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식용식물자원으로부터 활성물질의 탐색-XX. 순무(Brassica campestris ssp rapa)뿌리로부터 지질화합물의 분리
방면호,이대영,한민우,오영준,정해곤,정태숙,최명숙,이경태,백남인,Bang, Myun-Ho,Lee, Dae-Young,Han, Min-Woo,Oh, Young-Jun,Chung, Hae-Gon,Jeong, Tae-Sook,Choi, Myung-Sook,Lee, Kyung-Tae,Baek, Nam-In 한국응용생명화학회 2007 Applied Biological Chemistry (Appl Biol Chem) Vol.50 No.3
순무 뿌리로부터 활성 물질을 분리 동정 하기 위하여 80% MeOH 수용액으로 추출하고 이를 여과, 감압 농축하여 MeOH 추출물을 얻었다. 이를 EtOAc분획, n-BuOH분획, $H_{2}O$분획으로 나누었으며, EtOAc분획과 n-BuOH분획에 대해 silica gel 및 ODS column chromatography를 실시하여 5종의 화합물을 분리 정제하였다. $^{1}H-NMR$, $^{13}C-NMR$, DEPT spectrum 및 Mass spectrum 등을 통하여 palmitic acid methyl ester(1), linolenic acid methyl ester(2), linoleic acid methyl ester(3), ${\beta}-sitosterol$(4), daucosterol(5)으로 구조를 결정하였다. The roots of Brassica campestris ssp rapa were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_{2}O$. From the EtOAc and n-BuOH fractions, five compounds were isolated through the repeated silica gel column chromatographies. From the result of spectroscopic data including NMR and MS, the chemical structures of the compounds were determined as palmitic acid methyl ester (1), linolenic acid methyl ester (2), linoleic acid methyl ester (3), ${\beta}-sitosterol$ (4) and daucosterol (5).
식용식물자원으로부터 활성물질의 탐색 XXII.순무(Brassica campestris ssp rapa) 뿌리로부터 인돌 화합물의분리 및 hACAT 저해 활성
방면호,이대영,오영준,한민우,양혜정,정해곤,정태숙,이경태,최명숙,백남인 한국응용생명화학회 2008 Journal of Applied Biological Chemistry (J. Appl. Vol.51 No.1
The roots of Brassica campestris ssp rapa were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and H2O. From the EtOAc fraction, three compounds were isolated through the repeated silica gel and octadecyl silica gel (ODS) column chromatography. From the results of spectroscopic data including NMR and MS, the chemical structures of the compounds were determined as caulilexin C (1), indoleacetonitrile (2) and arvelexin (3). The arvelexin (3) has been isolated from this plant for the first time. Compounds 1, 2 and 3 showed inhibitory activity on human Acyl CoA: cholesterol transferase 1 (hACAT1) by 54.6±6.0%, 69.2±4.7% and 68.6±3.7%, and on human Acyl CoA: cholesterol transferase 2 (hACAT2) by 4.8±13.4%, 45.6±4.8% and 39.5±4.3%, respectively, at 100 μg/ml. The roots of Brassica campestris ssp rapa were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and H2O. From the EtOAc fraction, three compounds were isolated through the repeated silica gel and octadecyl silica gel (ODS) column chromatography. From the results of spectroscopic data including NMR and MS, the chemical structures of the compounds were determined as caulilexin C (1), indoleacetonitrile (2) and arvelexin (3). The arvelexin (3) has been isolated from this plant for the first time. Compounds 1, 2 and 3 showed inhibitory activity on human Acyl CoA: cholesterol transferase 1 (hACAT1) by 54.6±6.0%, 69.2±4.7% and 68.6±3.7%, and on human Acyl CoA: cholesterol transferase 2 (hACAT2) by 4.8±13.4%, 45.6±4.8% and 39.5±4.3%, respectively, at 100 μg/ml.
