당목향 뿌리 추출물의 인체 모유두세포 증식 및 모발 성장 관련 신호전달에 미치는 영향

최형철 ( Hyoung-chul Choi ),정노희 ( Noh-hee Jeong ) 한국공업화학회 2021 공업화학 Vol.32 No.6

In this study, Saussurea Lappa roots were extracted using ethanol and n-hexane, and also the effects on proliferation of human hair dermal papilla cells and fibroblast and related signaling pathway were evaluated. 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide (MTT) assay was conducted for cell proliferation effect of Saussurea Lappa root extract, and extracellular signal-related kinase (ERK), serine/threonine protein kinase (Akt), wingless-related integration site (Wnt)/β -catenin signaling pathway, and 5α-reductase expression through western blot analysis were measured. Saussurea Lappa root extract significantly increased human hair dermal papilla cells and propagation of fibroblast, promoted phosphorylation of ERK and Akt that get involved in cell proliferation. Additionally, Saussurea Lappa root extract significantly decreased promotion of Akt phosphorylation and cell proliferation by MEK/ERK inhibitor PD98059 and PI3K/Akt inhibitor LY294002. Also, Saussurea Lappa root extract induced intranuclear β-catenin accumulation by promoting phosphorylation of β-catenin (Ser552, 675) through phosphorylation of GSK-3β (Ser9), and suppressed activation of 5α-reductase type Ⅰ and Ⅱ. Overall, Saussurea Lappa root induces cell proliferation through vitalization of ERK and Akt route of human hair dermal papilla cells and fibroblast and apoptosis defense mechanism, and can be helpful in hair loss prevention and hair growth by vitalizing the β-catenin signaling pathway and inhibiting activation of 5α-reductase, which can be used as a potential hair care products.

개의 적출방광 평활근에서 Imipramine과 $K^+$ 통로 봉쇄제와의 상호작용

허준영,최은미,최형철,하정희,이광윤,김원준,Huh, Joun-Young,Choi, Eun-Mee,Choi, Hyoung-Chul,Ha, Jeoung-Hee,Lee, Kwang-Youn,Kim, Won-Joon 대한약리학회 1995 대한약리학잡지 Vol.31 No.2

The study was undertaken to examine the possibility of the involvement of $K^+$ channels in the mechanism of relaxant-action of imipramine on the isolated canine detrusor muscle strips. Canine urinary bladder were isolated, and smooth muscle strips of 15 mm long and 2 mm wide from the mid-portion of anterior wall were made in the Tyrode solution of $0{\sim}4^{\circ}C$. The strips were prepared for isometric myography in Biancani's isolated muscle chamber containing 1 ml of Tyrode solution, which was maintained with pH 7.4 by aeration with $95%\;O_2/5%CO_2\;at\;37^{\circ}C$. RP 52891, a non-specific $K^+$ channel opener, concentration-dependently suppressed the spontaneous phasic contractions of the detrusor strips. Imipramine, a tricyclic antidepressant, also reduced the spontaneous contractions in a concentration-dependent manner. RP 52891 was more potent than imipramine(p<0.05), and Imipramine was more efficient than RP 52891(p<0.05).Procaine, a voltage-dependent $K^+$ channel blocker, glibenclamide, an ATP-dependent $K^+$ channel blocker, and apamin, a calcium-dependent $K^+$ channel blocker antagonized the relaxant effect of RP 52891, but not of imipramine. Imipramine reduced the electric field stimulation (EFS) -induced contractions concentration-dependently. None of the $K^+$ channel blockers employed for this study, procaine, glibenclamide or apamin antagonized the inhibitory action of imipramine on the EFS-induced contraction. These results suggest that in canine detrusor, the $K^+$ channels of the characteristics of voltage-dependent, ATP-dependent and/or calcium-dependent are exist, and the inhibitory action of imipramine on the contractility of the detrusor is independent from the $K^+$ channels.

