토끼의 착상기간중 난소홀몬과 난소홀몬 수용체에 관한 연구

윤미정,유경자,조혜성 대한내분비학회 1987 Endocrinology and metabolism Vol.2 No.2

The present study was undertaken to understand the mechanism by which implantation takes place by determining progesterone and estradiol concentrations in serum and their receptors in uterine tissues, thereby correlating hormone levels with their receptor numbers, during the implantation period in rabbits. The results obtained were as follows. 1) Serum estradiol concentrations were not changed from days 1 to 12 whereas progesterone levels were gradually increased, reachingg the maximum on day 7. 2) Kds for cytosolic and nuclear estrogen receptors were 0.41 nM and 0.26 nM while Kds for cytosolic and nuclear progesterone receptors were 1.23 nM and 0.63 nM in uterine tissues on days 6-8. 3) Concentrations of cytosolic and nuclear estrogen receptors and cytosolic progesterone receptor were decreased on days 6-8 compared to those from nonpregnant rabbits whereas nuclear progesterone receptor levels were slightly increased. 4) Cytosolic estrogen receptor levels in the implant sites were lower than those in the non-implant sites whereas nuclear estrogen receptor levels in the implant sites were greater than those in the non-implant sites. It is, therefore, concluded that concentrations of steroid receptors in uterine tissues as well as estradiol and progesterone in serum were changed during the implantation period in rabbits.

흰쥐 수정란 착상시기에 있어서의 호르몬 농도의 변화와 자궁내막의 구조에 관한 연구

윤미정,손성향,김창미,최임순,Yoon, Mi-Chung,Sohn, Seong-Hyang,Kim, Chang-Mee,Choe, Rim-Soon 한국현미경학회 1993 Applied microscopy Vol.23 No.1

The mechanism by which blastocysts implant to uterine endometrium has not been clearly understood. In the present study, the following question was investigated: how are hormonal levels changed and how is uterine endometrium morphologically changed? Results obtained are as follows: Concentrations of serum estradiol and progesterone were significantly increased on day 4 and 5 of pregnancy. Uterine concentrations of PGE and $PGE_{2a}$ were sharply increased on day 1 and maintained similar concentrations thereafter, reaching the maximum on day 5. Both prostaglandins (PGs) concentrations were gradually decreased thereafter. Furthermore, concentrations of PGs in implant sites were greater than those in non-implant sites. PBR (pontamine blue reaction) in uterine endometrium was positive on day 6 of pregnancy, indicating that vascular permeability was increased. Edema and changes in structure of cell components were pronounced in stroma where PBR was developed. Moreover, these were more prominent in implant sites than non-implant sites. These results suggest that uterine PGs as well as steroid hormones increase during implantation in rats and these hormones might be involved in the process of implantation by modulating vascular permeability and the fine structures of uterine endometrial cells.

cAMP Mediation in Estradiol-induced Uterine Prostaglandin Synthesis During the Delayed Implantation Process in Rats

윤미정,김창미,최임순,유경자,Yoon, Mi-Chung,Kim, Chang-Mee,Choe, Rim-Soon,Ryu, Kyung-Za The Korean Society of Pharmacology 1991 대한약리학잡지 Vol.27 No.2

본 연구에서는 흰쥐의 착상지연을 유도하여 착상기간동안 자궁조직내 prostaglandin (PG) 생합성이 어떠한 인자에 의해서 조절되는가를 관찰하여 다음과 같은 결과를 얻었다. 흰쥐의 착상지연과정동안 estradiol을 처리하면 처리후 4시간만에 자궁조직내의 cAMP의 농도가 급격하게 증가하였다. PGE와 $PGF_2{\alpha}$의 농도는 estradiol을 처리한 후 12시간이 경과하였을때 증가하였으나 $PGF_2{\alpha}$의 증가는 통계적으로 유의하지는 않았다. 또한 indomethacin을 estradiol과 동시에 처리하면 estradiol 처리로 인한 PGE와 $PGF_2{\alpha}$의 농도 증가는 나타나지 않았으나 cAMP 농도는 증가하였다. dbcAMP를 처리하면 자궁내 PGE 및 $PGF_2{\alpha}$의 농도가 증가하기 시작하여 estradiol이 투여시에 비하여 4시간 빨리 8시간후에 최고치에 도달하였으며 phosphodiesterase inhibitor인 theophylline을 전처치하면 estradiol만 투여한 것에 비하여 자궁조직내 PGE 및 $PGF_2{\alpha}$의 농도가 유의하게 증가하였다. 이상의 결과로 보아 흰쥐의 착상지연과정동안 estradiol이 자궁의 prostaglandin 합성을 증가시키며 이러한 증가는 cAMP의 증가를 매개하는 것으로 생각된다. The present study was performed to elucidate the factors which modulate uterine prostaglandin synthesis during the implantation period in rats, by employing delayed implantation model. Administration of estradiol sharply increased uterine cAMP concentration 4 hrs later during the delayed implantation process. Concentrations of uterine PGE and $PGF_2{\alpha}$ were increased at 12 hrs after the estradiol treatment although an increase in $PGF_2{\alpha}$ was not statistically significant. The concomitant treatment of indomethacin with estradiol significantly suppressed estradiol-induced PGE and $PGF_2{\alpha}$ at 12 hrs, while uterine cAMP concentration was not suppressed. The treatment of dbcAMP without estradiol gradually increased uterine PGE and $PGF_2{\alpha}$ showing the maximum 8 hrs later, suggesting that cAMP minics estradiol effect on uterine prostaglandin synthesis during the implantation process. Furthermore, the pretreatment of theophylline, phosphodiesterase inhibitor, induced significantly greater concentrations of uterine PGE and $PGF_2{\alpha}$, compared with estradiol-only treated group. These results suggest that estradiol stimulates uterine prostaglandin synthesis and this process may be mediated by an elevation of cAMP during the delayed implantation process in rats.

