활천을 여는 글 : 여교역자회전국연합회 신임회장 - 이군자 전도사 ( mini interview )

이군자 기독교대한성결교회 활천사 2002 활천 Vol.587 No.-

日本紀行 : 이웃나라 일본의 인상

이군자 경기대학 관광학회 1967 觀光學報 Vol.1 No.-

Mouse 신장으로부터 Ornithine Decarboxylase의 정제

이군자,조영동 한국생화학회 1992 BMB Reports Vol.25 No.5

자성 mouse의 피하층에 testosterone을 투여하여 ornithine decarboxylase의 활성을 증가시킨 후, 신장으로부터 ornithine decarboxylase를 황산암모늄 분별침전, DEAE-sephacel chromatography, hydroxylapatite chromatography, pyridoxamine-5'-phosphate agarose affinity chromatography를 이용하여 분리 정제하였다. 이 효소의 분자량은 SDS-polyacrylamide gel electrophoresis, Sephacryl S-200 gel chromatography와 ornithine decarboxylase의 효소활성 억제제인 14C-DFMO 을 효소와 결합시켜 얻은 autoradiography를 이용하여 52,000 dalton인 동일한 subunit으로 구성된 dimer임을 알 수 있었다. 정제된 효소의 ornithine에 대한 K_(m) 값과 V_(max) 값은 각각 167㎛과 5 nmol CO_(2)/h로 나타났고, pyridoxal-5-phosphate에 대한 K_(m) 값과 V_(max) 값은 각각 0.142㎛과 2.4nmol CO_(2)/h로 나타났다. 효소의 최적 pH 는 7.0, 최적 온도는 37℃이었으며, 정제된 ornithine decarboxylase는 polyamines(putrescine, spermidine, spermine)에 의해 효소 활성이 영향을 받지 않았다. 본 실험에서는 ornithine decarboxylase의 분리과정 중에서 ornithine decarboxylase 활성을 안정화시키는 물질을 발견하였다. Ornithine decarbosylase (ODC) was purified to homogenity from testosterone treated mice kidney by ammonium sulfate fractionation, DEAE-Sephacel chromatography, hydroxylapatite chromatography, pyridoxamine-5-phosphate-agarose affinity chromatography. The specific activity of ODC was 14,000 U/㎎, and purification fold was 1,180. The enzyme has a Mr. of about 110,000 dalton and is a dimer of subunit Mr. 52,000. The K_(m), for L-ornithine was 167㎛ and for pyridoxamine phosphate, 142 nM. ODC has an optimum pH of 7.0 and optimum temperature of 37℃. The purified enzyme was not inhibited by polyamine (putrescine, spermidine and spermine). During purification process, ODC stabilizing material was found.

최루가스(o-Chlorobenzylidene Malononitrile)가 흰쥐의 각막과 결막에 미치는 영향

이군자 서울보건대학 1995 서울보건대학 부설 한국보건과학연구소 논문집 Vol.2 No.1

Two groups of rats were exposed to the o-chlorobenzylidine malononitrile(CS) being used in the control of riots, and sacrificed to examine histological and histochemical changes of the cornea and conjunctiva. 1. In animals exposed to the CS gas (50mg/min./㎥) for 20min., reversible histological changes were observed in the cornea. Epithelial and endothelial Na,^(+) K^(+) -ATPase activity were reduced, wing cells of the epithelium were transformed to the squamous shape and mitotic activity occurred actively in the basal layer. 2. In animals exposed repeatedly to the CS gas (50mg/min./㎥) for 20min. once a day for 10 days, Na^(+), K^(+)-ATPase, Mg^(2+)-ATPase and alkaline phosphatase activities of the corneal epithelium and endothelium were reduced. And there was epithelial shrinkage, irregular basement membrane and irregularly arranged collagen fibers were also observed in the stroma. 3. In animals exposed to the CS gas (50mg/min./㎥) for 20min., reversible histological changes were observed in the conjunctiva. Mucin secreting activity of the goblet cells was reduced and lymphocytes, eosinophils were infiltrated. 4. In animals exposed repeatedly to the CS gas (50mg/min./㎥) for 20min. once a day for 10 days, irreversible changes were observed. Desquamation with oedema and shrinkage of the epithelial cells, and loss of some basement membrane were observed. Mucin secreting activity of the goblet cells was reduced and lymphocytes, eosinophils were infiltrated significantly. As a result when eyes are temporally exposed to the CS gas reversible degenerative changes, desquamation with oedema of the epithelium and infiltration are produced. And when eyes are repeatedly exposed to the CS gas histopathologic changes such as desquamation with oedema of the epithelium, infiltration, and reduced mucin secreting activity are occurred.

