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宋完燁,金炯武,盧台煥,李斗求 전북대학교 농업과학기술연구소 1996 農大論文集 Vol.27 No.1
Twenty phenolic compounds originated from plants were tested in nutrient-broth bioassay for screening inhibitory activity to rice seedborne Pseudomonas avenae, P.glumae and Xanthomonas oryzae pv. oryzae. Flavone and esculetin strongly inhibited X. o. pv. oryzae only. Myricetin inhibited .P. glumae and X. o. pv. oryzae but showed strong minimal inhibition concentration and minimal bactericidal concentration under a concentration of 25μg/ml. Cinnamic acid ethylester, naringenin and phloretin showed significant inhibitory activity towards tested all strains of P. avenae, P. glumae and X. o. pv. oryzae. Cinnamic acid ethylester was moderately inhibitory (100-200 μg/ml) and naringenin (50- 200 μg/ml) and phloretin (25-50 μg/ml) were strongly inhibitory. At a concentration of 100 μg/ml, phloretin were found to be bactericidal to all the tested strains of three species.
Streptococcus mutans의 우식 활성에 미치는 Propolis의 효과
송완엽,백병주,주훈,김대범,송요한 大韓小兒齒科學會 1995 大韓小兒齒科學會誌 Vol.22 No.1
Propolis, a natural resinous product collected by honey bees, is known to exhibit various biological activities including anti-microbial, anti-inflammatory, and anti-tumor activities. The present study was designed to investigate the effects of ethanol extracted propolis(EEP) on the growth, glucosyltransferase(GTase) activity, and adherence to the saliva-coated hydroxyapatite(S-HA) beads of Streptococcus mutans JC-2(serotype c), S.mutans LMM-7(serotype e), and S. mutans OMZ-175(serotype f) in vitro. We found that the EEP had minimal inhibitory concentrations of 500-1000㎍/㎖ against S.mutans strains when these strains were cultured for 48 hours, and inhibited both water-insoluble and water-soluble glucan synthesizing GTase activities of S. mutans strains to the 93-100% at the concentration of 1600㎍/㎖, and also we found that the EEP inhibited the adherence of S. mutans strains to S-HA beads to the 76-96% at the concentrations of 200㎍/㎖. These results indicate that EEP has anti-cariogenic activity in vitro.
벼 종자에서 Acidovorax avenae subsp.avenae 검정을 위한 선택배지
강미형,송완엽,김형무 전북대학교 농업과학기술연구소 2000 農大論文集 Vol.31 No.1
A semi-selective agar medium (YTA) was developed for the detection of a bacterial brown stripe pathogen, Acidovorax avenae subsp. avenge (Aaa), from rice seeds. The new medium YTA contained yeast extract 9.0 g, tween 80 10.0 mℓ, CaCl2 · H2O 0.6 g, FeSO4 · 7H20 100.0 mg, victoria blue B 40.0 mg, bromthymol blue 15.0 mg, 5-flurouracil 62.5 mg and agar 15.0 g per litter. Colonies of Aaa were 2 - 3 mm in diameter, smooth, round, convex and blue after 4-5 days after incubation at 28℃ ,Aaa strains formed blue colonies with lipid-hydrolysis zone on YTA media containing tween 80, Victoria blue B and bromthymol blue. The blue color with lipid hydrolysis zone was characteristic for the bacterial brown stripe pathogen and used to differentiate colonies of Aaa from those of other bacteria on YTA medium. Addition of 5-flurouracil reduced other bacterial populations on YTA medium. The mean recovery of 8 strains of Aaa on the YTA medium was 96.5 percent in comparison to YDC medium. The other bacteria from rice seed lots on YTA medium was reduced to 70.8 percent of that on YDC medium. We concluded that this agar plating using YTA medium is a simple method of assaying large numbers of rice seed extracts for f. avenae subsp. Avenae
고추냉이 세균성 근경 부패병의 발생 및 병원균의 분리 동정
김형무,송완엽,강미형,소인영 전북대학교 농업과학기술연구소 1996 農大論文集 Vol.27 No.1
A disease occurrence of rhizome rot at the Japanese horse radish growing fields in Chonbuk area was investigated and soft rot-causing bacteria were isolated and identified by morphological and biochemical characteristics to verify a causal bacterium of rhizome soft-rot disease of Japanese horse radish. The rhizome soft-rot was occurred on April and continued to October·. It was most severe on August but decreased on September. Disease occurrence reflected regional difference such as 38% in Chonju and Iksan and 27% in Muju. Four major bacterial genera, Erwinia, Pseudomonas, Xanthomonas and Corynebacterium, were isolated and showed the isolation frequency of 54.6%, 17.2%, 21.2% and 6.8%, respectively. They also showed a seasonal fluctuation of isolation frequencies in genera and Erwinia sp. was the most dominant species during the high temperature season. Rhizome soft rot-causing isolates were selected by the pathogenicity test. They showed negative results in Gram's staining, growth in 5% NaCl and growth on KCN media but positive in casein hydrolysis and gelatin liquefaction. They utilized galactose, lactose, trehalose and citrate but did not utilize maltose, melibiose, raffinose and inullin. They also showed the soft-rot symptom on the petiole and rhizome of the horse radish but weak soft rot on potato tuber. This causal pathogen was identified as Erwinia carotovora subsp. wasabiae by these results of bacteriological characteristics.