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Fabrication of Sericin into Micro- and Macro Size Materials and its Application
양세준,Yijin Kang,Yejin Cho,신봉섭,이기훈 한국잠사학회 2022 International Journal of Industrial Entomology Vol.44 No.2
Over the past decades, silk sericin (SS) received increasing attention in the academic and industrial fields. In nature, SS acts as a glue that holds the two strands of silk fibrils together. However, recent works suggest that SS might have a more diverse role during the silk spinning process, such as stabilizing the SF in the silk gland. On the other hand, the sericulture industry has been trying to find novel applications for SS discarded from the silk fabric manufacturing process. Recovery and refining of SS would be the first step of the recycling of SS. Using a proper solvent SS could be shaped into various forms, such as spherical beads, microparticles, fibers, and films. Moreover, the applicability of these SS materials has been investigated in various fields such as cosmetics, templates for nanomaterials, drug delivery, heavy metal adsorption, and enzyme immobilization.
양세준,오병근 한국식물생명공학회 1998 식물생명공학회지 Vol.25 No.5
벼 약배양 배지에 첨가되는 배지응고제의 종류와 농도가 캘러스형성 및 식물체재분화에 미치는 영향을 구명하기 위하여 실험한 결과는 다음과 같다. 배지응고제인 Bacto agar(Difco, 04140-01), Agrose(Sigma, Type 1) 0.4% 처리에서 각각 39%, 55%의 캘러스형성율을 보였으나 농도가 높아질수록 캘러스 형성율이 현저하게 감소하였다. Gelrite(Kelco, 143364)의 0.2~0.8%처리에서는 농도에 따른 차이가 인정되지 않았으며 0.6% 처리에서 44%의 캘러스 형성율을 보였다. 고농도 배지응고제 처리에서 형성된 캘러스는 생장이 늦은 반면 작고 단단하며 백색에 가까운 배발생적인 특성을 보였다. 식물체재분화율은 고농도 배지응고제 처리에서 형성된 캘러스에서 양호하였으며, 또한 고농도 배지응고제가 첨가된 식물체분화배지에서 높았다. 저농도 배지응고제 처리에서 형성된 약치상후 60일된 캘러스는 식물체 재분화율이 급격히 감소하였다. 치상된 약에 대한 최종적인 식물체분화 효율을 향상시키기 위해서는 0.6∼0.8% Gelrite 첨가배지에서 형성된 캘러스를 1.6% Bacto agar 및 Agarose 또는 0.8% Gelrite 식물체분화배지에 이식할 것을 추천한다. To detect the effects of gelling agent brands and concentration on rice anther culture, anthers of rice(O. sativa L. japonica, cv, Nagdongbyeo) were inoculated on N6-Y1 basic media supplemented with 0.4~1.6% Bacto agar(Difco, 04140-01), Agarose(Sigma, Type 1) and 0.2~0.8% Gelrite(Kelco, 143364) as gelling agents. On 0.4% Bacto agar and Agarose media, the frequency of callus formation which was significantly decreased in proportion to gelling agent's concentration was 39% and 55%, respectively. On 0.6% Gelrite media, the frequency of callus formation which was not statistically significant among the 0.2~0.8% concentration was 44%. Calli derived from the higher concentration of gelling agents showed embryogenic with slow growth, small, whitish and hard shape compare to that of the lower concentration. The frequency of green plant regeneration was high not only in calli derived from the higher concentration but also in plant regeneration medium with the higher concentration after callus transfer. Calli derived from the higher concentration was effective to maintain the frequency of green plant regeneration up to 60 days after anther inoculation. Introduction of 0.6~0.8% Geltite for callus formation, then transferred 1.6% Bacto agar and Agarose or 0.8% Gelrite for green plant regeneration was effective to increase anther culture efficiency.
