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박영호,김선봉,염동민,이태기,박영범,도정룡,김외경 한국수산학회 1993 한국수산과학회지 Vol.26 No.5
수산자원의 고도이용과 기능특성 해명을 위한 연구의 일환으로 수산식품 중에 존재하는 생리활성물질을 검색하기 위하여 전통수산발효식품의 하나인 정어리 어간장의 숙성에 따른 ACE 저해작용 및 gel 여과에 의한 활성획분의 분리 및 그 특성을 살펴본 결과는 다음과 같다. 1. 정어리 어간장의 숙성에 따른 5% TCA 가용성 peptide-nitrogen의 생성은 전반적으로 숙성기간의 경과와 더불어 증가하다가 감소하는 경향을 나타내었으나 잔사를 이용한 정어리 어간장의 경우는 숙성과 더불어 계속 감소하는 경향을 나타내었다. ACE 저해작용 또한 peptide-nitrogen의 생성과 유사한 경향을 나타내었으며, 코오지 첨가구가 무첨가 경우보다 다소 높은 것으로 나타났으나 찬사를 이용한 경우는 오히려 코오지 무첨가 경우가 약간 높은 것으로 나타났다. 2. 정어리 어간장의 ACE 저해효과는 첨가량에 따라 급격히 증가하는 것으로 나타났으며 특히 육으로 만든 정어리 어간장의 ACE 저해효과는 코오지 첨가구의 경우가 무침가구에 비하여 높은 것으로 나타났다. 3. ACE 저해작용에 대한 가열의 영향은 다소 차이는 있으나 거의 변화가 없는 것으로 나타났다. 4. IC_(50)은 코오지 무첨가 잔사, 육 및 전이체로 만든 정어리 어간장의 경우는 각각 125㎍, 200㎍ 이상 125㎍으로 나타났으며, 코오지 첨가구의 경우는 각각 140㎍, 100㎍ 및 135㎍으로 나타났다. 5. Gel 여과에 의한 정어리 어간장의 ACE 저해 작용을 가지는 획분중 획분 C가 가장 우수한 것으로 나타났으며, 이 때의 분자량은 1,400전후인 것으로 나타났다. 6. 획분 C의 아미노산 조성은 Glu, Ala, Lys 및 Leu의 함량이 높은 것으로 나타났다. Fish sauces prepared from sardine, Sardinops melanosticta were tested for inhibitory activity against angiotensin-I converting enzyme(ACE). Three kinds of fish sauces were prepared from scrap(S), meat(M) and round(R) of sardine, respectively. ACE inhibitory activity of sardine sauce S and R decreased with the elapse of fermentation period, whereas that of sardine sauce M increased to 30 days and thereafter decreased. ACE inhibitory activity of sardine sauce M fermented with koji was higher than that without koji. And occurrence of 5% TCA soluble peptide-nitrogen was similar to tendancy of the ACE inhibitory activity. The ACE inhibitory activity increased with an increment of amounts added and was stable at heat treatment in boiling water bath for 5hrs. IC_(50) (Amounts of inhibitors need for 50% inhibition) of the sardine sauce S, M and R fermented with(without) koji during 90 days was 125㎍(140㎍), 200㎍(100㎍) and 125㎍(135㎍), respectively. From the profiles of fractionation of the sardine sauce R fermented without koji for 90 days, the molecular weight of most active fraction was about 1,400 and the amino acids of Glu, Ala, Leu and Lys were found in abundance.
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朴榮浩 釜山水産大學校 1973 釜山水産大學 硏究報告 Vol.13 No.1
In the present study, the effect of storage condition, particularly, high humidity to the changes in chemical components of dried lavers was investigated and some characteristic patterns of the changes were discussed. Dried lavers, Porphyra yezoensis Ueda, were stored at 20� under two different levels of humidity, that were 78% and lower than 7% as relative humidity, respectively. And the content of pigments, organic acids, sugars, and fatty acids were analyzed at 10,25 and 45 day intervals during the storage. Total acidity was also determined. Degradation of chlorophyll a was faster than other pigments such as carotenoids and phycobilins under high humidity. For instance, after 45 day storage, more than two-fold of chlorophll a was retained in low humidified sample compared with that in the case of high humidity whereas a remarkable difference in retention rate of carotenoids or phycobilins was not detected. phycobilins showed high stability under the condition of high humidty, particularly, in phycoerythrin than in phycocyanin. Eight kinds of non-volatile organic acid namely pyruvic, lactic, malonic, fumaric, oxalic, malic, succinic, and citric acid were gas chromatographically identified. The stability of theses acids appeared quite differently by storage humidity. The acids like oxalic, malic, and succinic, did not show an outstanding change under low humidity even after 45 day storage while both oxalic and malic acids remarkably decrased under high humidity and succinic acid contrary increased under the same condition. Citric acid, however, decreased under both cases of humidity. The total amount of volatile acid tended to increase throughout the storage under both high and low humidity, but the rate of increase was relatively greater in case of high humidity than that of low humidity. On the other hand, the same tendedcy was observed in change of total acidity in which the value tended to increase under the high humidity while that remained steady under the low humidity. In the free amino acid composition of dried lavers, alanine,glutamic and aspartic acid were dominant in content whereas such amino acids as methionine, cystine, histididine, comparatively low. It is noteworthy that the content of taurine was tremendously high as an amino acid related compound. During the storage, a significant change in the total amino acid level and individual variation was not detected. In another aspect of storage stability of chemical components, glucose remained with negligible change under both storage conditions and galactose increased rapidly under the high humidity while maintained a steady level under the low humidity. On the contrary, the floridoside decreased rapidly under the high humidity whereas slowly under the low. In the tendecy of strorage stability of fatty acid, tended to decrease in the low humidified material. Under high humidity, ?? acid appeared stable while ?? acid considerably increased. The total fatty acid level which was consisted of 14 kinds of fatty acid seemed to increase in high humidified material and to decrease in the low humidified material.
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박영호,현상일 한국물리학회 2019 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.75 No.8
We use an eigenmode analysis to investigate normal mode vibrations of hexagonal boron nitride (h-BN) and of various types of graphene and h-BN composites. The vibrational spectrum of pristine h-BN structure was a rescaled one of pristine graphene. The vibrational spectra of graphene and h-BN composites with zigzag and with armchair boundaries were just mixtures of the vibrational spectra of the pristine structures of graphene and h-BN, respectively. The vibrational spectrum of h-BN doped with a graphene quantum dot was similar to that of h-BN, and the vibrational spectrum of graphene doped with a small piece of boron nitride was similar to that of graphene. We found that the resemblance of the vibrational spectra was closely related to the similarities of the lattice structures. Using the amplitude analysis, we were able to identify the eigenmodes of the various composites as motions of nodes in the graphene region or in the h-BN region or at the interfacial boundaries between them.
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