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Mucor spp L42 응유효소(凝乳酵素)의 고정화(固定化)에 관한 연구
박종래,이준염,고영숙 한국낙농학회 1986 韓國酪農學會誌 Vol.8 No.3
精製된 Mucor SPP L_(42) 凝乳酵素을 DEAE-cellulose, CM-cellulose, Sepharose 4-B와 porous glass beads의 四種의 支技劑를 使用하여 各各 다른 方法으로 固定化시킨 후 그 結果를 서로 比較하고, porous glass beads에 固定化시킨 Mucor app L_(42) 凝乳酵素의 性質을 調査하였던 바 그 結果를 要約하면 다음과 같다. 1. 乾燥된 各各의 支技劑에 結合된 酪素蛋白質의 量은 DEAE-cellulose에 24㎎, CM-cellulose에 22㎎, Sepharose 4-B에 20㎎, porous glass bead에 40㎎이었다. 2. 溶解狀態의 酵素蛋白質 10㎎에 대한 支技劑에 結合된 酵素蛋白質 10㎎의 헤모글로빈 分解力의 比率은 DEAE-cellulose는 23, CM-cellulose는 23, Sepharose 4-B는 24, Porous glass bead가 40으로 나타났다. 3. Porous glass bead에 結合된 Mucor spp L_(42) 凝乳酵素의 利用될 수 있는 pH와 溫渡에 대한 範位가 溶解性諸素에 비하여 넓어진 것으로 나타났다. Mucor spp. L_(42) milk-clotting enzyme was coupled to DEAE-cellulose, CM-cellulose, Sepharose 4-B and porous glass bead by different methods respectively. Amount of enzyme attatched to the matrixes was analysed by micro kjeldahl method. Activity of individual immobilized enzyme was compared to native enzyme. Properties of the immobilized Mucor spp. L_(42) milk-clotting enzyme on succinamidopropyl glass bead with glutaraldehyde were tested. The results abtained were summerized as follow. 1. Amount of enzyme attatched to one gram of individual dried beads was mg for DEAE-cellulose, mg for CM-cellulose, mg for Sepharose 4-B and mg for porous glass bead respectively. 2. Ratio of activity for 10mg of enzyme attatched on individual beads to 10mg on individual native enzyme was for DEAE-cellulose, for CM-cellulose, for Sepharose 4-B and for porous glass bead. 3. Ranges of available pH and temperature for immobilized Mucor spp L_(42) milk-clotting enzyme to succinamidopropyl glass bead were improved than soluble one.
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Molecular Sieve HPLC 방법에 의한 변성된 단백질들의 소화특성 비교
박종래,이준엽 한국낙농학회 1990 韓國酪農學會誌 Vol.12 No.3
단백질의 변성이 소화과정에 어떠한 차이를 나타내는가를 규명하기 위하여 β-lactoglobulin, α-lactalbumin과 bovine serum albumin을 단순한 열처리, 당과 열처리, 알카리 처리 등으로 각각의 단백질을 변성시킨후 고정화 �신, 고정화 트��신, 고정화 카이모트��신과 고정화 pronase을 통하여 단계적으로 순환시키면서 가수분해 시킨후 생성되는 peptide group등을 molecular sive HPLC방법으로 분리하여 서로 비교하였던 바, 그 결과는 다음과 같다. 1. 분자사별 HPLC그래프 상에서 변성되지 않은 β-lactoglobulin, α-lactalbumin과 bovine serum albumin은 단일한 peak를 나타내었으며 출현시간은 각각 다른 시간대에 나타났다. 2. β-Lactoglobulin, α-lactalbumin과 serum albumin은 단순 열처리, 당과 열처리, 알카리 처리를 분자사별 HPLC 그래프 상에 나타나는 peak의 위치와 수가 다르게 나타났다. 3. 신선한 단백질과 변성된 단백질들은 모두가 고정화 소화효소 장치에 가수분해 결과 생성되는 peptide group의 출현시간은 길어졌으며 그수도 많아졌다. In order to compare the digestible characteristics of native and denatured proteins, β-lactoglobulin, α-lactalbumin and bovine serum albumin were denatured by heat treatment, heat treatment in fructose and alkali treatment, individually. The native and the denatured proteins were hydrolysed by the sole immobilized pepsin, and digested by the immobilized trypsin, chymotrypsin and pronase successively and then the peptides produced by digestion were separated by molecular sieve HPLC. The results obtained were summarized as follow; 1. In molecular sieve HPLC diagrams, the native (β-lactoglobulin, α-lactalbumin and bovine serum albumin were appeared a single peak, but their retention times were different individually. 2. Denaturations of β-lactoglobulin, α-lactalbumin and bovine serum albumin by heat treatment, by heat and fructose treatment, and by alkali treatment, changed the elution time and the number of their eluted protein peak individually. 3. Digestion of native and denatured proteins on immobilized enzyme system prolonged the elution time, and increased the number of eluted peptide groups produced by hydrolysis of them. But according to the types of treatment and the kinds of protein, changes of the number of peak and of the degree of released time were appeared individually various different tendecies.