방면호,장태오,송명종,김동현,권병목,김영국,이현선,정인식,김대근,김성훈,박미현,백남인,Bang, Myun-Ho,Jang, Tae-O,Song, Myoung-Chong,Kim, Dong-Hyun,Kwon, Byoung-Mog,Kim, Young-Kuk,Lee, Hyun-Sun,Chung, In-Sik,Kim, Dae-Keun,Kim, Sung-Hoon,Par 한국응용생명화학회 2004 Applied Biological Chemistry (Appl Biol Chem) Vol.47 No.2
식용식물자원으로부터 활성소재를 찾기 위하여 흰씀바귀 뿌리를 80% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH및 $H_2O$로 용매 분획하였다. EtOAc와 n-BuOH 분획에 대하여 column chromatography를 반복하여 4종의 화합물을 분리하였다. 각각에 대하여 2D-NMR을 포함한 스펙트럼 데이터의 해석과 문헌 자료를 조사하여 zaluzanin C (1), $9{\alpha}-hydroxyguaian-4(l5),10(14),11(13)-triene-6,12-olide$ (2), $3{\beta}-O-{\beta}-D-glucopyranosyl-8{\beta}-hydroxyguaian-4(15),\;10(14 )-diene-6,12-olide$ (3), $3-O-{\beta}- D-glucopyranosyl-8{\beta}hydroxyguaian-10(14)-ene-6,12-olide$ (4)로 구조를 결정하였다. 이들 화합물에 대하여 ACAT(Acyl-CoA: cholesterol acyltransferase), DGAT (diacylglycerol acyltransferase) 및 FPTase(farnesyl-protein transferase)의 활성에 미치는 억제효과를 측정하였다. Compound 1과 Compound 2는 DGAT에 대한 활성억제효과에 있어서 $IC_{50}$ 값이 각각 0.13 mM, 0.10 mM로 나타났고, FPTase에 대하여는 각각 0.15 mM, 0.18 mM로 나타났으며, ACAT에 대하여는 약한 억제 활성을 나타냈다. 따라서 흰씀바귀는 항암 및 항고혈압의 소재 개발에 있어서 유용한 자원으로 활용될 수 있을 것이다. The root of lxeris dentata forma albiflora was extracted with 80% aqueous MeOH and solvent fractionated with EtOAc, n-BuOH and water, successively. From the EtOAc and n-BuOH fractions, four sesquiterpene compounds were isolated through the repeated silica gel and ODS column chromatographies. The chemical structures were determined as zaluzanin C (1), $9{\alpha}-hydroxyguaian-4(l5),10(14),11(13)-triene-6,12-olide$ (2), $3{\beta}-O-{\beta}-D-glucopyranosyl-8{\alpha}-hydroxyguaian-4(15),10(14 )-diene-6,12-olide$ (3), and $3{\beta}-O-{\beta}- D-glucopyranosyl-8{\beta}hydroxyguaian-10(14)-ene-6,12-olide$ (4) through the interpretation of several spectral data including 2D-NMR. Some showed the inhibitory effects on DGAT (Diacylglycerol acyltransferase), ($IC_{50}$ values of 1, 2: 0.13, 0.10 mM), the catalyzing enzymes of the intracellular esterification of diacylglycerol and FPTase (Famesyl-protein transferase), ($IC_{50}$ values of 1, 2: 0.15, 0.18 mM), the farnesylation enzyme for Ras protein charge of cancer promotion.
Anti-osteoporotic Activities of Fucosterol from Sea Mustard (Undaria pinnatifida)
방면호,이대영,Nam-In Baek,Hong-Hee Kim,Min-Woo Han,Yoon-Su Baek,정대균 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.2
Samples of the edible seaweed, sea mustard (Undaria pinnatifida) were extracted with 80% aqueous methanol, and the concentrated extract was partitioned with ethyl acetate, n-butanol, and water, successively. Isolated fucosterol (1) from the ethyl acetate layers using repeated silica gel and octadecyl silica gel (C_18, ODS-A)column chromatography and identified it using spectroscopic data including NMR, MS, and infrared spectroscopy (IR). Osteoclasts are specialized multinucleated cells with bone resorbing activity. The induction of c-Fos and NFATc1transcription factors by receptor activator of nuclear factor κB ligand (RANKL) is a key event in osteoclast differentiation. Isolated and identification compound 1 was investigated in vitro for their anti-osteoclastic activity. Compound 1 inhibited osteoclastic differentiation in a dose-dependent manner without cytotoxicity. In addition,the inhibitory mechanisms of compound 1 were investigated. Compound 1 induced a clear reduction in the expressions of NFATc1 and c-Fos upon RANKL stimulation. In summary, compound 1 from sea mustard showed antiosteoporotic potential.
순무(Brassica rapa) 뿌리로부터 이차대사산물의 분리 및 동정
방면호,한민우,정해곤,정태숙,최명숙,이경태,백남인,이대영 한국식물생명공학회 2009 식물생명공학회지 Vol.36 No.1
In order to identify secondary metabolites, the root of Brassica rapa was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and H2O. From the EtOAc and n-BuOH fractions, four secondary metabolites were isolated through the repeated silica gel and octadecyl silica gel (ODS) column chromatographies. From the result of spectroscopic data including NMR and MS, the chemical structures of the compounds were determined as 4-(methoxymethyl)phenol (1), α-methoxy-2,5-furandimethanol (2), phenyl-β-D-glucopyranoside (3), and 2-phenylethyl-β-D-glucopyranoside (4). They were isolated for the first time from Brassica rapa. 순무 뿌리로부터 활성 물질을 분리 동정 하기 위하여 80%MeOH 수용액으로 추출하고 이를 여과, 감압 농축하여 MeOH추출물을 얻었다. 이를 EtOAc분획, n-BuOH분획, H2O분획으로 나누었으며, EtOAc분획과 n-BuOH분획에 대해 silica gel 및 ODS columnchromatography를 실시하여 4종의 이차대사산물을 분리 정제하였다. 1H-NMR, 13C-NMR, DEPT spectrum 및 mass spectrum등을 통하여4-(methoxymethyl)phenol(1), α-methoxy-2,5-furandimethanol(2), phenyl-β-D-glucopyranoside(3) 및 2-phenylethyl-β-D-glucopyranoside(4)로구조를 결정하였다. 이 화합물들은 순무에서는 처음 분리되었다
흰씀바귀 (Ixeris dentata forma albiflora)뿌리로부터 Flavonoid 화합물의 분리
방면호,송명종,한민우,양혜정,이대영,백남인 慶熙大學校 食糧資源開發硏究所 2007 硏究論文集 Vol.26 No.2
The root of Ixeris dentata forma albiflora was extracted with 80% aqueous methanol and solvent fractionated with ethyl acetate, n-butanol and water, successively. From the ethyl acetate and n-butanol fractions, two flavonoid compounds were isolated through the repeated silica gel column chromatographies. The chemical structures were determined as luteolin (1), luteolin-7-0-βD-glucopyranoside (2) through the intεrpretation of several spectral data including 2D-NMR.