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      • KCI등재후보

        기능성 흑찰거대배아미 조생종 벼 품종 ‘눈큰흑찰’

        박동수(Dong-Soo Park),황운하(Un-Ha Hwang),박수권(Soo-Kwon Park),이종희(Jong-Hee Lee),한상익(Sang-Ik Han),조준현(Jun-Hyeon Cho),이지윤(Ji-Yoon Lee),오성환(Seong-Hwan Oh),장기창(Ki-Chang Jang),서우덕(Woo-Duck Seo),신동진(Dong-Jin Shin) 한국육종학회 2015 한국육종학회지 Vol.47 No.1

        Nunkeunheugchal (registration No. 01-0001-2014-4), a black waxy giant embryo rice cultivar, was developed by the rice breeding team of National Institute of Crop Science (NICS), RDA in 2012. This cultivar was derived from the cross between get and Josaengheugchal in 2004/2005 winter season, and selected by a promising line, YR25277-B-B-314-2, was selected and designated as the line of Milyang263 in 2009. The local adaptability test of Milyang263 was carried out at four locations from 2010 to 2012 and it was named as Nunkeunheugchal. This variety is a early maturity cultivar. It has 65 cm in culm length and 72 spikelets per panicle, and 1,000 grain-weight of brown rice is 17.8 g which is less than that of Josaengheugchal. This variety is resistant to leaf blast, but susceptible to bacterial blight, neck blast, virus disease and insect pest. The yield potential of Nunkeunheugchal was about 3.54 MT/ha as brown rice at ordinary fertilizer level in local adaptability test for three years. Nunkeunheugchal possesses benefits to rice consumers because of high amounts of GABA, anthocyanin, calcium and iron. This variety would be adaptable to the paddy field of middle and southern plain region of Korea.

      • KCI등재

        Development of Marker-free Transgenic Rice for Increasing Bread-making Quality using Wheat High Molecular Weight Glutenin Subunits (HMW-GS) Gene

        Soo-Kwon Park(박수권),DongJin Shin(신동진),Woon-Ha Hwang(황운하),Se-Yun Oh(오세윤),Jun-Hyun Cho(조준현),Sang-Ik Han(한상익),Min-Hee Nam(남민희),Dong-Soo Park(박동수) 한국생명과학회 2013 생명과학회지 Vol.23 No.11

        고분자 글루테닌 서버유닛(high molecular-weight glutenin subunit, HMW-GS)은 밀의 가공적성을 결정하는데 중요한 역할을 수행한다. 우리는 Agrobacterium 동시 형질전환법을 이용하여 한국 밀 품종인 ‘조경’으로부터 밀 HMW-GS을 암호화하는 Glu-1Bx7 유전자를 가지는 marker-free 형질전환 벼를 생산하였다. Glu-1Bx7 유전자의 종자 특이적 발현을 위하여 밀 Glu-1Bx7 유전자 자체 프로모터를 벡터 내에 삽입하였다. 동시 접종을 위해서 오직 Glu-1Bx7 유전자와 hygromycin phosphotransferase II (HPTII) 저항성 유전자만으로 구성된 두 종류의 발현 카셋트를 독립적으로 Agrobacterium EHA105에 도입하였고, Glu-1Bx7와 HPTII가 도입된 각각의 EHA105 Agrobacterium을 3:1 비율로 혼합하여 벼 캘러스에 접종하였다. 216개의 HPTII 저항성 형질전환체 중에서 벼 게놈에 Glu-1Bx7과 HPTII가 모두 삽입된 24개의 형질전환 라인을 획득하였다. Glu-1Bx7와 HPTII가 벼 게놈에 도입된 것을 Southern blot을 통해서 다시 확인하였다. 형질전환 벼 T₁ 세대의 종자에서 밀 Glu-1Bx7 유전자가 전사와 번역되어 오직 Glu-1Bx7만을 가지는 marker-free 식물체를 T₁ 세대에서 성공적으로 선발할 수 있었다. High-molecular weight glutenin subunits (HMW-GS) have been shown to play a crucial role in determining the processing properties of the wheat grain. We have produced marker-free transgenic rice plants containing a wheat Glu-1Bx7 gene encoding the HMG-GS from the Korean wheat cultivar ‘Jokyeong’ using the Agrobacterium-mediated co-transformation method. The Glu-1Bx7-own promoter was inserted into a binary vector for seed-specific expression of the Glu-1Bx7 gene. Two expression cassettes comprised of separate DNA fragments containing only Glu-1Bx7 and hygromycin phosphotransferase II (HPTII) resistance genes were introduced separately to the Agrobacterium tumefaciens EHA105 strain for co-infection. Each EHA105 strain harboring Glu-1Bx7 or HPTII was infected to rice calli at a 3:1 ratio of Glu-1Bx7 and HPTII, respectively. Then, among 216 hygromycin-resistant T0 plants, we obtained 24 transgenic lines with both Glu-1Bx7 and HPTII genes inserted into the rice genome. We reconfirmed integration of the Glu-1Bx7 gene into the rice genome by Southern blot analysis. Transcripts and proteins of the wheat Glu-1Bx7 were stably expressed in the rice T₁ seeds. Finally, the marker-free plants harboring only the Glu-1Bx7 gene were successfully screened at the T₁ generation.