올레산 폴리프로필렌글리콜 에스테르류의 소포특성

이해연 ( Hai Yan Li ),최형철 ( Hyoung Chul Choi ),정노희 ( Noh Hee Jeong ) 한국유화학회 2011 한국응용과학기술학회지 Vol.28 No.2

In this study, by using oleic acid and polypropylene glycol, good natured antifoaming agent for suitable electronics process under the alkaline conditions were synthesized. For the synthesized mono and diesters, acid value, hydroxyl value was measured, and identified by FT-IR and 1H-NMR spectroscopy. Surface properties such as surface tension, critical micelle concentration(cmc) for diluted aqueous solution was measured, and tested the antifoaming properties according to the difference of alkyl chain length, various concentration, temperature and pH. The surface tension of synthesized antifoaming agent, PPMO(Polypropylene glycol monooleate) was 24.3 dyne/cm, PPDO(Polypropylene glycol dioleate) was 23.7 dyne/cm. By increasing of the alkyl chain length, surface tension was decreased slightly, and showed good antifoaming properties at 0.06 wt% concentration and 50℃, pH 11. These synthesized compounds are expected to apply as a suitable antifoaming agents in the semiconductor and the PCB(Printed Circuit Board) manufacturing process.

Thioacetamide 유발 급성 간손상에 대한 Diphenyl dimethyl dicarboxylate 의 효과

이헌주(Heon Ju Lee),최준혁(Joon Hyouk Choi),최형철(Hyoung Chul Choi),하정희(Jeoung Hee Ha),서정일(Jeong Ill Suh) 대한내과학회 1998 대한내과학회지 Vol.54 No.6

N/A Background: Diphenyl dimethyl dicarboxylate (PMC), a synthetic analogue of the natural product Schizandrin C which is well-known traditional Chinese herb, is used as a hepatoprotective agent. Thioacetamide (TAA) is a potent hepatotoxic agent which is metabolized by flavin-containing monooxygenase (FMO) in the liver, and its oxide leads to cellular damage. Protective effect of PMC for tissue injury was assessed on the basis of serological and histological changes in rats with TAA treated acute hepatic injury. Also, the effect of PMC on inflammatory tissue injury was assessed. Methods: The experiments were carried out on Sprague-Dawley male rats weighing 200-250g. PMC was administered intraperitoneally into normal rats, for 3 days, 100 mg/kg, once a day and the change of serum transa-minase was assessed. Rats were randomly assigned to two groups TAA group and TAA/PMC group. The first group received TAA, intraperitoneally 550 mg/kg, at once, The second group was subdivided into TAA/PMC II group and TAA/PMC II group. For TAA/PMC I group, PMC was administered intraperitoneally for 3 days, 100 ng, after TAA injection (intraperitoneally 550 mg/kg, once a day). For TAA/PMC II group, PMC was administered intraperitoneally for 5 days, 200 mg/kg, after TAA injection (intraperitoneally 550 mg/kg, once a day). The changes of serum trasaminase and histology for TAA and TAA/PMC group were assessed. To assess the effect of PMC for muscle injury, the extensor longus muscle was scissored and sutured. PMC was administered intraperitoneally for 3 days, 100 mg/kg, once a day. The change of leukocyte count and histology were assessed. Results: PMC significantly lowered the elevated serum transaminase (AST, ALT) due to intraperitoneal injection of TAA (550 mg/kg). Because PMC did not decrease the basal level of serum transaminase of normal rats, it can be suggested that PMC did not change serum transaminase production or degradation rate. It has really revealed through histological improvement that there was therapeutic effect of PMC on TAA treated acute hepatic injury. PMC brought histological improvement of muscle fibronecrosis and inflammatory change. Also, PMC decreased the elevated level of leukocyte by inflammatory reaction. Conclusion: These results suggest that PMC histologically protects the hepatocyte from injury by TAA, but the mechanism by which PMC protects against hepatic injury requires further investigation.

