkinson`s disease, diabetes mellitus, and obesity. Sibutramine maleate was synthesized from sibutramine free base which also synthesized by 4 steps reactions. The intermediates to synthesize sibutramine are 1-(4-chlorophenyl)-1-cycloisobutylcarbonitrile, 1-isobutyryl-1-(4-chlorophenyl)cyclobutane, and N-formyl-1-[1-(4- chlorophenyl)cyclobutyl]isobutylamine. The synthesized sibutramine maleaste are assayed by UV/visible spectroscopy and HPLC with UV/visible detector. The purity of the synthesized sibutramine maleate are determined from the HPLC data.

Designed Urease의 합성

홍성엽 서경대학교 산업기술연구소 2007 産業技術硏究所論文集 Vol.19 No.-

Urease is a enzyme which catalyzes the urea hydrolysis. The design urease is mimiced the urease. The structure of the designed urease is composed of macrocyle and Zn. The macrocycle compound is synthesized by the reaction between picolyl chloride and tetraazatetradecane. The designed urease is accomplished by the chelation of Zn2+ ion to the macrocycle compound.

The Current Status and Future Outlook of Quantum Dot-Based Biosensors for Plant Virus Detection

홍성엽,이철호 한국식물병리학회 2018 Plant Pathology Journal Vol.34 No.2

Enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR), widely used for the detection of plant viruses, are not easily performed, resulting in a demand for an innovative and more efficient diagnostic method. This paper summarizes the characteristics and research trends of biosensors focusing on the physicochemical properties of both interface elements and bioconjugates. In particular, the topological and photophysical properties of quantum dots (QDs) are discussed, along with QD-based biosensors and their practical applications. The QD-based Fluorescence Resonance Energy Transfer (FRET) genosensor, most widely used in the biomolecule detection fields, and QD-based nanosensor for Rev-RRE interaction assay are presented as examples. In recent years, QDbased biosensors have emerged as a new class of sensor and are expected to open opportunities in plant virus detection, but as yet there have been very few practical applications (Table 3). In this article, the details of those cases and their significance for the future of plant virus detection will be discussed.

암로디핀의 합성 및 분석법에 대한 고찰

홍성엽 서경대학교 산업기술연구소 2010 産業技術硏究所論文集 Vol.24 No.-

Amlodipine besylate is a hypotensive drug. Amlodipine besylate was synthesized from N-(2-hydroxyethyl)phthalimide by 3 steps. 4-(2-chlorophenyl)-3-ethoxycarbonyl-5-methoxycabonyl-6-methyl-2(2-phtalimide ethoxymethyl)-1, 4-dihydropyridine is a second intermediate for the amlodipine besylate. After amlodipine besylate was synthesized, the spectroscopic analysis of amlodipine besylate was underwent to take HPLC assay data.

A Study of Ferrofluid as a Convergent Material with Arts and Music

홍성엽 한국전시산업융합연구원 2018 한국과학예술융합학회 Vol.36 No.-

Nano science and nanotechnology has convergence with sports, music, and arts beyond convergence with other scientific technologies, and comes to develop into a totally new area. Ferrofluids feature the creation of the magnetic field induced shape on the ferrofluid surface, combination of drops, and magnetic microconvection. Because of its small particles size, a ferrofluid has been developed as a promising new nanomaterial the beginning. Ferrofluids are more applied to diverse areas, such as dynamic loudspeakers, computer hardware, dynamic sealing, and bioengineering because of their unique properties. The research method is as follows. Firstly, introduction of the background and scope of the study. The basic theories for ferrofluid science and examples of convergent work with ferrofluids are known and published. Secondly, the classification of magnetic fields are introduced. There are generally two type of magnetic fields which are magnetorheological fluids and ferrofluids. A ferrofluid is a colloidal suspension of magnetic particles with a very tiny size in a liquid carrier. A ferrofluid is different from MRF with regard to its fluidity even if subjected to strong magnetic fields. Thirdly, A key to the application of ferrofluids is colloidal stability. The stability is determined by the balance of multiple types of energy (attractive and repulsive forces) which are the factors to prevent separation for a given time after the disappearance of exterior magnetic fields and thermal energy. If average thermal energy exceeds a total of interaction, negligible agglutination arises. There are two types of ferrofluid which are water-based ionic fluid and organic media based ferrofluid. To use ferrofluid to convergent with arts or other areas, important factors are low viscosity, low vapor pressure and chemical inertness of carrier liquids and surfactants. Finally, There are many areas which convergent with ferrofluid to invent totally new work and to sell fantastical products which never meet before. Those areas are arts, music, sculpture, and commercial products. According to the development the nanotechnology, the convergence of ferrofluid with arts or other areas will be accelerate and develop further and intensively.

Analysis of Non-Universality of Golden Ratio Known as a Perfect Figurative Ratio

홍성엽,이철호 한국전시산업융합연구원 2015 한국과학예술융합학회 Vol.22 No.-

Golden ratio, defined as symbol φ (Phi) simply in the 20th century, was acknowledged as the most balanced beautiful ratio, so it has been used in architecture or pictures etc. Golden ratio is the significant figurative ratio deeply related to our actual lives but it is not explained why many people consider it cozy and stable even in today, technologically advanced. Some refutation that golden ratio is only mathematical theory but there are few cases to be applied to practice. First, it was examined that there are many cases to exaggerate a small number of coincidences with golden ratio by chance in spite of numerous ratios in the nature. Besides, there are many cases that proportions of rectangular objects such as name card, credit card or proportions such as HDTV and wide monitors etc are approximate values of golden ratio but it is hard to place under the category of golden ratio for there are big differences with golden ratio. Rather, it was confirmed that it is close to Geumgang ratio, Korean own figurative ratio. Geumgang ratio is also called harmonic proportion and has flexibility to fit well to the cultural atmosphere of modern society which respects diversity. In this point of view, it is considered the active promotion according to global age would be need on the basis of more in-depth study on Geumgang ratio, likewise examples of golden ratio in the Western world.