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군유전체학을 활용한 후코이단 반응 토양 미생물 분리 및 식물생장촉진 활성 규명
양소희 ( Sohee Yang ),박효선 ( Hyosun Park ),김의연 ( Euyeon Kim ),구연종 ( Yeonjong Ko ) 한국환경농학회 2020 한국환경농학회 학술대회집 Vol.2020 No.-
Seaweed and seaweed extract have been studied and developed as a crop fertilizer because they have various plant growth promoting compounds and antibacterial reagents. To use seaweed as fertilizer, the major carbohydrates of seaweed, fucoidan and alginate, should be digested efficiently in the soil. In this paper, we looked at changes in the bacterial community by adding fucoidan and alginate. With 16s ribosomal DNA analysis, bacteria that was either fucoidan and alginate resistant (increasing their number) or susceptible (decreasing their number) were selected successively from paddy soil. Most of the bacteria that increased their number with fucoidan and alginate had specificity to each carbohydrate, while the decreasing bacteria showed susceptibility to both carbohydrates. This report found some useful bacteria to apply as a biofertilizer with seaweed and also demonstrated that a high throughput approach is an efficient method to find bacteria with specific properties
CRISPR-Cas9 시스템을 활용한 제초제 저항성 토마토 개발 전략
김의연 ( Euyeon Kim ),박효선 ( Hyosun Park ),양소희 ( Sohee Yang ),구연종 ( Yeonjong Koo ) 한국환경농학회 2020 한국환경농학회 학술대회집 Vol.2020 No.-
The development of herbicide-tolerant plants provided a convenient farming method. The five most popular herbicides are divided into glyphosate,sulfonylureas/imidazolinones, glufosinate, norflurasone and oxyfluorfen depending on the reaction target by their mode of action and these herbicides inhibit 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), acetohydroxyacid synthase (AHAS), glutamine synthetase (GS), Phytoene desaturase (PDS) and protoporphyrinogen oxidase (PPO), respectively. Inhibition of EPSPS and AHAS reduces the biosynthesis of phenolic and branched amino acids, respectively, and inhibition of GS and PDS enhances the production of reactive oxygen species and induces plant necrosis. Inhibition of PPO decreases chlorophyll biosynthesis and inhibits plant photosynthesis. These herbicides induce plant death by interacting with their target proteins and the development of herbicide resistant plants is based on the discovery of mutant proteins insensitive to these herbicides. We reviewed the development of herbicide-tolerant plants and derived target amino acids for the production of herbicide-tolerant proteins using the CRISPR/Cas9 system in tomatoes.
김인중(Injung Kim),나기현(Kihyun Na),양소희(Sohee Yang),장재민(Jaemin Jang),김윤종(Yunjong Kim),신원영(Wonyoung Shin),김덕중(Deokjung Kim) Korean Institute of Information Scientists and Eng 2017 정보과학회논문지 Vol.44 No.8
T-commerce is technology-fusion service on which the user can purchase using data broadcasting technology based on bi-directional digital TVs. To achieve the best revenue under a limited environment in regard to the channel number and the variety of sales goods, organizing broadcast programs to maximize the expected sales considering the selling power of each product at each time slot. For this, this paper proposes a method to predict the sales of goods when it is assigned to each time slot. The proposed method predicts the sales of product at a time slot given the week-in-year and weather of the target day. Additionally, it combines a statistical predict model applying SVD (Singular Value Decomposition) to mitigate the sparsity problem caused by the bias in sales record. In experiments on the sales data of W-shopping, a T-commerce company, the proposed method showed NMAE (Normalized Mean Absolute Error) of 0.12 between the prediction and the actual sales, which confirms the effectiveness of the proposed method. The proposed method is practically applied to the T-commerce system of W-shopping and used for broadcasting organization.