전자처리 및 Laser간섭에 의한 구조물의 Strain 측정에 관한 연구

정운관(Woon-kwan Jung),김경석(Koung-suk Kim),최정상(Jung-sang Choi),양승필(Seung-pil Yang),최형철(Hyoung-chol Choi),정현철(Hyun-chul Jung),김정호(Joung-ho Kim) Korean Society for Precision Engineering 1995 한국정밀공학회지 Vol.12 No.10

에탄올이 유기인제 농약에 의한 Cholinesterase 불활성화에 미치는 영향

최형철,김종호,하정희,이광윤,김원준,우현재,허창욱,손수민,천은진 영남대학교 의과대학 1999 Yeungnam University Journal of Medicine Vol.16 No.2

Background: In korea the agricultural community widely uses organophosphorous, and organophosphorous poisonings are increasing every year. We compared change in activity of acetylcholinesterase and pseudocholinesterase by organophosphorous and by the interaction of ethanol and organophosphorous. We also compared the effect of reversible anticholinesterase drugs, physostigmine and neostigmine. The object of this study is to investigate the effects of several anticholinesterase drugs and on how ethanol influences the activity of cholinesterase. Materials and Methods: Fifteen male university students were randomly selected, and blood samples were taken from the antecubital vein. The acetylcholinesterase in the RBC and the pseudocholinesterase in the serum were extracted and separated. The enzyme activity change was measured by the electrometric method. After adding acetylcholine, the pH change was measured with a pH meter. Results and Conclusion: Our results indicated that reversible anticholinesterase drugs decreased the cholinesterase activity more efficiently than organophosphorous. The acetyl cholinesterase and pseudocholinosterase activity were decreased by ethanol. When ethanol was added, oxime a cholinesterase activator, increased acetylcholinesterase activity but does not increased pseudocholinesterase activity.

새로운 조성을 갖는 고형 탈묵제의 제조

최형철,남기대,정노희 한국공업화학회 2003 공업화학 Vol.14 No.3

펄프섬유로부터 잉크입자를 효율적으로 제거하여 잉크함량이 적고 백색도가 높은 재생펄프용 탈묵제를 제조하였다 본 연구의 또 다른 목적은 고백색도에 필요한 여러 계면활성제의 적정조건을 찾고 재생펄프의 수율을 높이는 것이다. 본 연구에서 우리는 우수한 탈묵효과를 위해 스테아르산과 여러 유형의 계면활성제를 사용하였다. 그리고 탈묵효율에 미치는 여러 가지 고형 탈묵제의 효과를 알기 위하여 부상탈묵실험을 수행하였다. 그 결과 고백색도와 높은 수율을 얻을 수 있는 새로운 고형 탈묵제의 적절한 조합조성은 스테아르산 75%와 폴리옥시알킬렌알킬아릴에테르 6%를 혼합한 것이었다. 또한 탈묵효과를 폭넓게 적용하기 위해 기포력과 피 안정도, 분산능을 테스트하였다. 새로운 타입의 탈묵제는 폐지 탈묵시 우수한 분산능과 적당한 기포력과 안정성을 나타내었다. A new feinting agent for recycling of waste paper was produced. This agent efficiently removed ink particles from the pulp fiber and the resulting recycled pulp had a high brightness and a low content of residual ink. Efforts were made to find an optimum condition of various surfactants used for high brightness and yield of recycled pulp. In this study, stearic acid was used for various types of general surfactants for deinking. Flotation deinking experiments were conducted in order to examine the effect of various solid-type deinking agents. As a restult, an optimum combination of this new solid-type deinking agent, which resulted in high brightness and yield, was 75 wt% stearic acid mixed with 6wt% polyoxyalkylene alkyl aryl ester. This new type deinking agent had good dispersability, suitable foaming power and foam stability in deinking of waste paper.

