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정현도,허민도 ( Hyun Do Jeong,Min Do Huh ) 한국수산과학회 1995 한국수산과학회지 Vol.28 No.1
Defining the mechanisms of B cell diversification which establish the immune repertoire is fundamental to understand how the immune response is regulated. In this report, B cell differentiation and diversification focused on the regulation of immunoglobulin V_(H) gene expression during ontogeny were analyzed by in situ hybridization technique. Fetal liver B cells in different gestational days from 16d to 20d showed the predominant expression of V_(H)7183 and V_(H)Q52 without transition of repertoire during the observed gestation days. The two subsets of fetal liver B cells separated according to different differentiation stages based on the presence of cell surface immunoglobulin also did not indicate apparent difference in expressed V_(H) gene family profiles. B cells in fetal spleen as an another hematopoietic lymphoid tissue in fetus also expressed similar V_(H) gene repertoire to that in fetal liver B cells. This distinct pattern of V_(H) gene expression in fetal B cells from that of adult B cells were not changed even after four weeks contact with adult bone marrow microenvironment supplied by the established adult bone marrow stromal cell layers. Thus, the restricted V_(H) gene repertoire of B cells in fetus which is distinct from that in adult appears to be associated more with the genetic potential of fetal B cell progenitors and less with environmental influences or differentiation stages or compartmentalization.
정준기(Joon Ki Chung),정현도(Hyun Do Jeong),하재이(Jai Yi Ha),허민도(Min Do Huh) 한국어병학회 1992 한국어병학회지 Vol.5 No.2
The absorption and excretion times of oxolinic acid(OX) used in farms as new aquatic antibiotics commonly were evaluated with determination of the effects of water temperature and feeding to parameters by using the bioassay technique. On the same time, antibacterial activity and the complex formation of oxolinic acid with serum proteins of two different fishes were compared to those oxytetracycline(OTC). With more than 10 times lower MIC values than those of OTC in the strains among 13 analyzed fish pathogens. OX did not show the decresed antibacterial activity by the binding of serum proteins in carp and tilapia. It implies more powerful potential of OX as aquatic medicine OTC. The serum concentration of OX after different administrations the oral, i.m., i.v and dipping methods were compared. The higher beginning concentration in serum and faster excretion times were obserbed in i.m. and dipping methods respectively. In the oral and i.m. administration, peak serum concentration after 24-48 hrs and slow excretion times demonstrated in both methods. These pharmacokinetic characteristics similar at 30℃ and 20℃ water temperature conditions, however, beginning serum concentration of OX in fish dipped in 50㎎/ℓsol after starvation for 2 wks was appeared lower than those of fed fish. It suggests the importance of biological condition of the gill or skin for absorption of antibiotics after dipping administration.
수온과 세균 감염이 나일 틸라피아 ( Oreochromis niloticus ) 의 oxolinic acid 흡수와 배설에 미치는 영향
김명석(Myoung Sug Kim),박성우(Sung Woo Park),허민도(Min Do Huh),정현도(Hyun Do Jeong) 한국수산과학회 1998 한국수산과학회지 Vol.31 No.5
The distribution and elimination of oxolinic acid(OA) following oral (80 ㎎/㎏ body weight) and bath(20 ㎎/ℓ) treatment were examined in the organs and plasma of Nile tilapia (Oreochromis niloticus) on different temperature and physiological conditions using a bioassay method. On the analysis of temperature effect, both absorption and elimination of OA after oral administration were delayed in the group at 15℃, but significant difference of the peak concentrations in the tissues of the groups at 15℃ and 25℃ was not revealed. However, the changes of maximam concentration absorption and elimination rates in the tissues of fish following both treatment depending on different temperature were more significantly different from the results of the studies with oral administration. The pharmacokinetics of OA in the tissues of diseased fish, the main target of drug treatment, also appeared to be distinguishable from those of healthy fish.
하재이(Jai Yi HA),손상규(Sang Gyu SOHN),허민도(Min Do HUH),정현도(Hyun Do JEONG) 한국수산과학회 1996 한국수산과학회지 Vol.29 No.5
To avoid the self-agglutination of Staphylococcus aureus sensitized with rabbit antibody in the absence of antigen, we determined the optimum concentration of rabbit antibody for sensitization. It was analyzed by using three different kinds of S. aureus strains at various concentraions of antibody. The optimized coagglutination test using the S. aureus sensitized with rabbit antibody was applied to the diagnosis of edwardsiellosis in field and in laboratory. The presence of E. tarda as low as 10 ㎍/㎖ was detected by this method. Moreover, it showed good coagglutination results against several different forms of antigens such as FKC, EDTA or heat extracted antigen of E. tarda. E. tarda strains, isolated from the flounders suffering from edwardsiellosis in fields, showed some cross-reactions to the E. tarda 219 analyzed by both agglutination and coagglutination test with rabbit anti-E. farda 219 antibody. The degree of cross-reactions analyzed was enough to apply the coagglutination test for the diagnosis of edwardsiellosis in field. Thus, even 1,000 fold diluted tissue homogenate of infected flounder naturally contained enough E. tarda as an antigen to show good coagglutination with S. aueus sensitized with rabbit anti-E. tarda 219 antibody. The successful application of this method to the homogenate and heat extract of tissues from naturally or artificially infected flounder or tilapia proved that coagglutination test was a simple and rapid reliable dignostic technique suitable for using in laboratory and field without any special equipments.
