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재조합 Interleukin 1α가 각질형성세포의 증식 및 Superoxide Radical과 Superoxide Dismutase 활성도의 조절에 미치는 영향
서성준,홍창권,노병인 중앙대학교 의과대학 의과학연구소 1993 中央醫大誌 Vol.18 No.1
There are some evidences that an interleukin(IL)1a enhances murine as well as human keratinocyte proliferation in vitro. However, according to its results of experiments using IL-1a by several investigators, it is also conceivable that IL-1a did not stimulate keratinocyte proliferation. To determine whether keratinocyte proliferation by IL-1a occurred, the present study was undertaken. In addition, we measured superoxide radical and superoxide dismutase to identify the intracellular biochemical events associated with effects caused by IL-1a, Primary keratinocyte cultures from neonatal foreskins were grown in complete MCDB 153 medium. Measurements of superoxide radical and superoxide dismutase(SOD)activity were performed according to Babior and Fridovich methods, respectively. The rusults follows: 1. Cell numbers of keratinocytes without treatment of recombinant IL-1a(rIL-1a) were 6.67+-0.91*10^4, 8.23+-0.65*10^4, 1.09+-0.12*10^5 at 4, 6 and 8 days, respectively. Cell numbers of keratinocytes with treatment of 10ng/㎖ rIL-1a were 6.20+-0.92*10^4, 8.73+-1.16*10^4, 1.58+-0.10*10^5 at 4, 6, and 8 days,respectively. Compared with that of untreated case, approximately 1.5fold increase in cell numbers was observed after 8 days culture with treatment of 10ng/㎖ rIL-1a. 2. Release of superoxide radical were measured after 30 minutes exposure to 0.1, 1.0, 10ng/㎖ rIL-1a, the results show 1.61+-0.065nmol/1*10^5, 2.63+-0.59nmol/1*10^5, 3.68+-0.61nmol/1-10^5 respectively, Significant superoxide radical release was detectable at Ing/㎖ and 10ng/㎖ rIL-1a compared with 0.68+-0.19nmol/1*10^5 of untreated case(p<0.05). 3. Total SOD activity without treatment of rIL-1a at 12 hours was 20.50+-8.61, Total SOD activity after 12 hours treatment 0.1, 1.0, 10ng/㎖ rIL-1a were 99.32+-14.84, 115.25+-14.32, 130.85+-10.80 respectively, which suggested that rIL-1a induced increase of total SOD significantly(P<0.05), rIL-1a stimulate total SOD induction in a dose and time dependent manner, although the effects was not outstanding. Mn SOD activity after treatment of 10ng/㎖ rIL-1a at 24 hours was 104.34+-11.61, which was approximately 11 fold of that of untreated case. rIL-1a also induced the Cu, Zn SOD activity, but the effect was not so much as in the case of Mn SOD. In summary, the results indicate that rIL-1a enhances human keratinocyte proliferation and superoxide radical released by rIL-1a may be play a role in human keratinocyte proliferation. Also production of total SOD, especially Mn SOD in response to rIL-1a induced and scavenged overproduced superoxide radical which in turn may result in protection of keratinocytes.