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Brassica 種들의 花뢰크기에 의한 小胞子 發育段階 및 胚發生 比較
장영석(Young Seok Jang),민경수(Kyung Soo Min),오용비(Yong Bee Oh),정동희(Dong Hee Chung) 한국육종학회 1997 한국육종학회지 Vol.29 No.4
The purpose of this study is to search for the identifing method that could separate buds with embryogenic potential from collected buds for increasing the efficiency of embryogenesis by microspore culture in Brassica species. It was investigated developmental stage of microspore by bud-size and ratio of petal/anther length and compared to amount of embryogenesis through isolated microspore culture from the buds with late-uninucleate stage. In Brasica napus, Lisandra of spring type showed bud-size of 3.2~4.1mm which belongs to late-uninucleate stage as embryogenic microspores and their range of petal/ anther length ratio were 1/2~3/4. Hallayuchae of winter type rapeseed showed bud-size of 3.1~3.6mm which belongs to late-uninucleate stage as embryogenic microspores and their range of petal/anther length ratio were 1/3~1/2. Two genotypes of B juncea had the same petal/anther length ratio as range of 1/2~3/4 with embryogenic microspores but their bud-size were some difference as bud-size of Young-sanpohwanggyeja were 2.9~4.0mm and bud-size of Hwanggyeja were 2.9~4.1mm. Also, two genotypes of B. campestris had the same petal/anther length ratio as range of 2/3~3/4 with embryogenic microspores but bud-sizes were some difference as bud-size of Hyakusai were 2.9~3.4mm and bud-size of Sosongchae were 2.9~3.2mm. In B. oleracea, the petal/anther length ratio with embryogenic microspores according to bud-size were showed range of 1/2~2/3 in all of two genotypes and bud-size of Broccoli were 2.4~3.2mm and bud-size of Cauliflower were 2.9~3.7mm. In the embryo yield per 100,000 microspores by isolated microspore culture from the buds with embryogenic microspores, Young-sanpohwanggyeja of B. juncea were 47 embryos and Hwanggyeja of B. juncea were 23 embryos, B. campestris of Hyakusai were 367 and Sosongchae were 123, B. oleracea of Broccoli were 678 and Cauliflower were 562. However in B. napus was showed much differences by the growth habit, the spring type of Lisandra were producted 7,047 embryos of which higher about 9.2 times than those of winter type Hallayuchae.
장영석(Young Seok Jang),민경수(Kyung Soo Min),오용비(Yong Bee Oh),정동희(Dong Hee Chung) 한국육종학회 1997 한국육종학회지 Vol.29 No.4
Microspore culture was carried out in order to compare the difference of embryo yield between spring type and winter type of Brassica napus L. and isolated microspores from three genotypes of winter type and three genotypes of spring type were cultured in modified Nitsch and Nitsch (NN) medium supplemented with 13% (w/v) sucrose, 0.05mg/ℓ BA and 1.0mg/ ℓ NAA. Temperature was 32.5℃ for 3 days of the initial stage in the dark and maintained at 25℃ in the dark. Embryo yields and frequency of normal embryo production showed remarkable contrast according to genotypes and growth habit. The highest frequency of embryogenesis was obtained from buds of 2.8~3.2mm in size approximately one week after first flowering and the buds of the sizes contained mainly microspores of late uninucleate stage. Embryos were induced from microspores isolated from the buds of 2.3~2.7mm, 2.8~3.2mm, 3.3~3.7mm and 3.8~4.2mm in the spring type. But only the buds of 2.3~2.7mm and 2.8~3.2mm were able to from embryos in the winter type.