http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 이민전(Min Jun Lee) (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
-
면역결핍바이러스 인테그라제 억제제로서 Baicalein과 Baicalin
이민전(Min Jun Lee),김미라(Mi Ra Kim),이용섭(Yong Sup Lee),신차균(Cha Gyun Shin) 대한약학회 2003 약학회지 Vol.47 No.1
Baicalein and baicalin are flavonoid compounds isolated from medicinal herb Scutellaria baicalensis Georgi (Labiatae) and have been known to possess antiviral activities. In the present study; we investigated the in vitro effects of baicalein and baicalin on the three distinctive enzymatic activities of the human immunodeficlency virus type-1 (HIV-1) integrase - endonucleolytic, integration, and disintegration activities. Both compounds inhibited the three enzymatic activities in a dose-dependent manner The 50% inhibitory concentrations of baicalein and baicalin for endonucleolytic activities of HIV-1 integrase were 4.4 +/- 3.3 and 25.9 +/- 4.0μM, respectivel. In general, baicalein exhibited nearly 6- to 10-fold stronger inhibition than baicalin for the three enzymatic activities. These data demonstrate that baicalein or baicalin can be used as a leading compound to develop anti-AIDS chemotherapeutic agents targeting to the HIV-1 integrase.
-
Oct4 단백질의 DNA - 결합 영역에 융합된 HIV - 1 integrase
이민전,서진욱,김미라,신차균 中央大學校 遺傳工學硏究所 2002 遺傳工學硏究論集 Vol.15 No.1
Human immunodeficience virus type-1 (HIV-1) integrase (IN) mediates integration of viral DNA into the host cell genome which is an essential step during the life cycle of retroviruses. HIV-1 IN shows four enzymatic activities in vitro: non-specific DNA- binding, endonucleolytic (3'-processing), integration (strand transfer), and disintegration activities. Oct4 as a transcription factor recognizes and binds the specific sequences containing 5' -ATGCAAAT-3'. In order to study the specific binding and enzymatic activities of the integrase protein, we constructed the four different fusion proteins (OctIN1, OctIN2, INKOct1, INKOct1) using different combination of HIV-1 IN and Oct4 protein. The fusion proteins were overexpressed in Escherichia coli and purified by Ni-NTA and SP-sepharose columns. Analysis of the enzymatic activities showed that all the four fusion proteins has detectable endonucleolytic and integration activities, suggesting that the fusion proteins can be used to study derected integration and sequence specific DNA-binding of the integrase fusion protein.
-
상피종양의 keratin 생성능에 대한 전자현미경적 연구
이길선,이명주,장정수,이민전,노광을,서재홍 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1
Keratins are characteristically present in all keratinizing and nonkeratinizing epithelial cells and their neoplasms. They consist of at least 19 different polypeptides ranging from 40 to 69 kilodaltons, which are developmentally related to specific epithelial cell types. Keratins of high and intermediate molecular weights are readily demonstrated in squamous cell carcinomas, which are characteristically rich in tonofilaments and tonofilament-desmosome complexes. Keratins of low molecular weights are expressed in adenocarcinoma including renal cell carinoma, hepatocellular carcinoma, small cell carcinoma, and carcinoid tumor. These tumors do not reveal any tonofilaments and sacrcely show discrete filaments on electron microscopy. An immunoperoxidase technique employing antibody to keratin was used to study distribution and staining pattern of keratin filaments in benign and malignant epithelial tumors (20 squamous cell carcinomas, 20 gastric adenocarcinomas, 20 transitional cell carcinomas and 15 non-neoplastic epithelia). These immunohistochemical results were compared with ultrastructural features of neoplastic diagnosis of squamous cell carcinoma, adenocarcinoma and transitional cell carcinoma, and estimation of degree of differentiation. The results are as follows : 1. The squamous epithelium, glandular epithelium and transitional epithelium disclosed the positive reaction for keratin. 2. Squamous cell carcinoma and transitional cell carcinoma exhibited positive reaction for keratin. 3. Gastric adenocarcinoma showed uniformly negative or weakly positive reaction for keratin. 4. On electron microscopy, heavy bundles of tonofilaments and desmosomes were more frequently encountered in well differentiated squamous cell carcinoma, In poorly differentiated squamous cell carcinoma, a few tonofibrils and desmosome were noted. 5. In the adenocarcinomas, the intermediate filaments were arranged randomly as nonaggregated, short filaments spread throughout the cytoplasm, although occasionally they formed a perinuclear whorl. These filaments did not become aggregated to form tonofilaments. 6. On the immunogold labeling for keratin of low molecular weight areas in gastric adenocinoma, in tense labeling of intermediate filaments for keratin was noted. From the above result, ultrastructural and immunohistochemical study on malignant epithelial cell were useful in differential diagnosis of squamous cell carcinoma, adenocarcinoma and transitional cell carcinoma, and estimation of degree of differentiation. It was also proved that immunogold labeling technique was especially valuable for diagnosis of vague case which showed negative or weakly positive reaction in immunohistochemical stain.
-
자궁의 악성 혼합성 뮬러 종양 : 1예 보고 report of a case
기근홍,노광을,이민전 조선대학교 1993 The Medical Journal of Chosun University Vol.18 No.2
Malignant mixed mullerian tumor is rare tumor of the uterus. This tumor derived from mullerian mesoderm, but their histogenesis is still controversial. We report a case of homologous type of malignant mixed mullerian tumor of the uterus in 64 year-old female. The tumor composed of adencocarcinoma and leiomyosarcoma. After radical hysterectomy, this tumor is metastasize to lung 5 months later.
ScienceON
스콜라연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
