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말더듬 성인이 의도적인 느린 구어에서 나타나는 구어 속도의 변화
전희숙,권도하 대구대학교 특수교육재활과학연구소 2005 再活科學硏究 Vol.23 No.2
The purpose of this study lies in inquiring the change of speech rate in the concrete according to the method that stuttering adults who achieved fluency in a clinic intentionally make speech rate slower in this study. In this clinic room with developmental 10 stutterers who had well established fluency in colloquial speech, we had collected each 3-minute sample of intentionally much slowed speech and a little slowed one respectively from them in reading and monologue tasks as well, and then compared each speed rate after computing speech rate of first one-minute and last one-minute speech. The result of that study told us that the speed rate of each sample got faster. And in reading task was the speech rate of a little slowed speech faster than that of much slowed one, however, in monologue task was the difference of speech rate ignorable. And the seech rate in reading was faster than that in monologue. 본 연구의 목적은 임상실 내에서 유창성을 습득한 말더듬 성인이 의도적으로 구어 속도를 느리게 말할 때 구어 속도의 변화를 구체적으로 알아보고자 하는 것이다. 임상실에서 발달성 말더듬 성인 10명을 대상으로 조금 느린 속도와 많이 느린 속도로 읽기 및 독백 과제를 실시할 때 구어 샘플을 수집하여 처음 1분 동안과 마지막 1분 동안의 구어 속도를 비교하였다. 샘플에서 구어 속도의 변화를 비교한 결과 읽기 및 독백 모두 구어 속도가 빨라졌다. 많이 느리게 말한 경우와 조금 느리게 말한 경우 속도 변화를 비교한 결과, 읽기 과제에서는 속도 조절 방법에 따라 차이가 있었으나 독백에서는 차이가 없었다. 그리고 읽기보다 독백에서 구어 속도가 더 많이 처음 1분 동안의 속도에 비하여 더 빨라졌다.
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Cell Replacement and Regeneration Therapy for Diabetes
전희숙 대한당뇨병학회 2010 Diabetes and Metabolism Journal Vol.34 No.2
Reduction of beta cell function and a beta cell mass is observed in both type 1 and type 2 diabetes. Therefore, restoration of this deficiency might be a therapeutic option for treatment of diabetes. Islet transplantation has benefits, such as reduced incidence of hypoglycemia and achievement of insulin independence. However, the major drawback is an insufficient supply of islet donors. Transplantation of cells differentiated in vitro or in vivo regeneration of insulin-producing cells are possible approaches for beta cell/islet regenerative therapy. Embryonic and adult stem cells, pancreatic ductal progenitor cells, acinar cells, and other endocrine cells have been shown to differentiate into pancreatic beta cells. Formation of fully functional beta cells and the safety of these cells are critical issues for successful clinical application.
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대장균 내에서의 Bdi I Methylase 유전자의 클로닝과 발현
전희숙,김용석,최경래,노현모 한국미생물학회 1987 미생물학회지 Vol.25 No.1
B Brevibacterium divaricatum FERM 5948 균주로부터 Bdi I RIM 체계에 속하는 BdiI methylase 유천자를 클로닝하여 발현을 조사하였다. Bdi I methylase 유전자의 클로닝을 위해 pBR 322의 EcoRI, BamHl, Sal I 3 군데의 클로닝 site를 이 용했고 1 차 형질전환후 나온 플라스미드를 BdiI으로 자른 뒤 ligation 시키지 않고 형질전환시키는 방법을 이용하였다. 유전 자을 가지는 행질전환체의 선별은 Bdi I methylase에 의해 수정된 채조합 플라스미드는 BdiI 제한효소에 방호된다는 것에 기 초하여 선별하였는데 5.6kb의 EcoRI insert DNA를 가지는 pBDIM 116이 Bdil methylase 유전자플 가지는 것으로 판명 되었다. pBDIM 11&을 가지는 숙주셰포에서 추출한 추출용액에는 S-adenosylmethionine이 있으면 BdiI의 인지부위인 A ATCGAT에만 특정한 methylase 활성이 측정되였다. 11개의 제한효소를 이용하이 제한효소지도를 작성하였고, BdiI r restriction -modification 체계에 관해서 도 논의하였다. The gene for the Bdi I modification enzyme, which is one of Bdi I restriction-modification system, fromBrevibacterium divaricatum FERM 5948 was cloned and expressed in E. coli. For cloning of the Bdi I methylase gene, we have initially used three cloning site(EcoRI, BamHI and Sal I) of plasmid vector pBR 322 and adopted the retransformation method after Bdi I restriction endonuclease cleavage. Selection of transformants carrying the gene was based on the resistance of the modified plasmid encoding the enzyme to cleavage by Bdi I restriction enzyme, and the recombinant plasmid pBDIM 116 containing 5.6kb EcoRI insery was proved to carry the gene. Crude cell extracts prepared from strains carrying the plasmid pBDIM 116 contained an S-adenosylmethionine-dependent methyltransferase activity specific for the Bdi I recognition site, ATCGAT. The restriction map was constructed with 11 restriction enzyme, and the Bdi I restriction-modification system was also discussed.
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韓國 南西海岸地方의 抗菌性 放射線狀菌의 分布에 對한 調査
全喜淑,金成源 조선대학교 약학연구소 1982 藥學硏究誌 Vol.4 No.1
The antibiotic-producing streptomyces strains were isolated from the soils of various localities in Mokpo in the southwestern area of Korea and tested for the distribution and the activity against some microorganisms. The results are summarized as follows. 1. Total of 1471 streptomyces strains were isolated from 337 soil samples. 611 strains of the isolates showed antibiotic activity against the test organisms and 391 of the 611 strains were strongly active against those organisms. 2. 84 of streptomyces strains among the active isolates showed the antibiotic activity against Pseudomonas aeruginosa and 18 of 84 strains showed the antibiotic activity against all of the test organisms, i. e. Staphylococcus aureus, Bacillus subtilis, Escherichia coli. Shigella flexneri, Salmonella typhi. 3. Antibiotic-producing streptomyces strains active against Pseudomonas aeruginosa were isolated from the soil samples of vegetable fields, sewage disposal areas, roadways, and grounds but not isolated from the soil samples of dwelling areas, grass fields and piers. 4. 171 strains of the active isolates showed the antibiotic activity against gram-positive bacteria, but only 18 strains were active against gram-negative bacteria. 5. Strongly active streptomyces strains against the test organisms were isolated from the soil samples taken from roadways, vegetable fields, hills and grounds, but not isolated from the soil samples taken from piers.
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전희숙,고광삼 中央醫學社 1988 中央醫學 Vol.53 No.10
The isolation and, characterization of a new type II restriction endonuclease (Bdi I) from Brevibacterium divaricatum KCT 8035 were described. Bdi I. endonuclease was isolated by the following procedures; ammonium sulfate fractionation, DEAE-cellulose chromatography, phosphocellulose chromatography and hydroxylapatite chromatography. The isolated Bdi I restriction endonuclease was an isoschizomer of Cla I which recognizes 5 ATCGAT 3 , and cleaves between T and C within the recognition seguence. The optimal temperature on the enzyme activity was 37°C and optimal salt (NaCl) concentration was 50-100mM. Also Bdi I endonuclease reguired Mgr as a cofactor.
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