Disialoganglioside GD2의 Anti-idiotypic Antibody (Ab2)에 의해 유도된 Anti-anti-idiotypic Antibodies (Ab3)의 특성

박윤선,Park, Yoon-Sun 대한면역학회 2003 Immune Network Vol.3 No.2

Background: Disialoganglioside GD2 is a tumor-associated antigen that is overexpressed on tumor cells of neuroectodermal origin, such as melanoma and neuroblastoma. Anti-idiotypic antibodies that mimic GD2 may induce more effective immune responses than GD2 antigen itself, because they are protein antigens and are known to be able to break immune tolerance. In this study, to explore the potential of anti-idiotypic antibodies as tumor vaccines, the ability of anti-idiotypic antibodies (Ab2) to induce anti-anti-idiotypic antibodies (Ab3) that bind to the original antigen GD2 was investigated. Methods: Six monoclonal anti-idiotypic antibodies (1A8, 1G5, 2B6, 3A4, 3D6, 3H9) to monoclonal antibody M2058, which is a monoclonal antibody to GD2, were produced in mice. Three (1A8, 3A4, 3H9) of them were selected based on their ability to inhibit the binding of Ab1 to D142.34 (murine melanoma cell expressing GD2). These 3 different Ab2 were injected into rabbits, and rabbit Ab3 induced by each of them were characterized. Results: Ab3-containing sera from two rabbits immunized with 1A8, 3A4, or 3H9 bound significantly (P<0.05) to D142.34 but not to B78.96 (GD2-negative cell), and bound significantly (P<0.05) to isolated GD2 but not to GD1a. Ab3-containing sera from two rabbits immunized with 3A4 or 3H9 inhibited significantly (P<0.05) the binding of Ab1 M2058 to D142.34, and inhibited significantly (P<0.05) the binding of Ab1 M2058 to the Ab2. Conclusion: These results suggest that anti-idiotypic antibodies 3A4 and 3H9 have a potential to be used as vaccines against tumors expressing GD2 by inducing GD2-specific antibodies (Ab3).

장기간 토지피복 변화에 따른 국내 생태계서비스 간 상쇄효과(Trade-off) 분석

박윤선 ( Yoon-sun Park ),송영근 ( Young-keun Song ) 한국환경생태학회 2024 한국환경생태학회지 Vol.38 No.2

생태계서비스 상쇄 효과(Trade-off) 규명과 같이 서비스 간 상호관계를 측정하는 것은 한정된 환경자원을 관리하는 측면에서 매우 중요하다. 이에, 본 연구에서는 약 30여 년간 토지 피복이 변화함에 따라 파생된 생태계서비스 우세경향 및 증감을 파악하고, 시간의 경과에 따라 발생한 생태계서비스 상호 간 관계 변화를 추적하였다. 이를 통해 토지피복 변화와 생태계서비스 변화 간의 관계 및 지역마다 상이한 서비스 변화의 특성을 규명하였다. 연구는 생태계서비스 평가 모델인 InVEST Model을 주로 활용하였고, 평가결과를 0-1사이로 표준화한 후 차원축소기법 중 하나인 주성분 분석을 거쳐 시계열변화를 관찰하고 서비스 상호 간 관계를 파악하였다. 연구 결과, 시가화 지역 면적은 1989년에서 2019년 사이 급격하게 증가했으며, 산림은 2009년에서 2019년 사이 크게 증가하는 양상을 나타냈다. 1989년에서 2019년 사이에 생태계서비스 공급량에 있어 전국적으로 수량 공급은 13.9% 감소, 질소 저류는 10.5% 감소, 인 저류는 2.6% 증가, 탄소 저장은 0.9% 감소, 대기정화는 1.2% 증가, 서식처 질은 3.4% 감소하였다. 우리나라는 지난 30여년간 시가화 지역이 증가하고, 농경지가 감소하며, 산림이 증가하는 동안 인 저류 기능과 서식처 질 사이에 상쇄 효과를 보였다. 본 연구는 우리나라의 환경관리 정책이 도시화로 인해 하락한 생태계 질을 향상시키고 생태계서비스를 극대화하는데 기여했다는 결론을 도출하였다. 이러한 연구 결과는 정책결정자들이 지속 가능한 자연환경 보전과 생태계 서비스 제공에 중점을 둔 조림 정책을 수립하고 추진하는 데 도움을 줄 수 있다. Understanding the trade-off effect in ecosystem services and measuring the interrelationships between services are crucial for managing limited environmental resources. Accordingly, in this study, we identified the dominant trends and increases and decreases in ecosystem services derived from changes in land cover over about 30 years and tracked changes in the relationships between ecosystem services that occurred over time. Through it, we determined the relationship between land cover changes and ecosystem service changes, as well as the distinct characteristics of service changes in different areas. The research primarily utilized the InVEST model, an ecosystem service assessment model. After standardizing the evaluation results between 0 and 1, it went through principal component analysis, a dimensionality reduction technique, to observe the time-series changes and understand the relationships between the services. According to the research results, the area of urbanized regions dramatically increased between 1989 and 2019, while forests showed a significant increase between 2009 and 2019. Between 1989 and 2019, the national ecosystem service supply witnessed a 13.9% decrease in water supply, a 10.5% decrease in nitrogen retention, a 2.6% increase in phosphorus retention, a 0.9% decrease in carbon storage, a 1.2% increase in air purification, and a 3.4% decrease in habitat quality. Over the past 30 years, South Korea experienced an increase in urbanized areas, a decrease in agricultural land, and an increase in forests, resulting in a trade-off effect between phosphorus retention and habitat quality. This study concluded that South Korea's environment management policies contribute to improving ecosystem quality, which has declined due to urbanization, and maximizing ecosystem services. These findings can help policymakers establish and implement forestry policies focusing on sustainable environmental conservation and ecosystem service provision.

