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저온 스트레스에 발현이 유도되는 콩의 L-asparaginase 유전자의 분리
박성환,김기영,진량,이재헌,Park, Seong-Whan,Kim, Kee-Young,Chen, Liang,Lee, Jai-Heon 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.2
Suppression subtractive hybridization (SSH)를 통해 저온에 의해 발현이 유도되는 cDNA들을 분리하였고, 그 중 하나인 slti182는 asparaginase 유전자들과 높은 유사성을 보였다. Slti182의 전체 cDNA인 GmASP1은 1258 bp 길이에 326개 아미노산으로 구성된 긴 Open reading frame (ORF)를 포함하고 있었다. GmASP1 단백질은 애기장대의 putative asparaginase (AB012247)와 84%의 유사성을 보였으나, 애기장대의 다른 isoform(Z34884)와는 55%의 유사성을 나타내었다. GmASP1 유전자는 저온 처리 후 3시간째부터 발현이 유도되어 6시간째에 최대발현을 나타내었고, 이후 점차 감소하여 48시간에는 저온처리전 수준으로 되돌아왔다. 또한, 식물체를 저온에서 48시간 둔 후에 다시 상온으로 옮겼을 때, 정상수준으로 떨어진 GmASP1의 발현이 다시 증가하는 양상을 보였다. 이러한 결과로 볼 때, GmASP1이 저온 스트레스에 반응 초기의 단백질 합성 촉진에 중요한 역할을 할 것으로 사료된다. Suppression subtractive hybridization (SSH) was used to isolate wound-induced cDNAs from wounded soybean. One of low-temperature-inducible cDNA, slti182 showed high homology with genes encoding 1-asparaginase. The full length cDNA of slti182, deginated GmASP1, is 1258 bp long and contains an open reading frame consisted of 326 amino acids. CmASP1 protein showed the highest identity (84%) with putative asparaginase from A. thaliana (AB012247), but it showed only 55% identity with another isoform of A. tathaliana (Z34884). The expression of GmASP1 during low temperature stress started to increase 3 hours after treatment, reached the maximum at 6 hour, and then decreased to the initial level at 48 hours. The amount of GmASP1 transcripts increased again when low-temperature-treated plants were transferred to room temperature, The present study suggests that GmASP1 may function to accelerate the protein synthesis which is important in the early response to low temperature.
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태권도 대회의 브랜드 자산 및 브랜드 태도가 재 참가의도에 미치는 영향
정지영(Chung, Ji-Young),진량(CHEN LIANG) 한국체육과학회 2023 한국체육과학회지 Vol.32 No.3
This study seeks to identify the relationship between brand equity, brand attitude, and re-participation intention of branding taekwondo competitions. The purpose is to provide basic data for marketing measures to vitalize Taekwondo competitions and strengthen competitiveness. A total of 336 copies of data were collected through Google forms targeting athletes participating in domestic taekwondo competitions, and a total of 324 copies of data were used in the final result processing, excluding 12 copies of questionnaires that were not suitable for research and insincere responses. As analysis tools, exploratory factor analysis, reliability analysis, correlation analysis, multiple regression analysis, and simple regression analysis were conducted through SPSS 27.0, and hypotheses established between each variable were verified. As a result of the analysis, the following findings were found. First, it was found that brand equity of Taekwondo competitions had a significant effect on brand attitude. Second, it was found that the brand equity of Taekwondo competitions had a significant effect on re-participation intention. Third, it was found that brand attitude had a significant effect on re-participation intention.
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Study on Legal Governance Guarantee of Digital Economy Development in China
XU YUAN(徐媛),CHEN LIANG(陳亮) 동아대학교 법학연구소 2021 國際去來와 法 Vol.- No.32
Disruptive technological change has made human society leap over the era of agricultural and industrial economy and ushered in the era of digital economy. The development of digital economy has become a key force in the implementation of Chinas major national strategy, which is of great significance to the implementation of supply-side reform and innovation-driven development strategy. European and American countries are committed to improving the strategic policies and supporting legal systems for the development of digital economy. China should learn from the successful experience of Europe and the United States, speed up the construction of a modern economic system, enhance its core competitiveness, establish a legal protection system for the development of the digital economy, and create Chinas first-mover advantage in the digital economy. This research systematically sorts out the development status and trend of Chinas digital economy, points out the legal dilemma of the development of Chinas digital economy from the perspective of the actual analysis and the ought to be analyzed, and makes a detailed analysis of the European Union and the United States key laws, regulations and policy planning for the development of digital economy. Finally, this research puts forward the basic conception of legal guarantee for the development of Chinas digital economy from three aspects of concept, principle and system.
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