태생기 및 신생기 흰쥐 간장에서의 Putrescine 대사에 관한 연구

이군자 서울保健大學 1992 論文集 Vol.12 No.1

한국인에서 정면 얼굴형태와 눈의 위치에 관한 계측적 연구

이군자,마기중,안동춘,이동희 대한시과학회 1999 대한시과학회지 Vol.1 No.1

머리와 얼굴의 체질인류학적 특징은 환경적 요인에 의해 크게 달라지지 않으며 인 종과 민족을 구별할 수 있는 특정으로 눈의 위치는 눈의 일차 건강진단에 중요한 요 소로 작용한다. 얼굴형태와 눈의 위치는 나이, 성별, 인종에 따라 달라지므로 나이. 성별, 인종에 따라 해부학적 자료가 계측되어야 한다. 따라서 본 연구에서는 안와질 훤. 안외상. 전선질환이 없는 18~33세 사이의 성인 689 명(남자 325 명, 여자 364 명)을 대상으로 한국인의 얼굴형태와 눈의 위치될 파악할 수 있는 여러 계측치를 측정하였 고 각각의 셰측치 상호간의 상관관계를 추적하였으며 성별 및 인종에 따라 계측자료 플 비교 푼석하였다. 한국인의 얼굴형태와 눈의 위치는 성별에 따라 통계화적으로 뚜 렷한 차이가 나타났으며, 눈의 위치는 얼끊형꽤와 서로 상관성이 있음을 알 수 있었 고. 다른 인종파 뚜렷한 차이플 나타내었다 본 연구결과는 한국인의 얼굴형태와 눈 의 위치에 적합한 안경테 및 안경렌즈 제-죠 등을 위한 산업현장에서 활용될 수 있을 것으로 기대된다. The anatomical structures of the head and face are not influenced by environmental factors but vary from one race to the other. The facial and ocular characteristics may be enough to diagnose a particular syndrome in primary eye care. So the facial and ocular dimensions have to be measured for a given age. population group and sex. This study was therefore undertaken to determine normal values of facial and ocular dimensions in the 689 adult Korean free from systemic and ocular diseases or anomalies. All measurements were significantly different between sexes and among population groups and ocular position showed a significant correlation with facial values. We expect that this database of ophthalmic anthropology will be useful for the design of spectacJes and optical lenses for Korean.

운동프로그램이 중년비만여성의 체구성, 체력 및 지질대사에 미치는 효과

이군자 한국간호과학회 2005 Journal of Korean Academy of Nursing Vol.35 No.8

Purpose: This study was to examine the effects of an exercise program for middle-aged obese women. Method: The exercise program combined folk dance and resistance training. The subjects group consisted of 85 middle-aged obese women between 40 and 60 years of age. Three 8 week sessions consisted of a 55-80% maximum heart rate (MHR) exercise for 60-90 minutes a day and 3 times a week from March to November, 2004. Data was collected through a pre- and post-exercise test before and after each session. Data was collected with Inbody, dynamometer and blood. This data was analyzed by descriptive statistics, and a paired t-test with an SPSS/PC(10.0 version) program. Results: There were significant positive changes in body weight, body fat mass, body mass index, percent body fat, muscle strength, muscle endurance, flexibility, and balance quality, but no significant positive changes in skeletal mass, fat free mass, percent abdominal fat(waist-hip ratio), visceral fat area, agility, total cholesterol, high density lipoprotein cholesterol, or triglycerides. Conclusion: This study showed that an exercise program has partially positive effects for middle-aged obese women. The results of this study show that exercise at community health centers should continue for middle-aged obese women's health.