      • KCI등재

        남자대학생의 카바디 운동이 대사호르몬 및 면역기능에 미치는 영향

        박동수(Dong-Soo Park),양상훈(Sang-Hoon Yang) 한국발육발달학회 2018 한국발육발달학회지 Vol.26 No.4

        The purpose of this study was to investigate the effect of a Kabaddi exercise to metabolic hormone and immune function in male college students. During the experiment 14 men were selected and tested. The subjects were randomly assigned to a control (CON: n=7) or a raining group (TR: n=7) group. A total of 2 blood samples were collected before and after 12 weeks of exercise. First, insulin was significantly decreased while glucagon was increased but did not show any significant change in the strength group. On the other hand, insulin was increased and glucagon was decreased, indicating no significant change in the control group. Second, there were significant increases in the neutrophils and lymphocytes, while there was increases in the monocyte counts but not significant change. On the other hand, neutrophils and lymphocytes were increased, while monocyte counts was decreased but did not show any significant changes in the control group. Third, immunity globulin was significantly increased in IgA, while it was increased in IgG and IgM but not significant. On the other hand, there were increases in IgA, IgG and IgM but not significant changes in the control group. Based on the results from the experiment, the Kabaddi exercise program resulted in a decrease in insulin and an increase in neutrophils and lymphocytes. It is expected to enhance the control of blood sugar, speed of muscle recovery and increase of immunity. It also improved metabolism hormone and immune function.

      • KCI등재

        키즈 필라테스와 수영운동이 유소년의 신체구성 및 체력에 미치는 효과

        박동수(Dong-Soo Park),김송은(Song-Eun Kim) 한국발육발달학회 2017 한국발육발달학회지 Vol.25 No.4

        The purpose of this study is to scientifically find out how kids pilates and swimming exercise program during 12 weeks influences body composition and physical strength of primary school students and provide material about the formation of physical strength. A total of 36 members of 17 kids pilates exercise group and 19 swimming exercise group were surveyed, respectively, to measure body composition(body weight, BMI) and physical strength(Cardiorespiratory endurance, muscular strength, muscular endurance, flexibility). We have come to a conclusion as follows : First, after kids pilates and swimming exercise during 12 weeks, weight and BMI of body composition seemed to decrease but there is no significant difference. Secondly, after kids pilates exercise during 12 weeks, muscular strength(p<.001), muscular endurance(p<.001), and flexibility(p<.01) etc. of physical strength showed significant increases but there is no significant difference in Cardiorespiratory endurance. Third, after swimming exercise during 12 weeks, Cardiorespiratory endurance(p<.01), muscular strength(p<.001), muscular endurance(p<.001), and flexibility(p<.001) etc. of physical strength were shown to be improved meaningfully. When combined with the results, kids Pilates and swimming exercise program have improved the physical factors.

