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핵이식을 통한 양서류 역위배세포의 발생수행능력에 관한 연구
정해문,이자경,김덕희 한국유전학회 1987 Genes & Genomics Vol.9 No.1
In order to investigate the mechanism of early amphibian embryogenesis, the uncleaved eggs of Korean anuran Rana dybowskii were inverted at various times as well various duration after fertilization. However, developmental arrest at early gastrula stage always followed partial invagination of the dorsal lip. Nuclear transplantation was then employed to reveal the cause of death. Comparing the developmental capacity of the endodermal cells of inverted R. dybowskii and those of non-inverted normal control embryos, there was no difference between those two cases. It was concluded that the death of the inverted embryos was not attributed to the endodermal cells and the cause should be found elsewhere. Nuclear transplantations were also carried out to investigate the extent of development by newly injected nuclei from normal embryos. For that purpose nuclei from the cells at various developmental stages of blastula, gastrula, neurula and tail bud stages were injected into the eggs whose nuclei had been previously eliminated. It was revealed that as cells differentiated their nuclei progressively restricted in their ability to promote the development of enucleated eggs. The results are quite similar to those early observations by other investigators who used other amphibian species.
$3CaO.SiO_2$ 생성 반응과 미세조직에 미치는 $K_2SO_4$와 $Na_2SO_4$의 영향
정해문,한기성,최상흘 한국세라믹학회 1993 한국세라믹학회지 Vol.30 No.10
Effects of K2SO4 and Na2SO4 on C3S formation and its microstructure were investigated. C3S formation was not influenced by addition up to 6.0wt% of K2SO4 as SO3 base, however it was prevented by only 1.0wt% of Na2SO4 addition. C3S prevention by added Na2SO4 was the reason why C2S stabilized by Na+ and SO42- could not react to C3S. Added K2SO4 appeared as K2SO4, however added Na2SO4 appeared as the form of Na2xCa3-xAl2O6, (Na0.8Ca0.1)SO4 and Na2SO4 in interstitial phase.
분화와 발생양상의 조절기작에 관한 연구: 자외선 조사와 수정난의 회전이 배, 복축 극성의 결정에 미치는 영향
정해문 한국통합생물학회 1982 동물학회지 Vol.25 No.3
수정 직후 일어나는 배, 복측 결정의 기작을 알아 보기 위하여 자외선의 조사와 난의 회전방법을 한국산 무미양서류에 적용하였다. 제 1 분열전의 수정난을 일정기간 $90^\\cdot$회전시키면 자외선에 의한 전형적인 발생결함을 억제시킬수 있었다. 한편, 제 1 분열 전 단기간 회전시킨 난으로부터 발생한 embryo의 원구배순부의 위치도 회전시 중력의 반대 방향에 위치하였던 새로운 장소에 형성되었다. 위의 결과는 다른 무미, 유미양서류를 통하여 얻은 지식과 비교 검토하여 초기발생 기작을 이해하는데 접근하고자 하였다. A combination of ultraviolet irradiation and egg rotation was applied to Korean frogs to study the mechanisms of the establishment of the dorsal-ventral polarity. Rotation of the uncleaved egg was capable of preventing the characteristic syndromes associated with UV irradiation. As well, brief rotation of the egg before cleavage relocates the site of dorsal lip to a novel location in the embryo at gastrula stage. That is, the dorsal lip appeared on the opposite side to gravity at the rotation of the egg. Above results were discussed with the informations obtained from other anuran and urodele species, and were interpreted in terms of regulating mechanisms for early embryogenesis.