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채창훈,Jinhyung Park 한국축산학회 2023 한국축산학회지 Vol.65 No.1
Climate change has worsened droughts and floods, and created conditions more likely to lead to pathogen contamination of surface water and groundwater. Thus, there is a growing need to disinfect livestock water. Ultraviolet (UV) irradiation is widely accepted as an appropriate method for disinfecting livestock water, as it does not produce hazardous chemical compounds and kills pathogens. However, UV-based disinfection inevitably consumes electricity, so it is necessary to improve UV disinfection effectiveness. Aluminum-based reflective nanolens arrays that enhanced the effectiveness of a continuous-flow UV water disinfection system were developed using electrochemical and chemical processes, including electropolishing and two-step anodization. A continuous UV disinfection system was custom designed and the parts were produced using a three-dimensional printer. Electropolished aluminum was anodized at 40 and 80 V in 0.3 M oxalic acid, at 120 and 160 V in 1.0 M phosphoric acid, and at 200 and 240 V in 1.5 M citric acid. The average nanolens diameters (D) of the aluminum-based reflective nanolens arrays prepared using 40, 80, 120, 160, 200, and 240 V anodization were 95.44, 160.98, 226.64, 309.90, 296.32, and 339.68 nm, respectively. Simple UV reflection behind irradiated water disinfected Escherichia coli O157:H7 in water more than did the non-reflective control. UV reflection and focusing behind irradiated water using an aluminum-based reflective nanolens array disinfected E. coli O157:H7 more than did simple UV reflection. Such enhancement of the UV disinfection effectiveness was significantly effective when a nanolens array with D 226.64 nm, close to the wavelength of the irradiated UV (254 nm), was used.
Electrochemical impedimetric biosensors for food safety
채창훈,오세욱 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.7
Electrochemical impedimetric biosensors (EIBs)have a simple structure and can be used to rapidly andsensitively detect and measure hazards in food. EIBs detectand measure target molecules by transducing biochemicalreactions on their surface to electrical signal outputsresponding to a sinusoidal electrical signal input. Due totheir structural simplicity and analytical sensitivity, EIBsare regarded as the most potent method of food hazardmonitoring that can be implemented in the food supplychain. This paper discusses the theoretical background,structure, and construction of EIB and its applications infood safety.
전력산업에서 VR/AR 기술동향 및 공통 플랫폼 구성방안 연구
채창훈,최민희,유은근,임찬욱,박진우,정남준,Chae, Changhun,Choi, Minhee,Yoo, Eungeun,Lim, Chanuk,Park, Jinwoo,Jung, Namjoon 한국전력공사 2016 KEPCO Journal on electric power and energy Vol.2 No.3
최근 세계적인 ICT 기업들이 가상현실 (VR)과 증강현실 (AR) 기술의 생태계 확장을 위하여 많은 노력을 하고 있다. 기존의 엔터테인먼트 분야뿐만 아니라 교육, e-커머스 등 다양한 산업으로 발전시키고 있으며, 전력, 에너지 분야 또한 예외가 아니다. 전력산업에서도 VR/AR 기술 적용을 통한 어플리케이션의 증가가 예상되고 있어, 관련 기술 요소들을 데이터와 프로세스 관점에서 표준화하여 재사용성을 높이고, 기존 Legacy 시스템 연동과 보안 등을 고려한 플랫폼 구성을 선행함으로써 어플리케이션 개발에 효율과 편의성을 극대화할 수 있을 것이다. 본 논문에서는 VR, AR의 기술개발 동향 및 전력산업에서의 적용가능성과 플랫폼의 필요성, 구성요소들을 도출하여 기능적으로 완성도 높고 사용이 편리한 다양한 전력 VR/AR 플랫폼을 제공함으로써 포켓몬고 (Pokemon GO)와 같은 전력분야의 혁명적인 킬러 어플리케이션의 도출과 플랫폼 표준을 이끌어갈 것으로 기대한다. Recently, many ICT companies are trying to expand the ecosystem of VR (Virtual Reality) and AR (Augmented Reality). They are developing a variety of industries such as entertainment, education, e-commerce and so on. There is also contains the power, energy field. And application related with VR/AR will increase in power industry. Thus, Platform is necessary because there are problems such as re-use, interface with legacy system and security. We can expect the convenience and efficiency by developing VR/AR power platform. In this paper, we will discuss the trends of VR/AR technologies, possibilities of VR/AR in energy field, essential elements of VR/AR power platform. In the future, we expect to lead standards of VR/AR in energy and to develop a killer application such as Pokemon GO.
Emulsion PCR to Improve Sensitivity of PCR-based E. coli O157:H7 ATCC 35150 Detection
채창훈,오세욱 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.4
Conventional PCR (cPCR) with 38 thermal cycles (cPCR38cycles) failed to amplify 3 copies of Escherichia coli O157:H7 DNA at the presence of 3.28×106 copies of Salmonella Typhimurium DNA, but emulsion PCR (ePCR) with 20 thermal cycles and a subsequent conventional PCR with 38 thermal cycles (ePCR20cycles-sPCR38cycles) amplified them. Partitioning individual E. coli O157:H7 DNA with emulsion droplet improved the specificity of PCR and let 3 copies of E. coli O157:H7 DNA to be amplified specifically at the presence of 3.28×106 copies of S. Typhimurium DNA. Application of ePCR improved the sensitivity of PCR-based pathogen detection and enabled the detection of 3 copies of E. coli O157:H7 DNA.
채창훈,장해진,오세욱 한국응용생명화학회 2016 Applied Biological Chemistry (Appl Biol Chem) Vol.59 No.1
Shigella sonnei shares many physiological aspects with Escherichia coli; thus, so far no culture-based method has been developed to detect and quantify S. sonnei separately from E. coli. Therefore, little information is available for the growth characteristics of S. sonnei in food. This study aimed to address a systematic scheme to quantify S. sonnei in beef separately from E. coli using quantitative real-time polymerase chain reaction (qRTPCR) and subsequently predict its growth characteristics. The use of S. sonnei-specific primers in qRT-PCR allowed to obtain growth curves of S. sonnei in beef at different temperatures, and the fitting of curves into a modified Gompertz model let us analyze the growth characteristics such as the lag time, maximum growth rate, and maximum quantity of S. sonnei in beef at different temperatures. A systematic scheme for RT-PCR-based quantification and a predictive modeling described in this study may be a useful means to analyze S. sonnei growth in food.
Principle of Emulsion PCR and Its Applications in Biotechnology
채창훈 사단법인 한국동물생명공학회 2019 한국동물생명공학회지 Vol.34 No.4
Emulsion polymerase chain reaction (PCR) is performed on compartmentalized DNA, allowing a large number of PCR reactions to be carried out in parallel. Emulsion PCR has unique advantages in DNA amplification. It can be applied in many molecular biological assays, especially those requiring highly sensitive and specific DNA amplification. This review discusses the principle of emulsion PCR and its applications in biotechnology. Related technologies are also discussed.