토끼의 Aflatoxin B₁중독증에 대한 인삼엑기스의 효과 : 임상, 생리적 및 병리학적 변화 Clinical, Physiological and Pathological Changes

김차용,변유성,문평일,정덕화,김종수 慶尙大學校 1987 論文集 Vol.26 No.1

Chinchilla 계통 1~1.5㎏ 되는 토끼 30마리를 5군 으로 나누어 체중㎏당 제 1군은 대조군으로서 Cornoil 0.3ml 씩 제 2군은 aflatoxin B? 0.03mg과 인삼 엑기스 8mg을 제 5군은 aflatoxin B? 0.06mg과 인삼엑기스 8mg을 3주동안 카테타로 경구 투여하여 임상, 혈액학적 및 병리학적 변화를 관찰하였다. 1.사료소비량 감소, 침울, 쇠약, 혼수, 체중증가율감소등과 같은 임상증상은 대조군을 제외한 전 처리군에서 독을 투여후 3일째 부터 전처리기간을 통하여 나타났으나 aflatoxin B? 단독처리군에서 인삼엑기스 혼합처리군보다 더 현저하게 나타났다. 2. 혈청 효소와 AST, ALT는 독물투여후 7일부터 인삼엑기스 혼합투여군 보다 aflatoxin B? 단독 투여군에서 높게 증가하였다. 3.전처리군에서 적혈수용적, 헤모글로빈농도는 영향을 받지 않았고, 혈장단백질은ㅇ 독물투여후 14일이후 aflatoxin B?단독 처리군에서 현저하게 감소하였다. 4. Aflatoxin B?과 인삼엑기스 혼합 투여군 보다 aflatoxin B? 단독투여군에서 간장의 공포변성,담관증생, 핵농축, 붕괴, hepatic fibrosis와 임파절 및 비장에서 현저한 임파양 세포의 소실을 나타내었다. The present study has been carried out to investigate the effectsof ginseng extract on induced chronic aflatoxicosis in rabits. Groups of rabbits were treated orally with aflatoxin B? and mixture of ginseng extract for 21 days with a dosage of 0.03mg, 0.06mg of the toxin and 0.03mg or o.o6mg of the toxin mixture of 8mg of ginseng extract or 0.3ml corn oil s place to per kg body weight. The results obtained were is follows. 1. Sings o toxicosis included reduction of feed consumption emaciation, decrese of body weight and coma revealed in both groups treated with 0.03mg pr 0.06,mg of aflatoxin B?/Kg/B.W than groups 4.5 after days 3. 2. In groups 2, 3(aflatoxin B? 0.03, 0.06mg), Activity of asparate aminotransferase(AST) and alanine aminotransferase (ALT)were increased sigificantly in dats7, 14 and 21. 3. In all groups, Mean values for packed cell volume(PCV)and hemogobin were unremarkasble, but plasma total protein were decreased in groups 2,3than 4,5 groups on days 14 and 21. 4. The rabits treated with aflatoxin B?(2,3 groups)showed markedly degenerative chasnges ranging from swelling and decreaseed granularity to marked vacuolation, hepatic necrosis, fibrosis and bile duct, Proliferation and depletion of lymphocytes in lymphocytes in lymphonde, spleen than groups 4,5.

학술발표(學術發表) 연제(演題) 및 초록(抄錄) 제일부(第一部): 공중위생학(公衆衛生學) 분야(分野) : 1. 닭고기에서 병원성 및 변질미생물의 감소를 위한 염소 및 유산처리 효과

이철현,변유성,황보원,강호조 한국가축위생학회 1998 학술대회논문집 Vol.1998 No.-

돼지 분만 시기에 조절에 관하여 III. 자궁 평활근의 운동성에 대한 Histamine의 영향

박상은,황보원,변유성,조광제 한국동물위생학회 1995 韓國家畜衛生學會誌 Vol.18 No.2

The effects of histamine were investigated on the uterine smooth muscle motility in the pig. The results were summarized as fellows : 1. Histamine caused the contraction of the porcine uterine smooth muscle and the contractile responses increased between the concetration of histamine $10^{-8}$ and $10^{-5}$ M with a dose-dependent manner. 2. The contractile response Induced by histamine ($10^{-6}$ M) was completely blocked by pretrevatment with $H_1$-histaminergic receptor blocker, pyrilamine($10^{-6}$ M) 3. The contractile response induced by histamine($10^{-6}$ M) was increased by pretreatment with $H_2$-histaminergic receptor blocker, cimetidine($10^{-6}$ M) From these results, it was concluded that the effects of uterine smooth muscle by histamine were the contraction mediated by $H_1$-histaminergic receptor and the relaxation mediated by $H_2$-histaminergic receptor in pig.

