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토복령알코홀추출물이 토끼 적출 십이지장관운동에 미치는 영향
이종화,김정일,송민자,Lee, Jong-Hwa,Kim, Chung-Il,Song, Min-Ja 대한약리학회 1973 대한약리학잡지 Vol.9 No.2
Chinae Rhizoma is dried crude roots and rhizomes obtained from Smilax china Linne which belongs to Smilax Genus of Liliaceae Familia, and it has been in wife use as a folk remedy for a long time. But very little systemic studies on its pharmacological actions have been made until now. Furthermore, the effective components except saponin of Chinae Rhizoma have been not known yet. It came to the attention of the authors that Chinae Rhizoma are used as antidiarrhetics in Chinese herb medicine, therefore they attempted to observe the effects of Chinae Rhizoma Alcoholic Extracts (Chinae Rhizoma Ethanol Extract=CREE, Chinae Rhizoma Methanol Extract=CRME) from Smilax china Linne on motility of the isolated rabbit duodenum in this experiment. The results of the study were as follow: 1. The movement of the isolated rabbit duodenum was slowed down and its tonus was relaxed in proportion to the concentration of Chinae Rhizoma Alcoholic Extracts (CREE & CRME). 2. With a concentration of CREE $10^{-4}g/ml$, the contractile effects of acetylcholine $10^{-6}g/ml$, pilocarpine $10^{-5}g/ml$ and physostigmine $10^{-5}g/ml$ were remarkably blocked in the movement of isolated rabbit duodenum. 3. The concentration of the isolated rabbit duodenum induced by barium chloride $10^{-4}g/ml$ was significantly inhibited by CREE $10^{-4}g/ml$. 4. With the administrations of histamine $10^{-5}g/ml$, serotonin $10^{-6}g/ml$ and prostaglandin $A_2\;10^{-6}g/ml$ respectively, contractions were observed in the rabbit duodenum. But, treatment of CREE $10^{-4}g/ml$ inhibited markedly the tonus and motility of the duodenum pretreated with the above drugs. 5. The effects of CREE and CRME were very much alike in this experiment. From the above results, it is concluded that the mechanism of action of Chinae Rhizoma on motility of isolated rabbit duodenum was partially due to cholinergic blocking effect.
흔들림 보정을 통한 고해상 사이드스캔소나의 데이터 관리기법 연구
이종화,김영석,박철,최상식,이흥수,Yi, Jong-Hwa,Kim, Young-Seok,Park, Chul,Choi, Sang-Sik,Lee, Heung-Su 한국구조물진단유지관리공학회 2017 한국구조물진단유지관리공학회 논문집 Vol.21 No.5
기존의 예인식으로 운용하는 사이드스캔소나의 경우 조사 시 사용자가 원하는 단면 조사에 어려움이 있어 수중구조물 전동 지그가 개발되었지만, 전동지그를 사용한 음파촬영방법의 경우 보트와 소나가 일체 거동하기 때문에 파랑에 의한 보트 롤링현상, 보트운전자의 운용미숙 등으로 인한 음파영상의 흔들림발생 등, 작업환경에 따른 여러 가지 문제로 인하여 데이터의 왜곡이 발생하였다. 따라서 본 연구에서는 기존 사이드스캔소나의 수중조사를 위한 장비운용 시 발생하는 영상의 흔들림을 해결하기 위해, 흔들림 보정센서를 부착하고 흔들림 보정 알고리즘을 개발하여 흔들림보정이 가능하도록 프로그램을 보완하였다. 또한, 소나 데이터 해상도의 향상 정도를 검증하기 위하여, 현장조사를 통해 흔들림 보정 전 후 음파영상을 수집하고 손상 의심부에 잠수사를 투입하여 실제 손상길이 및 수심을 측정하여 취득한 음파영상 데이터의 분석을 수행하였다. 본 연구는 향후 사이드스캔소나를 활용한 구조물의 수중부 및 하상면 음파영상 촬영기법의 발전에 기여할 것으로 판단된다. In the case of the side scan sonar operated by the towing method, the underwater structure electric jig was developed because there is a difficulty in the cross-sectional survey that the user wants when conducting the survey. However, in the case of the sound wave photographing method using the electric jig, since the boat and the sonar behaves as one body, data distortion has occurred due to various problems according to working environment, such as, the rolling phenomenon of the boat due to the wave and the fluctuation of the sonic image due to the inoperability of the boat driver. Therefore, in order to solve the image blurring caused by the operation of the equipment for underwater survey of the existing side scan sonar, in this research, the program was supplemented to enable the shake correction by attaching the shake correction sensor and developing the shake correction algorithm. In order to verify the improvement of the sonar data resolution, the sonic images before and after the shake correction were collected through on-site investigation and the analysis of the sonic image data acquired by a diver measuring the actual damage length and depth. This study is expected to contribute to the development of sonar imaging technique of the underwater surface of the structure and bed surface of the sea or a river using the side scan sonar in the future.