세균 내독소 유발 혈관 저반응성에 대한 NG-nitro-L-arginine methyl ester와 Methylene blue의 영향

손의동,이광윤,하정희,김원준,최형철 영남대학교 의과대학 1997 Yeungnam University Journal of Medicine Vol.14 No.2

세균 내독소에 의하여 발생하는 패혈성 쇼크와 혈관 반응성 감소의 원인을 관찰하였다. 혈관 절편이 고정된 실험조에 세균 내독소 0.2mg 투여한 경우 36±3.65 nM NO가 발생되었고, NO 발생에 의한 혈관 이완효과를 억제하기 위해 전처치한 L-NAME, methylene blue는 혈관 절편의 phenylephrine (PE) 유발 수축 반응을 증가시켰으며 methylene blue에 의해 더 강한 수축 반응의 증가가 관찰되었다. 이때 혈관 내피세포가 존재할 경우에 PE에 대한 혈관 반응성이 증가되는 경향을 나타내었다. 세균 내독소 투여에 의해 acetylcholine 유발 혈관 이완은 증가되는 경향을 나타내었고, 전처치한 L-NAME, methylene blue에 의해 혈관 절편의 acetylcholine (ACh) 유발 이완은 억제되었으며 methylene blue에 의해 현저히 억제되었다. 그러나 세균 내독소를 투여하지 않은 군의 ACh 유발 혈관 이완 반응은 methylene blue에 의해서만 억제되었다. 결론적으로 세균 내독소에 의한 혈관 반응성 감소와 혈관 이완 반응은 NO가 발생되어 guanylyl cyclass를 활성화하여 유발된다고 생각되며, 세균 내독소에 의한 효과는 L-arginine·NO pathway 보다는 cyclic GMP 신호전달계를 경유한 경로에서 더 많은 영향을 받는 것으로 사료된다. This study was undertaken to examine the intensity of involvement of inducible nitric oxide synthase (iNOS) and cyclic GMP signal transduction pathway as one of the mechanisms of vaso-relaxative action of bacterial lipopolysaccharide (LPS) on the canine femoral artery strips. Canine femoral arteries were isolated and spiral strips of 10 mm long and 2 mm wide were made in the Tyrode solution of 0-4℃. The strips were prepared for isometric myography in Biancani's isolated muscle chamber containing 1 ml of Tyrode solution, which was maintained with pH 7.4 by aeration with 95% O₂/5% CO₂ at 37℃ and nitric oxide (NO) production was measured simulltaneously with isolated nitric oxide meter. LPS induced NO production, suppressed the phenylephrine (PE) induced contraction and enhanced the acetylcholine (ACh) induced realxation. NG-intro-L-arginine methyl ester (L-NAME), an NOS inhibitor, methylene blue, a guanylyl cyclase inhibitor, potentiated PE induced contraction and suppressed ACh induced relaxation on the LPS treated strips. The inhibitory potency of methylene blue for LPS induced vascular hyporesponsiveness was stronger than that of L-NAME. These results suggest that in canine femoral artery, both iNOs and cyclic GMP signal trnasduction pathway are related with LPS induced vascular hyporeponsiveness, but in minor with iNOS and in major with cyclic GMP signal trnasduction pathway.

항콜린에스테라제 약물의 소화관 운동성에 대한 영향

최형철,김종호,하정희,이광윤,김원준,곽동석,김성희,송필현,여지현 영남대학교 의과대학 1999 Yeungnam University Journal of Medicine Vol.16 No.2

