Whit Spot BaculoVirus (WSBV) 미감염 새우종묘 생산

허문수,손상규,김종식,손홍주 한국생명과학회 2001 한국생명과학회 학술발표회 Vol.31 No.-

부동산 셀링포인트에 관한 일고

허문수,이창석 강남대학교 사회과학연구소 2009 사회과학논총 Vol.25 No.-

Vibrio vulnificus ATCC 27562의 16S rRNA 유전자의 PCR과 제한효소절단 방식

허문수,정초록 한국생명과학회 1998 생명과학회지 Vol.8 No.2

A pair of designed primers (sequences from Gene Bank) amplified 16S fRNA gene of V. vulnificus within polymerase chain reaction (PCR) machine. This PCR product is about 1.3kb DNA fragment. Six enzymes (BamH I, Alu I, Sau3A I, Hind III, Sal I, Sma I) were used for restriction pattern analysis of amplified 16S rRNA gene of V. vulnificus ATCC 27562. Digested fragments are resolved by 3% agarose gel. BamH I did not show digested fragment so, there was no cutting site of BamH I in PCR product. Alu I produced three small fragments from 400 bp to 200 bp. Sau3A I produced three fragments larger than Alu I from 70 bp and 500 bp. One of fragments of Sal I was same with 500 bp of Hind III fragment and the other was 750 bp. Sma I showed two fragments of 800 bp and 470 bp. The profile of digested fragments of 16S rRNA of V.vulnificus ATCC 27562 will may be able to use standard profile for identification of V. vulnificus. Vibrio unlnificus ATCC 27562의 16S rRNA 유전자를 PCR법과 제한효소절단법으로 분석 하여 얻은 결과는 다음과 같다. 1. 고안된 한쌍의 primer로 PCR을 시행하여 얻은 산물은 약 1.3Kb 였다. 2. PCR산물을 여섯가지의 제한 효소로 절단하여 얻은 단편들은 아래와 같다. BamH I: 어떤 restriction fragment도 만들지 않았다. Alu I : 약 400bp와 200bp의 두가지 fragment를 생산하였다. Sau3A I : 약 70bp에서 450bp 사이에 세가지 fragment를 생산하였다. Hind III : 약 800bp와 500bp의 약간 큰 두가지 fragment를 생산하였다. Sal I : 약 500bp와 750bp의 두가지 fragment를 생산하였다. Sma I : 약 800bp와 470bp의 두가지 fragment를 생산하였다.

부동산경매제도의 절차상 문제점과 개선방안

허문수,이동영 한국부동산경영학회 2012 부동산경영 Vol.6 No.-

Real estate auction, a way for purchasing real estate, has a strong point- people can purchase real estate it for cheap price, but it has a weak point-people suffers from damage due to difficult right analysis. Recently, end-users through real estate auction have a high concernment. It is why they think auction to be a way of financial technology. But high concernment does not always bring about high success rate. As usual, in markets, there are more people suffering from failure than those who succeed in investment through real estate auction. People’s concernment on real estate auction increases gradually, but real estate auction is not activated, yet. As a result of material analyses, this thesis leaded to the result as followings. First, administrative officials have to be given the right of taking in and out, the right of investigation on occupants and debtors, and extensive compelling power to enforce to submit documents and to confirm subjectively inner structure and use. It will enable them to make written present condition. Second, if an auction procedure, aimed at carrying out legal rights of creditors, should be delayed, trust on national enforcement system is lowered. So, a disturbance of disposal by a complaint has to be got rid of with improved means. Third, the way to solve unpaid disposal is not standardized between courts. So, the cases have to be solved, basing on supreme regulations, for example the materials have to be disposed by special sale auction, that is, by delay interest rate added. In the end, as for the status of debtors, a lot of problems on laws relating to the current lease and the procedure of auction are exposed. The debtors’ seniority has to be secured. So, a item has to be established, saying that even if a debtor should win lessor in deposit return demand lawsuit and applies for auction for a real estate, counter power for the thirty party has not to be conceited, if only the lessor should dwell in the house till the time of auction application and deliver the house at the time of auction. As a result, the real status of the current real estate auction system operation and problems in carrying out it have to be continuously grasped continuously so that the system can be fixed as a system and improvement ways have to be established. Then people can trust the real estate auction system. In the future, the system improvement way has to be explored continuously.