식품의약품안전처 SESSION : GC-MS/MS를 이용한 현미 중 112 잔류농약의 다성분 동시 분석
이종화 ( Jong Hwa Lee ),차경훈 ( Kyung Hoon Cha ),이진범 ( Jin Beum Lee ),김병준 ( Byung Joon Kim ),이혜리 ( Hyeri Lee ),김은혜 ( Eun Hye Kim ),김수희 ( Su Hee Kim ),김정한 ( Jeong Han Kim ) 한국환경농학회 2013 한국환경농학회 학술대회집 Vol.2013 No.-
A multi-residue method for simultaneous analysis of 112 pesticides by GC-MS/MS (gas chromatography-tandem triple quadrupole mass spectrometry) in rice was developed. Three sample preparation methods such as QuEChERS (quick, easy, cheap, effective, rugged and safe), KFDA method and PLS (Japanese positive list system method) were applied to rice for analysis pesticide. Recovery, matrix effect and process efficiency of the sample preparation methods were compared. For GC-MS/MS, two MRM (multiple reaction monitoring) transitions per compound were optimized to increase confidence in identification. Correlation coefficients (R2) of calibration curves and LOQ (limit of quantitation) were in range >0.99 and 0.001-0.050 mg kg-1, respectively. Quantitation was determined using matrix matched calibration curves at concentration ranging from 0.002 mg kg-1 to 0.2 mg kg-1. To evaluate performance of the each sample preparation method, recovery tests were carried out on rice at spiking levels 0.01 and 0.05 mg kg-1. A most of compounds could be recovered with QuEChERS method as well as with the PLS method, while the KFDA method could not recover 10 pesticides. Average recovery in the range of 70-120% (RSD ≤20%) at the lower fortification level of 0.01 mg kg-1 were approximately 91.1% (QuEChERS), 89.3% (KFDA method) and 83.0% (PLS method) of 112 compounds. At higher fortification levels of 0.05 mg kg-1, approximately 99.1% (QuEChERS), 45.5% (KFDA method) and 93.8% (PLS method) of 112pesticides were in the range of 70-120% (RSD ≤20%) which is a validation criteria of European Union in single residue analysis. The QuEChERS method was found to produce least matrix effect while the KFDA method produced highest. In aspect on process efficiency, the QuEChERS method demonstrated the advantage of less-time, less-reagents, and less-labor consuming in sample preparation procedure, and gave best recovery rates.