      • KCI등재

        규칙적인 생리주기를 갖는 2,879명의 한국 여성에서의 나이에 따른 anti-Mullerian hormone 수치 분포에 관한 연구

        박동수 ( Dong Soo Park ),김영주 ( Young Ju Kim ),김미경 ( Mi Kyoung Kim ),이지은 ( Jee Eun Lee ),김미라 ( Mi La Kim ),성석주 ( Seok Ju Seong ),윤보성 ( Bo Sung Yoon ),송태종 ( Tae Jong Song ) 대한산부인과학회 2012 Obstetrics & Gynecology Science Vol.55 No.12

        목적 불임치료센터를 방문한 규칙적인 생리주기를 갖는 한국 여성에서 나이에 따른 anti-Mullerian hormone (AMH) 수치 분포를 알아보고자 하였다. 연구방법 2010년 5월부터 2011년 4월까지, 생리주기 2일 또는 3일에 혈청내 AMH 검사를 시행받은 25세에서 45세 사이의 여성 2,879명을 대상으로 후향적인 연구를 시행하였다. 결과 환자의 평균 나이는 34.2 ± 4.3세, 평균 AMH 값은 3.54 ± 2.66 ng/mL, 중위수는 2.9 ng/mL이었다. 혈청내 AMH 값은 나이와 관련된 분포를 보였으며, 나이의 증가에 따라 음의 상관관계를 가졌다. 다양한 회귀모형 중, 2차 모형이 AMH와 나이와의 관계를 기술하는 데 가장 적합한 모형이었다(logAMH = -1.442 + 0.225×age-0.004×age2). 결론 본 연구의 데이터는 규칙적인 생리주기를 갖는 한국의 불임 여성의 혈청내 AMH 값의 참고치를 보여준다. 이는 난소예비능에 대한 중요한 정보로써 불임환자 및 임상의사에게 사용될 수 있을 것이다. Objective To determine the age-related distribution of anti-Mullerian hormone (AMH) in Korean women with regular menstruation who visited fertility center. Methods Between May 2010 and April 2011, serum AMH levels on day 2-3 of a spontaneous menstrual cycle were evaluated in 2,879 women of reproductive age ranging from 25 to 45 years old, retrospectively. Results The mean age was 34.2 ± 4.3 years. The overall mean AMH value was 3.54 ± 2.66 ng/mL (median, 2.9 ng/mL). Serum AMH level showed an age-dependent distribution and correlated negatively with age. Among multiple regression models, the quadratic model was most appropriated to describe the relationship between AMH and age (logAMH = -1.442 + 0.225 × age-0.004 × age2). Conclusion Our data showed reference values of serum AMH in Korean infertile women with regular menstruation. These values can be used as an important information of ovarian reserve for infertility patients and clinicians.

      • KCI등재

        감성노동의 개념화와 선행요인

        박동수(Dong-Soo Park),전명구(Myeong-Gu Jeon),정성한(Sung-Han Jeong) 한국인사관리학회 2005 조직과 인사관리연구 Vol.29 No.4