운동프로그램이 성인여성의 체력, 심폐기능 및 생활만족도에 미치는 효과

이군자 지역사회간호학회 2005 지역사회간호학회지 Vol.16 No.2

The Effects of an Exercise Program on Physical Fitness, Cardiopulmonary for Adult Women Purpose : This study was to examine the effects of an exercise program on physicalfitness, cardiopulmonary function and lifesatisfaction for adult women. Method : Theexercise program combined dance and resistancetraining. The subject group consisted of 114 women aged between 33 and 60. Three 8-week sessions consisted of 55-80% HR max for 60-90 minutes a day and 3 times a week from March to November, 2004. Data were collected through pre- and post-exercise tests before and after each session. Data were collected with dynamometer, sphygmomamometer, spirometer and structured questionnaires. Data were analyzed employing descriptive statistics and paired t-test with SPSS/PC(10.0version) program. Results: There were significantly positive changes in muscle strength, flexibility, balance quality, forced vital capacity and life satisfaction, but no significantly positive change in agility and blood pressure. Conclusion : This study showed that an exercise program has partially positive effects for adult women. The result of this study suggest that there should be programs of continuous exercise at community health centers for adult women's health.

흰쥐 태반의 Transamidinase 촉매반응에 관여하는 아미노산 잔기의 화학변형

이군자,조영동 한국생화학회 1993 BMB Reports Vol.26 No.8

흰쥐 태반으로부터 transamidinase를 정제한 후 화학변형의 방법을 이용하여 이 효소의 활성부위에 존재하는 아미노산 잔기를 확인하였다. Transamidinase는 sulfhydryl기와 특이하게 반응하는 N-ethylmaleimide, 5'-dithiobis(2-nitrobenzoate)에 의하여 빠르게 활성이 감소되었으며, N-ethylmaleimide에 의한 비활성 반응은 pseudo first-order kinetics로 나타났고 2차 반응속도 상수는 904M^(-1)·min^(-1) 이였으며 반응차수는 0.8이었다. N-ethylmaleimide에 의한 효소의 비활성은 가역반응으로 dithiothreitol에 의하여 활성이 회복되었으며, 기질인 L-arginine에 의하여 보호효과를 나타내었다. 이 효소는 serine과 특이하게 반응하는 diisopropylflurophosphate에 의해서도 비활성되었으며 이 때의 2차 반응속도 상수는 74M^(-l)·min^(-1), 반응차수는 1.1이었다. DFP에 의한 효소의 비활성은 기질인 L-arginine과 glycine에 의하여 모두 보호효과를 나타내었다. 이러한 결과로부터 cysteine과 serine 잔기가 각각 1개씩 이 효소의 활성에 관여할 것으로 추정된다. We examined the chemical modification of the purified rat placenta transamidinase. The enzyme was inactivated by sulfhydryl group specific modification reagents such as NEM and DTNB. The enzyme was turned out to be inactivated by NEM rapidly and the reaction followed pseudo first-order kinetics. The second-order rate constant and reaction order with respect with NEM were 904M^(-1)·min^(-1) and 0.8, respectively. Inactivation by the NEM was restored to almost orginal activity by DTT and was protected by the L-arginine. The transamidiase was also inactivated by the DFP. The second-order rate constant and the reaction order with respect to DFP were 74 M^(-1)·min^(-1) and 1.1, respectively. L-arginine and glycine provided almost complete protection against inactivation by DFP. Such cumulative results suggest that cysteine and serine residues seems to be involved in enzyme activity.

금연 상담프로그램을 통한 금연이 금연 성공자의 호기 CO농도, BMI, 혈압, 간기능 및 지질대사에 미치는 영향

이군자,이명희 지역사회간호학회 2008 지역사회간호학회지 Vol.19 No.2

Purpose: This study was performed in order to examine the effects of an smoking cessation counselling program for smoking cessation success. Method: Among a total of 468 persons who had ceased from smoking for 6-months and had visited the smoking cessation clinic of a public health center from January 2nd to December 31th in 2006, 61 in all who had a negative reaction in the urine nicotine check were selected for this study. Collected data were expiratory CO concentration, BMI, blood pressure, liver function, and lipid metabolism. These data were analyzed by descriptive statistics, repeated measured ANOVA and paired t-test with the SPSS/PC(Version 12.0) program. Result: There were significant changes in expiratory CO concentration, SBP, DBP, AST, ALT, and TG, but not in BMI, γ-GTP, TC, HDL-C, and LDL-C. Conclusion: This study showed that smoking cessation through a smoking cessation counselling program has partially positive effects for smoking cessation success. The results of this study show that the smoking cessation counselling program at the smoking cessation clinic of a public health center should be continued for smoking cessation success.