면역 효소법을 이용한 우리나라 주민의 혈청내 Coxiella burnetii 항원에 대한 항체의 검출

박윤선(Yoon-Sun Park),이경호(Kyoung-Ho Lee),박주영(Joo-Young Park),김수기(Soo-Kie Kim),신운섭(Woon-Seob Shin),고춘명(Choon-Myung Koh) 大韓微生物學會 1994 大韓微生物學會誌 Vol.29 No.4

<옹고집전>에 나타난 개인의 형성과 그 의미

박윤선 ( Park Yoon-sun ) 한국고전문학교육학회 2013 고전문학과 교육 Vol.26 No.-

이 논문은 <옹고집전>이 다양한 감정의 경험을 통해 미성숙한 자아의 상태였던 옹고집이 하나의 개인으로 형성되면서 이 과정에서 발견되는 미묘한 균열 즉, 개인과 사회에 대한 당연한 관계설정에의 의문이 담겨있다는 데에 그 의의가 있는 작품이다. 나를 나보다 더 잘 알고 있는 존재의 등장을 통해 억울함과 분노, 두려움을 느끼는 등 진옹이 거쳐온 감정의 경험과 자신을 객관화하여 바라보고 자기 인식을 하는 것은 그를 성숙한 자아로 이끌고 하나의 개인으로 형성하게 한다. 또한 진옹이 패배하여 공동체를 떠나는 과정에서 발견되는 미묘한 균열은 당연하게 여겼던 개인과 사회의 관계에 문제를 제기하며 개인과 사회를 대립적으로 파악하고 성장한 개인을 부각시키려는 시도가 보인다. 그러나 옹고집이 개과천선에 이르기까지의 과정이 생략되어 있어 그동안 <옹고집전>이 다른 판소리계 소설에 비해 부족하다는 평가를 받으며 그 가치를 인정받지 못한 것으로 보인다. <옹고집전>에는 당연하게 여겼던 사회와 개인의 관계설정에의 문제제기와 고민이 담겨있다. 이 고민은 현대의 개인들에게도 유효한 고민이며 의미 있는 성찰로써 이를 통해 <옹고집전>의 현대적 가치를 발견할 수 있다. 당연한 것으로 여겼던 것들에 문제를 제기하고 시대의 변화와 흐름에 반응하고 고민했던 흔적이 분명하게 발견되는 <옹고집전>을 통해 작품의 새로운 가치를 찾아내기 위한 노력이 필요하다고 생각한다. This study is a work that as OngGojip who had a state of an immature ego forms as an individual through diverse emotional experiences, subtle crack is found in this process, that is to say, question on establishment of relationships about an individual and the society that is naturally regarded is contained in a work that it has a significance. Through an appearance of an existence who knows me more than myself, Jin Ong experiences emotions such as dejection, grievance, and fear, and Jin Ong sees and perceives himself by objectifying himself, which lead him to become an individual who has a mature ego. Additionally, an attempt to highlight an grown-up individual by raising a problem on relationship between individuals and society that is reasonably regarded is a delicate crack, which is found in the process when Jin Ong leaves a community because of Jin Ong’s failure. However, since the process that OngGojip underwent to the point of becoming a new man is omitted that it seems that meanwhile OngGojipjeon is assessed as inferior, compared to other novels of pansori field and that its value is not recognized. OngGojipjeon contains concern and problem-posing on establishment of relationship between a society and individuals, which is rightly considered. This concern is a valid concern for current individuals and meaningful introspection, and through these we can find modern values. Through OngGojipjeon that include clear traces of concerns and responses to age’s change and stream and problem-posing on things that are justly regarded, I think an effort shall be made to seek new values of the work.

