녹두의 발아과정중 CA3 및 ABA 의 처리가 Peroxidase, Catalase 활성변화와 Isoperoxidase Pattern 에 미치는 영향

박우철,이상갑 한국콩연구회 1989 韓國콩硏究會誌 Vol.6 No.1

식물 생장조절제에 의한 녹두의 부위별 peroxidase, catalase 활성과 전기영동상 peroxidase 등위효소의 변화를 조사하였다. 녹두의 생육이 진행됨에 따라 하배측을 제외하고는 peroxidase 활성도는 증가하였으나 catalase 활성도는 감소하여 서로 상반된 결과를 나타내었다. 암발아시킨 녹두에 GA₃와 ABA를 각각 처리하면 peroxidase 활성도의 증가에 대하여 자엽에서만 GA₃가 ABA 보다 더 큰 영향을 끼쳤으나 catalase 활성도 변화는 ABA가 GA₃ 보다 더 큰 영향을 끼쳐 모든 부위에서 활성도를 증가시켰다. GA₃와 ABA를 혼합하여 처리하면 효소 활성도의 변화 경향은 GA₃보다 ABA에 더 큰 영향을 받았다. Peroxidase 등위효소의 수는 발아, 생육이 진행됨에 따라 모든 부위에서 계속 증가하여 활성도와는 무관하게 발아 후 6일째에 가장 많이 나타났으며, 호르몬의 처리에 따른 동위효소의 변화는 동위효소의 수에 대해서 보다는 intensity에 대하여 더 큰 영향을 받은 것으로 나타났다.

根瘤菌의 人工接種이 大豆의 몇가지 形質에 미치는 影響

朴愚喆 慶北大學校 1984 論文集 Vol.37 No.-

To elucidate the effect of Rhizobium japonicum inoculation and nitrogen application on nitrogen fixing ability of root nodules and finally on the critical character of soybean cultivars, an 11×2×2 factorial analysis experiment was conduceted using 7 species and 4 hybrids of soybean as plant materials, R. japonicum strains, 011 and 019, as inoculants and urea as nitrogen source. The results obtained were as follows: 1. Twenty four indigenous Rhizobium strains isolated from root nodules of soybean plant cultivated at experiment field in Kyungpook National University, were divided into 3 groups according to the intensity of nitrogen fixing ability among several physiological characters. The group having the highest nitrogen fixing ability included 8 strains. These strains showed the small colony size, the transparent alkaline or acidic serum in the litmus milk test and the negative reaction in the nitrite reduction test. 2. In the pot experiment, the nitrogen fixing ability and the allantoin content in the N application plot(N 8kg/10a) decreased at all the growth stages as compared with the control treatment, and the influence of N application was especially severe at the maximum flowering stage. 3. The effect of inoculation was significant at 1% level in root length, stem length and total dry matter, respectively and at 5% level in node number at maximum flowering stage, and at 1% level in stem length, pod number and total dry matter, respectively at the seed filling stage. N application had effect only on the branch numbers at the maximum flowering stage with significant difference of 5% lelvel. Among clutivars the difference was significant at 1% level in stem length and total dry matter, respectively at both maximum flowering and seed filling stage, and at 5% level in node and pod number, respectively at seed filling stage. 4. At maximum flowering stage, the nodule number and the nodule weight significantly increased at 1% level by Rhizobium inoculation, and N application increased the nodule number (p. <0.01) while it decreased the nodule weight (p.<0.05). 5. The nitrogen fixing ability at the maximum flowering stage was significantly enhanced at 1% level by the Rhizobium inocluation and N application in all the cultivars except for D_4 and JTI-800. At the seed filling stage, however, the cultivars that responded to the inocluation were Kwangkyo, D_4 and Danyup×Backchun while those responded to the nitrogen fertilizer were Milyang 5 and Backchun. In Chiquita×Milyang 5, the effect of N application on nitrogen fixing ability was higher with inoculation than with non-inoculation. All the cultivars except for those described above showed higher nitrogen fixing ability with non-inoculation plus non-N application treatment. 6. The effect of Rhizobium inoculation and nitrogen application on the 100 grain weight did not show the significant difference among the total treatment, but among cultivars 1% level of significant difference was observed. On the 100 grain weight, there was significant increase with inoculation at 1% and 5% level in Kwangkyo and Kwangkyo ×D_4, and D_4, respectively, and without inoculation at 1% level in Dangyup×Backchun. The N application brout about significant increase in the 100 grain weight at 1% level and 5% level in D_4 and Chiquita, and Danyup, respectively, while in Kwangkyo and Backchun, and Danyup×JTI-800 the 100 grain weight was reduced significantly at the level of 1% and 5%, respectively. The yield was significantly increased in treatments with inoculation and non-N application at 1% level, respectively. Among cultivars and among each treatment, there was significant difference at 5% or 1% level. Significant difference was observed among clutivars at 1% level, D_4 was the lowest and Backchun the highest in the yeild. Considering the effect on the yeild in each cultivar, with inoculation cultivars resulted in significant increase were D_4, and Kwangkyo and Backchun at 1% and 5% level, respectively, while those increased with N application were D_4 and Chiquita at 1% and 5% level, respectively. Kwangkyo, Danyup×Backchun, JTI-800 and Danyup×JTI-800 were high in yield with non-N application with significant difference of 5% or 1% level. 7. In protein content among the total treatment, the inoculation and the N application resulted in significant increase at 5% and 1% level, respectively. Rhizobium inoculation increased significantly the protein content in Backchun and Danyup×JTI-800 at 1% level, and N application showed significant increase at 5% or 1% level in all cultivars except for Danyup. Fat content had negative correlation with protein content as a whole. Inoculation significantly increased the fat content at 5% or 1% level in all cultivars except for Chiquita, Danyup×Backchun and JTI-800, wherease non-N application increased the fat content in all cultivars except for Kwangkyo×D_4. 8. Soybean cultivars hgh in affinity with inoculants were Kwangkyo, Milyang 5, Kwangkyo×D_4 and JTI-800, while those with indigenous were D_4, Backchun, Danyup×Backchun and Chiquita×Milyang 5. 9. Peroxidase isozyme bands in 11 cultivars were 10 in type. Their Rm values were 0.03, 0.1, 0.5, 0.58, 0.62, 0.7, 0.78, 0.86 and 0.93. Cultivars that showed change in peroxidase isozyme pattern by inoculation were Backchun and Danyup×Backchun, while those by N application were D_4, Chiquita, Backchun and Danyup×Backchun, while those by N application were D_4, Chiquita, Backchun and Danyup×Backchun. Those sensitive to both inoculation and N application were Kwangkyo, Kwangkyo×D_4, Backchun and Danyup×Milyang 5, Danyup, JTI-800 and Danyup×JTI-800. 10. Conculsively the critical characters in soybean investigated were influenced by Rhizobium inoculation more severely than by N application, but in some cultivars inoculation in combination with N application exerted a beneficial incluence.

