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이대일(Dae Il Lee),최병철(Byung Chul Choi),박영민(Youngmin Park) 한국전산유체공학회 2011 한국전산유체공학회 학술대회논문집 Vol.2011 No.5
To investigate aerodynamic performance of high-lift devices, 2D design is the base of the success of high-lift system design for transport aircraft, which can shorten the periods of three-dimensional design and analysis. For the simulation coupled viscous and inviscous euler method (MSES) is used. In this parametric study, Gap and Overlap which can define position of flap is used as design variables and we investigate relation between angle of attack and flap position for lift enhancement.
Study of Human Insulin : I. Synthesis and Cloning of Genes for Human Insulin A and Chains
박병철,임향숙,김명희,이대실,Park, Byung-Chul,Lim, Hyang-Sook,Kim, Myoung-Hee,Lee, Dae-Sil 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.3
인슈린을 박테리아에서 발현시키기 위해 인체 인슐린 A와 B 사슬에 해당하는 유전자를 설계하였다. 화학적 합성, 생화학적 조립, 유전자 재조합상 용이성 등이 고려된 설계에는 각 인슈린 사슬이 11개의 유전자 절편으로 구성되도록 하였다. 계획된 유전자 절편은 phosphite triester 합성법으로 합성하였으며, 인슐린의 A와 B 사슬의 합성유전자를 건설하기 위해서 합성된 유전자 절편을 효소적인 방법으로 결합하였고, 이어서 대장균에 클로닝 하였다. 클론들은 계획한대로 인슐린 유전자가 대장균에 도입되었음을 유전자 서열 결정에 의해 확인하였다. Total synthesis of human insulin A and B chain genes were designed for the expression through bacterial system. Eleven individual oligonucleotides corresponding to insulin A and B chains were chemically synthesized by the phosphite triester method on solid support. The enzymatic joining of the oligonucleotides were employed to give the synthetic human insulin A and B chain genes, which ware separately cloned into the bacterium Escherichia coli using plasmid, pUC19. The cloned DNAs were shown to have the correct nucleotide sequence as designed.
인체 인슈린 연구 . 1 . 인체 인슈린 A 와 B 사슬의 유전자합성과 클로닝
박병철,임향숙,김명희,이대실 ( Byung Chul Park,Hyang Sook Lim,Myoung Hee Kim,Dae - Sil Lee ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.3
Total synthesis of human insulin A and B chain genes were designed for the expression through bacterial system. Eleven individual oligonucleotides corresponding to insulin A and B chains were chemically synthesized by the phosphite triester method on solid support. The enzymatic joining of the oligonucleotides were employed to give the synthetic human insulin A and B chain genes, which ware separately cloned into the bacterium Escherichia coli using plasmid, pUC19. The cloned DNAs were shown to have the correct nucleotide sequence as designed.