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황소개구리 (Rana catesbeiana)와 참개구리 (Rana nigromaculata) 정자의 미세구조
이정훈,박기룡,Lee, Jung-Hun,Park, Ki-Ryong 한국현미경학회 2005 Applied microscopy Vol.35 No.4
The sperm morphology of two Korean frogs, Rana catesbeiana and R. nigromaculata (Ranidae) was studied with the light microscope, scanning and transmission electron microscopes. Sperm heads of the R. catesbeiana and R. nigromaculata were long-cylinder shapes. In the total length of R. nigromaculata sperm ($51.2{\mu}m$) was longer than those of R. catesbeiana ($44.6{\mu}m$). Both R. catesbeiana and R. nigromaculata had the long head and principal piece, and short middle piece, but the neck was undeveloped. The axonemal structures contain a 9+9+2 arrangement of microtubules. The number of mitochondria in middle piece have less than mammalian sperm, which irregular surrounded the axone of the middle piece. In conclusion, R. catesbeiana and R. nigromaculata shared many similar features in sperm heads, neck, middle piece, and a site of mitochondria and axial structure, and it suggests that these species should be closely related.
대극과 대극속 Zigophyllidium절의 화분분류학적 연구
이은덕,박기룡,Lee, Eun-Deok,Park, Ki-Ryong 한국현미경학회 2006 Applied microscopy Vol.36 No.3
Using light and scanning electron microscopy morphological studies of pollens were conducted to determine the sectional boundary and the relationships among eight species of section Zygophyllidium and its five related species of subg. Poinsettia. Pollen characters of 13 species were described, and their diagnostic keys were presented here. Based on the sculpturing patterns and the results of numerical analysis using 10 pollen characters 13 species were divided into four Pollen types: type I, having micro-reticulate shaped pollen (E. hexagona, E. lagunensis, E. hexagonoides and E. bilobata), type II, having reticulate shaped pollen (E. delicatula, E. extipulata, E. dentata, E. heterophylla, E. pulcherrima and E. cyathophora), type III, micro-reticulate shaped pollen with platten muri (E. lacera), and type IV, haying foveolate shaped pollen grains (E. chersonesa and E. eriantha). The results of pollen morphological studies suggest that the sect. Zygophyllidium should be delimited based on only four species, E. hexagona, E. lagunensis, E. hexagonoides and E. bilobata, E. delicatural and E. exstipulata of sect. Zygophyllidium must be transferred to subg. Poinsettia based on pollen morphology. E. eriantha and E. chersonesa having forveolate sculpturing pattern of pollen grains seem to be closely related with species from subg. Agaloma, and additional studies of these species are needed to determine the species relationships within Agaloma group.
대극속 Anthacantha절(대극과)과 근연분류군의 종자형태
나희정,박기룡,Na, Hee-Jung,Park, Ki-Ryong 한국현미경학회 2010 Applied microscopy Vol.40 No.4
The seeds of 16 species from sect. Anthacantha and related taxa were examined using light and scanning electron microscopy to illucidate the boundary of the section and their relationship among species. Using six qualitative characters clustering analyses were conducted, and three types were recognized. Type I including species from sect. Anthacantha+sect. Meleuphorbia is characterized by the ovate shape, rounded at base and smooth along the ventral line. Type II including species from sect. Medusae+sect. Treisia-1 is squared in shape and are tuberculate. Tubercles are prominent along the ventral line. Type III (sect. Treisia-2 group) is mostly rounded, and as in type II, tubercles are prominent along the ventral line, but the unique crestae consisting of the grouping testa cells differed from the surface patterns of Types I and II. Based on the seed morphology, sects. Anthacantha and Meleuphorbia are closely related, which is well supported by the results from molecular and pollen morphological studies. Besides, sects. Treisia and Medusae are not closely related in terms of seed characters, and this is not consistent with the results of recent molecular studies.