벅+하프 브리지 컨버터의 동작 특성

박우철,김창선 국립7개대학공동논문집간행위원회 2003 공업기술연구 Vol.3 No.-

We considered about the efficiency of the Buck+Half bridge converter. The converter provides the effective applications for a low output voltage, a high output current and a wide input voltage. Developed the Buck+Half Bridge converter ratings are of 36~72V input and 3.3V/30A output. In Half Bridge converter, the 86~96.4% of the efficiency is measured at 100kHz switching frequency with PQI core. In the case of the synchronized Buck+Half Bridge converter, the measured efficiency is higher than that of the unsynchronized coverter. In the synchronized Buck+Half Bridge, the maximum efficiency is up to 92.3% with PQI core at 100kHz, 7A output.

根瘤菌 接種이 大豆 收量에 미치는 影響

朴愚喆 慶北大學校 새마을硏究所 1980 農村과 科學 Vol.3 No.-

根瘤菌의 接種이 根瘤數 및 收量에 미치는 影響을 大豆 奬勵品種 10種에서 調査한 結果는 다음과 같다. 1. Rizobium japonicum接種區가 無接種區보다 Nodule numbers와 Nodule fresh weight가 多少 높았으며 開花期에는 두區 共히 그數値가 最高에 達했다. 2. 收穫量과 Nodule numbers는 無接種區에서는 高度의 相關關係를 나타냈으나 接種區에서는 品種間 根瘤菌에 對한 特異한 反應을 나타냈다. 3. 接種에 依한 增收效果는 有意性은 없었으나 그效果는 明確히 認定되었다. To study the effectiveness of Rhizobium japonicum on the yields of each soybean varieties, this experiment was carried out. At the flowering stages of each soybean varieties the highest nodule numbers and the greatest fresh weight of nodule were obtained however, in generally those of soybean varieties were comperatively higher in inoculation of Rhizobium japonicum than in non inoculation. In non inoculation treatment with Rhizobium japonicum the yield of soybean showed high correlation with the nodule numbers of soybean. The increasing effectiveness of Rhizobium japonicum inoculation was evident, but the least significant difference was not found.

Ninhydrin에 依한 窒素의 吸光光度 分析法에 關한 硏究

朴愚喆 진주농과대학 1967 진주농과대학 연구논문집 Vol.- No.6

A method of the colorimetric determination of nitrogen has been developed based on the fact that NH₃-N forms a stable purple complex with ninhydrin which is suitable for spectrophotometric determination of nitrogen in the concentration range of 0.2 to 22㎍ per ml. as NH₃. The determination was carried out in the solution of pH5.5 and the absorbancy of complex was measured at 570mμwith Colemann spectrophotometer. The effects of hydrogen ion concentration, reagent concentration and stability of complex were studied.