돼지 분만 시기의 조절에 관하여 I. 자궁 평활근의 운동성에 대한 부교감 신경과 Cholinergic Agonist의 영향

박상은,황보원,변유성,조광제 한국동물위생학회 1994 韓國家畜衛生學會誌 Vol.17 No.3

To elucidate the action of the cholinergic nerve on the isolated uterine smooth muscle of the pig, effects of electrical transmural nerve stimulation and acetylcholine were investigated on the pretreatment of the physostigmine ; cholinestrase inhibitor and atropine ; cholinergic receptor blocker from physiograph. 1. The contractile response induced by acetylcholine was responsed in the concentration of >10^{-8}$ M at first and the maximum contractility was concentration of $10^{-6}$ M. 2. The contractile response induced by electrical transmural nerve stimulation(20 V, 0.5 Msec, 20 sec) was the frequency(2-64 Hz) -dependent manner. 3. The contractile response induced by acetylcholine was completely blocked by the pretreatment with cholinergic receptor blocker, atropine and was increased by the pretrement of cholinestrase inhibitor, physostigmine. 4. The contractile response induced by electrical transmural nerve stimulation was completely blocked by the pretreatment with cholinergic receptor blocker, atropine, and was increased by the pretretment of cholinestrase inhibitor, physostigmine. These findings suggest that it was powerful excitatory action by cholinergic nerve on uterine smooth muscle of the pig.

돼지 분만 시기의 조절에 관하여;IV. 자궁 평활근의 운동성에 대한 APT의 영향

박상은,황보원,변유성,조광제 한국동물위생학회 1996 韓國家畜衛生學會誌 Vol.19 No.2

The effcets of adenosine 5'-triphosphate(ATP) were investigated on the uterine smooth muscle motility in the pig. The results were summarized as follows: 1. The effects of the porcine uterine smooth muscle and the contractile responses increased between the concentration of ATP $10^{-5}$ and $10^{-3}$ M with a dose-dependent manner. 2. The contractile response induced by ATP($10^{-4}$ M) was not blocked by pretreatment with cholinergic receptor blocker, atropine ($10^{-6}$ M) 3. The contractile response induced by ATP ($10^{-4}$ M) was not blocked by pretreatment with $\alpha$ -adrenergic receptor blocker, phentolamine(10$^{-6}$ M) and ${\beta}$-adrenergic blocker, propranolol ($10^{-6}$ M). 4. The contractile response induced by ATP($10^{-4}$ M) was not appeared in 4Ca^{++}$ -free medium. As the concentration of $Ca^{++}$ in $Ca^{++}$ -free medium was increased, the contractile response induced by ATP ($10^{-4}$ M) was enhenced but was completely inhibited by pretreatment with $Ca^{++}$ -channel blocker, papaverine($10^{-6}$ M) or verapamil($10^{-6}$ M). From these results, it was conclued that the effects of ATP were the contraction mediated by purinergic receptor in uterine smooth muscle of pig.

닭고기에서 병원성 및 변질미생물의 감소를 위한 염소와 유산의 병용처리 효과

강호조,이철현,변유성,황보원 경상대학교 축산진흥연구소 1999 畜産振興硏究所報 Vol.26 No.1

도계처리 단계별 도체와 처리수의 세균오염 및 염소처리 효과

이철현 ( Chul Hyun Yi ),변유성 ( You Sung Byun ),황보원 ( Bo Won Hwang ),조광제 ( Kwang Je Cho ),강호조 ( Ho Jo Kang ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.2

This study was carried out assess the effect of the chlorine treatment into water for processing chicken products in each stage of slaughtering, with a special viewpoint related reducing the viable number of microorganisms by which the water and the chicken body were contaminated. The mean bacterial number on chicken samples after picking process was log5.37±0.20~5.84±0.16CFU/㎠. When assessed by standard plate count method, it was the higher one than any other processing stage in which eviscerating, pinning, packaging, and chilling was followed in order of the mean bacterial number. The coliform bacterial numbers on carcasses after sampling from different processing stages were log2.11±0.63~2.88±0.25MPN㎠, which show almost similar numbers in each processing stage. But, after chilling process the number was decreased slightly. The bacterial counts in the water for scalding and chilling showed log3.43±0.59~5.06±0.21 and log 4.30±0.21~6.62±0.33CFU/ml, respectively. In the coliform counts for the water taken out from the 2nd chilling tank, the number was log1.97±0.35~2.91±0.22MPN/ml which showed higher than those of the 1st and the 3rd chilling tank water. The effect of chlorination in reducing the bacterial numbers was accepted at the residual chlorine concentration of 1mg/l by showing the reduction from 108 to 104CFU level and the numbers were decreased less than 10CFU at the concentration of 5mg/l, when assessed by viable cell counts. In conclusion, these results suggested that chlorination in chilling water with final concentration of 5mg/l was strongly recommended to reduce the bacterial numbers on final chicken products.