전략환경영향평가 시 생태계서비스 평가 결과의 활용가능성에 관한 연구

박윤선 ( Park Yoon-sun ),김충기 ( Kim Choong-ki ),이후승 ( Lee Who-seung ) 한국환경복원기술학회(구 한국환경복원녹화기술학회) 2021 한국환경복원기술학회지 Vol.24 No.2

Strategic Environmental Impact Assessment (SEA) is a decision-making process taking into account the environmental impact, economic and social impact of policies, plans, and programs at the higher stage prior to the project plan for promoting sustainable development. In this study, we analyzed the process and criteria for selecting appropriate alternatives when establishing development plan in SEA. First, the criteria for estimating changes in ecosystem services following the implementation of development project of industrial complex were presented. Second, alternative evaluations were conducted through an analysis of ecosystem service scenarios to explore suitable alternatives in Anseong. As a result, the environmental quality of selected area as the existing project site deteriorated according to the implementation of the project, and the dimensional reduction technique confirmed that the change in ecosystem service factors in project area was the optimal location. In addition, the results of the scenario assessment to explore suitable alternatives in Anseong City showed that the existing site had large capacity in terms of water quality control services (scenario 1), scenario 2 in terms of pre- conditioning services, and scenario 3 in terms of water supply services. The guidance of Ecosystem service assessment is expected to be available in decision-making of large-scale strategies (e.g., SEA) and projects by presenting more quantitative criteria for determining the adequacy and location feasibility of development plans and policy plans. This is expected to require various support, including legislation and revision of related laws, believed to be supported by advanced research.

배양조건에 따른 Staphylococcus aureus의 Methicillin 내성발현

박윤선(Yoon-Sun Park),김수기(Soo-Kie Kim),신운섭(Woon-Seob Shin),이경호(Kyoung-Ho Lee),박주영(Joo-Young Park),고춘명(Choon-Myung Koh) 大韓微生物學會 1995 大韓微生物學會誌 Vol.30 No.5

Coxiella burnetii Nine Mile strain으로부터 Plasmid DNA의 분리

박윤선(Yoon Sun Park),박경숙(Kyoung Sook Park),박주영(Joo Young Park),고춘명(Choon Myung Koh) 大韓微生物學會 1994 大韓微生物學會誌 Vol.29 No.1

사회-생태계 이론을 활용한 경기도 지역 생태계서비스 공급-수요관계 분석

박윤선 ( Park Yoon-sun ),김충기 ( Kim Choong-ki ),이재혁 ( Lee Jae-hyuck ),송영근 ( Song Young-keun ),홍현정 ( Hong Hyun-Jeong ) 한국농촌계획학회 2021 농촌계획 Vol.27 No.3

Ecosystem services play a role in promoting sustainable development by contributing to human welfare. For sustainable development, a balance between supply and demand for ecosystem services must be made. In this regard, in this study, factor analysis was performed using the results of measuring ecosystem services for the supply of ecosystem services and national statistical data representing socio-economic factors for demand for ecosystem services The results of analysis for Gyeong-gi Province are as follows. The service supply based on the result of ecosystem services was divided into the mixed service provisioning as factor1, the food provisioning as factor2, and the P retention service provisioning area as factor3. As for the demand for services based on socio-economic factors, factor1 is divided into urbanized areas, factor2 is forest development area, and factor3 is agricultural activity development area. Local governments that maintain balance were evaluated as Pocheon, Yangpyeong, Icheon, Pyeongtaek, Goyang, Suwon, Gwangmyeong, and Osan, and imbalanced local governments appeared in Gimpo, Uiwang, Anseong, and Yeoju. A management plan to maintain the balance between supply and demand of ecosystem services was suggested. The analysis method and results of this study are expected to be applicable to various local governments through regional expansion.

한국에서 사육중인 젖소의 Raw Milk로부터 Coxiella burnetii의 분리 및 배양

박주영(Joo Young Park),이원영(Won-young Lee),조상래(Sang-Nae Cho),박윤선(Yoon Sun Park),박경숙(Kyoung Sook Park),윤희정(Hee Jeong Youn),강영배(Yung-Bai Kang),고춘명(Choon Myung Koh) 大韓微生物學會 1993 大韓微生物學會誌 Vol.28 No.4

CPFR 구현을 위한 다중 에이전트 시스템 구조 설계

김창욱(Chang-Ouk Kim),김선일(Sun-Il Kim),윤정욱(Jung-Wook Yoon),박윤선(Yunsun Park) 대한산업공학회 2004 대한산업공학회지 Vol.30 No.1