http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
소 체내포 핵이식에 의한 핵-세포질 상호작용에 관한 연구
정희태,최종엽,박춘근,김정익,민동미 韓國受精卵移植學會 2000 한국동물생명공학회지 Vol.15 No.1
This study was conducted to investigate the effects of quiescent treatment of donor cells and activation treatment time of recipient cytoplasm on nuclear remodeling and in vitro development of somatic cell-cloned bovine embryos. Serum starved, confluent and nonquiescent cycling adult skin cells were teansferred into enucleated oocytes. Nuclear transfer oocytes were activated at 30 min, 1 and 2 hrs after electrofusion. Some nuclear transfer embryos(23% to 35%) extruded a polar body, which was not affected by quiescent treatment of donor cells and activiation time of recipient cytoplasm. About 68% of nuclear transfer embryos fused with a serum starved cells has a chromatin clump, but which was not different from embryos fused with confluent(51%) and nonquiescent(47%) cells. The proportion of embryos with a single chromatin clump was sightly increased when nuclear transfer embryos were activated within 30 min after fusion(69%) compared to those were activated at 1 and 2 hrs after fusion, but there was not significantly different. Development rates to the blastocyst stage were 8.6% and 15.9% when serum starved and confluent cells were transferred, which were higher than that of control group. Developmental rate to the blastocyst stage was higher in embryos were activated within 30 min after fusion (17.3%) compared to those of embryos were activated at 1 and 2 hrs after fusion (P<0.05). From the present result, it is suggested that quiescent treatment of donor cells and activation time of recipient cytoplasm can affect the in vitro development. Quiescent plasm activation within 30 min after fusion could increase the number of embryos with a normal chromation structure, which results in increased in vitro development.
양윤희,최종엽,이상영,박춘근,양부근,김정익,정희태 한국동물생명공학회(구 한국동물번식학회) 2003 Reproductive & developmental biology Vol.27 No.3
본 연구는 난자의 성숙시간, PHA-P 처리 또는 활성화 방법이 소 미수정란의 탈핵, 재구축란의 융합, 활성화 또는 체외발육에 미치는 영향을 검토하였다. 미수정란은 성숙 후 16∼24시간에 탈핵을 실시하고, PHA-P 처리 또는 무처리된 귀 피부세포를 이식 후 전기융합을 실시하였다. 후자의 경우는 융합 전에 PHA-P로 15분간 배양하였다. 융합란은 A23187과 CHXM 혹은 DMAP의 병용처리에 의해 활성화를 유기하고, 7∼9일간 체외배양하였다. 탈핵율은 성숙 후 16∼18시간에 실시한 경우(70.2∼92.3%)가 성숙 후 20∼24시간(44.3∼3.4%)에 비하여 유의적으로 높았다(P<0.05). M-II기 염색체의 위치는 성숙배양 시간이 길어짐에 따라 제 1 극체와의 간격이 멀어졌다. Donor 세포 혹은 재구축란에 PHA-P를 처리한 경우는 무처리구에 비하여 융합율이 향상되었다(P<0.05). 핵이식배의 분할율 및 배반포 발달율은 A23187+DMAP 처리구에서 78.6%와 32.9%로, A23187+CHXM 처리구에 비하여 유의적으로 높았다(P<0.05). 본 실험 결과는 성숙후 18시간에 탈핵을 실시하는 것이 효과적이며, donor세포 또는 융합 전 재구축란의 PHA-P 처리가 융합율 향상시킬 수 있고, 또한, 융합란을 A23187과 DMAP으로 병용처리 함으로써 난자의 활성화 및 배반포 발육율을 향상시켜, 결과적으로 핵이식기술의 효율성을 증진시킬 수 있을 것으로 사료된다. This study was conducted to examine the effects of oocyte maturation period, phytohemagglutinin-P (PHA-P) treatment and activation agent on the enucleation, fusion, activation or in vitro development of bovine nuclear transfer embryos. Bovine oocytes were enucleated at 16∼24 h of in vitro maturation (IVM). Adult ear skin cells treated or non-treated with PHA-P