W. B. Yeats의 시에 나타난 시인의 역사관

윤미정 동덕여자대학교 영어영문학회 1992 Duet Vol.9 No.-

변연골 흡수가 내측연결 임플란트 매식체의 응력분포에 미치는 영향

윤미정,윤민철,엄태관,허중보,정창모 대한치과보철학회 2012 대한치과보철학회지 Vol.50 No.2

Purpose: The change of the marginal bone around dental implants have significance not only for the functional maintenance but also for the esthetic success of the implant. The purpose of this study was to investigate the load transfer of internal conical joint type implant according to marginal bone resorption by using the three-dimensional finite element analysis model. Materials and methods: The internal conical joint type system was selected as an experimental model. Finite element models of bone/implant/prosthesis complex were constructed. A load of 300 N was applied vertically beside 3 mm of implant axis. Results: The pattern of stress distribution according to marginal bone resorption was similar. The maximum equivalent stress of implant was increase according to marginal bone resorption and the largest maximum equivalent stress was shown at model of 1 mm marginal bone resorption. Although marginal bone loss more than 1mm was occurred increasing of stress, the width of the stress increase was decreasing. Conclusion: According to these results, the exposure of thin neck portion of internal conical joint type implant is most important factor in stress increasing. 연구 목적: 지금까지 성공적인 임플란트 치료를 위해 많은 연구가 진행되어 왔으며, 임플란트 주변 골 흡수 현상에 대한 연구는 매우 관심이 높은 분야 중 하나이다. 이에 본 연구에서는 삼차원 유한요소응력분석을 이용하여 변연골 흡수가 내측연결 임플란트 매식체의 기계적 안정성에 미치는 영향을 간접적으로 확인하고자 하였다. 연구 재료 및 방법: 악골에 식립된 내측연결 형태의 임플란트 매식체에 티타늄 소재의 임플란트 지대주를 지대주 나사로 연결하고 상부에 금합금관을 장착하는 삼차원 유한요소모형을 설계하였다. 0, 1, 2, 3 mm의 변연골 흡수 상태를 적용하고, 교합면 중심에서부터 3 mm 편측에 300 N의 수직 하중을 가하여 임플란트 매식체에 발생하는 최대 주응력을 계산하였다. 결과: 유한요소분석결과 변연골 흡수에 따른 임플란트 매식체의 최대 주응력 분포는 유사한 양상을 보였으며, 임플란트 매식체 상단에서 가장 높은 응력 집중이 나타났다. 최대 주응력은 처음 1 mm 변연골 흡수를 가정하였을 때 가장 크게 증가하였고, 이후 변연골 흡수가 증가할수록 응력은 증가하였지만 응력 증가의 폭은 감소하는 경향을 보였다. 결론: 이러한 결과로부터 내측연결 임플란트에서 매식체 두께가 얇은 경부의 노출은 변연골 흡수로 인한 응력 증가에 가장 큰 원인임을 알 수 있었으며, 매식체의 변형, 균열 및 파절 등의 기계적 실패를 감소시키기 위해서는 이에 대한 외과적, 보철적 고려가 필요할 것으로 생각된다.