Background: Anticholinesterase drug inhibits acetylcholinesterase(AChE), induce accumulation of acetylcholine(ACh) near cholinergic receptors and cholinergic stimulation. This experiment was performed to study the effects of anticholinesterase drugs on gastric motility and the effect of ethanol on anticholinesterase drug-induced motility change. Materials and Methods: After excision of stomach, 2×10mm circular muscle strips were made, which were then fixed to the isolated muscle chamber. An isometric tension transducer was used to measure the contraction change of the gastric smooth muscle strips after drug addition. Results: Fenthion, an irreversible anticholinesterase drug, increased ACh induced contraction of gastric smooth muscle strips and PAM, a cholinesterase activator, antagonized this action. Physostigmine, a reversible anticholinesterase drug, also increased the ACh induced contraction. The gastric motility was decreased by PAM. Ethanol, which is known to induce smooth muscle relaxation, inhibited the increase of contraction by fenthion. Conclusion: These results indicate that irreversible and reversible anticholinesterase drugs increase gastric motility and antagonized by cholinesterase activating drugs. And when exposed to both ethanol and anticholinesterase drug, gastric motility was decreased by the smooth muscle relaxation effect by ethanol.

전립선 기질세포의 증식과 COX-2 발현에 대한 프로게스테론의 영향

정수련,김성한,최이화,박지은,전은미,강영진,이광윤,최형철 영남대학교 의과대학 2006 Yeungnam University Journal of Medicine Vol.23 No.1

전립선비대증은 노인 남성에서 흔히 유발되는 질환이며, 노화가 진행될 수록 빈도가 높아지는 특징을 가진다. 이 질환의 원인은 전립선기질세표의 과도한 증식으로 유발된다고 알려져 있지만 그 자세한 기전에 대해서는 잘 알려져 있지 않다. 전립선비대증에서 progesterone 수용체 양성 세포가 다른 전립선 종양에 비해서 많고, progesterone은 testosterone에서 DHT로 전환되는 것을 감소시키는 역할을 가진다고 알려졋다. 또한 남성 전립선 평활근의 과증식에 의한 질환이므로 평활근 세포의 증식과 관련성이 있다고 보고된 COX-2의 전립선비대증에 대한 영향에 대한 연구가 필요하다. 전립선 기질세포에 progesterone을 3일간 투여하여 배양한 경우 기질세포 증식은 차이가 없었다. Progesterone을 단독 또는 DHT와 같이 투여한 기질세포에서 남성호르몬 수용체 mRNA 발현은 비처리군과 비교하여 유의한 차이가 없었다. 또한 progesterone과 DHT 동시 투여에 의한 COX-2 mRNA 발현에도 차이가 없었다. 그러나 progesterone에 의한 남성 호르몬 수용체와 COX-2 단백 발현에서는 대조군과 비교하여 유의하게 감소시켰다. 이상의 결과는 progesterone은 남성호르몬 수용체에 대해 전사 후 반응 (post-transcriptional response)에 효과를 나타내어 남성호르몬 수용체 발현을 감소시키는 작용은 가지며, COX-2 발현 억제효과를 나타내므로 전립선비대증의 치료에 이용될 수 있을 것으로 사료된다. Background: Benign prostatic hyperplasia (BPH) is the most common benign tumor in older men; the etiology of this disease remains poorly understood. Testosterone and dihydrotestosterone (DHT) both act as androgen via a single androgen receptor. Testosterone is converted to DHT by 5α-reductase in prostatic stromal cells. Progesterone has been reported to inhibit DHT conversion; howevwe, its effect on prostatic stromal cells remains to be elucidated. Materials and Methods: In this experiment, we investigated the effect of progesterone on androgen receptor expression induced by DHT. We also tested the effect of progesterone on cyclooxygenase-2 (COX-2) expression, as well as prostate stromal cell proliferation using the cell count kit-8. Results: Progesterone did not cause an increase of prostate stromal cell proliferation. The mRNA expression of the androgen receptor and COX-2 were not changed by progesterone; the expressions of androgen receptor and COX-2 proteins were decreased by progesterone in prostate stromal cells. Conclusion: These results suggest that in prostate stromal cells, progesterone decreases androgen receptor protein expression, which results in decrement of COX-2 protein expression. This effect might be mediated by post-transcriptional regulation.