부동산 경매제도의 문제점과 그 과제

허문수 강남대학교 사회과학연구소 2008 사회과학논총 Vol.24 No.-

학술발표회 : 8) 어병분야 , 구두발표 ; 양식대하 ( Penaeus chinensis ) 감염 White spot baculovirus ( WSBV ) 의 병원성 및 병리조직학적 특성

허문수,양병규,손상규 한국국제경제학회 2001 춘계 수산관련학회 공동학술대회발표 요지집 Vol.0 No.-

부동산 경매업의 현황과 실태분석

허문수,이창석 강남대학교 사회과학연구소 2009 사회과학논총 Vol.25 No.-

한국의 양식대하에서의 흰반점증상 바이러스감염의 특징

허문수,Heo Moon-Soo 한국생명과학회 2005 생명과학회지 Vol.15 No.2

대하 새우양식장의 대하에 흰반점 증상을 나타내는 원인바이러스는 막대형의 이중막을 가지고 있었으며, 전자 현미경 관찰 결과 평균 크기가 $250\~300\times70\;nm$였고, 조직학적 병변은 위상피 등에서 핵이 비대해지는 것이 관찰되었다. 공격실험에서는 건강체의 새우에 많은 누적 폐사율을 보였다. 원인 바이러스 단백질은 21개의 밴드를 보였으며, 핵산 분석 결과 total 분자량은 114 kb로 나타났다. The virions of causative virus for white spot syndrome in cultured Fresh shrimp, Penaeus chinensis were rod-shaped, double envelope. An average size of the virion was 70 nm in diameter and $250\~300$ nm in length. Histopathological test of affected stomach, heart, and lymphoid organ revealed nuclear hypertrophy. Infectivity trials carried out by injection and feeding with purified virus revealed high cumulative mortality to healthy shrimp. The twenty one different protein species were detected in the analysis of virion. The length of total DNA from the purified virus particles were detected as a single band, double-stranded DNA molecule of approximately 114 kb.

열 및 에탄을 shock 이 Virio vulnificus 의 막단백질에 미치는 영향

허문수,정초록 한국어병학회 1999 한국어병학회지 Vol.12 No.2

V. vulnificus ATCC 27562균주의 배양 온도를 47℃, 20분간 및 6% 에탄올. 10분간으로 반응시켰을 때 SDS-PAGE상에서 새로운 16가지의 heat shock protein(hsps)과 10가지의 ethanol shock protein이 나타났다. Lethal temperature에 노출하기전에 미리 열 충격을 가한 경우 thermo tolerance가 유도되었다. 균체면역에 의해 생성된 항혈청과 열 충격 세포에서 분리된 막단백질과의 ELISA에서는 Outer Membrane Protein(OMP)에서 높은 면역반응을 나타냈으며 western blotting으로는 Inner Membrane Protein(IMP)에서는 62kDa, OMP에서는 69 kDa단백이 높은 면역원성을 나타냈다. ethanol 충격 반응에서는 IMP에서는 48 kDa, OMP에서는 오직 major밴드에서만 면역반응성이 확인되었다. anti-V. vulnificus혈청에 대한 균체 응집시험에서는 열 충격 반응 후의 균체가 정상 균체에 비해 응집반응성이 높았다. New sixteen heat shock proteins (Hsps) and ten ethanol shock proteins were appeared on the analysis with SDS-PAGE when cultivation temperature for the Vibrio vulnifrcus ATCC 27562 strain was shifted-up to 42℃ from 30℃ for 20 mins and treated with of 6% ethanol for 10 mins, respectively. Even the induction of thermotolerance in V. vulnificus was coincided with the induction of Hsps if the pre-shock was adjusted to thermal temperature. Outer membrane proteins (OMPs) that were purified from the membrane of cells after heat shock showed more immunodominant pattern to the immunized rabbit anti-V. vulnificus O serum in enzyme-linked immunosorbent assay (ELISA). On the western immunoblot analysis it was confirmed that both 62 kDa IMP and 69 kDa OMP in the Hsps and 48 kDa IMP a major OMP in the ethanol shock proteins were reacted with rabbit anti-V. vulnificus O sera. Agglutination titer of the heat shocked V. vulnificus with rabbit anti-V. vulnificus O serum was higher than that of the untreated bacteria.

보리새우류의 바이러스 감염증의 진단

허문수,정초록,장경립 한국생명과학회 1999 생명과학회지 Vol.9 No.4

Baculovirus(WSBV) was isolated from infected Penaeide was collected from shrimp farm at southern sea of Korea from 1993 to 1995. The Infectious virus was purified and used for diagnosis of infected shrimp. Anti-viral serum were used for immunological detection as enzyme linked immunoabsorbent assay(ELISA) and indirect fluorescent antibody technique(IFAT). In IFAT, stomach, lymphoid organ and antenae gland of infected shrimp showed fluorescent reaction. In ELISA, tissues of spontaneously infected shrimp appeared higher O.D. values than in artificial infected shrimp. Primer set was constructed from sequence of 420bp of cloned Baculovirus(WSBV) genome. Specific band for infected shrimp was detected in Polymerase chain reaction(PCR)