        본 연구의 목적은 감성노동의 개념화를 제시하고, 이에 상응하는 개념들을 조작화하며, 그 개념화에 기초하여 감성노동의 선행요인들을 실증적으로 검정하는 것이다. 감성노동에 대한 선행연구에 기초하여 감정노동은 3가지 차원, 즉 가식행위, 내면화행위 및 진실행위로 개념화되었다. 또한, 본 연구는 감성노동의 선행요인을 세 가지 집단, 즉 상호작용의 한 당사자인 종업원 개인특성, 또 다른 당사자인 고객특성, 그리고 종업원과 고객간 상호작용이 일어나는 상황으로서 조직 및 직무특성으로 분류하였다. 이에 기초하여 감성노동의 선행요인에는 종업원 개인특성으로서 긍정적 및 부정적 정서성, 고객특성으로서 고객태도, 그리고 조직 및 직무특성으로서 감정표현규칙, 고객접촉빈도, 상호작용 지속시간, 감정표현의 다양성이 포함되었다. 본 연구의 결과는 감성노동이 3가지 차원의 구조를 가지고 있다는 것을 지지하였다. 또한, 본 연구결과는 여러 선행요인들이 각 감성노동전략에 상이한 영향을 미치고 있다는 것을 보여주고 있다. 이러한 결과에 대한 이론적 및 실무적 시사점들이 논의되었다. The purpose of this study is three-folded: to present a conceptualization of emotional labor, to develop a corresponding operationalization of the concepts, and to examine some antecedents of emotional labor. Based on previous studies on emotional labor, we conceptualized emotional labor in terms of three dimensions: surface acting, deep acting, and genuine expression. This study also categorizes antecedents of emotional labor dimensions into three groups: employee characteristics as one party, customer characteristics as another party involved in interaction, and organizational and job characteristics as situations involved in the interacting between employees and customers. The antecedents include positive/negative affectivity as employee characteristics, customer attitude as customer characteristics, emotional display rules, frequency and duration of interaction, and diversity of emotional display as job and organizational characteristics. Results show some support for a three-dimensional structure of emotion labor. Results also show that several antecedents have different effects on each emotional labor dimension. Theoretical and practical implications from the outcomes are discussed.

      • KCI등재

        다문화 아동의 유아기 문화경험 만족도, 문화참여 동기, 문화참여 지속 정도와 문화인식 간의 구조적 관계

        박동수(Park, Dong-Soo),조혜영(Jo, Hye-Young) 학습자중심교과교육학회 2018 학습자중심교과교육연구 Vol.18 No.22

        본 연구는 다문화 아동의 유아기 문화경험 만족도, 문화참여 동기, 문화참여 지속정도와 문화인식 간의 구조적 관계를 알아보는데 목적이 있다. 이를 위해 2018년 8월부터 9월까지 전라남도에 거주하는 아동 110명을 대상으로 구조방정식을 이용하여 영향력과 부트스트랩으로 매개효과를 확인하였다. 연구결과를 제시하면 첫째, 다문화 아동의 유아기 문화경험 만족도, 문화참여 동기, 문화참여 지속 정도와 문화인식 간에 정적 상관관계가 나타났다. 둘째, 다문화 아동의 유아기 문화경험 만족도는 문화인식에 직접적인 영향을 주진 않지만, 문화참여 동기가 완전 매개하는 것으로 나타났다. 유아기 문화경험 만족도는 문화참여 지속 정도에 직접적으로 영향을 주진 않지만, 문화참여 동기가 완전 매개하여 문화인식에 영향을 주는 것으로 나타났다. 이는 다문화 아동이 문화에 대해 긍정적으로 인식하기 위해서는 문화참여 동기가 중요한 요인임을 나타나는 결과이다. 도출된 결과를 바탕으로 다문화 아동의 문화생활을 활성화 시킬 수 있는 방안 모색과 문화정책 수립 시 방향성을 제시한다는 점에서 의의를 가질 수 있다. The purpose of this study is to investigate the structural relationship among satisfaction on cultural experience in early childhood, motivation for cultural participation, continuance in cultural participation and cultural perception of multicultural children. For this purpose, the data responded August to September 2018 by 110 children who live in Jeollanam-do province were analyzed using the structural equation model and mediation effects were examined by bootstrapping. The results of this study are as follows. First, there were positive correlations among satisfaction on cultural experience in early childhood, motivation for cultural participation, continuance in cultural participation and cultural perception of multicultural children. Second, although the satisfaction of cultural experience in early childhood did not directly affect the continuance in cultural participation, it was found that motivation for cultural participation was completely mediating between them. The satisfaction of cultural experience in early childhood indirectly affect the continuance in cultural participation mediated by motivation for cultural participation. These results of this study suggest that motivation for cultural participation is major factor for multicultural children to recognize culture positively. This study is meaningful that the ways to activate cultural life of multicultural children and direction of cultural policy could be suggested based on the results.

      • KCI등재

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