대장암 세포주에서 genistein과 daidzein의 병합처리에 의한 상승적인 세포독성 효과
손성민(Seong-Min Son),임승현(Seung-Hyun Lim),김효림(Hyo-Rim Kim),김민정(Min-Jeong Kim),김태완(Taewan Kim),이종화(Jong-Hwa Lee),김종식(Jong-Sik Kim) 한국생명과학회 2009 생명과학회지 Vol.19 No.9
콩의 대표적인 이소플라본인 genistein과 daidzein에 의해 암세포 생존율에 미치는 영향을 확인하기 위하여, HCT116 세포주에 genistein과 daidzein을 농도 의존적으로 처리하였다. Genistein은 처리한 농도 의존적으로 암세포 생존율을 감소시켰으며, 이에 반해 daidzein은 세포생존율에 큰 변화를 보여주지는 못하였다. 이전의 마이크로어레이 실험 결과에 의하면, 50 μM의 genistein에 의해 2배 이상 증가되는 유전자 71개, 2배 이상 감소되는 유전자 64개가 검색되었다. 이중 3개의 유전자(DKK-1, ATF3 그리고 NAG-1)를 선택하여, 마이크로어레이 실험 결과를 검증하기 위하여 RT-PCR을 수행하였다. RT-PCR 결과 마이크로어레이 결과와 모두 일치함을 증명하였다. 한편, genistein과 daidzein에 의한 병합처리에 의해 암세포생존에 미치는 영향을 확인하였다. 그 결과 병합처리에 의한 상승적인 세포독성 효과를 확인하였다. RT-PCR과 real-time PCR의 결과 genistein과 daidzein의 병합처리에 의해 항암유전자인 NAG-1 유전자가 상승적으로 발현이 증가됨을 확인하였다. 이러한 결과는 이소플라본뿐만 아니라 대두제품에 의한 암 화학예방법의 기전을 이해하는 도움을 줄 것으로 생각된다. To investigate whether isoflavone genistein and daidzein could affect cancer cell viability, human colorectal HCT116 cells were incubated with genistein or daidzein in a dose-dependent manner. Genistein decreased cancer cell viability in a dose-dependent manner, whereas daidzein did not show dramatic cytotoxic effects. We also found that 71 genes were up-regulated more than 2-fold, whereas 64 genes were down-regulated more than 2-fold with 24 hr of 50 μM genistein treatment by our previous microarray data. Among the up-regulated genes, we selected 3 genes (DKK1, ATF3 and NAG-1) and performed RT-PCR to confirm microarray data. The results of RT-PCR were highly correlated with those of the microarray experiment. In addition, we investigated whether a combination treatment of genistein and daidzein could affect cancer cell viability. Surprisingly, the combination treatment did show synergistic cytotoxic effects detected by MTS assay. The results of RT-PCR and real-time PCR indicate that a combination of genistein and daidzein can synergistically induce NAG-1 expression in HCT116 cells. This result implies that NAG-1 induction is highly associated with synergistic cytotoxic effects induced by a combination treatment of genistein and daidzein. Overall, these results may provide a clue in explaining the anti- cancer activity of soy bean in human colorectal cancer.
광섬유센서를 이용한 잔교식 항만구조물 건전성 모니터링에 대한 연구
이종화 ( Yi Jong-hwa ),김영석 ( Kim Young-seok ) 한국구조물진단유지관리공학회 2022 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.26 No.2
센서를 이용한 항만구조물의 계측관리는 전기식센서에 비해 부식에 대한 저항이 뛰어난 광섬유센서를 이용하여 수행하고 있다. 계측시에는 계측값의 임계치를 선정하고 임계치 초과 여부를 확인하여 항만구조물의 거동을 확인한다. 항만구조물의 계측관리를 위해 2006년 준공된 임의의 잔교식 항만구조물을 광섬유센서를 이용한 계측관리를 수행 몇 가지 문제점을 도출하였다. 이를 개선하여 1979년 준공된, OO부두의 구조해석을 통해 센서의 위치를 선정하였으며, 이를 기반으로 임계치 선정 및 계측을 수행하였다. 본 연구를 통해 항만구조물에 대한 계측 및 유지관리가 즉각적으로 이루어지고, 이에 따른 항만구조물의 사고 예방 등이 가능할 것으로 기대된다.