http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Homocysteine 과다증과 관련된 반복 자연유산에서 Methylenetetrahydrofolate Reductase돌연변이에 대한 분석
남윤성,최종순,하권수,이지원,오도연,Nam, Yoon-Sung,Choi, Jong-Soon,Ha, Kwon-Soo,Lee, Zee-Won,Oh, Do-Yeon 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.3
Objective: To analyze the methylenetetrahydrofolate reductase (MTHFR) mutation in recurrent spontaneous abortion associated with hyperhomocysteinemia. Material and Method: The blood sample of habitual aborter with high fasting homocysteine level was tested by PCR - RFLP method. Results: The patient was found to be a homozygosity for MTHFR gene mutation that was confirmed by the finding which is consistent with the mutation at the nucleotide 677 C to T, corresponding to Ala to Val. Conclusions: Hyperhomocysteinemia due to MTHFR mutation is a cause of recurrent spontaneous abortion. Therefore, the MTHFR mutation should be examined in the workup of recurrent spontaneous abortion showing hyperhomocysteinemia.
Shin, Incheol,Lee, Zee-Won,Kweon, Soo-Mi,Leem, Sun-Hee,Kim, Jae-Hong,Kim, Byung-Chul,Ha, Kwon-Soo 東亞大學校附設遺傳工學硏究所 1999 遺傳工學硏究 Vol.- No.6
We have investigated possible roles of RhoA and H₂O₂ in the elevation of intracellular Ca²+[Ca²+]i) by phosphatidic acid (PA) in Rat-2 fibroblasts. PA induced a transient elevation of [Ca²+]i in nthe presence or absence of EGTA. Lysophosphatidic acid (LPA) also increased [Ca²+]i, but the sustained Ca²+ response was inhibited by EGTA. LPA stimulated the production of inositol phosphates, but PA did not. In the presence of EGTA, preincubation with LPA completely blocked the subsequent elevation of [Ca²+]i by PA, but not vice versa. PA stimulated the translocation of RhoA to the particulate fraction as did LPA. Scrape loading of C3 transferase inhibited the transient Ca²+ response to PA, but not to LPA, suggesting an essential role of RhoA in the elevation of [Ca²+]i by PA, H₂O₂ also induced a transient increase of [Ca²+]i as did PA. H₂O₂ scavengers, catalase and N-acetyl-L-cysteine, completely blocked the rise of [Ca²+]i stimulated by PA, but not by LPA. Furthermore, preincubation with PA blocked the subsequent Ca²+ response to H₂O₂, and the incubation with H₂O₂ also blocked the PA-induced rise of [Ca²+]i. Thus, it was suggested that PA stimulated Ca²+ release from PA-sensitive, but not inositol 1,4,5-trisphosphate-sensitive,Ca²+? stores by the activation of RhoA and intracellular H₂O₂.