were transferred into enucleated oocytes. Reconstituted oocytes treated or non-treated with PHA-P were fused by a pulse of 1.5 kV/cm for 30 μsec. Fused oocytes were activated with a combination of calcium ionophore (A23187) and cycloheximide (CHXM) or dimethylaminopurine (DMAP), and cultured in vitro for 7∼9 days. Enucleation rate was significantly increased when oocytes were matured for 16∼18 h (70.2∼92.3%, P<0.05) compared to that of oocytes were matured for 20∼24 h (44.3∼53.4%). The location of metaphase-II plate was far off from the 1st polar body as maturation time was increased. PHA-P treatment of donor cells or reconstituted oocytes significantly improved fusion rate (P<0.05). Cleavage and blastocyst formation rates were significantly increased after activation with a combination of A23187 and DMAP (78.6% and 32.9%, respectively) compared to those of embryos activated with a combination of A23l87 and CHXM (48.5 and 15.2%, respectively). From the present result, it is suggested that high enucleation efficiency can obtained by using oocytes matured for 18 h. It also shows that PHA-P treatment can improve the fusion rate, and activation with a combination of A23187 and DMAP can enhance the embryo development.
수중 교전 모의를 위한 HLA/RTI 기반 시뮬레이션의 모니터링 시스템 구축 연구
함원경,정용호,최종엽,박상철,Hwam, Won K.,Chung, Yongho,Choi, Jong-Yeob,Park, Sang C. 한국시뮬레이션학회 2013 한국시뮬레이션학회 논문지 Vol.22 No.2
본 연구에서는 수중 환경에서의 교전급 모의를 위한 분산 시뮬레이션 시스템에서 모니터링 시스템을 설계 및 구축한다. 국방 분야에서 모델링 및 시뮬레이션(M&S)을 통한 검증은 비용, 시간, 및 노력의 측면에서 효율적인 접근 방법으로 주목되어 왔으며, 무기체계의 도입의 각 단계에서 시뮬레이션 기반의 획득(Simulation-Based Acquistion : SBA)이 필수 프로세스로 지정되었다. 그러나 복잡한 전장 환경을 전반적으로 묘사하는 방대한 시뮬레이션 시스템의 개발은 많은 자원을 요구한다. 따라서 이미 개발된 한정적인 목적을 갖는 시뮬레이터들을 조합하여 새로운 시뮬레이션 시스템을 구축하는 분산 시뮬레이션 시스템을 통해 시스템의 개발에 투입되는 비용 및 시간의 절약이 가능하다. 분산 시뮬레이션 시스템 중 High-Level Architecture(HLA)는 미국 표준으로 지정되었고, 이것을 구현한 Run-Time Infrastructure(RTI)는 미들웨어로서 효율적인 분산 시뮬레이션 시스템의 구축을 도와준다. 본 연구의 핵심 목적은 HLA/RTI 기반의 수중 교전 모의 시뮬레이션 시스템에서 수행 목적에 적합한 모니터링 요소를 도출하고, 그 체계 및 표현 방안을 설계하여 시뮬레이션을 분석하는 시스템의 구현에 있다. This paper presents design and implementation of the monitoring system for the distributed simulation of underwater warfare. As importance of defense modeling and simulation(M&S) has raised, Simulation-Based Acquisition(SBA) was authorized for an obligatory process in the development process of weapon systems. Yet, it requires tremendous resources to develop a large-scale simulation system that describes complex and broad battlefields. Therefore, an approach of the distributed system was devised to develop a new simulation system combining legacy simulators that were developed for confined purpose and sole operations. High-Level Architecture(HLA) of distributed systems is a standardized protocol by IEEE for the distributed simulation system and Run-Time Infrastructure(RTI) is an implementation of HLA to structure efficient distributed systems. The main objective of this paper is to derive appropriate monitoring factors for underwater warfare simulation, design and implementation of the monitoring system to analyze the factors based on HLA/RTI.