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두유에서 Sacchasomyces uvarum 과 Lactobacillus acidophilus의 혼합배양
공인수,이정수,정용준,류인덕,오두환,유주현,Kong, In-Soo,Lee, Jung-Soo,Chung, Yong-Joon,Lew, In-Deok,Oh, Doo-Whan,Yu, Ju-Hyun 한국식품과학회 1987 한국식품과학회지 Vol.19 No.4
Sacchasomyces uvarum과 Lactobacillus acidophilus를 두유에서 단독 및 혼합배양하였을때 산생성에 미치는 조건은 검토하였다. 접종비율에 의한 산생성은 L. acidophilus와 S. uvarum의 접종비가 2 : 1일때 가장 높은 산도를 보였으며 배양시간은 16시간부터 높은 산도를 유지하였다. 배양온도는 $37^{\circ}C$가 가장 좋았으며 두유에 sucrose를 첨가하여 단독배양하면 산생성의 증가는 없었으나 혼합배양한 결과 산도가 증가하였다. Sucrose 2%와 skim milk 3%를 첨가하여 혼합배양하면 두유만을 사용하여 혼합배양한 것에 비하여 약4배의 산생성 증가를 보였다. S. uvarum의 두유 배양여액을 열처리하지 않고 두유에 첨가하여 L. acidophilus를 단독배양하면 단독배양만으로도 높은 산생성을 보여주었으나 열처리한 배양여액이 첨가된 두유에 L. acidophilus를 단독배양하면 산생성의 증가가 전혀 나타나지 않았다. Among the several lactic acid bacteria, Lactobacillus acidophilus showed the highest acid production when it was cultured mixed with Sacchasomyces uvarum in soymilk. The highest acid production was obtained in 16 hrs of cultivation when the inoculation ratio of L. acidophilus and S. uvarum was 2:1 and the temperature was $30{\sim}37^{\circ}C$. The acid production was greatly enhanced by the addition of 2.0% sucrose. However, skim milk was not stimulatory in mixed culture. During mixed culture in soymilk, acid production was affected by the enzymatic reaction of yeast.
Ganoderma lucidum IY 009조다당 분획들의 항암활성과 항보체활성간의 상호관계
이권행,이준우,한만덕,정훈,김영일,오두환,Lee, Kweon-Haeng,Lee, June-Woo,Han, Man-Deuk,Jeong, Hoon,Kim, Young-Il,Oh, Doo-Whan 한국미생물·생명공학회 1994 한국미생물·생명공학회지 Vol.22 No.1
Antitumor polysaccharides were known to activate complement system and to increase specific serum proteins in mouse, and researcher reported that antitumor activity of polyasccharides might be correlated with their biological properties such as activation of complement system and increase of specific protein $L_{A}$, $L_{B}$ and $L_{C}$ within the mouse serum. In case of several Ganoderma lucidum, there was no correlation between their antitumor activities and their bioloical properties, but the antitumor activities against sarcoma 180 of the alkali extracted crude polysaccharide fractions of the Ganoderma lucidum IY 009. AS, T, AI and M were correlated with their bioloical properties such as anticomplementary activity and intensity of mouse serum protein $L_{A}$, $L_{B}$ and $L_{C}$.
식품저장수명에 대한 ceramic film package의 효과
홍상필,오두환,양융,선보경 연세대학교 산업기술연구소 1993 논문집 Vol.25 No.1
The positive of effects of ceramic film package on the shelf life of foods were compared with those of P.E. film. compared with P.E. film package, ceramic film package inhibited growth of microorganisms and decreased lipid oxidation velocity. Ceramic film package extended the shelf life of Vit. C and stabilized chlorophyll pigment. Total score in the organoleptic tests for ceramic film packed foods was higher than that for P.E. film packed foods.
Ganoderma lucidum IY 009조다당 분획들의 항암활성과 항보체활성간의 상관관계
이권행,이준우,한만덕,정훈,김영일,오두환 한국산업미생물학회 1994 한국미생물·생명공학회지 Vol.22 No.1
항암활성 다당류들은 mouse의 보체계를 활성화시키고 serum내 특정 혈청 단백질을 증가시키는 것으로 알려져 왔다. 여러 연구자들의 의하면, 다당류의 항암활성은 보체계의 활성화와 mouse serum의 특정 단백질인 L_A, L_B 및 L_C등의 증가와 관련이 있다고 보고하였다. 6종의 Ganoderma lucidum의 균사체로부터 얻은 조다당의 항암활성과 항보체활성 사이에는 상관관계가 없었으나, G. lucidum IY 009의 균사체를 알칼리로 추출하여 얻은 조다당 분획- AS, T, AI 및 M 사이에서 sarcoma 180에 대한 항암활성이 높은 것이 항보체활성이 높게 나타났다. 한편 AS, AI 및 M을 투여한 mouse의 serum중의 L_A, L_B 및 L_C protein의 강도는 항암활성과 비례적으로 나타났으나, 열수추출 분획 H의 경우는 비례하지 않았다. Antitumor polysaccharides were known to activate complement system and to increase specific serum proteins in mouse, and some researcher reported that antitumor activity of polysaccharides might be correlated with their biological properties such as activation of complement system and increase of specific protein L_A, L_B and L_C within the mouse serum. In case of several Ganoderma lucidum, there was no correlation between their antitumor activities and their bioloical properties, but the antitumor activities against sarcoma 180 of the alkali extracted crude polysaccharide fractions of the Ganoderma lucidum IY 009. AS, T, AI and M were correlated with their bioloical properties such as anticomplementary activity and intensity of mouse serum protein L_A, L_B and L_C.