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학습전략훈련이 학습장애자의 수학 학업성취, 자기조정, 충동성 및 자기효능감에 미치는 효과
이종삼 한국교육학회 1995 敎育學硏究 Vol.33 No.3
본 연구는 학습장애자를 위한 학습전략훈련이 그들의 수학 학업성취, 자기조정, 충동성 및 자기효능감에 미치는 효과를 분석할 목적으로 수행되었다. 연구대상은 학습장애자 선정기준에 따라 중학교 학습장애자 92명을 표집하여 실험집단과 통제집단으로 나누었고, 이들 집단을 주의결함 輕ㆍ重에 따라 각각 2개 집단으로 나눈 후 본 연구에서 구조화한 학습전략 교수모형을 투입하여 학교현장에서 실험을 실시하였다. 연구결과 학습전략훈련은 학습장애자의 수학 학업성취를 크게 향상시켰으며, 자기조정학습전략 사용빈도를 증가시켰다. 또한 학습전략훈련은 학습장애자의 충동성을 감소시켰을 뿐만 아니라, 수학에 대한 자기효능감을 향상시키고 있음이 확인되었다.
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이종삼,박영복 한국미생물학회 1981 미생물학회지 Vol.19 No.1
1.Each cells homogenized from Undaria were reacted in reaction micture to persue the phosphate metabolism in Undaria cell. Aliquots of the cells were taken out at the begin-ning and at intervals during the reaction, and analyzed for the content of total-P in various fractions of the cell constituents. 2.The P-contents in fraction of polyphosphate "B" decreased remarkably, while that in fraction of RNA polyphosphate "C" showed slow increase. 3.As well as in Chlorella cells, inorganic phosphates in DNA-P, protein-P, and lipid-P were transferred from polyphosphate, RNA-P turnovered from inorganic phosphate that is in cytoplasm, and RNA polyphosphate complex from polyphosphate, and it was suggested that inorganic phosphates in polyphosphate "B" could transformed into polyphosphate "A" & "C", and polyphosphate "C" into polyphosphate "A".
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효모의 배양시기에 따른 인산화합물의 합성 및 효흡능에 미치는 탄수원의 영향
이종삼,조선의,이기성,신홍기,최영길 한국미생물학회 1981 미생물학회지 Vol.19 No.2
The growth rate of yeast population (Saccharomyces uvarum) cultivated in the Knopp's modified medium (plus various carbon sources) appeared the highest value when the Knopp's minimal medium was treated to 1.5% with disaccharide such as maltose and sucrose. Also the treatment of lactose and raffinose resulted in polulation growth as to the population size in case of maltose and sucrose. However, the gorwth of yeast was not occurred at all when a polysaccharide, such as inulin, was added as carbon source. The growth from of yeast population in Knopp's modified medium are characterized by the fact that log phase continued 100hrs after inoculation and that stationary state phase appeared in general 250hrs after inoculation. Applying the various carbon sources to respiration substrate for yeast cell, the respiration rate of yeast showed the highest value in treatment of maltose and followed in order of raffinose, lactose, glucose, and sucrose. Determined the amount of poly-phosphate and turn over pathway of poly-phosphate according to culture phase of yeast, it is revealed that the yeast synthesized 3 types of poly phosphate (poly-P A,B, and C) and postulated that turn over pathway of poly-phosphate as follows ; Inorganic phosphate is converted into each kind of polyphosphates, and then one part of poly-P-C is converted into poly-P-B, the rest poly-p-C and poly-P-B are converted into poly-P-A. The synthesized poly-phosphate is considered to have a role as energy pool utilizing to synthesis of cellular organic materials. Of the 13 carbon sources used in this experiment, the useful carbon sources for biosynthesis of poly-phosphate and cellular organic materials are confirmed as disaccharide (maltose and sucrose) as well as glucose. Protein synthesis in yeast cell showed the two peaks on 6th and 8th day after inoculation ; nucleic acid on 2nd day (48hrs), carbohydrates on 2nd day (48hrs), and phospholipid on 2nd and 8th day after inoculation, respectively.