한의학적 치료로 호전된 전이성 대장암 환자의 복통에 대한 증례보고

윤미정,김나연,최홍식,김승모,김경순,Yoon, Mi-jung,Kim, Na-yeon,Choi, Hong-sik,Kim, Seung-mo,Kim, Kyung-soon 대한한방내과학회 2021 大韓韓方內科學會誌 Vol.42 No.4

Objectives: The aim of this case report is to present abdominal pain relief in a patient diagnosed with colorectal cancer with several organ metastases. Methods: The patient was treated with herbal medicine, acupuncture, and moxibustion. The effect of the treatment was measured by a numeric rating scale (NRS). Results: The NRS of the patient's abdominal pain was reduced after Korean medicine treatment. Conclusions: This case report suggests that Korean medicine may be effective for treating abdominal pain in cancer patients. However, additional study is needed to confirm interactions between Korean medicine and the control of abdominal pain.

외식업체의 비대면 서비스 품질이 만족과 구매의도에 미치는 영향

윤미정,고윤희 (사)한국조리학회 2020 한국조리학회지 Vol.26 No.6

The purpose of this study is to verify the relationship between the non-face-to-face service quality of catering companies on satisfaction and purchase intention. A total of 400 questionnaires were distributed to consumers in their 20s and 40s who had experience in using non-face-to-face services of catering companies, and 334 valid questionnaires were used for empirical analysis. As a result of this study, first, the reliability, promptness, price competitiveness, and information quality of non-face-to-face service quality of catering companies have a significant positive effect on satisfaction. Second, the verification of Hypothesis 2 showed that the reliability, speed and price competitiveness of the non-face-to-face service quality of the restaurant company had a significant positive effect on the intention of purchase, and that information quality did not affect the intention of purchase. As a result of verification of Hypothesis 3, satisfaction was found to have a significant positive effect on purchase intention. Therefore, in order to increase the quality of non-face-to-face service, it is considered that the customer must be satisfied only when the information quality and reliability of the service are high as well as the more accurate and prompt service delivery.

Fenofibrate regulates obesity and Lipid metabolism with sexual dimorphism

윤미정,정선효,ChristopherJ.Nicol,이형희,한미영,김정재,서윤정,유칠열,오구택 생화학분자생물학회 2002 Experimental and molecular medicine Vol.34 No.6

To determine whether the PPARα agonist fenofi-brate regulates obesity and lipid metabolism with sexual dimorphism, we examined the efects of (WAT) mas, circulating lipids, and the expresion of PPARα target genes in both sexes of high fat diet-fed C57BL/6J mice. Both sexes of mice fed a high-fat diet for 14 weks exhibited increases in body weight, visceral WAT mas, as wel as serum triglycerides and cholesterol, although these ef-fects were more pronounced among males. Feed-ing a high fat diet suplemented with fenofibrate (0.05% w/w) reduced al of these effects signifi-cantly in males except serum cholesterol level. Fe-males on a fenofibrate-enriched high fat diet had ler extent compared to males, but did not exhibit decreases in body weight, WAT mas, and serum cholesterol. Fenofibrate treatment resulted in he-patic induction of PPARα target genes encoding enzymes for faty acid β-oxidation, the magnitudes of which were much higher in males compared to females, as evidenced by results for acyl-CoA oxidase, a first enzyme of the β-oxidation system. These results suggest that observed sexually di-morphic effects on body weight, WAT mass and related elements in the diferential activation of PPARα.

흰쥐 착상시기에 자궁내 난소 홀몬 수용체와 Prostaglandin 및 cAMP 농도변화에 관한 연구

윤미정,유경자,Yoon, M.,Ryu, K. 대한생식의학회 1987 Clinical and Experimental Reproductive Medicine Vol.14 No.1

In the present study, hormonal changes in uterine tissue and circulation were evaluated during the implantation period in rats in order to understand the mechanism by which implantation takes place. The results obtained were as follows. 1. Concentrations of serum estradiol and progesterone were significantly increased on days 4 and 5. 2. Concentration of estrogen receptor reached maximum on day 5 when implantation normally occurred in rats. On the other hand, progesterone receptor was gradually decreased, reaching the lowest on day 5. 3. Uterine PGs and cAMP concentrations were significantly increased on day 5. 4. Uterine PGs and cAMP concentrations in implant sites were significantly greater than those in non-implant sites. It is, therefore, concluded that prostaglandins and cAMP in uterine tissue as well as circulating ovarian steroid hormones were increased during the period of implantation, suggesting that these hormones might be actively involved in the process of implantation in rats.

과학 수업 장면 그리기를 통한 교사와 학생의 수업 이미지 분석

윤미정,최선영,여상인 仁川敎育大學校 科學敎育硏究所 2011 과학교육논총 Vol.24 No.-