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촉매를 이용한 폐고분자 물질의 자원화 - 촉매글리콜분해에 의한 연질 폴리우레탄폼 폐기물의 재활용
박종래,김성익,김영철,박남국,서곤 ( Chong Rae Park,Seong Ick Kim,Young Chul Kim,Nam Cook Park,Gon Seo ) 한국공업화학회 1997 공업화학 Vol.8 No.6
촉매 글리콜분해공정은 에스테르 교환반응에 의해 생성된 폴리올과 carbamate 화합물을 회수하여 폴리우레탄 폼 제조에 이용하는 화학적 재활용 방법이다. 본 연구에서는 폴리우레탄 폼을 분해하기 위해 ethyleneglycol, diethyleneglycol, 1,4-butanediol을 사용하였으며, 촉매로는 금속 acetate류를 사용하였다. 촉매글리콜분해 반응온도는 180∼200℃을 범위에서 수행되었다. 반응속도는 반응시간 경과에 따른 생성물의 점도를 측정하여 조사하였으며, IR과 GPC분석을 통하여 분해 생성물의 종류와 분자량 분포를 조사하였다. 촉매 글리콜분해는 높은 온도에서 ethyleneglycol을 사용했을 때 잘되었다. K, Na,Tl acetate촉매의 활성이 좋았으며, 생성물들은 비교적 높은 함량의 아민화합물과 carbamate 화합물을 함유하고 있었다. Sr acetate와 Quinoline 촉매의 경우 반응은 다소 느리지만 폴리올의 함량이 높았고 부생성물의 함량이 낮았다. 회수플리올을 20wt%까지 첨가하여 제조한 폴리우레탄 폼의 물성이 버진 폴리올만을 사용하여 제조한 폼에 비해 인장강도, 경도, 인열강도, 압축강도 등이 좋았다. The catalytic glycolysis process is the method of chemical recycling where the polyol and carbamate compounds recovered by transesterification reaction are reused to produce new polyurethane foams. In this work, ethylene glycol, diethylene glycol, and 1,4-butanediol were used to decompose polyurethane foams and various metallic acetates were provided as catalysts. The catalytic glycolysis of polyurethane foams was taken place in the reaction temperature of 180-200℃. The reaction rates of catalytic glycolysis reaction were indicated by the viscosity of the reaction products at different reaction times. IR and GPC analysis showed the types and the molecular weight distributions of the products. The catalytic glycolysis was profitable for using ethyleneglycol at high temperature. The activities of the catalysts are suitable for K, Na, Tl acetate, and the products are composed of comparatively high-contained amine compounds and carbamate compounds. In the case of Sr acetate and Quinoline, the reaction rate was some what low.However, the content of polyol was high and the content of the side-products was low. The foams which were prepared by blending up to 20wt% of recovered polyol with virgin polyols showed better physical properties in tensile strength, hardness, tear strength, and compressive strength compared to those of polyurethane foams from virgin polyol.
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표면처리가 Torayca T300 탄소섬유의 Morphology 및 역학적 거동에 미치는 영향
박종래,임순호,Park, Jong-Rae,Im, Sun-Ho 한국섬유공학회 1996 한국섬유공학회지 Vol.33 No.11
The effect of surface treatment at high temperatures on the morphology and mechanical behavior of Torayca T300 carbon fiber is studied. It is found that the surface treatment results in a considerable change in the surface and cross-sectional morphology of the carbon fiber. Accordingly, there are sizable change in mechanical properties of the fiber: in particular, compressional failure mechanism changes from buckling to simple shear mode as the surface treatment temperature increases. There is not an apparent relationship between the surface structural parameters such as surface area, pore volume, etc. and the mechanical properties such as tensile and compressive strengths.
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