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Cd 處理에 對한 박달나무의 家系間 生理的 被害 및 抗酸化 反應 差異
吳昌泳,李景俊,李在千,韓心熙 한국임학회 2004 한국산림과학회지 Vol.93 No.7
본 연구는 Cd 처리에 대한 박달나무 유묘의 가계 간 엽록소 함량과 효소홀성에 의한 피해 및 항산화 내성 반응의 특성을 구명하고자 실시하였다. Cd 처리는 3가계의 박달나무 1년생 묘목에 0, 04, 08mM CdSO₄·8/3H₂O 용액을 이용하여 3수준으로 2개월간 실시하였다. 박달나무의 가계간 및 처리 간 피해내성반응은 엽록소 함량과 MDA(malondialdehyde) 함량, 단백질 함량, SOD(supetoxide dismutase) 활성을 이용하여 결정하였다. Cd 처리는 박달나무의 잎 내 광색소의 함량비를 변화시켰으며, 생리적 피해 지표인 MDA 함량은 모든 가계의 Cd 처리구에서 대소구보다 높게 나타났다. 또한 모든 Cd 처리구의 단백질 함량은 대조구보다 높았으며, MDA 함량과 단백질 한량은 Cd 처리 농도가 증가함에 따라 증가하였다. 한편 Cd 처리된 박달나무의 잎 내 SOD 활성은 대조구보다 증가하였으나, 0.8mM Cd 처리된 9번 가계 경우는 대조구보다 낮아졌다. 즉 박달나무는 Cd 처리에 의해 광색소 함량의 변화 및 MDA 함량 증가와 같은 생리적인 피해를 나타냄과 동시에 단백질 함량과 SOD 활성 증가를 통해 내성 반응을 나타냈다. 그러나 이리한 생리적인 피해 및 내성 반응은 가계 간에 뚜렷하게 달랐다. We investigated differences among half-sib families of Betula schmidtii seedlings in the effect of three Cd levels on physiological injury and antioxidative reaction. One-year-old seedlings of B. schmidtii were treated with 0, 0.4 and 0.8mM CCdSO₄·8/3H₂O for two months. Physiological injury and antioxidative reaction to three levels of Cd treatment determined with using photosynthetic pigments, MDA, protein contents, and SOD activity in the leaves of three half-sib families of B. schmidtii. The ratios of contents among photosynthetic pigments in the leaves of B. schmidtii were changed by Cd treatment. In additon, MDA content in the leaves of plants under Cd stress increased relative to control plants, and total protein content was also higher in the leaves of 0.4mM and 0.8mM Cd levels than control plants. The contents of MDA and total protein also increased with increasing the concentrations of Cd treatment. SOD activity in the leaves of three half-sib families increased by Cd treatment, but SOD activity of No. 9 family decreased relative to control plants at 0.8mM Cd level. In our trial, B. schmidtii treated with Cd represented physiological injuries such as the changes of photosynthetic pigments ratio and the increase of MDA content. At the same time, B. schmidtii showed tolerance responses against Cd toxicity through increasing the total protein content and SOD activity. But the physiological injury and tolerance responses were significantly different among three half-sib families exposed to 0.4mM and 0.8mM Cd.
Effect of Metformin on Cell Growth and Differentiation in Cultured Odontoblasts
오창영,김수관,고대산,유선경,김태훈,김춘성,박주철,김도경 대한구강생물학회 2017 International Journal of Oral Biology Vol.42 No.2
Metformin (1,1-dimethylbiguanide hydrochloride), derived from French lilac (Galega officinalis), is a first-line anti-diabetic drug prescribed for patients with type 2 diabetes. However, the role of metformin in odontoblastic cell differentiation is still unclear. This study therefore undertook to examine the effect of metformin on regulating odontoblast differentiation in MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells. As compared to controls, metformin significantly accelerated the mineralization, significantly increased and accelerated the expressions of ALP and Col I mRNAs, and significantly increased the accelerated expressions of DSPP and DMP-1 mRNAs, during differentiation of MDPC-23 cells. There was no alteration in cell proliferation of MDPC-23 cells, on exposure to metformin. These results suggest that the effect of metformin on MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells, facilitates the odontoblast differentiation and mineralization, without altering the cell proliferation.
오창영,한상억,이경준,김장수,오찬진,지동현 한국육종학회 2009 한국육종학회지 Vol.41 No.3
소나무 수형목 풍매차대를 대상으로 수고생장에 대하여 일반조합능력을 평가하고자 본 연구를 수행하였다. 본 연구에 사용된 가계는 총 232가계로 각 수령별 10년생 230가계, 20년생 184가계, 30년생 122가계를 대상으로, 차대검정림에서의 수고 생장 조사 자료를 이용하였다. 차대검정림은 1975년부터 조성하기 시작하여 1987년까지 모두 7회에 걸쳐 1~4 지역에 조성하였다. 이러한 조성 년도, 지역의 차이를 표준화를 통하여 균일한 수치로 보정하여 This study was conducted to select superior families based on the open-pollinated (OP) progeny tests of P. densiflora. A total of 232 OP families were analyzed for relative height growth. The OP progeny test trials were established at 1 to 4 sites from 1975 to 1987. To minimize temporal and spatial variation, we applied the standardization method for family selection. In each progeny test, superior and inferior families were selected at ages of 10, 20 and 30. Relative height growth rate (RHGR), growth speed at a given time unit, was comparatively high at age of 10 with range from 0.1 to 0.6 and showed a large variation among families. However, after age 15, the RHGR was low (average 0.04) and also the variation was not significantly different among families. To reduce selection errors due to age differences (from age 23 to 35) of tests, we made the family selection after age 15 when the values of RHGR were stable. Height growth at each age was transformed to be height growth at age 35 based on the RHGR. As the results, family CB2, CB3, KW99 and KW2 were selected as superior families and KW158, KW22, KB40 and GG1 were considered as inferior ones, respectively. Rank correlations (r) between test ages and selection age 35 were high and statistically significant; r = 0.881 between age 30 and 35, 0.653 between age 20 and 35, and -0.222 between age 10 and 35.
박달나무의 반형매 가계간 Cd Phytoremediation과 축적 특성
오창영,이재천,한심희,김판기 한국농림기상학회 2004 한국농림기상학회지 Vol.6 No.3
The main purpose of this study was to select a B. schmidtii population which has high cadmium tolerance and remediation and to determine the difference of cadmium uptake patterns among populations. One-year-old B. schmidtii seedlings were treated with 0, 0.4, 0.8mM CdSO₄ *3/8H₂O for two months. Cadmium concentrations in different positions of stem and cadmium concentrations and contents of leaves, stems and roots were analyzed. Also soil cadmium concentrations were analyzed. B. schmidtii was highest in root and lowest in shoot tip, showing a gradual decrease from root to shoot tip. The shoot to root Cd concentration ratios were over 1.26. It is concluded that B. schmidtii has good potential for phytoextraction as a shoot accumulator, which can be used for remediation of cadmium-contaminated areas. But tolerance differs between populations. Therefore B. schmidtii should be used as a means of phytoremediation after selection for Cd tolerance is performed.