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조직배양에 의한 반하〔Pinellia ternata(Thunb.) Briet〕의 대량번식에 관한 연구
崔定植,羅義植 韓國作物學會 1986 Korean journal of crop science Vol.31 No.1
반하〔Pinellia ternata(Thunb.) Briet〕의 조직을 기내배양하여 callus 유기, 기관분화 및 자구를 증식시키는데 적합한 배지와 배양부위를 선정코저 Mur-ushige & Skoog 배지에 2,4-D와 kinetin의 첨가량을 달리하여 검토하였고 또한 배양온도 및 배지의 pH 영향을 조사하였던 바 그 결과를 요약하면 다음과 같다. 1. Callus 유기는 배양 5~7일 후에 엽조직 및생장점조직의 절단면에서 관찰할 수 있었으며 2,4-D 2.0mg/ +kinetin 0.2mg/ 을 첨가한 구에서 callus의 형성 및 생장이 가장 양호하였다. 2. 자구의 형성은 엽조직 및 생장점조직을 2,4 -D 2.0mg/ +kinetin 0.2mg/ 또는 2.4-D 2.0g/ +kinetin 2.0mg/ 을 첨가한 배지에서 양호하였고 근 및 괴경조직에서는 자구가 전혀 ?성되지 않았다. 3. 엽의 부위별 자구형성능력은 Petiole이 자구형성수, 크기, 엽면적 및 생체중에 있어서 marginal meristem, minor vein area 및 intercostal area 보다 양호하였다. 4. 경의 부위별 자구형성능력은 상부위 조직일수록 자구의 형성수 크기, 엽면적 및 생체중이 양호하였으나 하부위일수록 불량하였다. 5. 배양온도는 25℃ 에서 callus 유기, 크기 및 생체중이 가장 양호하였다. 6. 배지의 pH는 4.5~7.5에서 모두 callus가 유기되었으나 그중 pH 6.0 배지에서 가장 양호하였다. 7. 재생되는 식물체 일부가 광엽화되는 경향이었으며 소수의 albino 식물체와 변이체도 관찰할 수 있었다. In order to find out the best media, explants and environmental conditions for induction of calluses and organogeneses of Pinellia ternata (Thunb.) Breit in vitro, various parts of adult have been cultured on Murashige & Skoog's medium containing various levels of 2,4-dichlorophenoxy acetic acid(2,4-D) and kinetin. The results obtained were as follows: Calluses were induced from the surface of apical meristem and leaf tissue. Formation and growth of calluses in petiole ex plants were best on the MS medium complemented with 2,4-D 2.0 mg/l and kinetin 0.2mg/l. But callus formation in stem ex plants of the nearest tuber was not induced at all kinds of media. Plantlets occured at all treatment except absence of growth regulator. Their numbers, size, leaf and fresh weight were promoted by 2,4-D 2.0mg/l and kinetin 0.2mg/l. Root growth was increased on the medium containing higher 2,4-D concentrations. Size and fresh weight of callus were increased at 25~circC compared with 10, 20 and 30~circC , respectively. Optimal pH value was at 6.0 for growth of callus. Morphological aberrations were observed in plantlets, especially in regenerated leaves. The separation of the broad leaved plantlets and albino were observed in some cultures. Growth of plantlets after transplantation was best in pots with the sterilized vermiculte. But abnormal variants withered up.
半夏의 組織培養에 關한 硏究 : II.器官分化의 組織學的 硏究 II.Histological Studies on the Organ Differentiation
崔定植,羅義植,金永斗,韓光洙 전북대학교 농업과학기술연구소 1986 農大論文集 Vol.17 No.-
This study was carried out to investigate the histological origin of callus, clone, shoot, and root of Pinellia ternata(Thunb.) Breit, known as a medicinal crop. The results obtained were as follows: 1. Callus was induced from the cutted section of leaf tissue. 2. Shoot formed from the subepidermis of leaf explant. 3. Adventitious root were formed from callus tissue or base of shoots. 4. Contractile root forming root primodium was normal structure. 5. Shoot primodium was formed fast better than root primodium from meristemoid.
HAI 제어기에 의한 IPMSM 드라이브의 최대토크 제어
최정식,남수명,정동화 국립7개대학공동논문집간행위원회 2005 공업기술연구 Vol.5 No.-
Interior permanent magnet synchronous motor(IPMSM) has become a popular choice in electric vehicle applications, due to their excellent power to weight ratio. The paper proposed maximum torque control of IPMSM drive using hybrid artificial intelligent(HAI) controller. The control method is applicable over the entire speed range and considered the limits of the inverter's current and voltage rated value. Also, this paper proposed speed control of IPMSM using adaptive learning mechanism fuzzy neural network(ALM_FNN) and estimation of speed using artificial neural network(ANN) controller. The proposed control algorithm is applied to IPMSM dreve system controlled ALM-FNN and ANN controller, the operating characteristics controlled by maximum torque control are examined in detail. Also, this paper proposed the analysis results to verify the effectiveness of the ALM-FNN and ANN controller.
실습선을 이용한 주 추진기관의 배기배출물의 실시간 계측
최정식,이상득,이경우,천강우,남연우,윤석훈,최재혁,Choi, Jung-Sik,Lee, Sang-Deuk,Lee, Kyoung-Woo,Chun, Kang-Woo,Nam, Youn-Woo,Yoon, Seok-Hun,Choi, Jae-Hyuk 해양환경안전학회 2013 海洋環境安全學會誌 Vol.19 No.5
본 연구에서는 한국해양대학교 실습선 한바다를 이용하여 선박의 입 출항 및 약 150 rpm의 정속 운항 할 때 주기관에서 배출되는 배기가스를 실시간 계측하였다. 실측 결과 질소산화물의 농도는 정속 운항시 800 ppm 에서 1,000 ppm 사이인데 반해, 입 출항시에는 210 ppm 에서 1,230 ppm 까지 큰 범위에서 값이 변화하였다. 일산화탄소의 농도 역시 정속운항 상태 보다 입 출항시 측정값의 변화가 크게 나타났다. 이러한 결과는 입 출항시 가능한 한 주기관의 부하변동이 급격히 발생되지 않도록 하는 선박 조종 스킬이 필요하다는 것을 의미한다. 또한, 배기가스 저감 기술의 적용에 있어 입 출항시와 정속 운항할 때 그 차이를 고려할 필요가 있다는 것을 나타낸다. In this study, we conducted real-time measurement at the ship arrivals and departures at the port and at a constant speed of 150 rpm for exhaust emissions from a main engine installed on the training ship, HANBADA, of Korea Maritime University. The result showed that the concentration of nitrogen oxide was measured in the range of 800 ppm to 1,000 ppm at constant speed mode. On the other hand, the concentration of nitrogen oxide during ship arrivals and departures was significantly fluctuated between 210 ppm and 1,230 ppm. And, the concentration of carbon oxide at the arrivals and departures was also larger than that of at constant speed mode. These results show that the ship maneuvering skills to prevent a sudden load change of main engine at the arrivals and departures of ship is needed. Additionally, it means that the difference of exhaust emissions generated between the constant speed mode and the arrival/departure has to be considered when invented many technologies are adopted into the reduction technologies of air pollutants from ships.