Edwardsiellosis의 진단을 위한 정성적 ELISA법
정현도,허민도,김명석,황은아 한국어병학회 1999 한국어병학회지 Vol.12 No.1
어류의 edwardsiellosis를 진단하기 위한 solid phase ELISA법에 대한 최적화 분석을 실시하였다. 부분 정제한 E. tarda Edk-2에 대한 토끼 항혈청을 sodium bicarbonate 완충용액에 50㎍/㎖ 농도로 희석하여 overnight 반응시켜 주었을 때 polystyrene bead 표면의 항체 immobilization이 최적화되었다. 50㎍/㎖의 biotin 표지화 항체와 1:2000으로 희석된 extravidin-peroxidase를 차례로 처리하였을 때 최적의 반응을 나타내었으며 이렇게 최적화시킨 정성적 solid phase ELISA법은 EDTA 추출법으로 조제된 항원에 대해서는 1×10^5 cells/㎖, 열 추출법으로 조제된 항원에 대해서는 5×10^5 cells/㎖의 검출한계를 나타내었다. E. tarda Edk-2에 대한 토끼 항혈청을 이용한 본 연구의 solid phase ELISA법은 우리나라 양어장의 넙치 병어로부터 분리한 여러 한국형 E. tarda 균주와도 높은 교차반응을 나타내었다. 이러한 것은 본 기법을 다른 지역에서 분리한 여러 strain의 E. tarda에 대한 진단을 위하여 본 실험의 항혈청을 사용하여 가능하다는 것을 보여 주었다. Optimization and standardization of solid phase enzyme immunoassay were done for the diagnosis of edwardsiellosis in fish. The analyzed degree of immobilized antibody on surface of solid phase with peroxidase saturation method showed the optimized result by using partially purified 50 ㎍/㎖ of rabbit anti-E. tarda Edk-2 antibody in sodium bicarbonate buffer for overnight incubation to cover the surface of polystyrene beads. Optimized immunoreaction was observed in the treatment of 50 ㎍/㎖ of biotin conjugated antibody followed extravidin-peroxidase diluted 1 : 2,000 in PBS. The detectable concentrations of the this method were 1 × 10^5 cells/㎖ and 5 × 10^5 cells/㎖ expressed as the source of antigen amount for EDTA extraction and heat extraction, respectively. High cross-reaction of solid phase ELISA with the prepared rabbit and-E. tarda Edk-2 was observed against E. tarda strains isolated from flounder suffering from edwardsiellosis in aquatic farms of Korea. It suggested that the potential of this solid phase of ELISA technique is very powerful for the application to different strains of E. tarda isolated in farms of many different areas.
정현도,허민도 한국어병학회 1993 한국어병학회지 Vol.6 No.1
아가미는 체조직 중에서도 가장 섬세하고도 미묘하게 적응된 조직학적 구조를 갖고 있을 뿐 아니라 환경수에 직접 노출되어 있기 때문에 수중의 각종 생물학적 및 물리화학적 유해인자에 쉽게 영향을 받을 수 있는 곳이다. 아가미를 구성하는 조직성분중에서도 특히 이차새변상피는 단층 내지 두층의 얇은 상피세포로 구성되어 있어 효율적인 가스교환을 할 수 있게 하지만, 이러한 구조의 특수성이 오히려 각종 유해 인자의 침입 또는 이들에 의한 손상을 쉽게 할 수 있다. 아가미는 호흡기능 뿐만 아니라, 장 및 신장과 함께 삼투압의 조절, 노폐물의 배설의 기능도 갖는다. 그러므로, 새변상피의 괴사나 비후와 같은 형태학적 변화는 호흡, 분비 및 배설기능의 장애를 야기할 수 있으므로 새변상피의 기능적 및 형태학적 유지는 무엇보다 중요하다 하겠다. 여기에서는 어떤 특정질병 또는 원인체에 관련한 병리조직학적 변화보다는 일반적으로 아가미조직의 조직학적 특수성을 우선 깊이 이해하고, 그 특성에 관련된 비특이적인 병리조직학적 변화를 중심으로 기술함으로써, 특정 원인체와의 병리조직학적 반응에 대한 보다 정확한 해석을 기하고자 이미 보고 및 출판된 정보를 토대로 하여 여기에 정리하였다. The delicate histological structure of gill in teleosts can be easily affected by a variety of biological, chemical or physical detrimental agents because it is directly exposed to the surrounding water. The epithelium of secondary lamella is thin to allow efficient gaseous exchange and this also renders it particularly vulnerable to various pathogens. As well as the main respiratory role, the gill has other various important functions such as acid-base balance. osmoregulation or the excretion of nitrogenous waste products. Thus destruction of epithelial integrity such as epithelial necrosis or thickening can render a fish very vulnerable to respiratory, secretory and excretory difficulties. This article was tried to describe in detail the common processes of pathological responses correlated to the normal histological structures of the gill in teleosts.