除草劑 Saturn-S에 對한 水稻品種間 反應 및 抵抗性遺傳
楊世準,김낭춘 慶北大學校 새마을硏究所 1980 農村과 科學 Vol.3 No.-
除草劑 Saturn-S에 對한 水稻品種間의 藥害反應 및 抵抗性遺傳樣式을 空明하여 除草劑 藥害抵抗性品種育成의 基礎資料를 얻고자 本硏究를 遂行하였으며 얻어진 結果는 다음과 같다. 室內試驗에서 水稻品種間 藥害反應은 苗齡, 溫度, 土性, 土深, 畓田條件에 關係없이 大休로 같은 傾向을 나타내었다. 室內試驗에서 Japonica型은 全品種이, Indica x Japonica型은 水原264號, 密陽21號, 密陽42號가 抵抗性이었으나 其他 많은 品種들은 이들보다 藥害抵抗性이 弱하였고, 特히 統一, 維新, 水原284號, 裡里338號 및 魯豊등은 感受性을 나타내었다. 圃場試驗에서도 Japonica型은 全品種이, Indica x Japonica型은 17品種中 密陽21號, 水原264號, 密陽42號가 그리고 Indica型은 64品種中 Kn1b-361-1-8-6-9-1만이 抵抗性을 나타내어 室內試驗과 같은 樣相을 나타내었다. F_1 및 F_2에 있어서 藥害抵抗性은 優性으로 作用하는 것으로 推定되었다. This study was conducted to obtain basic information on the rice breeding by clarifying rice varietal responses and inheritance of tolerance to herbicide(Saturn-S). Rice varietal responses to Staturn-S showed the same tendency regardless of various treatment conditions such as application time, temperature, soil texture, soil depth, land condition in pot experiment. In pot experiment all Japonica type varieties and Suwon #264, Milyang #42, Milyang #21 among Indica x Japonica type varieties were tolerant but the other Indica x Japonica type varieties such as Tongil, Yushin, Suwon#284, Iri#338, Noupoung were susceptible to Saturn-S. In field experiment all Japonica type varieties and Milang#21, Milyang#42, Suweon#264 among 17 Indica x Japonica type varieties were tolerant but Knlb-361-1-8-6-9-1 among 64 Indica type varieties was tolerant to Staturn-S. Tolerance seemed to be dominance over susceptibility by the reaction distribution pf parents, F_1, and F_2 plants.
양세준,김연규,최임수,조영찬,황흥구,홍하철,김명기,오명규,신영섭,이점호,최용환 한국육종학회 2009 한국육종학회지 Vol.41 No.4
Cheongan is a new japonica rice variety developed from a cross between SR15225-B-22-1-2-1 and Iksan431 in summer season, 1997 by National Institute of Crop Science, RDA. The line SR15225-B-22-1-2-1 has good canopy architecture and multi-disease and insect resistance, and Iksan431 has translucent milled rice and good eating-quality. Heading date of Cheongan is August 13 in central lowland and mid-mountainous areas. "Cheongan" having culm length of 84 cm shows relatively semi-erect pubescent leaf blade and rigid culm, tolerance to lodging with and good canopy architecture. This variety has 14 tillers per hill and 126 spikelets per panicle. It shows tolerance to heading delay and spikelet sterility comparable to Hwaseongbyeo when exposed to cold stress. Leaf senescence of Cheongan progresses slowly during the ripening stage and the viviparous germination ratio was 59 %, similar to that of Hwaseongbyo. "Cheongan" shows moderately resistance to blast disease, but susceptible to stripe virus and brown planthopper. The milled rice of "Cheongan" exhibits translucent, clear non-glutinous endosperm and medium short grain. It shows similar amylose content of 18.7%, gelatinization temperature, and similar palatability of cooked rice compared to Hwaseongbyeo. The milled rice yield of this cultivar is about 5.54 MT/ha at ordinary season culture in local adaptability test for three years. Especially, "Cheongan" has better milling properties of higher 98.4% and 73.9% in the percentage of head rice in milled rice and milling recovery of head rice, respectively, than those of Hwaseongbyeo. "Cheongan" could be adaptable to the central and mid-southern plain area, and mid-western coastal area of Korea.