4차 산업혁명과 미래의 상담

박우철 한국아동학회 2018 한국아동학회 학술발표논문집 Vol.2018 No.4

MR유체를 특징으로 하는 반능동형 마운트의 설계

박우철 三陟大學校 2004 論文集 Vol.37 No.-

In this paper. the semi-active mount featuring Magneto-rheological fluid(MR Fluid) is proposed MR fluid is suspension of micro sized magnetizable particles in a fluid medium, and its apparent viscosity can be vaned by the applied strength of magnetic field. When the controllable MR fluid is applied to mechanical devices. the devices provide simple. rapid-response interfaces between electronic controls and mechanical systems. The MR fluid is applied in the conventional fluid mount for improving its performance of the mount's isolation effect. A appropriate size of the MR mount is designed and manufactured on the basis of Bingham model of MR fluid. In addition, the field-dependent damping forces of MR mount are evaluated with respect to the input frequency variation.

優良室素固定菌의 探索分離 및 生理的 特性

朴愚喆,李光熙,金進鎬,李麟九,曺晋基 慶北大學校出版部 1987 慶北大農學誌 Vol.5 No.-

This experiment was conducted to search for Rhizobia with good nitrogen fixation abilities and to investigate their physiological characteristics isolated from 12 soybean cultivars and the affinities of root nodule bacteria with soybeans. The results obtained were as follows ; Based on colors, Rhizobia grown on YMA medium were divided into 3 groups, i. e., white, translucent and transparent, amounting to 60, 30 and 10%, respectively. In litmus milk reation, the strains which produced alkali, acid serum, alkaline serum and acid reached to 51, 29, 9 and 11%, respectively. Strains, S022, and S096 were slow-growers and produced alkaili, while strains, S080, S090, and S118 were fastgrowers and produced acid. The growth of root nodule bacteria on YMA medium was favorable between the initial pHs of 6.0~7.0. Glutamine, asparagine and allantoin as nitrogen sources enhanced the growth of root nodule bacteria. All the strains tested formed nodules on the soybean roots, and the strains with good symbiotic nitrogen fixation abilities that had white color, small colony, nitrate reduction abilities and no nitrite reduction abilities showed comparatively high nitrogen fixing activities. Some strains varied in nitrogen fixing activities according to soybean cultivars, and a few strains formed ineffective nodules which showed no nitrogen fixing activity.

부부상담 임상연구에서 모델중심 성과연구와 공통요인 연구에 대한 개관

박우철 한국상담학회 2016 상담학연구 Vol.17 No.2

Couple therapy as a mode of psychotherapy has gradually developed over the decades, which was possible by virtue of the development of outcome studies examining efficacy/effectiveness of treatment models. However, due to limitations of the outcomes studies couple therapy research started to evolve into a different type of approach, namely, common factors approach, which focuses more on common change mechanisms existing across treatment models. The present study delineates the trends of couple therapy clinical research by reviewing both model-centered outcome research and pan-theoretical common factors research. Specifically, the study introduces outcome studies testing efficacy/effectiveness of developed or enhanced treatment models and reviews couple therapy models which have been empirically supported by the outcome studies. Also, the study introduces the newly arising common factors paradigm and reviews relevant common factors research. Lastly, recommendations for research and training are discussed. 부부상담은 부부관계를 향상시키는 데 있어 그 효과를 입증하며 지난 수십 년간 발전해왔다. 이러한 부부상담의 발전은 개별 부부상담모델을 개발하고 그 효과를 입증한 성과연구(outcome research)의 발전이 있었기에 가능한 것이었다. 이렇게 특정 모델의 개발과 수정에 집중하던 부부상담의 임상연구는, 최근 특정 모델을 넘어 여러 모델에 공통적으로 존재하는 변화기제에 초점을 두는 공통요인(common factors)에 대한 관심으로 이어졌다. 본 연구는 이러한 부부상담 임상연구의 발전양상을 모델중심 성과연구와 범모델적 공통요인 연구를 중심으로 개관한다. 구체적으로, 본 연구는 개별모델의 효과를 측정하는 성과연구를 소개하고 성과연구를 통해 과학적 효과를 입증한 부부상담모델들을 정리한다. 나아가 공통요인 패러다임을 소개하고 관련 연구를 소개한다. 마지막으로 본 연구는 부부상담 임상연구의 발전 동향과 국내 부부상담의 현실을 고려하여 연구, 교육/훈련에서의 미래 제언을 제시한다.

EMP 위협과 방호시설 시험평가

박우철 대한전자공학회 2014 전자공학회지 Vol.41 No.7