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효모(Saccharomyces uvarum)의 인산대사 및 체물질 생합성에 미치는 pH의 효과
이종삼,허정희 誠信女子大學校 1992 硏究論文集 Vol.32 No.-
효모세포(Soccharoyces uvarum)의 생육시기에 따른 무기인산의 전환과정과 유기물합성 및 생장에 미치는 pH효과를 조사하기 위하여 knopp's배지를 pH2, pH5, pH8로 나누어 각 배지에 세포를 접종하여 7일간 배양하면서 세포의 생장률 및 구성물질의 함량을 비교 분석하였다. 효모세포는 pH5에서 가장 높은 생장률을 나타내어 배양기간 동안 인지질, protein bounding phosphate 및 DNA합성에 필요한 무기인산은 인산의 저장소이며 에너지원인 무기폴리인산으로부터 인산기로의 전환이 정상적으로 진행되는 추세를 보였으며 pH8도 비슷한 경향을 나타내었다. pH2에서는 강한 산성으로 인한 효소의 불활성으로 물질대사에 있어서 조절자(regulator)역할을 하는 무기폴리인산의 전환과정이 저해되는 것으로 나타났다. 그러나 RNA poly-p complex로부터 DNA나 protein으로 무기인산의 전환과정은 pH변화에 따른 별다른 저해현상이 보이지 않았다. 효모세포의 최적 pH생장조건은 약산성이며, 알카리 조건보다 강한 산성조건에서 저해작용이 큰 것으로 나타났다. To investigate the effect of pH on the phosphorylation and biosynthesis of organic compounds, 0.5ml of the yeast cell that were measured in the packed cell volume was cultured under the three separated conditions in knopp's media for 7 days. After the yeast cells were harvested at 2days intervals during the culture, the growth rate of the cell was observed by counting the cell number with the haematocytometer and the absorbancy of the each media by the spectrophotometer (660nm). The fractionation of the cell were prepared for tracing of contents of inor-ganic polyphosphoric acid and organic compounds. As a result, pH5 showed very remarkable growth rate of the cell and the strong acid (pH2) was more inactivated than alkali (pH8). Inorganic polyphosphoric acid which were polyphosphoric acid's storage and energy sourcein the yeast cell at pH5 was entirly decreased during the culture. On the other hand, the amounts of the phosphoric acid compounds and the organic compounds was increased and activated in transferation by metabolism. However, there were not a remarkable change at pH2 beacause it was interpreted as controlled the function of inorganic polyphosphoric acid which enacted as a regu-lation in metabolism for inactivated enzyme by the strong acid.
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Saccharomyces uvarum의 배양시기에 따른 여러가지 인산화합물의 함량에 미치는 IAA의 효과
이종삼,조선희 한국미생물학회 1981 미생물학회지 Vol.19 No.2
In order to interpret the effect of IAA on the phosphate metabolism and biosynthesis of organic compounds, Saccharomyces uvarum were cultured in the media treated with various concnetration of IAA $(10^{-3}M,\;10^{-5}M,\;10^{-7}M)$. Sampling at the beginning and intervals of culture, yeast cells fractionated were traced the contents of inorganic phosphate and organic compounds of various fractions. 1. Growth of Saccharomyces uvarum were enhanced by IAA $(10^{-3}M,\;10^{-5}M)$ and phosphate contents in DNA and RNA fractions treated with IAA were accelerated 2.3 times and 2 times in comparison with those of control. 2. Amounts of poly-P"A" and poly-p"B" were increased but poly-P"C" decreased during the culture. Therefore, it is considered that poly-P"C" play on most important role as a phosphate pool. 3. It is suggested that because phosphate contents in DNA, protein and lipid fractions increased, inorganic phosphates required phosphates required RNA were transferred from phosphates in cytoplasm, because these increased slowly during the culture. 4. Alkali-labile protein were accelerated by IAA and alkali stable protein only were inhibiction were enhanced by IAA while, ethanol : ether soluble fraction was induced by $10^{-7}M$ IAA in comparison with those control.X> IAA in comparison with those control.
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