석탄 DB연계 시스템을 적용한 화력발전소 시뮬레이터 개발

강해수(Hae-Su Kang),유성연(Seong-Yeon Yoo),변승현(Seung-Hyun Byun),신만수(Man-Su Shin) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.12

Fossil power plant simulator has developed by using the design and operation data in the real power plant system. There are functions defined external parameters as atmosphere temperature, humidity, seawater temperature, grid voltage, grid frequency, coal information and so on in the simulator. Although various coals can be used during coal firing in the plant operation, only one design coal information applies in the simulator. In order that the simulator emulates and operates same procedure in the real power plant system, coal DB interface system is set up with fossil power plant simulator. In this paper, fossil power plant simulator with the coal database interfacing system is developed.

닭고기에서 병원성 및 변질미생물의 감소를 위한 염소와 유산의 병용처리 효과

이철현 ( Chul Hyun Yi ),변유성 ( You Sung Byun ),황보원 ( Bo Won Hwang ),강호조 ( Ho Jo Kang ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.22 No.4

In this studies, the ability of chlorine and lactic acid to reduce bacterial population of the pathogenic microorganisms were examined on artificially inoculated chicken skin. About 10(5) cells of staphylococcus aureus, salmonella enteritidis, listeria monocytogenes and escherichia coli O157:H7 were inoculated in chicken skin. The contaminated samples were washed for 1 min with sodium hypochlorite solutions that contained 2, 5, 10, 20 and 50mg/ℓ available chlorine and counted number of the agents. Viable population were no significantly difference (p ≥ 0.05) between concentration of chlorine and strains of the pathogens. In the samples inoculated with pathogens were washed in 20mg/ℓ chlorine and then stored at 5°C for up to 10 days, the initial counts of psychrotrophs and aerobic plate counts were 4.02 to 4.36 log cfu/cm2 and increased slightly in course of time. But 10 days after, the pathogens were a little reduced from 3.66~4.91 log cfu/cm2 to 2.54~4.66 log cfu/cm2. In the case of washed skin with solution of 20mg/ℓ chorine and 0.5% lactic acid then store at 5°C for up to 10 days, population of psychrotrophs and aerobic plate counts on chicken skin were markedly reduced immediately after treatment, but the numbers of contaminants were slightly increased after 6 and 8 days. Specifically, numbers of St aureus, S enteritidis, L monocytogenes and E coli 0157:H7 were reduced to 0.5, 0.4, 0.3 and 1.15 log cfu/cm2 after 10 days of storage, respectively, on aerobic plate counts.

톱밥 발효돈사(醱酵豚舍) 사육돈(飼育豚)의 내부기생충(內部寄生蟲) 조사(調査)

이병훈 ( Byung Hoon Lee ),황보원 ( Bo Won Hwang ),변유성 ( Yu Seong Byun ),이순선 ( Soon Sun Lee ),김차용 ( Cha Yong Kim ) 한국가축위생학회 1993 韓國家畜衛生學會誌 Vol.16 No.2

This study was performed to survey on the prevalence of swine internal parasites at 12 piggeries being operated by the sawdust fermentative pigsty in Kyongnam central area. To investigate the effects of viability of eggs, larva and cysts, temperatures at the underlayer of the sawdust floor and rooms of pens were taken four times each season for one year. In a pig farms which have been operated by the sawdust fermentative pigsty, eggs of Ascar is suum(8.8%), Trichur is suis(18.3%), Oesophagostomum sp(9.4%), Strongyloides ransomi(1.3%), Hyostrongylus sp(3.8%) and Metastrongylus apri(2.3%) were detected from 480 heads of pigs. Cysts and oocysts of Coccidia(12.7%), Balantidium coli(5.4%) were also detected form 480 heads of pigs. The change of the temperature in the pig room was variable between 13℃ to 27℃ according to outside condition, whereas that in underfloor was consistently stable between 28℃ to 31℃ which was strictly suitable for the development of eggs, larva and cysts.