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Nkx-2.5 Regulates MDR1 Expression via Its Upstream Promoter in Breast Cancer Cells
임정석,정규연,박승윤 대한의학회 2019 Journal of Korean medical science Vol.34 No.12
Background: Increased expression of MDR1 gene is one of the major mechanisms responsible for multidrug resistance in cancer cells. Two alternative promoters, upstream and downstream, are responsible for transcription of MDR1 gene in the human. However, the molecular mechanism regarding the transactivation of MDR1 upstream promoter (USP) has not been determined. Methods: Dual-luciferase reporter gene assays were used to assess the effect of Nkx-2.5 on MDR1 USP activity using reporter plasmids for human MDR1 USP and its mutants. MDR1 mRNA level was examined by quantitative real-time PCR. The direct binding of Nkx-2.5 to the USP of MDR1 was evaluated by promoter enzyme immunoassays and chromatin immunoprecipitation assays. Results: Nkx-2.5 significantly stimulates the transactivation of MDR1 USP and increases MDR1 mRNA expression in MCF7 breast cancer cells. Reporter gene assays with deleted MDR1 USPs showed that the Nkx-2.5-binding site is located between positions -71 and +12. Mutation of the Nkx-2.5-binding site at nucleotide +4 to +10 markedly reduced the Nkx-2.5-mediated activation of MDR1 USP activity. A promoter binding immunoassay and a chromatin immunoprecipitation assay revealed that Nkx-2.5 binds directly to the region +4/+10 of human MDR1 USP. Conclusion: The results in the present study show Nkx-2.5 is a positive regulator for the transactivation of MDR1 USP in MCF7 breast cancer cells. Our findings will help elucidate the regulatory mechanism responsible for the multidrug resistant cancer phenotype.
나카가미-n 페이딩 채널에서 직사각 QAM과 M-PSK 신호의 최대비 합성 수신 성능 비교
임정석,박상규,Lim, Jeong-Seok,Park, Sang-Kyu 한국통신학회 2005 韓國通信學會論文誌 Vol.30 No.8C
본 논문에서는 나카가미-n(라이시안) 페이딩 채널 상에서 그레이 부호화된 직사각 QAM(Rectangular-QAM) 신호를 최대비 합성(maximal ratio combining) 다이버시티로 수신하는 경우 비트노율(Bit Error Rate, BER)을 계산하는 수식을 다양한 채널에 적용할 수 있도록 일반적 형태로 유도하고 그 성능 분석을 실시한다. 유도된 BER 수식은 휘태커 함수)Whittaker function)와 초기하 함수(confluent hypergeometric)로 표현된다. 또한 M-PSK와의 성능 비교를 통하여 나카가미-n 페이딩 채널의 특성을 확인한다. BER 수식이 일반적인 형태로 유도되었기 때문에 가시 경로(line-of-sight)를 가지는 통신채널이나 위성통신 채널의 성능분석에 본 논문에서 유도한 BER 수식을 쉽게 적용할 수 있다. We derive and analyze a bit error rate(BER) expression of a Gray coded rectangular QAM(R-QAM) signal with maximal ratio combining diversity(MRC) reception over Nakagami-n(Rician) fading channels. The derived result is provided in terms of the Whittaker function and the confluent hypergeometric function. In addition, by performance comparison with M-PSK, we see the Nakagami-n fading channel characteristics. Because the derived expression is general, it can readily allow numerical e·valuation for various cases of practical interest such as line-of-sight (LOS) or satellite communication channel analysis.